peroxidase polymer anti mouse  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories peroxidase polymer anti mouse
    Peroxidase Polymer Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase polymer anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase polymer anti mouse - by Bioz Stars, 2024-05
    93/100 stars

    Images

    peroxidase polymer anti mouse  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories peroxidase polymer anti mouse
    Peroxidase Polymer Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase polymer anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase polymer anti mouse - by Bioz Stars, 2024-05
    93/100 stars

    Images

    secondary hrp conjugated polymers  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories secondary hrp conjugated polymers
    Secondary Hrp Conjugated Polymers, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary hrp conjugated polymers/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary hrp conjugated polymers - by Bioz Stars, 2024-05
    93/100 stars

    Images

    anti mouse immpress  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories anti mouse immpress
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Anti Mouse Immpress, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse immpress/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse immpress - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "BNIP3 Regulates AT101 [(-)-Gossypol] Induced Death in Malignant Peripheral Nerve Sheath Tumor Cells"

    Article Title: BNIP3 Regulates AT101 [(-)-Gossypol] Induced Death in Malignant Peripheral Nerve Sheath Tumor Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0096733

    AT101 treated MPNST cells show a concentration-dependent increase in BNIP3 protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Figure Legend Snippet: AT101 treated MPNST cells show a concentration-dependent increase in BNIP3 protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.

    Techniques Used: Concentration Assay, Western Blot, Immunocytochemistry, Expressing, Transfection

    T265-2c cells treated with AT101 (10 µM for 24 hours) demonstrate increased levels of HIF-1α protein (A), but AT101 treated MPNST cells do not show a significant change in HIF-1α mRNA levels (B) relative to untreated cells. AT101 causes an increase in HIF-1α protein levels in the nuclear fraction of T265-2c cells (C). T265-2c cells transfected with HIF-1α siRNA show decreased levels of HIF-1α protein (D) and BNIP3 protein expression (E) upon treatment with AT101, relative to cells transfected with non-target siRNA. # = Not significant.
    Figure Legend Snippet: T265-2c cells treated with AT101 (10 µM for 24 hours) demonstrate increased levels of HIF-1α protein (A), but AT101 treated MPNST cells do not show a significant change in HIF-1α mRNA levels (B) relative to untreated cells. AT101 causes an increase in HIF-1α protein levels in the nuclear fraction of T265-2c cells (C). T265-2c cells transfected with HIF-1α siRNA show decreased levels of HIF-1α protein (D) and BNIP3 protein expression (E) upon treatment with AT101, relative to cells transfected with non-target siRNA. # = Not significant.

    Techniques Used: Transfection, Expressing

    AT101 (15 µM for 24 hours) treatment increases transferrin receptor 1 (TfR1) protein levels and decreases ferritin protein levels in MPNST cells (A), which are reversed by addition of ferric citrate to the media. Supplementation of the culture media with ferric citrate (100 µM) also abrogates AT101-induced HIF-1α, BNIP3 and LC3II protein expression (B) and cytotoxicity in T265-2c and 90-8 cells (C, D). MPNST cells are not rescued from ABT737 (15 µM) induced cytotoxicity by supplementation of culture media with ferric citrate (100 µM) (E, F). DFO (50 µM for 24 hours) was used as a positive control for iron chelation. * p -value <0.05. # = Not significant.
    Figure Legend Snippet: AT101 (15 µM for 24 hours) treatment increases transferrin receptor 1 (TfR1) protein levels and decreases ferritin protein levels in MPNST cells (A), which are reversed by addition of ferric citrate to the media. Supplementation of the culture media with ferric citrate (100 µM) also abrogates AT101-induced HIF-1α, BNIP3 and LC3II protein expression (B) and cytotoxicity in T265-2c and 90-8 cells (C, D). MPNST cells are not rescued from ABT737 (15 µM) induced cytotoxicity by supplementation of culture media with ferric citrate (100 µM) (E, F). DFO (50 µM for 24 hours) was used as a positive control for iron chelation. * p -value <0.05. # = Not significant.

    Techniques Used: Expressing, Positive Control

    AT101 and DFO (0.1–10 µM) can directly bind iron and reduce the absorbance due to free iron, whereas ABT737, at similar concentrations, does not bind iron and thus does not alter the absorbance * p -value <0.05. (A). Schematic representation of the proposed mechanism of action of AT101 in MPNSTs (B). In normoxia, Prolyl-hydroxylases (PHDs) play a critical role in HIF-1α degradation. AT101 chelates intracellular iron, which is required for the proper function of PHDs. Loss of function of the PHDs leads to an accumulation of HIF-1α, which translocates to the nucleus and transcriptionally upregulates BNIP3 expression leading to cell death.
    Figure Legend Snippet: AT101 and DFO (0.1–10 µM) can directly bind iron and reduce the absorbance due to free iron, whereas ABT737, at similar concentrations, does not bind iron and thus does not alter the absorbance * p -value <0.05. (A). Schematic representation of the proposed mechanism of action of AT101 in MPNSTs (B). In normoxia, Prolyl-hydroxylases (PHDs) play a critical role in HIF-1α degradation. AT101 chelates intracellular iron, which is required for the proper function of PHDs. Loss of function of the PHDs leads to an accumulation of HIF-1α, which translocates to the nucleus and transcriptionally upregulates BNIP3 expression leading to cell death.

