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NanoString Technologies Inc mrna molecules
hetIL-15 enhances the production of XCL1 by tumor resident CD8 + T and NK cells. (A) Heatmap, represented as Z-score centered and rescaled, of the genes included in the GO:0002548 monocyte migration and chemotaxis pathway from control (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. Significantly upregulated genes in hetIL-15-treated mice in comparison to control mice are depicted. (B) Evaluation of the chemokine XCL1 in MC38 tumors. The panel shows the tumor <t>mRNA</t> counts for Xcl1 as determined by the <t>Nanostring</t> technologies in both PBS treated (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. (C) MC38 tumor lysates from either PBS-treated (n=11) or hetIL-15-treated (n=10) mice were assessed for XCL1 concentration by ELISA. (D) Flow cytometric analysis of XCL1 production by NK and CD8 + T cells. Histogram overlays show the expression of XCL1 by intratumoral NK and CD8 + T cells from a representative hetIL-15 (blue) and PBS (black) treated mouse. Solid gray histogram shows non-staining control. The XCL1 geometric mean fluorescent intensity (MFI) in the tumor-infiltrating NK and CD8 + T cells from each therapeutic group (n=6) are shown in the right panel. Bars represent mean±SEM. P values are from Mann-Whitney U test. hetIL-15, heterodimeric interleukin-15; NK, natural killer; SEM, Standard error of the mean.
Mrna Molecules, supplied by NanoString Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 2 article reviews
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1) Product Images from "Heterodimeric IL-15 delays tumor growth and promotes intratumoral CTL and dendritic cell accumulation by a cytokine network involving XCL1, IFN-γ, CXCL9 and CXCL10"

Article Title: Heterodimeric IL-15 delays tumor growth and promotes intratumoral CTL and dendritic cell accumulation by a cytokine network involving XCL1, IFN-γ, CXCL9 and CXCL10

Journal: Journal for Immunotherapy of Cancer

doi: 10.1136/jitc-2020-000599

hetIL-15 enhances the production of XCL1 by tumor resident CD8 + T and NK cells. (A) Heatmap, represented as Z-score centered and rescaled, of the genes included in the GO:0002548 monocyte migration and chemotaxis pathway from control (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. Significantly upregulated genes in hetIL-15-treated mice in comparison to control mice are depicted. (B) Evaluation of the chemokine XCL1 in MC38 tumors. The panel shows the tumor mRNA counts for Xcl1 as determined by the Nanostring technologies in both PBS treated (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. (C) MC38 tumor lysates from either PBS-treated (n=11) or hetIL-15-treated (n=10) mice were assessed for XCL1 concentration by ELISA. (D) Flow cytometric analysis of XCL1 production by NK and CD8 + T cells. Histogram overlays show the expression of XCL1 by intratumoral NK and CD8 + T cells from a representative hetIL-15 (blue) and PBS (black) treated mouse. Solid gray histogram shows non-staining control. The XCL1 geometric mean fluorescent intensity (MFI) in the tumor-infiltrating NK and CD8 + T cells from each therapeutic group (n=6) are shown in the right panel. Bars represent mean±SEM. P values are from Mann-Whitney U test. hetIL-15, heterodimeric interleukin-15; NK, natural killer; SEM, Standard error of the mean.
Figure Legend Snippet: hetIL-15 enhances the production of XCL1 by tumor resident CD8 + T and NK cells. (A) Heatmap, represented as Z-score centered and rescaled, of the genes included in the GO:0002548 monocyte migration and chemotaxis pathway from control (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. Significantly upregulated genes in hetIL-15-treated mice in comparison to control mice are depicted. (B) Evaluation of the chemokine XCL1 in MC38 tumors. The panel shows the tumor mRNA counts for Xcl1 as determined by the Nanostring technologies in both PBS treated (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. (C) MC38 tumor lysates from either PBS-treated (n=11) or hetIL-15-treated (n=10) mice were assessed for XCL1 concentration by ELISA. (D) Flow cytometric analysis of XCL1 production by NK and CD8 + T cells. Histogram overlays show the expression of XCL1 by intratumoral NK and CD8 + T cells from a representative hetIL-15 (blue) and PBS (black) treated mouse. Solid gray histogram shows non-staining control. The XCL1 geometric mean fluorescent intensity (MFI) in the tumor-infiltrating NK and CD8 + T cells from each therapeutic group (n=6) are shown in the right panel. Bars represent mean±SEM. P values are from Mann-Whitney U test. hetIL-15, heterodimeric interleukin-15; NK, natural killer; SEM, Standard error of the mean.

