mp65 cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC mp65 cells
    Mp65 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 cells - by Bioz Stars, 2023-01
    94/100 stars

    Images

    mp65  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mp65
    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, <t>MP65)</t> treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).
    Mp65, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 - by Bioz Stars, 2023-01
    86/100 stars

    Images

    1) Product Images from "Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth"

    Article Title: Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth

    Journal: Cancers

    doi: 10.3390/cancers14246247

    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, MP65) treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).
    Figure Legend Snippet: NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, MP65) treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).

    Techniques Used: Migration, Expressing, Immunohistochemical staining, Staining, Western Blot, Colony Assay, Microscopy

    Shows percent cells gated in subG1 phase with /without treatment with NT157 . The percent of cells in cell cycle phases was obtained by counting propidium iodide-stained cells after 48 h of NT157 treatment. At the higher NT157 concentration we see an accumulation of subG1 cells compared to the untreated controls.
    Figure Legend Snippet: Shows percent cells gated in subG1 phase with /without treatment with NT157 . The percent of cells in cell cycle phases was obtained by counting propidium iodide-stained cells after 48 h of NT157 treatment. At the higher NT157 concentration we see an accumulation of subG1 cells compared to the untreated controls.

    Techniques Used: Concentration Assay

    mp65 cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC mp65 cells
    Mp65 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 cells - by Bioz Stars, 2023-01
    94/100 stars

    Images

    mp65  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mp65
    ( A ) TCGA dataset analysis shows expression of IRS-1 mRNA in UM tumors (indicated by an arrow) in comparison to 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody to detect IRS-1 expression. ( C ) Western blot analysis of cell extracts from UM cell lines (OMM1, Mel285,92.1, MEL20-06-039) treated with NT157 (1 μM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, <t>MP65</t> and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 μM) or without NT157 treatment shows NT157 dose-dependent decrease in cell survival. The bar graphs represent percentage of cell survival and are a mean ± SD of three independent experiments. ( E ) Representative images of crystal violet staining shows fewer number of colonies formed by UM cell lines (MEL202, 92.1, MM28 MP38, MP41, MP46 and MP65) with NT157 treatment (1 and 2.5 μM) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039 and MP65) shows a dose-dependent increase of number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 μM) vs. without treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 μM) increase in cleaved PARP and decrease in caspase3 levels detected by respective antibodies in UM cell lines (Mel202, Mel270, 92.1 and Mel20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MP65 and MM28) shows reduction in migration in cells treated with NT157 (2.5 μM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40X magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/ field were plotted as bar graphs.
    Mp65, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 - by Bioz Stars, 2023-01
    86/100 stars

    Images

    1) Product Images from "Targeting IRS-1/2 in uveal melanoma inhibits in vitro cell growth, survival and migration, and in vivo tumor growth"

    Article Title: Targeting IRS-1/2 in uveal melanoma inhibits in vitro cell growth, survival and migration, and in vivo tumor growth

    Journal: bioRxiv

    doi: 10.1101/2022.10.26.513928

    ( A ) TCGA dataset analysis shows expression of IRS-1 mRNA in UM tumors (indicated by an arrow) in comparison to 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody to detect IRS-1 expression. ( C ) Western blot analysis of cell extracts from UM cell lines (OMM1, Mel285,92.1, MEL20-06-039) treated with NT157 (1 μM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65 and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 μM) or without NT157 treatment shows NT157 dose-dependent decrease in cell survival. The bar graphs represent percentage of cell survival and are a mean ± SD of three independent experiments. ( E ) Representative images of crystal violet staining shows fewer number of colonies formed by UM cell lines (MEL202, 92.1, MM28 MP38, MP41, MP46 and MP65) with NT157 treatment (1 and 2.5 μM) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039 and MP65) shows a dose-dependent increase of number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 μM) vs. without treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 μM) increase in cleaved PARP and decrease in caspase3 levels detected by respective antibodies in UM cell lines (Mel202, Mel270, 92.1 and Mel20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MP65 and MM28) shows reduction in migration in cells treated with NT157 (2.5 μM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40X magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/ field were plotted as bar graphs.
    Figure Legend Snippet: ( A ) TCGA dataset analysis shows expression of IRS-1 mRNA in UM tumors (indicated by an arrow) in comparison to 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody to detect IRS-1 expression. ( C ) Western blot analysis of cell extracts from UM cell lines (OMM1, Mel285,92.1, MEL20-06-039) treated with NT157 (1 μM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65 and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 μM) or without NT157 treatment shows NT157 dose-dependent decrease in cell survival. The bar graphs represent percentage of cell survival and are a mean ± SD of three independent experiments. ( E ) Representative images of crystal violet staining shows fewer number of colonies formed by UM cell lines (MEL202, 92.1, MM28 MP38, MP41, MP46 and MP65) with NT157 treatment (1 and 2.5 μM) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039 and MP65) shows a dose-dependent increase of number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 μM) vs. without treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 μM) increase in cleaved PARP and decrease in caspase3 levels detected by respective antibodies in UM cell lines (Mel202, Mel270, 92.1 and Mel20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MP65 and MM28) shows reduction in migration in cells treated with NT157 (2.5 μM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40X magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/ field were plotted as bar graphs.

