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Illumina Inc mrna poly a tail
Mrna Poly A Tail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mrna poly a tail/product/Illumina Inc
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mrna poly a tail - by Bioz Stars, 2020-09
91/100 stars

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Selection:

Article Title: High-Resolution Global Analysis of the Influences of Bas1 and Ino4 Transcription Factors on Meiotic DNA Break Distributions in Saccharomyces cerevisiae
Article Snippet: .. Messenger RNA (mRNA) was enriched by poly-A selection and sequenced using Illumina HiSeq in the Genomics Resources Core Facility of Weill Cornell Medical College. .. Reads were mapped to the S. cerevisiae sacCer2 genome assembly using the Spliced Transcripts Alignment to a Reference (STAR) software ( ).

Article Title: Robust Innate Immunity of Young Rabbits Mediates Resistance to Rabbit Hemorrhagic Disease Caused by Lagovirus Europaeus GI.1 But Not GI.2
Article Snippet: .. Messenger RNA (mRNA) was enriched in the total RNA samples using polyA selection and sequenced using two 75 bp single-end NextSeq lanes (Illumina, Scoresby, VIC, Australia) at the Australian Cancer Research Foundation (ACRF) Biomolecular Resource Facility (The John Curtin School of Medical Research, Australian National University, Canberra, Australia). .. RNA-Seq reads were cleaned by removing Illumina TruSeq adapters, trimming sequences when the quality score dropped below 20, and discarding reads shorter than 50 bp using Trimmomatic v.0.32 [ ].

Sample Prep:

Article Title: Transcriptome analysis of the white pine blister rust pathogen Cronartium ribicola: de novo assembly, expression profiling, and identification of candidate effectors
Article Snippet: .. Messenger RNA (mRNA) was separated using an RNA-seq sample preparation kit (Illumina). cDNA libraries were constructed with specific 6-bp nucleotide bar-coding tags. .. Tagged cDNA libraries were pooled in equal ratios and used for 100-bp PE sequencing on the Illumina HiSeq2000 instrument (Illumina, San Diego, CA, USA) at the National Research Council of Canada (Saskatoon, Canada).

Isolation:

Article Title: Multi-Tissue Transcriptome Profiling of North American Derived Atlantic Salmon
Article Snippet: .. Messenger RNA (mRNA) was isolated from pituitary gland, brain, ovary, and liver before Illumina sequencing produced a total of 640 million 150-bp paired-end reads. ..

Article Title: Primate innate immune responses to bacterial and viral pathogens reveals an evolutionary trade-off between strength and specificity
Article Snippet: .. Messenger RNA (mRNA) was isolated by magnetic bead and converted into RNA libraries using the Illumina TruSeq RNA Library preparation kit v2 according to the manufacturer’s instructions (Illumina, San Diego, CA, USA). ..

Construct:

Article Title: Transcriptome analysis of the white pine blister rust pathogen Cronartium ribicola: de novo assembly, expression profiling, and identification of candidate effectors
Article Snippet: .. Messenger RNA (mRNA) was separated using an RNA-seq sample preparation kit (Illumina). cDNA libraries were constructed with specific 6-bp nucleotide bar-coding tags. .. Tagged cDNA libraries were pooled in equal ratios and used for 100-bp PE sequencing on the Illumina HiSeq2000 instrument (Illumina, San Diego, CA, USA) at the National Research Council of Canada (Saskatoon, Canada).

Produced:

Article Title: Multi-Tissue Transcriptome Profiling of North American Derived Atlantic Salmon
Article Snippet: .. Messenger RNA (mRNA) was isolated from pituitary gland, brain, ovary, and liver before Illumina sequencing produced a total of 640 million 150-bp paired-end reads. ..

Sequencing:

Article Title: Multi-Tissue Transcriptome Profiling of North American Derived Atlantic Salmon
Article Snippet: .. Messenger RNA (mRNA) was isolated from pituitary gland, brain, ovary, and liver before Illumina sequencing produced a total of 640 million 150-bp paired-end reads. ..

Article Title: Loss of prion protein induces a primed state of type I interferon-responsive genes
Article Snippet: .. Individual RNA samples of high quality (RIN ≥ 9.8) were sequenced by mRNA poly-A-tail, paired-end sequencing (Illumina HiSeq 2000) with 91 bp read-lengths (Beijing Genomics Institute (BGI), Hong Kong), retrieving a minimum depth of 5G clean data per sample. .. In detail, after the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) were used to isolate mRNA.

