Structured Review

Thermo Fisher mrna
Protein tyrosine phosphatase μ (PTPμ) <t>mRNA</t> and protein levels are decreased by short-hairpin RNA <t>(shRNA).</t> (A) Lysates from U-87 MG cells infected with control or PTPμ shRNA lentivirus were immunoblotted for PTPμ using the intracellular antibody SK-18. PTPμ shRNA reduced PTPμ protein levels in comparison to uninfected and control shRNA-infected cells. Vinculin was used as a loading control. Densitometry values for PTPμ and vinculin are shown under each lane and were normalized to parental cells. The numbers on the left side of the immunoblot correspond to the molecular weight in kilodaltons. (B) RNA from U-87 MG cells expressing control or PTPμ shRNA was analyzed by reverse transcriptase PCR for PTPμ, PTPρ, PTPκ, and PCP-2 (PTPλ) expression. PTPμ mRNA levels were decreased by shRNA targeting PTPμ. PTPκ and PCP-2 mRNA levels were unaffected; PTPρ was not expressed. The numbers on the left side of the gel represent the PCR product size in base pairs.
Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mrna/product/Thermo Fisher
Average 99 stars, based on 2309 article reviews
Price from $9.99 to $1999.99
mrna - by Bioz Stars, 2020-01
99/100 stars

Images

1) Product Images from "PTP? suppresses glioma cell migration and dispersal"

Article Title: PTP? suppresses glioma cell migration and dispersal

Journal: Neuro-Oncology

doi: 10.1215/15228517-2009-019

Protein tyrosine phosphatase μ (PTPμ) mRNA and protein levels are decreased by short-hairpin RNA (shRNA). (A) Lysates from U-87 MG cells infected with control or PTPμ shRNA lentivirus were immunoblotted for PTPμ using the intracellular antibody SK-18. PTPμ shRNA reduced PTPμ protein levels in comparison to uninfected and control shRNA-infected cells. Vinculin was used as a loading control. Densitometry values for PTPμ and vinculin are shown under each lane and were normalized to parental cells. The numbers on the left side of the immunoblot correspond to the molecular weight in kilodaltons. (B) RNA from U-87 MG cells expressing control or PTPμ shRNA was analyzed by reverse transcriptase PCR for PTPμ, PTPρ, PTPκ, and PCP-2 (PTPλ) expression. PTPμ mRNA levels were decreased by shRNA targeting PTPμ. PTPκ and PCP-2 mRNA levels were unaffected; PTPρ was not expressed. The numbers on the left side of the gel represent the PCR product size in base pairs.
Figure Legend Snippet: Protein tyrosine phosphatase μ (PTPμ) mRNA and protein levels are decreased by short-hairpin RNA (shRNA). (A) Lysates from U-87 MG cells infected with control or PTPμ shRNA lentivirus were immunoblotted for PTPμ using the intracellular antibody SK-18. PTPμ shRNA reduced PTPμ protein levels in comparison to uninfected and control shRNA-infected cells. Vinculin was used as a loading control. Densitometry values for PTPμ and vinculin are shown under each lane and were normalized to parental cells. The numbers on the left side of the immunoblot correspond to the molecular weight in kilodaltons. (B) RNA from U-87 MG cells expressing control or PTPμ shRNA was analyzed by reverse transcriptase PCR for PTPμ, PTPρ, PTPκ, and PCP-2 (PTPλ) expression. PTPμ mRNA levels were decreased by shRNA targeting PTPμ. PTPκ and PCP-2 mRNA levels were unaffected; PTPρ was not expressed. The numbers on the left side of the gel represent the PCR product size in base pairs.

Techniques Used: shRNA, Infection, Molecular Weight, Expressing, Polymerase Chain Reaction

2) Product Images from "Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades"

