mrna magnetic isolation module  (New England Biolabs)


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    Name:
    NEBNext Poly A mRNA Magnetic Isolation Module
    Description:
    NEBNext Poly A mRNA Magnetic Isolation Module 96 rxns
    Catalog Number:
    e7490l
    Price:
    242
    Size:
    96 rxns
    Category:
    mRNA Purification Kits
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    Structured Review

    New England Biolabs mrna magnetic isolation module
    NEBNext Poly A mRNA Magnetic Isolation Module
    NEBNext Poly A mRNA Magnetic Isolation Module 96 rxns
    https://www.bioz.com/result/mrna magnetic isolation module/product/New England Biolabs
    Average 90 stars, based on 403 article reviews
    Price from $9.99 to $1999.99
    mrna magnetic isolation module - by Bioz Stars, 2020-01
    90/100 stars

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    Amplification:

    Article Title: Spatially resolved, highly multiplexed RNA profiling in single cells
    Article Snippet: .. Total RNA was extracted from IMR90 cells cultured as above using the Zymo Quick RNA MiniPrep kit (R1054) according to the manufacturer’s instructions. polyA RNA was then selected (NEB; E7490), and a sequencing library was constructed using the NEBNext Ultra RNA library preparation kit (NEB; E7530), amplified with custom oligonucleotides, and 150-bp reads were obtained from on a MiSeq. ..

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530). .. After Not I digestion, the library DNA was purified for PCR amplification (16 cycles) (more details in “Step-by-step Holo-Seq protocols” (Additional file )).

    Synthesized:

    Article Title: Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp. tritici during Conidiation
    Article Snippet: Libraries Construction and Sequencing Before mRNA sequencing, poly (A) mRNA was isolated from total RNA using the NEB-E7490 Poly (A) mRNA Magnetic Isolation Kit (NEB, United States), and then fragmented by the Fragmentation Kit (Ambion, Austin, TX, United States). cDNA libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s recommendations. .. Briefly, the first-strand and the second of cDNA were synthesized using M-MuLV reverse transcriptase and DNA polymerase I (Invitrogen), respectively.

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: .. Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen). .. Quantitative reverse transcription-PCR (qRT-PCR) was performed using an ABI Prism 7900HT machine (Applied Biosystems, Foster City, CA).

    Construct:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490). .. Illumina-compatible RNA-libraries were constructed by using the NEBNext Ultra RNALibrary Prep Kit for Illumina (E7530) as well as the NEBNext Multiplex Oligos for Illumina (E7335) following the distributors instruction manuals.

    Article Title: Spatially resolved, highly multiplexed RNA profiling in single cells
    Article Snippet: .. Total RNA was extracted from IMR90 cells cultured as above using the Zymo Quick RNA MiniPrep kit (R1054) according to the manufacturer’s instructions. polyA RNA was then selected (NEB; E7490), and a sequencing library was constructed using the NEBNext Ultra RNA library preparation kit (NEB; E7530), amplified with custom oligonucleotides, and 150-bp reads were obtained from on a MiSeq. ..

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: Three libraries labeled A, M and B were constructed from RNA extracted from the apical, middle and basal stem segments, respectively. .. The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490, E7530, E7420, and E7300) for the mRNA library, the directional total RNA library, and the small RNA library, respectively. .. Sequencing library construction of mRNAs from a single cell A single cell was lysed at 95 °C for 5 min in 0.1% BSA/PBS with 100 ng of the carrier RNA mixture.

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530). .. After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion.

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The libraries were constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530), before PCR enrichment, the DNA was digested by Not I at 37 °C for 2 h or Cas9 nuclease with sgRNA mix at 37 °C for 2 h. .. In vitro digestion of the DNA template of carrier RNA using CRISPR/Cas9 The sgRNA sites on the DNA template were selected using the Optimized CRISPR Design website.

