Journal: Infection Prevention in Practice

Article Title: A novel method of simulated-use surface disinfection efficacy testing as Phase 3 Step 1 approach

doi: 10.1016/j.infpip.2026.100511

Figure Lengend Snippet: Residual microbial load (black) of MRSA and VRE isolates and microbicidal (white) and mechanical effect (grey) of disinfectant A against a mixed inoculum of MRSA and VRE in a simulated-use test on ABS plates ( N = 6). The two isolates were counted and shown in the graph separately and together. The microbicidal and mechanical effects were calculated based on the recovery in the drying control Dct30. For VRE, the drying control Dct30, the residual microbial load on test fields 1-4 as well as the initial cell count were lower than for MRSA. The mechanical and the microbicidal effects were also slightly lower for VRE (1.55 ± 0.11 and 0.75 ± 0.23 log10) than for MRSA (1.62 ± 0.06 and 0.86 ± 0.24 log10). MRSA, methicillin-resistant Staphylococcus aureus ; VRE, vancomycin-resistant Enterococcus faecium .

Article Snippet: Test surfaces were contaminated with either i) a clinical isolate – methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE) or Acinetobacter baumannii , ii) the reference strain Staphylococcus aureus ATCC 6538 or iii) a mixed inoculum consisting of MRSA and VRE.

Techniques: Control, Cell Characterization