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades

Journal: Molecular and Cellular Biology

doi: 10.1128/MCB.25.9.3670-3681.2005

Effect of MLK3 deficiency on AP-1 and NF-κB. (A) The expression of several genes that encode proteins that can form AP-1 transcription factors by exponentially growing WT and Mlk3 −/− MEF was examined by a RNase protection assay to detect mRNA. The expression of ribosomal protein L32 mRNA was examined in control experiments. (B) WT and Mlk3 −/− MEF were incubated with TNF (10 ng/ml), and the degradation of IκBα was examined by immunoblot analysis. Control experiments were performed by probing immunoblots with an antibody to α-tubulin. (C) The expression of TNF-R1 and TNF-R2 was examined by an RNase protection assay to measure the amount of TNF receptor mRNA in WT and Mlk3 −/− MEF. Control experiments were performed by measuring the amount of ribosomal protein L32 mRNA. The data were quantitated by phosphorimager analysis, and the relative TNF receptor expression was calculated as the ratio of TNF receptor mRNA to L32 mRNA. (D) WT and Mlk3 −/− MEF were incubated with TNF (10 ng/ml) for 24 h, and the amount of IL-6 and L32 mRNA was examined by an RNase protection assay. The data were quantitated by phosphorimager analysis, and the relative IL-6 mRNA expression was calculated as the ratio of IL-6 mRNA to L32 mRNA.
Figure Legend Snippet: Effect of MLK3 deficiency on AP-1 and NF-κB. (A) The expression of several genes that encode proteins that can form AP-1 transcription factors by exponentially growing WT and Mlk3 −/− MEF was examined by a RNase protection assay to detect mRNA. The expression of ribosomal protein L32 mRNA was examined in control experiments. (B) WT and Mlk3 −/− MEF were incubated with TNF (10 ng/ml), and the degradation of IκBα was examined by immunoblot analysis. Control experiments were performed by probing immunoblots with an antibody to α-tubulin. (C) The expression of TNF-R1 and TNF-R2 was examined by an RNase protection assay to measure the amount of TNF receptor mRNA in WT and Mlk3 −/− MEF. Control experiments were performed by measuring the amount of ribosomal protein L32 mRNA. The data were quantitated by phosphorimager analysis, and the relative TNF receptor expression was calculated as the ratio of TNF receptor mRNA to L32 mRNA. (D) WT and Mlk3 −/− MEF were incubated with TNF (10 ng/ml) for 24 h, and the amount of IL-6 and L32 mRNA was examined by an RNase protection assay. The data were quantitated by phosphorimager analysis, and the relative IL-6 mRNA expression was calculated as the ratio of IL-6 mRNA to L32 mRNA.

Techniques Used: Expressing, Rnase Protection Assay, Incubation, Western Blot

Related Articles

Blocking Assay:

Article Title: Understanding cooperativity of microRNAs via microRNA association networks
Article Snippet: It consists of triplicated mRNA (Affymetrix Mouse Gene 1.0 ST Array) and duplicated miRNA (Ambion mirVana™ miRNA Bioarray V2) expression profiles from 7 time points, resulting 21 and 14 hybridizations for mRNA and miRNA, respectively. .. N2a mouse neuroblastoma cells were treated with Actinomycin D to block transcription at 0, 1, 2, 4, 8, 12 and 24 h time points.

Real-time Polymerase Chain Reaction:

Article Title: HOXA9 is a novel myopia risk gene
Article Snippet: Paragraph title: Real-time PCR, western blot ... We used 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers to create cDNA.

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers. .. Quantitative real‐time PCR (qPCR) was performed with an ABI PRISM 7900 sequence detector and SYBR green reagents (Applied Biosystems, Waltham, MA, USA).

Article Title: miR-155 and miR-122 Expression of Spermatozoa in Obese Subjects
Article Snippet: The StepOne Plus (Applied Biosystems) was used for real-time PCR using TaqMan® Universal Master Mix II, and does not contain Uracil N -glycosylase (UNG) (Applied Biosystems). .. For mRNA expression, 1 μg RNA was transcribed into cDNA using a high-capacity cDNA reverse transcription kit for mRNA (Applied Biosystems).

Article Title: Metabolic reprogramming of metastatic breast cancer and melanoma by let-7a microRNA
Article Snippet: .. Quantitative real-time PCR Isolation of total RNA and cDNA synthesis were performed using Cell-to-Ct kit for mRNA (Ambion), following the manufacturers protocol. .. Reaction mix for quantitative polymerase chain reaction (qPCR) was prepared using cDNA from 100 to 500 cells/well and TaqMan gene expression master mix (Ambion).

Article Title: Anti‐inflammatory effects of miR‐21 in the macrophage response to peritonitis
Article Snippet: For mRNA expression, RNA was transcribed into cDNA using high‐capacity cDNA reverse transcription kit for mRNA (Applied Biosystems). .. The StepOne Plus (Applied Biosystems) was used to run the real‐time PCR using fast advanced master mix and the fast protocol (all Life Technologies).