    Article Title: Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp. tritici during Conidiation
    Article Snippet: .. Libraries Construction and Sequencing Before mRNA sequencing, poly (A) mRNA was isolated from total RNA using the NEB-E7490 Poly (A) mRNA Magnetic Isolation Kit (NEB, United States), and then fragmented by the Fragmentation Kit (Ambion, Austin, TX, United States). cDNA libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s recommendations. .. Briefly, the first-strand and the second of cDNA were synthesized using M-MuLV reverse transcriptase and DNA polymerase I (Invitrogen), respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490). .. Multiplexed RNA libraries were mixed in equilibrium according to their Qubit (Life Technologies) measurements, fragment distribution profiles on the Advanced Anayltical Fragment Analyzer and quantification of library 5′-and 3′- ends by Real Time PCR.

    Multiplex Assay:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490). .. Illumina-compatible RNA-libraries were constructed by using the NEBNext Ultra RNALibrary Prep Kit for Illumina (E7530) as well as the NEBNext Multiplex Oligos for Illumina (E7335) following the distributors instruction manuals.

    Expressing:

    Article Title: A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation
    Article Snippet: From 2μg of total RNA from each biological replicate (4 MRTF-A−/− and 4 MRTF-A+/+ , age matched), Library preparation was done using a NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760) and mRNA enrichment was done by NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490), according to manufacturer's protocols. .. Fastq files are deposited at NCBI's Gene Expression Omnibus (GEO) database with BioProject ID PRJNA535475 and BioSample accession number SAMN11506732 .

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen). .. Results were normalized to hypoxanthine phosphoribosyltransferase expression and wild-type, untreated control animals.

    Flow Cytometry:

    Article Title: A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation
    Article Snippet: mRNA Sequencing The sequencing was performed at the Biomedicum Functional Genomics Unit (FuGU) of the University of Helsinki with the Illumina NextSeq500 using a NextSeq Mid Output 150 cycle flow cell. .. From 2μg of total RNA from each biological replicate (4 MRTF-A−/− and 4 MRTF-A+/+ , age matched), Library preparation was done using a NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760) and mRNA enrichment was done by NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490), according to manufacturer's protocols.

    Ligation:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530). .. After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion.

    Article Title: Phenotypic and molecular analysis of the effect of 20-hydroxyecdysone on the human filarial parasite Brugia malayi
    Article Snippet: The NEBNext poly(A) mRNA Magnetic Isolation Module (New England Biolabs Inc., Ipswich, MA, USA; # E7490) was used to isolate intact poly(A)+ RNA from each previously isolated total RNA preparations. .. End repair and dA tailing of cDNA library was performed, immediately followed by adaptor ligation.

    Biomarker Assay:

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: Construction of the six libraries and RNA-Seq analysis were performed by Biomarker Biotechnology Corporation (Beijing, China). .. The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Cell Culture:

    Article Title: Spatially resolved, highly multiplexed RNA profiling in single cells
    Article Snippet: .. Total RNA was extracted from IMR90 cells cultured as above using the Zymo Quick RNA MiniPrep kit (R1054) according to the manufacturer’s instructions. polyA RNA was then selected (NEB; E7490), and a sequencing library was constructed using the NEBNext Ultra RNA library preparation kit (NEB; E7530), amplified with custom oligonucleotides, and 150-bp reads were obtained from on a MiSeq. ..

    Polymerase Chain Reaction:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530). .. After Not I digestion, the library DNA was purified for PCR amplification (16 cycles) (more details in “Step-by-step Holo-Seq protocols” (Additional file )).

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The libraries were constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530), before PCR enrichment, the DNA was digested by Not I at 37 °C for 2 h or Cas9 nuclease with sgRNA mix at 37 °C for 2 h. .. In vitro digestion of the DNA template of carrier RNA using CRISPR/Cas9 The sgRNA sites on the DNA template were selected using the Optimized CRISPR Design website.

    Article Title: Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp. tritici during Conidiation
    Article Snippet: Libraries Construction and Sequencing Before mRNA sequencing, poly (A) mRNA was isolated from total RNA using the NEB-E7490 Poly (A) mRNA Magnetic Isolation Kit (NEB, United States), and then fragmented by the Fragmentation Kit (Ambion, Austin, TX, United States). cDNA libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s recommendations. .. Next, these fragments were enriched by PCR amplifications and library quality was assessed by the Agilent Bioanalyzer 2100 System.