    Techniques Used: Expressing

    peroxidase conjugated anti mouse  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories peroxidase conjugated anti mouse
    Peroxidase Conjugated Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated anti mouse - by Bioz Stars, 2024-05
    93/100 stars

    Images

    peroxidase conjugated secondary antibodies against mouse  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories peroxidase conjugated secondary antibodies against mouse
    Peroxidase Conjugated Secondary Antibodies Against Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibodies against mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibodies against mouse - by Bioz Stars, 2024-05
    93/100 stars

    Images

    immpress anti mouse  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories immpress anti mouse
    Immpress Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpress anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpress anti mouse - by Bioz Stars, 2024-05
    93/100 stars

    Images

    peroxidase immpress reagent kit anti mouse immunoglobulins  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories peroxidase immpress reagent kit anti mouse immunoglobulins
    Peroxidase Immpress Reagent Kit Anti Mouse Immunoglobulins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase immpress reagent kit anti mouse immunoglobulins/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase immpress reagent kit anti mouse immunoglobulins - by Bioz Stars, 2024-05
    93/100 stars

    Images

    peroxidase  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories peroxidase
    Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase - by Bioz Stars, 2024-05
    93/100 stars

    Images

    immpress polymer detection reagent  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories immpress polymer detection reagent
    Immpress Polymer Detection Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpress polymer detection reagent/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpress polymer detection reagent - by Bioz Stars, 2024-05
    93/100 stars

    Images

    immpress mouse  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Vector Laboratories immpress mouse
    Immpress Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpress mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpress mouse - by Bioz Stars, 2024-05
    93/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Vector Laboratories peroxidase polymer anti mouse
    Peroxidase Polymer Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase polymer anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase polymer anti mouse - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories secondary hrp conjugated polymers
    Secondary Hrp Conjugated Polymers, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary hrp conjugated polymers/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary hrp conjugated polymers - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories anti mouse immpress
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Anti Mouse Immpress, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse immpress/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse immpress - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories peroxidase conjugated anti mouse
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Peroxidase Conjugated Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated anti mouse - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories peroxidase conjugated secondary antibodies against mouse
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Peroxidase Conjugated Secondary Antibodies Against Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibodies against mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibodies against mouse - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories immpress anti mouse
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Immpress Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpress anti mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpress anti mouse - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories peroxidase immpress reagent kit anti mouse immunoglobulins
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Peroxidase Immpress Reagent Kit Anti Mouse Immunoglobulins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase immpress reagent kit anti mouse immunoglobulins/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase immpress reagent kit anti mouse immunoglobulins - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories peroxidase
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories immpress polymer detection reagent
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Immpress Polymer Detection Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpress polymer detection reagent/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpress polymer detection reagent - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Vector Laboratories immpress mouse
    AT101 treated MPNST cells show a concentration-dependent increase <t>in</t> <t>BNIP3</t> protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.
    Immpress Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpress mouse/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpress mouse - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    AT101 treated MPNST cells show a concentration-dependent increase in BNIP3 protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.

    Journal: PLoS ONE

    Article Title: BNIP3 Regulates AT101 [(-)-Gossypol] Induced Death in Malignant Peripheral Nerve Sheath Tumor Cells

    doi: 10.1371/journal.pone.0096733

    Figure Lengend Snippet: AT101 treated MPNST cells show a concentration-dependent increase in BNIP3 protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p -value <0.05.

    Article Snippet: HRP–conjugated anti-rabbit Super Picture (Invitrogen; used for LC3), and anti-mouse ImmPRESS (Vector Laboratories; used for BNIP3) were both used at 1∶100 dilutions.

    Techniques: Concentration Assay, Western Blot, Immunocytochemistry, Expressing, Transfection

    T265-2c cells treated with AT101 (10 µM for 24 hours) demonstrate increased levels of HIF-1α protein (A), but AT101 treated MPNST cells do not show a significant change in HIF-1α mRNA levels (B) relative to untreated cells. AT101 causes an increase in HIF-1α protein levels in the nuclear fraction of T265-2c cells (C). T265-2c cells transfected with HIF-1α siRNA show decreased levels of HIF-1α protein (D) and BNIP3 protein expression (E) upon treatment with AT101, relative to cells transfected with non-target siRNA. # = Not significant.