Microarray:

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades
Article Snippet: .. To test this hypothesis, we examined MAP3K gene expression in wild-type and Mlk3 −/− MEF by microarray analysis of isolated mRNA (Affymetrix). .. This analysis did not demonstrate differences in MAP3K gene expression (other than Mlk3 ) between wild-type and Mlk3 −/− MEF (data not shown).

Article Title: Reprogramming of COPD lung fibroblasts through formation of induced pluripotent stem cells
Article Snippet: .. Hybridizations and microarray analysis for mRNA (Affymetrix Human Gene 1.0 ST Array) and miRNA (Affymetrix miRNAs Array), following the manufacturer's recommendations, were performed in the University of Nebraska Medical Center microarray core facility. .. Analysis of Affymetrix data were conducted with BRB Array Tools version 3.8.0 stable release, developed by Dr. Richard Simon ( ) and the R programming language with Bioconductor project ( ).

Incubation:

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70
Article Snippet: In order to quantify viral transcription in early infection, an oligo(dT) primer was used to reverse transcribe only mRNA (Life Technologies). .. Infectious virus released into the supernatant was quantified using a standard immune-plaque assay in which rhesus monkey kidney epithelial cells (LLC-MK2 cells) were exposed to supernatants for 2 h, then incubated under methyl cellulose for 7 days to allow plaque formation prior to fixation and immune-detection of hMPV-positive plaques [ ].

Expressing:

Article Title: Big data and computational biology strategy for personalized prognosis
Article Snippet: TCGA expression data comprised of 463 primary solid ovarian cancer tissue samples from 11 batches (containing between 21 to 47 samples in each batch). .. Pre-processing of the mRNA (Affymetrix U133A) data were performed via quality assessment within each batch, background correction, normalization and batch effect adjustment as previously described [ ].

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: Total RNA and protein extracted from the gastrocnemius muscle or C2C12 cells were used to analyse the expression levels of VEGF‐A, VEGF‐A164a , VEGF‐A165b , VEGFR‐2, Hypoxia‐inducible factor 3‐alpha (HIF‐3α), Tumour Protein 53 (TP53), Cyclin D‐dependent kinases 6 (CDK6) and splicing factor SC35. .. Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers.

Article Title: miR-155 and miR-122 Expression of Spermatozoa in Obese Subjects
Article Snippet: .. For mRNA expression, 1 μg RNA was transcribed into cDNA using a high-capacity cDNA reverse transcription kit for mRNA (Applied Biosystems). ..

Article Title: Understanding cooperativity of microRNAs via microRNA association networks
Article Snippet: .. Radiation dataset [ ] consists of triplicated mRNA (Affymetrix Human Gene 1.0 ST Array) and duplicated miRNA (Ambion mirVana™ miRNA Bioarray V2) expression profiles from 6 time points, resulting 18 and 12 hybridizations for mRNA and miRNA, respectively. .. Two lung cancer cell lines, H460 and H1299 were irradiated at 2 Gy, and harvested after 0, 2, 4, 8, 12 and 24 hours to examine the expressions.

Article Title: Metabolic reprogramming of metastatic breast cancer and melanoma by let-7a microRNA
Article Snippet: Quantitative real-time PCR Isolation of total RNA and cDNA synthesis were performed using Cell-to-Ct kit for mRNA (Ambion), following the manufacturers protocol. .. Reaction mix for quantitative polymerase chain reaction (qPCR) was prepared using cDNA from 100 to 500 cells/well and TaqMan gene expression master mix (Ambion).

Article Title: mRNA stability in the nucleus
Article Snippet: However, all of them have similar distribution in the genome, either an expression peak around 50 nt downstream of transcription start site (TSS) in the same orientation as the gene expression or 250 nt upstream of TSS in a divergent transcription (Kapranov et al., ; Core et al., ; Seila et al., ; Jacquier, ). .. They are short-lived RNA molecules, and their 5′ ends are capped with a different mechanism, unlike normal mRNA (Affymetrix/Cold Spring Harbor Laboratory ENCODE Transcriptome Project, ).

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades
Article Snippet: .. To test this hypothesis, we examined MAP3K gene expression in wild-type and Mlk3 −/− MEF by microarray analysis of isolated mRNA (Affymetrix). .. This analysis did not demonstrate differences in MAP3K gene expression (other than Mlk3 ) between wild-type and Mlk3 −/− MEF (data not shown).