    Article Title: Lnc-mg is a long non-coding RNA that promotes myogenesis
    Article Snippet: Then polyA+ RNA fraction and polyA- RNA fraction were isolated by using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, NEB E7490S/L). .. In addition, the amount of lnc-mg was examined in PCR assay with polyA+ RNA fraction and polyA-RNA fraction, respectively.

    Immunofluorescence:

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: Paragraph title: Quantitative RT-PCR, immunoblotting, immunofluorescence, and immunohistochemistry. ... Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen).

    RNA Sequencing Assay:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: Paragraph title: High-throughput sequencing of RNA (RNA-Seq) ... Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490).

    Article Title: Spatially resolved, highly multiplexed RNA profiling in single cells
    Article Snippet: Paragraph title: Bulk RNA sequencing ... Total RNA was extracted from IMR90 cells cultured as above using the Zymo Quick RNA MiniPrep kit (R1054) according to the manufacturer’s instructions. polyA RNA was then selected (NEB; E7490), and a sequencing library was constructed using the NEBNext Ultra RNA library preparation kit (NEB; E7530), amplified with custom oligonucleotides, and 150-bp reads were obtained from on a MiSeq.

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: Paragraph title: RNA extraction, library construction and RNA-Seq ... The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Article Title: Coordinate regulation of alternative pre-mRNA splicing events by the human RNA chaperone proteins hnRNPA1 and DDX5
    Article Snippet: Paragraph title: RNA-seq library preparation and sequencing ... After RNA isolation using RNeasy minikit (Qiagen, 74104) followed by 30 min of DNase treatment (Ambion, AM2238) at 37°C, poly(A)+ RNA transcript was isolated [NEBNext poly(A) mRNA magnetic isolation module; New England Biolabs, E7490] from 1 µg of total RNA for RNA library preparation and sequencing using NEBNext Ultra directional RNA library preparation kit for Illumina (New England Biolabs, E7420S) according to the manufacturer's instruction.

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: Paragraph title: Not I and Cas9 digestion of bulk RNA-Seq libraries ... The libraries were constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530), before PCR enrichment, the DNA was digested by Not I at 37 °C for 2 h or Cas9 nuclease with sgRNA mix at 37 °C for 2 h.

    Isolation:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: .. Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490). .. Illumina-compatible RNA-libraries were constructed by using the NEBNext Ultra RNALibrary Prep Kit for Illumina (E7530) as well as the NEBNext Multiplex Oligos for Illumina (E7335) following the distributors instruction manuals.

    Article Title: A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation
    Article Snippet: .. From 2μg of total RNA from each biological replicate (4 MRTF-A−/− and 4 MRTF-A+/+ , age matched), Library preparation was done using a NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760) and mRNA enrichment was done by NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490), according to manufacturer's protocols. ..

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: RNA extraction, library construction and RNA-Seq Total RNA from each sample was isolated using CTAB plus the OMEGA Plant RNA isolation kit as described previously [ ]. .. The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Article Title: Coordinate regulation of alternative pre-mRNA splicing events by the human RNA chaperone proteins hnRNPA1 and DDX5
    Article Snippet: .. After RNA isolation using RNeasy minikit (Qiagen, 74104) followed by 30 min of DNase treatment (Ambion, AM2238) at 37°C, poly(A)+ RNA transcript was isolated [NEBNext poly(A) mRNA magnetic isolation module; New England Biolabs, E7490] from 1 µg of total RNA for RNA library preparation and sequencing using NEBNext Ultra directional RNA library preparation kit for Illumina (New England Biolabs, E7420S) according to the manufacturer's instruction. .. The samples were sequenced on an Illumina HiSeq 2500 with 100-bp paired-end reads at the Vincent J. Coates Genomics Sequencing Laboratory at the University of California at Berkeley.