    Journal: PLoS ONE

    Article Title: BNIP3 Regulates AT101 [(-)-Gossypol] Induced Death in Malignant Peripheral Nerve Sheath Tumor Cells

    doi: 10.1371/journal.pone.0096733

    Figure Lengend Snippet: T265-2c cells treated with AT101 (10 µM for 24 hours) demonstrate increased levels of HIF-1α protein (A), but AT101 treated MPNST cells do not show a significant change in HIF-1α mRNA levels (B) relative to untreated cells. AT101 causes an increase in HIF-1α protein levels in the nuclear fraction of T265-2c cells (C). T265-2c cells transfected with HIF-1α siRNA show decreased levels of HIF-1α protein (D) and BNIP3 protein expression (E) upon treatment with AT101, relative to cells transfected with non-target siRNA. # = Not significant.

    Article Snippet: HRP–conjugated anti-rabbit Super Picture (Invitrogen; used for LC3), and anti-mouse ImmPRESS (Vector Laboratories; used for BNIP3) were both used at 1∶100 dilutions.

    Techniques: Transfection, Expressing

    AT101 (15 µM for 24 hours) treatment increases transferrin receptor 1 (TfR1) protein levels and decreases ferritin protein levels in MPNST cells (A), which are reversed by addition of ferric citrate to the media. Supplementation of the culture media with ferric citrate (100 µM) also abrogates AT101-induced HIF-1α, BNIP3 and LC3II protein expression (B) and cytotoxicity in T265-2c and 90-8 cells (C, D). MPNST cells are not rescued from ABT737 (15 µM) induced cytotoxicity by supplementation of culture media with ferric citrate (100 µM) (E, F). DFO (50 µM for 24 hours) was used as a positive control for iron chelation. * p -value <0.05. # = Not significant.

    Journal: PLoS ONE

    Article Title: BNIP3 Regulates AT101 [(-)-Gossypol] Induced Death in Malignant Peripheral Nerve Sheath Tumor Cells

    doi: 10.1371/journal.pone.0096733

    Figure Lengend Snippet: AT101 (15 µM for 24 hours) treatment increases transferrin receptor 1 (TfR1) protein levels and decreases ferritin protein levels in MPNST cells (A), which are reversed by addition of ferric citrate to the media. Supplementation of the culture media with ferric citrate (100 µM) also abrogates AT101-induced HIF-1α, BNIP3 and LC3II protein expression (B) and cytotoxicity in T265-2c and 90-8 cells (C, D). MPNST cells are not rescued from ABT737 (15 µM) induced cytotoxicity by supplementation of culture media with ferric citrate (100 µM) (E, F). DFO (50 µM for 24 hours) was used as a positive control for iron chelation. * p -value <0.05. # = Not significant.

    Article Snippet: HRP–conjugated anti-rabbit Super Picture (Invitrogen; used for LC3), and anti-mouse ImmPRESS (Vector Laboratories; used for BNIP3) were both used at 1∶100 dilutions.

    Techniques: Expressing, Positive Control

    AT101 and DFO (0.1–10 µM) can directly bind iron and reduce the absorbance due to free iron, whereas ABT737, at similar concentrations, does not bind iron and thus does not alter the absorbance * p -value <0.05. (A). Schematic representation of the proposed mechanism of action of AT101 in MPNSTs (B). In normoxia, Prolyl-hydroxylases (PHDs) play a critical role in HIF-1α degradation. AT101 chelates intracellular iron, which is required for the proper function of PHDs. Loss of function of the PHDs leads to an accumulation of HIF-1α, which translocates to the nucleus and transcriptionally upregulates BNIP3 expression leading to cell death.

    Journal: PLoS ONE

    Article Title: BNIP3 Regulates AT101 [(-)-Gossypol] Induced Death in Malignant Peripheral Nerve Sheath Tumor Cells

    doi: 10.1371/journal.pone.0096733

    Figure Lengend Snippet: AT101 and DFO (0.1–10 µM) can directly bind iron and reduce the absorbance due to free iron, whereas ABT737, at similar concentrations, does not bind iron and thus does not alter the absorbance * p -value <0.05. (A). Schematic representation of the proposed mechanism of action of AT101 in MPNSTs (B). In normoxia, Prolyl-hydroxylases (PHDs) play a critical role in HIF-1α degradation. AT101 chelates intracellular iron, which is required for the proper function of PHDs. Loss of function of the PHDs leads to an accumulation of HIF-1α, which translocates to the nucleus and transcriptionally upregulates BNIP3 expression leading to cell death.

    Article Snippet: HRP–conjugated anti-rabbit Super Picture (Invitrogen; used for LC3), and anti-mouse ImmPRESS (Vector Laboratories; used for BNIP3) were both used at 1∶100 dilutions.

    Techniques: Expressing