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70
Article Snippet: ) and standard curves were generated using 10-fold serial dilutions of expression plasmids encoding the nucleocapsid (N) gene of RSV or hMPV (Biomatik). .. In order to quantify viral transcription in early infection, an oligo(dT) primer was used to reverse transcribe only mRNA (Life Technologies).

Article Title: Anti‐inflammatory effects of miR‐21 in the macrophage response to peritonitis
Article Snippet: .. For mRNA expression, RNA was transcribed into cDNA using high‐capacity cDNA reverse transcription kit for mRNA (Applied Biosystems). ..

Article Title: A combinatorial radiographic phenotype may stratify patient survival and be associated with invasion and proliferation characteristics in glioblastoma
Article Snippet: .. Level 3 expression data for both mRNA (Affymetrix U133Av2 BI Platform: Affymetrix HT HG U133A) and miRNA (UNC Agilent Human miRNA 8 × 15 K) were downloaded from the TCGA data portal. .. These data are normalized and were used for differential expression analysis.

Article Title: Understanding cooperativity of microRNAs via microRNA association networks
Article Snippet: .. It consists of triplicated mRNA (Affymetrix Mouse Gene 1.0 ST Array) and duplicated miRNA (Ambion mirVana™ miRNA Bioarray V2) expression profiles from 7 time points, resulting 21 and 14 hybridizations for mRNA and miRNA, respectively. .. N2a mouse neuroblastoma cells were treated with Actinomycin D to block transcription at 0, 1, 2, 4, 8, 12 and 24 h time points.

Article Title: Reprogramming of COPD lung fibroblasts through formation of induced pluripotent stem cells
Article Snippet: Hybridizations and microarray analysis for mRNA (Affymetrix Human Gene 1.0 ST Array) and miRNA (Affymetrix miRNAs Array), following the manufacturer's recommendations, were performed in the University of Nebraska Medical Center microarray core facility. .. Low-level analysis, which converts probe level data to a gene level expression data as well as normalization, was done using RMA.

Western Blot:

Article Title: HOXA9 is a novel myopia risk gene
Article Snippet: Paragraph title: Real-time PCR, western blot ... We used 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers to create cDNA.

Transfection:

Article Title: PTP? suppresses glioma cell migration and dispersal
Article Snippet: Lentiviral shRNA constructs V2LHS_171008 (shRNA 1) and V2LHS_171013 (shRNA 2) targeting human PTPμ mRNA were purchased from Open Biosystems (Huntsville, AL). .. Vesicular stomatitis virus glycoprotein–pseudotyped lentiviral particles were generated by triple transfection of shRNA constructs with the packaging plasmids pCMVΔR8.91 and pMD.G into 293T cells using Lipofectamine 2000 (Invitrogen) according to a previously described protocol.

Infection:

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70
Article Snippet: .. In order to quantify viral transcription in early infection, an oligo(dT) primer was used to reverse transcribe only mRNA (Life Technologies). ..

Generated:

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70
Article Snippet: ) and standard curves were generated using 10-fold serial dilutions of expression plasmids encoding the nucleocapsid (N) gene of RSV or hMPV (Biomatik). .. In order to quantify viral transcription in early infection, an oligo(dT) primer was used to reverse transcribe only mRNA (Life Technologies).

Article Title: Anti‐inflammatory effects of miR‐21 in the macrophage response to peritonitis
Article Snippet: For mRNA expression, RNA was transcribed into cDNA using high‐capacity cDNA reverse transcription kit for mRNA (Applied Biosystems). .. StepOne Plus software v2.1 (Applied Biosystems) generated cycle threshold values, which were used to calculate the fold changes using the ΔΔ cycle threshold method [ ].

Article Title: PTP? suppresses glioma cell migration and dispersal
Article Snippet: Lentiviral shRNA constructs V2LHS_171008 (shRNA 1) and V2LHS_171013 (shRNA 2) targeting human PTPμ mRNA were purchased from Open Biosystems (Huntsville, AL). .. Vesicular stomatitis virus glycoprotein–pseudotyped lentiviral particles were generated by triple transfection of shRNA constructs with the packaging plasmids pCMVΔR8.91 and pMD.G into 293T cells using Lipofectamine 2000 (Invitrogen) according to a previously described protocol.

Polymerase Chain Reaction:

Article Title: HOXA9 is a novel myopia risk gene
Article Snippet: We used 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers to create cDNA. .. The sequences of PCR primers are shown in Table .

