Journal: ACS chemical biology
Article Title: Sugar-Binding Proteins from Fish: Selection of High Affnity "Lambodies" That Recognize Biomedically Relevant Glycans
doi: 10.1021/cb300399s
Figure Lengend Snippet: Lambody staining of human normal and cancer tissues. (A) Normal lung (tumor adjacent), (B) lung large cell carcinoma, (C, D) lung squamous cell carcinoma, (E) normal colon (tumor adjacent), (F) colon adenocarcinoma, (G) normal prostate, and (H) prostate adenocarcinoma. Images were scanned at 20x magnification. Tissue microarray slides were incubated with 5 μ g/mL of VLRB.aGPA.23-mFc, and the immune complexes were detected with anti-mouse IgG-HRP and DAB substrate (brown) and then counterstained with hematoxylin (blue).
Article Snippet: After washing in PBST, the lambodyantigen complexes were detected with ImmPRESS anti-mouse IgG-HRP and ImmPACT DAB peroxidase substrate and counter-stained with hematoxylin (Vector Laboratories).
Techniques: Staining, Microarray, Incubation