RNA Sequencing Assay:

Article Title: Transcriptome analysis of the white pine blister rust pathogen Cronartium ribicola: de novo assembly, expression profiling, and identification of candidate effectors
Article Snippet: .. Messenger RNA (mRNA) was separated using an RNA-seq sample preparation kit (Illumina). cDNA libraries were constructed with specific 6-bp nucleotide bar-coding tags. .. Tagged cDNA libraries were pooled in equal ratios and used for 100-bp PE sequencing on the Illumina HiSeq2000 instrument (Illumina, San Diego, CA, USA) at the National Research Council of Canada (Saskatoon, Canada).

Article Title: The Causal Role of IL-4 and IL-13 in Schistosoma mansoni Pulmonary Hypertension
Article Snippet: .. Messenger RNA (mRNA) was quantified using a HiSeq 2000 RNA sequencing (RNA-seq) system (Illumina, San Diego, CA), as previously described; the data are deposited in National Center for Biotechnology Information’s GEO dataset ( ). .. Immunohistochemistry staining was performed on formalin-fixed paraffin-embedded samples using the reagents in Table E4 on three types of lung tissue: 1.

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    Illumina Inc mirna mrna negative regulation pattern
    The expression patterns of differentially expressed <t>miRNAs</t> and their predicted differentially expressed <t>mRNA</t> targets were analyzed by quantitative real-time PCR (qPCR) in wandering stage (WS), late wandering stage (LWS, the body contraction just before pupariation) and white puparium stage (WPS) of Bactrocera dorsalis . The results of Illumina sequencing also showed here. They included (A) relative expression of five miRNAs in Illumina sequencing, (B) relative expression of eight targets in Illumina sequencing, (C) relative expression of five miRNAs in qPCR, (D) relative expression of eight targets in qPCR. The qPCR and Illumina sequencing results were analyzed with one-way analysis of variance (ANOVA), and the means were separated with Tukey’s HSD post hoc test ( P
    Mirna Mrna Negative Regulation Pattern, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirna mrna negative regulation pattern/product/Illumina Inc
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mirna mrna negative regulation pattern - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    Illumina Inc mrna
    Transcriptional analysis of hrtAB expression during heme stress. Samples were taken from LO28 wild-type, Δ hssR and Δ hrtA cultures exposed to 4 or 8 μM hemin stress for 30 and 60 min, as well as from non-stressed cultures (0 μM). The northern blot was probed for hrtA <t>mRNA,</t> hrtB mRNA and 16S <t>rRNA</t> (loading control). Levels of hrtA mRNA and hrtB mRNA (normalized to 16S) relative to the ‘0 μM, 30 min’ sample of each strain are shown below each lane.
    Mrna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrna/product/Illumina Inc
    Average 94 stars, based on 329 article reviews
    Price from $9.99 to $1999.99
    mrna - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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    The expression patterns of differentially expressed miRNAs and their predicted differentially expressed mRNA targets were analyzed by quantitative real-time PCR (qPCR) in wandering stage (WS), late wandering stage (LWS, the body contraction just before pupariation) and white puparium stage (WPS) of Bactrocera dorsalis . The results of Illumina sequencing also showed here. They included (A) relative expression of five miRNAs in Illumina sequencing, (B) relative expression of eight targets in Illumina sequencing, (C) relative expression of five miRNAs in qPCR, (D) relative expression of eight targets in qPCR. The qPCR and Illumina sequencing results were analyzed with one-way analysis of variance (ANOVA), and the means were separated with Tukey’s HSD post hoc test ( P

    Journal: Frontiers in Physiology

    Article Title: Genome-Wide Analysis of MicroRNAs in Relation to Pupariation in Oriental Fruit Fly

    doi: 10.3389/fphys.2019.00301

    Figure Lengend Snippet: The expression patterns of differentially expressed miRNAs and their predicted differentially expressed mRNA targets were analyzed by quantitative real-time PCR (qPCR) in wandering stage (WS), late wandering stage (LWS, the body contraction just before pupariation) and white puparium stage (WPS) of Bactrocera dorsalis . The results of Illumina sequencing also showed here. They included (A) relative expression of five miRNAs in Illumina sequencing, (B) relative expression of eight targets in Illumina sequencing, (C) relative expression of five miRNAs in qPCR, (D) relative expression of eight targets in qPCR. The qPCR and Illumina sequencing results were analyzed with one-way analysis of variance (ANOVA), and the means were separated with Tukey’s HSD post hoc test ( P

    Article Snippet: TABLE S9 List of miRNAs and predicted target mRNAs coinciding with the miRNA–mRNA negative regulation pattern in WS vs. LWS of Bactrocera dorsalis .