    Article Title: Phenotypic and molecular analysis of the effect of 20-hydroxyecdysone on the human filarial parasite Brugia malayi
    Article Snippet: .. The NEBNext poly(A) mRNA Magnetic Isolation Module (New England Biolabs Inc., Ipswich, MA, USA; # E7490) was used to isolate intact poly(A)+ RNA from each previously isolated total RNA preparations. .. The eluted RNA was used for first and second strand cDNA synthesis using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA; # E7530).

    Article Title: Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp. tritici during Conidiation
    Article Snippet: .. Libraries Construction and Sequencing Before mRNA sequencing, poly (A) mRNA was isolated from total RNA using the NEB-E7490 Poly (A) mRNA Magnetic Isolation Kit (NEB, United States), and then fragmented by the Fragmentation Kit (Ambion, Austin, TX, United States). cDNA libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s recommendations. .. Briefly, the first-strand and the second of cDNA were synthesized using M-MuLV reverse transcriptase and DNA polymerase I (Invitrogen), respectively.

    Article Title: Lnc-mg is a long non-coding RNA that promotes myogenesis
    Article Snippet: .. Then polyA+ RNA fraction and polyA- RNA fraction were isolated by using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, NEB E7490S/L). .. In addition, the amount of lnc-mg was examined in PCR assay with polyA+ RNA fraction and polyA-RNA fraction, respectively.

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: .. Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen). .. Quantitative reverse transcription-PCR (qRT-PCR) was performed using an ABI Prism 7900HT machine (Applied Biosystems, Foster City, CA).

    Immunohistochemistry:

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: Paragraph title: Quantitative RT-PCR, immunoblotting, immunofluorescence, and immunohistochemistry. ... Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen).

    Labeling:

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: Three libraries labeled A, M and B were constructed from RNA extracted from the apical, middle and basal stem segments, respectively. .. The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Purification:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530). .. After Not I digestion, the library DNA was purified for PCR amplification (16 cycles) (more details in “Step-by-step Holo-Seq protocols” (Additional file )).

    Article Title: Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp. tritici during Conidiation
    Article Snippet: Libraries Construction and Sequencing Before mRNA sequencing, poly (A) mRNA was isolated from total RNA using the NEB-E7490 Poly (A) mRNA Magnetic Isolation Kit (NEB, United States), and then fragmented by the Fragmentation Kit (Ambion, Austin, TX, United States). cDNA libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s recommendations. .. After NEBNext adaptors were ligated to the adenylated 3′ ends of cDNA, fragments of approximately 500 bp in length were purified and selected with the AMPure XP system (Beckman Coulter, Beverly, MA, United States).

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: .. Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen). .. Quantitative reverse transcription-PCR (qRT-PCR) was performed using an ABI Prism 7900HT machine (Applied Biosystems, Foster City, CA).

    Sequencing:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: Paragraph title: High-throughput sequencing of RNA (RNA-Seq) ... Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490).

    Article Title: A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation
    Article Snippet: Paragraph title: mRNA Sequencing ... From 2μg of total RNA from each biological replicate (4 MRTF-A−/− and 4 MRTF-A+/+ , age matched), Library preparation was done using a NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760) and mRNA enrichment was done by NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490), according to manufacturer's protocols.

    Article Title: Spatially resolved, highly multiplexed RNA profiling in single cells
    Article Snippet: .. Total RNA was extracted from IMR90 cells cultured as above using the Zymo Quick RNA MiniPrep kit (R1054) according to the manufacturer’s instructions. polyA RNA was then selected (NEB; E7490), and a sequencing library was constructed using the NEBNext Ultra RNA library preparation kit (NEB; E7530), amplified with custom oligonucleotides, and 150-bp reads were obtained from on a MiSeq. ..

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490, E7530, E7420, and E7300) for the mRNA library, the directional total RNA library, and the small RNA library, respectively. .. Sequencing library construction of mRNAs from a single cell A single cell was lysed at 95 °C for 5 min in 0.1% BSA/PBS with 100 ng of the carrier RNA mixture.

