mrpa  (ATCC)

Journal: Bioactive Materials

Article Title: An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers

doi: 10.1016/j.bioactmat.2024.11.029

Figure Lengend Snippet: Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

Article Snippet: We selected MRSA (ATCC 43310) and MRPA (ATCC 27853) as test bacteria.

Techniques: Zeta Potential Analyzer, Incubation, Staining

Journal: Materials Today Bio

Article Title: Inhaled predatory bacteria-loaded large porous microspheres to eradicate drug-resistant Pseudomonas aeruginosa from the lung

doi: 10.1016/j.mtbio.2025.101562

Figure Lengend Snippet: Pulmonary respiratory functions of the mice after different treatments. Pulmonary ventilation function indexes TV (A) and EF50 (B), and pulmonary gas transfer function indexes SpO 2 (C) and drop times (D) of the mice in different groups. Meropenem-WTPA, B. bacteriovorus -WTPA, and BPM-WTPA represent the mice with WTPA pneumonia treated with meropenem solutions, B. bacteriovorus suspensions, and BPM suspensions, respectively. Meropenem-MRPA, B. bacteriovorus -MRPA, and BPM-MRPA represent the mice with MRPA pneumonia treated with meropenem solutions, B. bacteriovorus suspensions, and BPM suspensions, respectively. n = 3, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, no significance.

Article Snippet: An aliquot (25 μL) of 1 × 10 6 CFU/mL MRPA or wild-type P. aeruginosa (WTPA) suspensions was sprayed into the lung of mice through the trachea using the MicroSprayer®/Syringe Assembly including an IA-1B MicroSprayer® Aerosolizer and an FMJ-250 High-Pressure Syringe (Penn-Century Inc., PA, USA).

Techniques:

Journal: Materials Today Bio

Article Title: Inhaled predatory bacteria-loaded large porous microspheres to eradicate drug-resistant Pseudomonas aeruginosa from the lung

doi: 10.1016/j.mtbio.2025.101562

Figure Lengend Snippet: Antibacterial and anti-inflammatory effects of BPMs on the mice with pneumonia. (A) The P. aeruginosa numbers in the mouse lungs in the different groups. Expressions of TNF-α (B) and IL-6 (C) in the lung tissues of the mice in the different groups. The images of immunohistochemical staining of NF-κB (D) and NLRP1 (E) in the lung tissues of the mice in the different groups. Meropenem-WTPA, B. bacteriovorus -WTPA, and BPM-WTPA represent the mice with WTPA pneumonia treated with meropenem solutions, B. bacteriovorus suspensions, and BPM suspensions, respectively. Meropenem-MRPA, B. bacteriovorus -MRPA, and BPM-MRPA represent the mice with MRPA pneumonia treated with meropenem solutions, B. bacteriovorus suspensions, and BPM suspensions, respectively. n = 3, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, no significance.

Article Snippet: An aliquot (25 μL) of 1 × 10 6 CFU/mL MRPA or wild-type P. aeruginosa (WTPA) suspensions was sprayed into the lung of mice through the trachea using the MicroSprayer®/Syringe Assembly including an IA-1B MicroSprayer® Aerosolizer and an FMJ-250 High-Pressure Syringe (Penn-Century Inc., PA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Materials Today Bio

Article Title: Inhaled predatory bacteria-loaded large porous microspheres to eradicate drug-resistant Pseudomonas aeruginosa from the lung

doi: 10.1016/j.mtbio.2025.101562

Figure Lengend Snippet: BPMs alleviate injury and apoptosis in lung tissues of mice with pneumonia. (A) H&E staining images of lung tissues in different groups of mice. (B) TUNEL staining images of lung tissues in different groups of mice. Meropenem-WTPA, B. bacteriovorus -WTPA, and BPM-WTPA represent mice with WTPA pneumonia treated with meropenem solutions, B. bacteriovorus suspensions, and BPM suspensions, respectively. Meropenem-MRPA, B. bacteriovorus -MRPA, and BPM-MRPA represent mice with MRPA pneumonia treated with meropenem solutions, B. bacteriovorus suspensions, and BPM suspensions, respectively.

Article Snippet: An aliquot (25 μL) of 1 × 10 6 CFU/mL MRPA or wild-type P. aeruginosa (WTPA) suspensions was sprayed into the lung of mice through the trachea using the MicroSprayer®/Syringe Assembly including an IA-1B MicroSprayer® Aerosolizer and an FMJ-250 High-Pressure Syringe (Penn-Century Inc., PA, USA).

Techniques: Staining, TUNEL Assay