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers. .. The sequences of PCR primers are shown in Table , and primer sequences for VEGF‐A164a and VEGF‐A165b were from Kikuchi et al .

Article Title: MicroRNA let-7g inhibits angiotensin II-induced endothelial senescence via the LOX-1-independent mechanism
Article Snippet: Total RNA was extracted from the HUVECs using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and complementary (c)DNA was produced using 1 μ g starting mRNA (Applied Biosystems; Thermo Fisher Scientific, Inc.) and random hexamers. .. The sequences of the PCR primers are listed in .

Quantitative RT-PCR:

Article Title: MicroRNA let-7g inhibits angiotensin II-induced endothelial senescence via the LOX-1-independent mechanism
Article Snippet: Paragraph title: RNA isolation and RT-qPCR ... Total RNA was extracted from the HUVECs using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and complementary (c)DNA was produced using 1 μ g starting mRNA (Applied Biosystems; Thermo Fisher Scientific, Inc.) and random hexamers.

Immunofluorescence:

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades
Article Snippet: Indeed, immunofluorescence microscopy with a monoclonal antibody to α-tubulin indicated the presence of similar microtubule networks in wild-type and Mlk3 −/− MEF (Fig. ). .. To test this hypothesis, we examined MAP3K gene expression in wild-type and Mlk3 −/− MEF by microarray analysis of isolated mRNA (Affymetrix).

Irradiation:

Article Title: Understanding cooperativity of microRNAs via microRNA association networks
Article Snippet: Radiation dataset [ ] consists of triplicated mRNA (Affymetrix Human Gene 1.0 ST Array) and duplicated miRNA (Ambion mirVana™ miRNA Bioarray V2) expression profiles from 6 time points, resulting 18 and 12 hybridizations for mRNA and miRNA, respectively. .. Two lung cancer cell lines, H460 and H1299 were irradiated at 2 Gy, and harvested after 0, 2, 4, 8, 12 and 24 hours to examine the expressions.

Isolation:

Article Title: Metabolic reprogramming of metastatic breast cancer and melanoma by let-7a microRNA
Article Snippet: .. Quantitative real-time PCR Isolation of total RNA and cDNA synthesis were performed using Cell-to-Ct kit for mRNA (Ambion), following the manufacturers protocol. .. Reaction mix for quantitative polymerase chain reaction (qPCR) was prepared using cDNA from 100 to 500 cells/well and TaqMan gene expression master mix (Ambion).

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades
Article Snippet: .. To test this hypothesis, we examined MAP3K gene expression in wild-type and Mlk3 −/− MEF by microarray analysis of isolated mRNA (Affymetrix). .. This analysis did not demonstrate differences in MAP3K gene expression (other than Mlk3 ) between wild-type and Mlk3 −/− MEF (data not shown).

Article Title: Anti‐inflammatory effects of miR‐21 in the macrophage response to peritonitis
Article Snippet: Paragraph title: RNA isolation and miRNA and mRNA expression ... For mRNA expression, RNA was transcribed into cDNA using high‐capacity cDNA reverse transcription kit for mRNA (Applied Biosystems).

Article Title: Reprogramming of COPD lung fibroblasts through formation of induced pluripotent stem cells
Article Snippet: mRNA and microRNAs (miRNAs) were extracted using mirVana miRNA Isolation Kit (Ambion, Applied Biosystems, Austin, TX) following the manufacturer's instructions. .. Hybridizations and microarray analysis for mRNA (Affymetrix Human Gene 1.0 ST Array) and miRNA (Affymetrix miRNAs Array), following the manufacturer's recommendations, were performed in the University of Nebraska Medical Center microarray core facility.

Article Title: MicroRNA let-7g inhibits angiotensin II-induced endothelial senescence via the LOX-1-independent mechanism
Article Snippet: Paragraph title: RNA isolation and RT-qPCR ... Total RNA was extracted from the HUVECs using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and complementary (c)DNA was produced using 1 μ g starting mRNA (Applied Biosystems; Thermo Fisher Scientific, Inc.) and random hexamers.

Microscopy:

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades
Article Snippet: Indeed, immunofluorescence microscopy with a monoclonal antibody to α-tubulin indicated the presence of similar microtubule networks in wild-type and Mlk3 −/− MEF (Fig. ). .. To test this hypothesis, we examined MAP3K gene expression in wild-type and Mlk3 −/− MEF by microarray analysis of isolated mRNA (Affymetrix).