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Sequencing

    Transcriptional analysis of hrtAB expression during heme stress. Samples were taken from LO28 wild-type, Δ hssR and Δ hrtA cultures exposed to 4 or 8 μM hemin stress for 30 and 60 min, as well as from non-stressed cultures (0 μM). The northern blot was probed for hrtA mRNA, hrtB mRNA and 16S rRNA (loading control). Levels of hrtA mRNA and hrtB mRNA (normalized to 16S) relative to the ‘0 μM, 30 min’ sample of each strain are shown below each lane.

    Journal: Frontiers in Microbiology

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein

    doi: 10.3389/fmicb.2018.03090

    Figure Lengend Snippet: Transcriptional analysis of hrtAB expression during heme stress. Samples were taken from LO28 wild-type, Δ hssR and Δ hrtA cultures exposed to 4 or 8 μM hemin stress for 30 and 60 min, as well as from non-stressed cultures (0 μM). The northern blot was probed for hrtA mRNA, hrtB mRNA and 16S rRNA (loading control). Levels of hrtA mRNA and hrtB mRNA (normalized to 16S) relative to the ‘0 μM, 30 min’ sample of each strain are shown below each lane.

    Article Snippet: To enrich mRNA and remove ribosomal RNA (rRNA) from total RNA, total RNA was treated with Ribo-Zero rRNA removal kit (Illumina).

    Techniques: Expressing, Northern Blot

    Transcriptional analysis of lmo1634 expression during heme stress. Samples were taken from wild-type EGD-e, wild-type EGD and mutant EGDΔ resD cultures exposed to 4 or 8 μM hemin stress for 30 and 60 min, as well as from non-stressed cultures (0 μM). Northern blots were probed for lmo1634 mRNA, hrtA mRNA and 16S rRNA (loading control). Levels of lmo1634 mRNA and hrtA mRNA (normalized to 16S) relative to the ‘0 μM, 30 min’ sample of each strain are shown below each lane.

    Journal: Frontiers in Microbiology

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein

    doi: 10.3389/fmicb.2018.03090

    Figure Lengend Snippet: Transcriptional analysis of lmo1634 expression during heme stress. Samples were taken from wild-type EGD-e, wild-type EGD and mutant EGDΔ resD cultures exposed to 4 or 8 μM hemin stress for 30 and 60 min, as well as from non-stressed cultures (0 μM). Northern blots were probed for lmo1634 mRNA, hrtA mRNA and 16S rRNA (loading control). Levels of lmo1634 mRNA and hrtA mRNA (normalized to 16S) relative to the ‘0 μM, 30 min’ sample of each strain are shown below each lane.

    Article Snippet: To enrich mRNA and remove ribosomal RNA (rRNA) from total RNA, total RNA was treated with Ribo-Zero rRNA removal kit (Illumina).

    Techniques: Expressing, Mutagenesis, Northern Blot

    Selected DElncRNA-DEmRNA co-expression network. Ovals and rhombuses represent DEmRNAs and DElncRNAs, respectively, comparing AS patients and normal controls. Shapes with bold black outlines indicate the top 10 DEmRNAs/DElncRNAs when comparing AS and normal controls. AS, ankylosing spondylitis; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA.

    Journal: International Journal of Molecular Medicine

    Article Title: Identification of the key genes and long non-coding RNAs in ankylosing spondylitis using RNA sequencing

    doi: 10.3892/ijmm.2018.4038

    Figure Lengend Snippet: Selected DElncRNA-DEmRNA co-expression network. Ovals and rhombuses represent DEmRNAs and DElncRNAs, respectively, comparing AS patients and normal controls. Shapes with bold black outlines indicate the top 10 DEmRNAs/DElncRNAs when comparing AS and normal controls. AS, ankylosing spondylitis; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA.

    Article Snippet: Validation of the expression of DEmRNAs and DElncRNAs using the GSE73754 and GSE25101 datasets The mRNA expression profile of 52 patients with AS and 20 normal controls (Canadian cohort) in the GSE73754 dataset (GPL10558 Illumina HumanHT-12 V4.0 expression beadchip), which was downloaded from the Gene Expression Omnibus (GEO; www.ncbi.nlm.nih.gov/gds ).