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Western Blot, Migration, Microscopy

    mp65  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mp65
    Mp65, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 - by Bioz Stars, 2023-01
    86/100 stars

    Images

    sporotrichum atcc 3299  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC sporotrichum atcc 3299
    Sporotrichum Atcc 3299, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sporotrichum atcc 3299/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sporotrichum atcc 3299 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    shewanella japonica kmm 3299 t  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC shewanella japonica kmm 3299 t
    Shewanella Japonica Kmm 3299 T, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shewanella japonica kmm 3299 t/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shewanella japonica kmm 3299 t - by Bioz Stars, 2023-01
    94/100 stars

    Images

    mp65  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mp65
    LOC100132707 expression was increased in metastatic melanoma. ( A ) Heat map from Cancer RNA-Seq Nexus Database showed the different expression lncRNAs among the primary melanoma and metastatic melanoma. ( B ) TCGA database demonstrated that LOC100132707 expression was increased in metastatic melanoma. ( C ) Survivorship curve was used to analyze the effects of different expression levels of LOC100132707 on the overall survival of patients with melanoma. ( D ) RT-PCR assay was carried out to determine the mRNA expressions of LOC100132707 in UM cell lines. (*P<0.05, compared with the <t>MP65</t> group).
    Mp65, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 - by Bioz Stars, 2023-01
    86/100 stars

    Images

    1) Product Images from "Knockdown of Long Non-Coding RNA LOC100132707 Inhibits the Migration of Uveal Melanoma Cells via Silencing JAK2"

    Article Title: Knockdown of Long Non-Coding RNA LOC100132707 Inhibits the Migration of Uveal Melanoma Cells via Silencing JAK2

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S266596

    LOC100132707 expression was increased in metastatic melanoma. ( A ) Heat map from Cancer RNA-Seq Nexus Database showed the different expression lncRNAs among the primary melanoma and metastatic melanoma. ( B ) TCGA database demonstrated that LOC100132707 expression was increased in metastatic melanoma. ( C ) Survivorship curve was used to analyze the effects of different expression levels of LOC100132707 on the overall survival of patients with melanoma. ( D ) RT-PCR assay was carried out to determine the mRNA expressions of LOC100132707 in UM cell lines. (*P<0.05, compared with the MP65 group).
    Figure Legend Snippet: LOC100132707 expression was increased in metastatic melanoma. ( A ) Heat map from Cancer RNA-Seq Nexus Database showed the different expression lncRNAs among the primary melanoma and metastatic melanoma. ( B ) TCGA database demonstrated that LOC100132707 expression was increased in metastatic melanoma. ( C ) Survivorship curve was used to analyze the effects of different expression levels of LOC100132707 on the overall survival of patients with melanoma. ( D ) RT-PCR assay was carried out to determine the mRNA expressions of LOC100132707 in UM cell lines. (*P<0.05, compared with the MP65 group).

    Techniques Used: Expressing, RNA Sequencing Assay, Reverse Transcription Polymerase Chain Reaction

    Assessment of the expressions of LOC100132707-related genes in UM cell lines. ( A – F ) RT-PCR assay was used to test the mRNA levels of XRN1, PARP14, JAK2, DDX60, BUB1 and SAMD9L in MM28, MP38, MP46 and MP65 cell lines. (*P<0.05, compared with the MP65 group).
    Figure Legend Snippet: Assessment of the expressions of LOC100132707-related genes in UM cell lines. ( A – F ) RT-PCR assay was used to test the mRNA levels of XRN1, PARP14, JAK2, DDX60, BUB1 and SAMD9L in MM28, MP38, MP46 and MP65 cell lines. (*P<0.05, compared with the MP65 group).