Mouse Assay:

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: mRNA and protein analysis The gastrocnemius muscles of ischaemic and non‐ischaemic limbs were harvested on day 21 after mice were killed. .. Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers.

Sequencing:

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers. .. Quantitative real‐time PCR (qPCR) was performed with an ABI PRISM 7900 sequence detector and SYBR green reagents (Applied Biosystems, Waltham, MA, USA).

Construct:

Article Title: PTP? suppresses glioma cell migration and dispersal
Article Snippet: .. Lentiviral shRNA constructs V2LHS_171008 (shRNA 1) and V2LHS_171013 (shRNA 2) targeting human PTPμ mRNA were purchased from Open Biosystems (Huntsville, AL). .. A control lentiviral shRNA construct was a gift from Drs. E. Johnson and R. Keri.

Plasmid Preparation:

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70
Article Snippet: A plasmid encoding the β-actin gene (Biomatik) was used as a housekeeping standard. .. In order to quantify viral transcription in early infection, an oligo(dT) primer was used to reverse transcribe only mRNA (Life Technologies).

Software:

Article Title: Anti‐inflammatory effects of miR‐21 in the macrophage response to peritonitis
Article Snippet: For mRNA expression, RNA was transcribed into cDNA using high‐capacity cDNA reverse transcription kit for mRNA (Applied Biosystems). .. StepOne Plus software v2.1 (Applied Biosystems) generated cycle threshold values, which were used to calculate the fold changes using the ΔΔ cycle threshold method [ ].

SYBR Green Assay:

Article Title: HOXA9 is a novel myopia risk gene
Article Snippet: We used 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers to create cDNA. .. Real-time PCR was performed on an ABI StepOnePlus™ Real-Time PCR Systems (Applied Biosystems) in duplicate using 5 μl 2× SYBR Green qPCR Master Mix, 0.2 μl primer sets, 1 μl cDNA and 3.6 μl nucleotide-free H2O, which yielded a 10 μl reaction.

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers. .. Quantitative real‐time PCR (qPCR) was performed with an ABI PRISM 7900 sequence detector and SYBR green reagents (Applied Biosystems, Waltham, MA, USA).

shRNA:

Article Title: PTP? suppresses glioma cell migration and dispersal
Article Snippet: .. Lentiviral shRNA constructs V2LHS_171008 (shRNA 1) and V2LHS_171013 (shRNA 2) targeting human PTPμ mRNA were purchased from Open Biosystems (Huntsville, AL). .. A control lentiviral shRNA construct was a gift from Drs. E. Johnson and R. Keri.

Produced:

Article Title: Micro RNA let‐7g possesses a therapeutic potential for peripheral artery disease
Article Snippet: .. Total RNA was extracted with TRIzol® Reagent (Invitrogen), and cDNA was produced using 1 μg of starting mRNA (Applied Biosystems, Darmstadt, Germany) and random hexamers. .. The sequences of PCR primers are shown in Table , and primer sequences for VEGF‐A164a and VEGF‐A165b were from Kikuchi et al .

Article Title: MicroRNA let-7g inhibits angiotensin II-induced endothelial senescence via the LOX-1-independent mechanism
Article Snippet: .. Total RNA was extracted from the HUVECs using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and complementary (c)DNA was produced using 1 μ g starting mRNA (Applied Biosystems; Thermo Fisher Scientific, Inc.) and random hexamers. ..

Migration:

Article Title: Role of MLK3 in the Regulation of Mitogen-Activated Protein Kinase Signaling Cascades
Article Snippet: The defects in cell proliferation and migration predicted by RNAi-based studies ( , ) were not observed in Mlk3 −/− MEF (Fig. ). .. To test this hypothesis, we examined MAP3K gene expression in wild-type and Mlk3 −/− MEF by microarray analysis of isolated mRNA (Affymetrix).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher mirvana mrna isolation kit
    Mirvana Mrna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana mrna isolation kit/product/Thermo Fisher
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    mirvana mrna isolation kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher foxl2 mrna
    Foxl2 Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/foxl2 mrna/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    foxl2 mrna - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    N/A
    Thermo Scientific MagJet Wash Buffer is a component of the MagJET mRNA Enrichment Kit K2811 2812 and may be purchased separately Provided in 220 mL aliquots Related ProductsMagJET mRNA Enrichment
      Buy from Supplier

    Image Search Results