    Article Title: Coordinate regulation of alternative pre-mRNA splicing events by the human RNA chaperone proteins hnRNPA1 and DDX5
    Article Snippet: .. After RNA isolation using RNeasy minikit (Qiagen, 74104) followed by 30 min of DNase treatment (Ambion, AM2238) at 37°C, poly(A)+ RNA transcript was isolated [NEBNext poly(A) mRNA magnetic isolation module; New England Biolabs, E7490] from 1 µg of total RNA for RNA library preparation and sequencing using NEBNext Ultra directional RNA library preparation kit for Illumina (New England Biolabs, E7420S) according to the manufacturer's instruction. .. The samples were sequenced on an Illumina HiSeq 2500 with 100-bp paired-end reads at the Vincent J. Coates Genomics Sequencing Laboratory at the University of California at Berkeley.

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The sequencing library was constructed following the manufacturer’s protocol using NEBNext kits (E7490 & E7530). .. After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion.

    Article Title: Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp. tritici during Conidiation
    Article Snippet: .. Libraries Construction and Sequencing Before mRNA sequencing, poly (A) mRNA was isolated from total RNA using the NEB-E7490 Poly (A) mRNA Magnetic Isolation Kit (NEB, United States), and then fragmented by the Fragmentation Kit (Ambion, Austin, TX, United States). cDNA libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s recommendations. .. Briefly, the first-strand and the second of cDNA were synthesized using M-MuLV reverse transcriptase and DNA polymerase I (Invitrogen), respectively.

    Quantitative RT-PCR:

    Article Title: Chromatin Profiling Reveals Regulatory Network Shifts and a Protective Role for Hepatocyte Nuclear Factor 4α during Colitis
    Article Snippet: Paragraph title: Quantitative RT-PCR, immunoblotting, immunofluorescence, and immunohistochemistry. ... Five micrograms of total RNA from control and DSS-inflamed colon was used to purify poly(A) mRNA with the NEBNext poly(A) mRNA magnetic isolation module (E7490S/L; New England Biolabs). cDNA was synthesized from purified Poly(A) mRNA by using oligo(dT) primers (SuperScript III first-strand synthesis kit; Invitrogen).

    cDNA Library Assay:

    Article Title: Phenotypic and molecular analysis of the effect of 20-hydroxyecdysone on the human filarial parasite Brugia malayi
    Article Snippet: The NEBNext poly(A) mRNA Magnetic Isolation Module (New England Biolabs Inc., Ipswich, MA, USA; # E7490) was used to isolate intact poly(A)+ RNA from each previously isolated total RNA preparations. .. End repair and dA tailing of cDNA library was performed, immediately followed by adaptor ligation.

    RNA Extraction:

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: Paragraph title: RNA extraction, library construction and RNA-Seq ... The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Functional Assay:

    Article Title: A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation
    Article Snippet: mRNA Sequencing The sequencing was performed at the Biomedicum Functional Genomics Unit (FuGU) of the University of Helsinki with the Illumina NextSeq500 using a NextSeq Mid Output 150 cycle flow cell. .. From 2μg of total RNA from each biological replicate (4 MRTF-A−/− and 4 MRTF-A+/+ , age matched), Library preparation was done using a NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760) and mRNA enrichment was done by NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490), according to manufacturer's protocols.

    Agarose Gel Electrophoresis:

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: The RNA samples were checked for integrity on a 1.2% agarose gel and quantified using a Nanodrop 1000 spectrophotometer. .. The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    Article Title: Lnc-mg is a long non-coding RNA that promotes myogenesis
    Article Snippet: Next, the RNA quality was assessed by formaldehyde agarose gel electrophoresis, quantified spectrophotometrically and Agilent 2200 Bioanalyzer (Agilent). .. Then polyA+ RNA fraction and polyA- RNA fraction were isolated by using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, NEB E7490S/L).

    Spectrophotometry:

    Article Title: Transcriptomic Analysis of Multipurpose Timber Yielding Tree Neolamarckia cadamba during Xylogenesis Using RNA-Seq
    Article Snippet: The RNA samples were checked for integrity on a 1.2% agarose gel and quantified using a Nanodrop 1000 spectrophotometer. .. The mRNA enrichment and library construction were carried out according to protocol of NEB kit (E7490, E6110, E7500).