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    LOC100132707 overexpression enhanced the migration and invasion of UM cells. ( A ) RT-PCR was used to detect the expression of LOC100132707 in sh-LOC100132707-transfected MM28 cells. ( B and C ) Transwell chambers were used to detect the effects of LOC100132707 downregulation on MM28 cell invasion and migration. ( D ) The expression of LOC100132707 was tested by RT-PCR in OE- LOC100132707-transfected or OE-NC-transfected MP65 cells. ( E and F ) Transwell chambers were used to detect the effects of LOC100132707 upregulation on MP65 cell invasion and migration. (n=3, *P<0.05, compared with the sh-NC/OE-NC group).
    Figure Legend Snippet: LOC100132707 overexpression enhanced the migration and invasion of UM cells. ( A ) RT-PCR was used to detect the expression of LOC100132707 in sh-LOC100132707-transfected MM28 cells. ( B and C ) Transwell chambers were used to detect the effects of LOC100132707 downregulation on MM28 cell invasion and migration. ( D ) The expression of LOC100132707 was tested by RT-PCR in OE- LOC100132707-transfected or OE-NC-transfected MP65 cells. ( E and F ) Transwell chambers were used to detect the effects of LOC100132707 upregulation on MP65 cell invasion and migration. (n=3, *P<0.05, compared with the sh-NC/OE-NC group).

    Techniques Used: Over Expression, Migration, Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection

    LOC100132707 overexpression enhanced the migration and invasion of UM cells via upregulating JAK2. ( A ) Western blotting assay was used to detect the protein expression of JAK2 in OE-JAK2/OE-NC-transfected MM28 cells (*P<0.05, compared with the OE-NC group). ( B – D ) Transwell chambers were used to detect the effects of LOC100132707/JAK2 axis on MM28 cell invasion and migration (*P<0.05, compared with sh-NC + OE-NC group; # P<0.05, compared with sh-LOC100132707 + OE-NC group). ( E ) Western blotting assay was used to detect the protein expression of JAK2 in sh-JAK2/sh-NC-transfected MP65 cells (*P<0.05, compared with the sh-NC group). ( F – H ) Transwell chambers were used to detect the effects of LOC100132707/JAK2 axis on MP65 cell migration and invasion (n=3, *P<0.05, compared with the OE-NC + sh-NC group; # P<0.05, compared with the OE-LOC100132707 + sh-NC group).
    Figure Legend Snippet: LOC100132707 overexpression enhanced the migration and invasion of UM cells via upregulating JAK2. ( A ) Western blotting assay was used to detect the protein expression of JAK2 in OE-JAK2/OE-NC-transfected MM28 cells (*P<0.05, compared with the OE-NC group). ( B – D ) Transwell chambers were used to detect the effects of LOC100132707/JAK2 axis on MM28 cell invasion and migration (*P<0.05, compared with sh-NC + OE-NC group; # P<0.05, compared with sh-LOC100132707 + OE-NC group). ( E ) Western blotting assay was used to detect the protein expression of JAK2 in sh-JAK2/sh-NC-transfected MP65 cells (*P<0.05, compared with the sh-NC group). ( F – H ) Transwell chambers were used to detect the effects of LOC100132707/JAK2 axis on MP65 cell migration and invasion (n=3, *P<0.05, compared with the OE-NC + sh-NC group; # P<0.05, compared with the OE-LOC100132707 + sh-NC group).

    Techniques Used: Over Expression, Migration, Western Blot, Expressing, Transfection

    mp65  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC mp65
    Mp65, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    mp65 cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC mp65 cells
    Mp65 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 cells - by Bioz Stars, 2023-01
    94/100 stars

    Images

    km873060 shewanella japonica strain kmm 3299  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC km873060 shewanella japonica strain kmm 3299
    Km873060 Shewanella Japonica Strain Kmm 3299, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/km873060 shewanella japonica strain kmm 3299/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    km873060 shewanella japonica strain kmm 3299 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    ATCC mp65 cells
    Mp65 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 cells - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    mp65  (ATCC)
    86
    ATCC mp65
    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, <t>MP65)</t> treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).
    Mp65, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mp65/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mp65 - by Bioz Stars, 2023-01
    86/100 stars
      Buy from Supplier

    94
    ATCC sporotrichum atcc 3299
    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, <t>MP65)</t> treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).
    Sporotrichum Atcc 3299, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sporotrichum atcc 3299/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sporotrichum atcc 3299 - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    ATCC shewanella japonica kmm 3299 t
    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, <t>MP65)</t> treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).
    Shewanella Japonica Kmm 3299 T, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shewanella japonica kmm 3299 t/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shewanella japonica kmm 3299 t - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    ATCC km873060 shewanella japonica strain kmm 3299
    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, <t>MP65)</t> treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).
    Km873060 Shewanella Japonica Strain Kmm 3299, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/km873060 shewanella japonica strain kmm 3299/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    km873060 shewanella japonica strain kmm 3299 - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, MP65) treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).