    Techniques: Expressing

    AS-specific PPI network. Blue and red shapes represent proteins encoded by down- and upregulated DEmRNAs, respectively, when comparing patients with AS and normal controls. Among these, blue shapes with green outlines represent proteins encoded by the top 10 downregulated DEmRNAs; red shapes with red outlines represent proteins encoded by the top 10 upregulated DEmRNAs. AS, ankylosing spondylitis; DEmRNA, differentially expressed mRNA.

    Journal: International Journal of Molecular Medicine

    Article Title: Identification of the key genes and long non-coding RNAs in ankylosing spondylitis using RNA sequencing

    doi: 10.3892/ijmm.2018.4038

    Figure Lengend Snippet: AS-specific PPI network. Blue and red shapes represent proteins encoded by down- and upregulated DEmRNAs, respectively, when comparing patients with AS and normal controls. Among these, blue shapes with green outlines represent proteins encoded by the top 10 downregulated DEmRNAs; red shapes with red outlines represent proteins encoded by the top 10 upregulated DEmRNAs. AS, ankylosing spondylitis; DEmRNA, differentially expressed mRNA.

    Article Snippet: Validation of the expression of DEmRNAs and DElncRNAs using the GSE73754 and GSE25101 datasets The mRNA expression profile of 52 patients with AS and 20 normal controls (Canadian cohort) in the GSE73754 dataset (GPL10558 Illumina HumanHT-12 V4.0 expression beadchip), which was downloaded from the Gene Expression Omnibus (GEO; www.ncbi.nlm.nih.gov/gds ).

    Techniques:

    Top 15 most significantly enriched GO terms of DEmRNAs co-expressed with DElncRNAs. The x-axis presents the number of DEmRNAs enriched in the GO terms and the y-axis presents the GO terms. (A) Biological process; (B) Cellular component; (C) Molecular function. GO, Gene Ontology; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA; FDR, false discovery rate.

    Journal: International Journal of Molecular Medicine

    Article Title: Identification of the key genes and long non-coding RNAs in ankylosing spondylitis using RNA sequencing

    doi: 10.3892/ijmm.2018.4038

    Figure Lengend Snippet: Top 15 most significantly enriched GO terms of DEmRNAs co-expressed with DElncRNAs. The x-axis presents the number of DEmRNAs enriched in the GO terms and the y-axis presents the GO terms. (A) Biological process; (B) Cellular component; (C) Molecular function. GO, Gene Ontology; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA; FDR, false discovery rate.

    Article Snippet: Validation of the expression of DEmRNAs and DElncRNAs using the GSE73754 and GSE25101 datasets The mRNA expression profile of 52 patients with AS and 20 normal controls (Canadian cohort) in the GSE73754 dataset (GPL10558 Illumina HumanHT-12 V4.0 expression beadchip), which was downloaded from the Gene Expression Omnibus (GEO; www.ncbi.nlm.nih.gov/gds ).

    Techniques:

    Validation of the expression of selected DEmRNAs and DElncRNAs between patients with AS and the normal controls in the GSE25101 dataset. The x-axis presents the AS and normal control groups and the y-axis presents the expression levels. (A) The selected DEmRNAs and (B) The selected DElncRNAs. AS, ankylosing spondylitis; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA; CARD11, caspase recruitment domain-containing protein 11; DNMT1, DNA methyltransferase 1; PDCD1, programmed cell death 1; PLCG1, phospholipase Cγ1; MANSC1, MANSC domain containing 1; ATP2A2, adenosine triphosphatase sarcoplasmic/endoplasmic reticulum Ca 2+ transporting 2; MZF1, myeloid zinc finger 1; CECR7, cat eye syndrome chromosome region candidate 7; HYMAI, hydatidiform mole associated and imprinted.

    Journal: International Journal of Molecular Medicine

    Article Title: Identification of the key genes and long non-coding RNAs in ankylosing spondylitis using RNA sequencing

    doi: 10.3892/ijmm.2018.4038

    Figure Lengend Snippet: Validation of the expression of selected DEmRNAs and DElncRNAs between patients with AS and the normal controls in the GSE25101 dataset. The x-axis presents the AS and normal control groups and the y-axis presents the expression levels. (A) The selected DEmRNAs and (B) The selected DElncRNAs. AS, ankylosing spondylitis; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA; CARD11, caspase recruitment domain-containing protein 11; DNMT1, DNA methyltransferase 1; PDCD1, programmed cell death 1; PLCG1, phospholipase Cγ1; MANSC1, MANSC domain containing 1; ATP2A2, adenosine triphosphatase sarcoplasmic/endoplasmic reticulum Ca 2+ transporting 2; MZF1, myeloid zinc finger 1; CECR7, cat eye syndrome chromosome region candidate 7; HYMAI, hydatidiform mole associated and imprinted.