Techniques Used: Migration, Chemotaxis Assay, Mouse Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Staining, MANN-WHITNEY

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    NanoString Technologies Inc mrna molecules
    hetIL-15 enhances the production of XCL1 by tumor resident CD8 + T and NK cells. (A) Heatmap, represented as Z-score centered and rescaled, of the genes included in the GO:0002548 monocyte migration and chemotaxis pathway from control (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. Significantly upregulated genes in hetIL-15-treated mice in comparison to control mice are depicted. (B) Evaluation of the chemokine XCL1 in MC38 tumors. The panel shows the tumor <t>mRNA</t> counts for Xcl1 as determined by the <t>Nanostring</t> technologies in both PBS treated (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. (C) MC38 tumor lysates from either PBS-treated (n=11) or hetIL-15-treated (n=10) mice were assessed for XCL1 concentration by ELISA. (D) Flow cytometric analysis of XCL1 production by NK and CD8 + T cells. Histogram overlays show the expression of XCL1 by intratumoral NK and CD8 + T cells from a representative hetIL-15 (blue) and PBS (black) treated mouse. Solid gray histogram shows non-staining control. The XCL1 geometric mean fluorescent intensity (MFI) in the tumor-infiltrating NK and CD8 + T cells from each therapeutic group (n=6) are shown in the right panel. Bars represent mean±SEM. P values are from Mann-Whitney U test. hetIL-15, heterodimeric interleukin-15; NK, natural killer; SEM, Standard error of the mean.
    Mrna Molecules, supplied by NanoString Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrna molecules/product/NanoString Technologies Inc
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mrna molecules - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    hetIL-15 enhances the production of XCL1 by tumor resident CD8 + T and NK cells. (A) Heatmap, represented as Z-score centered and rescaled, of the genes included in the GO:0002548 monocyte migration and chemotaxis pathway from control (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. Significantly upregulated genes in hetIL-15-treated mice in comparison to control mice are depicted. (B) Evaluation of the chemokine XCL1 in MC38 tumors. The panel shows the tumor mRNA counts for Xcl1 as determined by the Nanostring technologies in both PBS treated (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. (C) MC38 tumor lysates from either PBS-treated (n=11) or hetIL-15-treated (n=10) mice were assessed for XCL1 concentration by ELISA. (D) Flow cytometric analysis of XCL1 production by NK and CD8 + T cells. Histogram overlays show the expression of XCL1 by intratumoral NK and CD8 + T cells from a representative hetIL-15 (blue) and PBS (black) treated mouse. Solid gray histogram shows non-staining control. The XCL1 geometric mean fluorescent intensity (MFI) in the tumor-infiltrating NK and CD8 + T cells from each therapeutic group (n=6) are shown in the right panel. Bars represent mean±SEM. P values are from Mann-Whitney U test. hetIL-15, heterodimeric interleukin-15; NK, natural killer; SEM, Standard error of the mean.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Heterodimeric IL-15 delays tumor growth and promotes intratumoral CTL and dendritic cell accumulation by a cytokine network involving XCL1, IFN-γ, CXCL9 and CXCL10

    doi: 10.1136/jitc-2020-000599

    Figure Lengend Snippet: hetIL-15 enhances the production of XCL1 by tumor resident CD8 + T and NK cells. (A) Heatmap, represented as Z-score centered and rescaled, of the genes included in the GO:0002548 monocyte migration and chemotaxis pathway from control (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. Significantly upregulated genes in hetIL-15-treated mice in comparison to control mice are depicted. (B) Evaluation of the chemokine XCL1 in MC38 tumors. The panel shows the tumor mRNA counts for Xcl1 as determined by the Nanostring technologies in both PBS treated (n=5) and hetIL-15-treated (n=6) MC38-bearing mice. (C) MC38 tumor lysates from either PBS-treated (n=11) or hetIL-15-treated (n=10) mice were assessed for XCL1 concentration by ELISA. (D) Flow cytometric analysis of XCL1 production by NK and CD8 + T cells. Histogram overlays show the expression of XCL1 by intratumoral NK and CD8 + T cells from a representative hetIL-15 (blue) and PBS (black) treated mouse. Solid gray histogram shows non-staining control. The XCL1 geometric mean fluorescent intensity (MFI) in the tumor-infiltrating NK and CD8 + T cells from each therapeutic group (n=6) are shown in the right panel. Bars represent mean±SEM. P values are from Mann-Whitney U test. hetIL-15, heterodimeric interleukin-15; NK, natural killer; SEM, Standard error of the mean.

    Article Snippet: The mRNA molecules were counted with the NanoString nCounter at the Laboratory of Molecular Technology (Advanced Technology Program, Frederick National Laboratory).

    Techniques: Migration, Chemotaxis Assay, Mouse Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Staining, MANN-WHITNEY