    High Throughput Screening Assay:

    Article Title: Queuine links translational control in eukaryotes to a micronutrient from bacteria
    Article Snippet: Paragraph title: High-throughput sequencing of RNA (RNA-Seq) ... Inc; Ankeny Iowa, USA), the mRNA was isolated from high quality total RNA extracts by using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490).

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    New England Biolabs mrna magnetic isolation module
    Cleavage of 3′-UTR regions results in autonomous uncapped <t>RNA</t> fragments. a RNA-seq read coverage ( y -axis) for Ssr1 , Bcl2 , and Rab2a in mouse T cells 12 , B cells 14 , and brain tissue 15 , demonstrating a gap in read coverage (marked by arrows) at 3′-UTRs, as well as uneven levels of RNA upstream and downstream of the gap site. b Three sets of primers were used per gene for qRT-PCR amplification: upstream (blue rectangle), downstream (red), and across the RNA-seq coverage gap (green). Bar plots (bottom) visualize the relative percentage of cytoplasmic (black) and nuclear (gray) expression of each amplicon out of its total (cytoplasm+nuclear) expression. c A model for post-transcriptional processing of <t>mRNA:</t> mRNA is cleaved at an APA site, resulting in a capped “body” and an uncapped 3′-UTR “tail,” sensitive to terminator 5′-phosphate-dependent exonuclease (TEX) treatment. d qRT-PCR analysis shows cytoplasmic degradation of the “tail” unit (in red) compared to the “body” unit (in blue) following TEX treatment. * p
    Mrna Magnetic Isolation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cleavage of 3′-UTR regions results in autonomous uncapped RNA fragments. a RNA-seq read coverage ( y -axis) for Ssr1 , Bcl2 , and Rab2a in mouse T cells 12 , B cells 14 , and brain tissue 15 , demonstrating a gap in read coverage (marked by arrows) at 3′-UTRs, as well as uneven levels of RNA upstream and downstream of the gap site. b Three sets of primers were used per gene for qRT-PCR amplification: upstream (blue rectangle), downstream (red), and across the RNA-seq coverage gap (green). Bar plots (bottom) visualize the relative percentage of cytoplasmic (black) and nuclear (gray) expression of each amplicon out of its total (cytoplasm+nuclear) expression. c A model for post-transcriptional processing of mRNA: mRNA is cleaved at an APA site, resulting in a capped “body” and an uncapped 3′-UTR “tail,” sensitive to terminator 5′-phosphate-dependent exonuclease (TEX) treatment. d qRT-PCR analysis shows cytoplasmic degradation of the “tail” unit (in red) compared to the “body” unit (in blue) following TEX treatment. * p

    Journal: Nature Communications

    Article Title: Post-transcriptional 3´-UTR cleavage of mRNA transcripts generates thousands of stable uncapped autonomous RNA fragments

    doi: 10.1038/s41467-017-02099-7

    Figure Lengend Snippet: Cleavage of 3′-UTR regions results in autonomous uncapped RNA fragments. a RNA-seq read coverage ( y -axis) for Ssr1 , Bcl2 , and Rab2a in mouse T cells 12 , B cells 14 , and brain tissue 15 , demonstrating a gap in read coverage (marked by arrows) at 3′-UTRs, as well as uneven levels of RNA upstream and downstream of the gap site. b Three sets of primers were used per gene for qRT-PCR amplification: upstream (blue rectangle), downstream (red), and across the RNA-seq coverage gap (green). Bar plots (bottom) visualize the relative percentage of cytoplasmic (black) and nuclear (gray) expression of each amplicon out of its total (cytoplasm+nuclear) expression. c A model for post-transcriptional processing of mRNA: mRNA is cleaved at an APA site, resulting in a capped “body” and an uncapped 3′-UTR “tail,” sensitive to terminator 5′-phosphate-dependent exonuclease (TEX) treatment. d qRT-PCR analysis shows cytoplasmic degradation of the “tail” unit (in red) compared to the “body” unit (in blue) following TEX treatment. * p

    Article Snippet: 3′-RNA capping and pulldown RNA was poly(A) selected using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB-E7490S).

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Amplification, Expressing