    Journal: Cancers

    Article Title: Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth

    doi: 10.3390/cancers14246247

    Figure Lengend Snippet: NT157 treatment reduces IRS-1 levels leading to a reduction of cell viability, migration, and induction of apoptosis in UM cell lines. ( A ) TCGA database analysis shows high expression of IRS-1 mRNA in UM tumors (indicated by an arrow) among 33 cancer (red dots) and corresponding normal (green dots) tissues. ( B ) Immunohistochemical staining of matched primary (eye) and metastatic (liver) UM tumor tissues using IRS-1 antibody detects IRS-1 expression (purple) in both eye and liver (both 2× and 20× magnifications are shown). ( C ) Western blot analysis of cell extracts from UM cell lines (92.1, OMM2.5, OMM1, MEL20-06-039, MP65) treated with NT157 (1 µM) and probed with anti-IRS-1 antibody shows IRS-1 protein levels decrease in UM cell lines with NT157 treatment for 48 h. ( D ) Quantification of percent cell survival using MTT-based assays in UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with (0.05, 0.1, 0.25, 0.5, 1.0, 2.5 µM) or without NT157 treatment (72 h) shows NT157 dose-dependent decrease in cell survival. The bar graphs represent a percentage of cell survival and are a mean ± SD of three independent experiments. p-values were calculated by comparing untreated controls with NT157 treatment doses ( * p < 0.05; ** p < 0.01; *** p < 0.001). ( E ) Colony formation assay: Representative images of crystal violet staining shows fewer colonies formed by UM cell lines (MP46, MM28, MEL202, 92.1, MP65, and MP41) with NT157 treatment (1 and 2.5 µM for seven days) compared to untreated controls. ( F ) Representative FACS histograms of DNA content detected by propidium iodide staining to detect cell cycle status in UM cell lines (MP46, MM28 and MEL20-06-039, and MP65) show a dose-dependent increase in the number of cells in G0/G1 phase with NT157 treatment (1 and 2.5 µM for 72 h) vs. no treatment. ( G ) Western blot analysis of total cell extracts shows NT157 dose-dependent (1 and 2.5 µM for 72 h) increase in cleaved PARP and decrease in caspase 3 levels detected by respective antibodies in UM cell lines (MEL202, MEL270, 92.1, and MEL20-06-039) compared to untreated controls. ( H ) Quantification of cell migration in UM cell lines (OMM1, 92.1, MEL202, MP65, and MM28) shows a reduction in migration of cells treated with NT157 (2.5 µM) compared to untreated controls. An average of five fields of cells/filter were counted under a microscope with 40× magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p -values were calculated by comparing untreated vs. NT157 treated cells ( * p < 0.05; ** p < 0.01; *** p < 0.001).

    Article Snippet: UM cell lines obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) were: MM28 (#CRL-3295), MP38 (#CRL-3296), MP41 (#CRL-3297), MP46 (#CRL-3298), MP65 (#CRL-3299).

    Techniques: Migration, Expressing, Immunohistochemical staining, Staining, Western Blot, Colony Assay, Microscopy

    Shows percent cells gated in subG1 phase with /without treatment with NT157 . The percent of cells in cell cycle phases was obtained by counting propidium iodide-stained cells after 48 h of NT157 treatment. At the higher NT157 concentration we see an accumulation of subG1 cells compared to the untreated controls.

    Journal: Cancers

    Article Title: Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth

    doi: 10.3390/cancers14246247

    Figure Lengend Snippet: Shows percent cells gated in subG1 phase with /without treatment with NT157 . The percent of cells in cell cycle phases was obtained by counting propidium iodide-stained cells after 48 h of NT157 treatment. At the higher NT157 concentration we see an accumulation of subG1 cells compared to the untreated controls.

    Article Snippet: UM cell lines obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) were: MM28 (#CRL-3295), MP38 (#CRL-3296), MP41 (#CRL-3297), MP46 (#CRL-3298), MP65 (#CRL-3299).

    Techniques: Concentration Assay