    Article Snippet: Validation of the expression of DEmRNAs and DElncRNAs using the GSE73754 and GSE25101 datasets The mRNA expression profile of 52 patients with AS and 20 normal controls (Canadian cohort) in the GSE73754 dataset (GPL10558 Illumina HumanHT-12 V4.0 expression beadchip), which was downloaded from the Gene Expression Omnibus (GEO; www.ncbi.nlm.nih.gov/gds ).

    Techniques: Expressing

    Top 15 most significantly enriched KEGG pathways of DEmRNAs co-expressed with DElncRNAs. The x-axis presents the number of DEmRNAs enriched in KEGG pathways and the y-axis presents the KEGG pathways. KEGG, Kyoto Encyclopedia of Genes and Genomes; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA; FDR, false discovery rate.

    Journal: International Journal of Molecular Medicine

    Article Title: Identification of the key genes and long non-coding RNAs in ankylosing spondylitis using RNA sequencing

    doi: 10.3892/ijmm.2018.4038

    Figure Lengend Snippet: Top 15 most significantly enriched KEGG pathways of DEmRNAs co-expressed with DElncRNAs. The x-axis presents the number of DEmRNAs enriched in KEGG pathways and the y-axis presents the KEGG pathways. KEGG, Kyoto Encyclopedia of Genes and Genomes; DEmRNA, differentially expressed mRNA; DElncRNA, differentially expressed long non-coding RNA; FDR, false discovery rate.

    Article Snippet: Validation of the expression of DEmRNAs and DElncRNAs using the GSE73754 and GSE25101 datasets The mRNA expression profile of 52 patients with AS and 20 normal controls (Canadian cohort) in the GSE73754 dataset (GPL10558 Illumina HumanHT-12 V4.0 expression beadchip), which was downloaded from the Gene Expression Omnibus (GEO; www.ncbi.nlm.nih.gov/gds ).

    Techniques:

    Validation of HIV-1 regulated host cellular factors at the RNA and protein level. ( A ) Independent validations of randomly selected differentially regulated mRNAs from transcriptome analyses. qRT-PCR was performed to validate the expression of selected mRNAs using specific primer and probe pairs. Fold increase/decrease was calculated based on normalization to RPLPO. Average fold change for each mRNA represents fold change obtained from independent donors (N = 5 per group). ( B ) Inflammatory factors released by HIV-1 infected PBMC compared to uninfected control cells. Expression of CCL2, CCL8, CXCL5, IL6 and IL8 was monitored by ELISA in supernatants obtained from PBMCs infected with CXCR4-coreceptor utilizing virus (NL43), CCR5-coreceptor utilizing virus (YU2) or mock infected PBMCs (n = 7). * = p

    Journal: BMC Infectious Diseases

    Article Title: MicroRNA regulation and its effects on cellular transcriptome in Human Immunodeficiency Virus-1 (HIV-1) infected individuals with distinct viral load and CD4 cell counts

    doi: 10.1186/1471-2334-13-250

    Figure Lengend Snippet: Validation of HIV-1 regulated host cellular factors at the RNA and protein level. ( A ) Independent validations of randomly selected differentially regulated mRNAs from transcriptome analyses. qRT-PCR was performed to validate the expression of selected mRNAs using specific primer and probe pairs. Fold increase/decrease was calculated based on normalization to RPLPO. Average fold change for each mRNA represents fold change obtained from independent donors (N = 5 per group). ( B ) Inflammatory factors released by HIV-1 infected PBMC compared to uninfected control cells. Expression of CCL2, CCL8, CXCL5, IL6 and IL8 was monitored by ELISA in supernatants obtained from PBMCs infected with CXCR4-coreceptor utilizing virus (NL43), CCR5-coreceptor utilizing virus (YU2) or mock infected PBMCs (n = 7). * = p

    Article Snippet: mRNA profiling and data analysis For whole genome transcriptome analysis, we used Illumina HT-12 V4 array bead chips (Illumina, Inc., San Diego, CA, USA) for mRNA profiling of the different groups (Control, LVL and HVL) in the study.

    Techniques: Quantitative RT-PCR, Expressing, Infection, Enzyme-linked Immunosorbent Assay