mouse tnfsf11 rankl picokine elisa kit  (Boster Bio)


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    Boster Bio mouse tnfsf11 rankl picokine elisa kit
    Mouse Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse tnfsf11 rankl picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mouse tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-10
    94/100 stars

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  • 94
    Boster Bio tnfsf11 rankl picokine elisa kit
    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and <t>RANKL.</t> After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of <t>RANKL</t> and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p
    Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfsf11 rankl picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    94
    Boster Bio elisa kit
    Influence of <t>Syndecan-1</t> deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by <t>ELISA.</t> Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p
    Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Journal: MedComm

    Article Title: Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling

    doi: 10.1002/mco2.131

    Figure Lengend Snippet: Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Article Snippet: ATP production was quantified using a CellTiter‐Glo Luminescent Cell Viability Assay Kit (Promega, USA)., Serum levels of OPG and RANKL were measured by mouse OPG ELISA Kit (Boster Biological Technology) and TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) according to the manufacturer's instructions.

    Techniques: Mouse Assay, Staining, Isolation, Cell Culture, Incubation, Micro-CT

    Dopaminergic neurons suppress osteoclastogenesis. ( A ) The percentage of osteoclast precursor cells, characterized by the expression of the cell surface markers c-kit and c-fms, together with the absence or dull expression of CD11b, in bone marrow of control and MPTP-injected mice. Representative data (left) and quantification (right, control, n = 8; MPTP, n = 8) are shown. ( B ) Osteoclast differentiation of BMMs obtained from control and MPTP-injected mice. Left: representative images obtained from more than three independent experiments. Right: quantification of TRAP + multinuclear osteoclasts (MNC) (control, n = 6; MPTP, n = 6). ( C ) mRNA expression of NFATc1 of cells obtained from control and MPTP-injected mice 2 days after RANKL stimulation (control, n = 8; MPTP, n = 8). ( D ) Osteoclast differentiation in presence of 2% serum obtained from control and MPTP-injected mice and the effect of anti-prolactin neutralizing antibody in the presence of MPTP serum (left). Left: representative images obtained more than three independent experiments. Right: quantification of TRAP + MNC (control, n = 6; MPTP, n = 6; MPTP+anti-prolactin Ab, n = 6). ( E ) mRNA expression of NFATc1 of cells 2 days after RANKL stimulation in the presence of mouse serum (control, n = 8; MPTP, n = 8). ( F ) Ratio of RANKL/OPG in serum from control and MPTP-injected mice (control, n = 8; MPTP, n = 8). Statistical analyses were performed using Student’s t-test ( A – C , E , F ) or ANOVA with Dunnett’s multiple-comparison test ( D ). * P

    Journal: Scientific Reports

    Article Title: Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson’s disease model mice

    doi: 10.1038/s41598-019-50336-4

    Figure Lengend Snippet: Dopaminergic neurons suppress osteoclastogenesis. ( A ) The percentage of osteoclast precursor cells, characterized by the expression of the cell surface markers c-kit and c-fms, together with the absence or dull expression of CD11b, in bone marrow of control and MPTP-injected mice. Representative data (left) and quantification (right, control, n = 8; MPTP, n = 8) are shown. ( B ) Osteoclast differentiation of BMMs obtained from control and MPTP-injected mice. Left: representative images obtained from more than three independent experiments. Right: quantification of TRAP + multinuclear osteoclasts (MNC) (control, n = 6; MPTP, n = 6). ( C ) mRNA expression of NFATc1 of cells obtained from control and MPTP-injected mice 2 days after RANKL stimulation (control, n = 8; MPTP, n = 8). ( D ) Osteoclast differentiation in presence of 2% serum obtained from control and MPTP-injected mice and the effect of anti-prolactin neutralizing antibody in the presence of MPTP serum (left). Left: representative images obtained more than three independent experiments. Right: quantification of TRAP + MNC (control, n = 6; MPTP, n = 6; MPTP+anti-prolactin Ab, n = 6). ( E ) mRNA expression of NFATc1 of cells 2 days after RANKL stimulation in the presence of mouse serum (control, n = 8; MPTP, n = 8). ( F ) Ratio of RANKL/OPG in serum from control and MPTP-injected mice (control, n = 8; MPTP, n = 8). Statistical analyses were performed using Student’s t-test ( A – C , E , F ) or ANOVA with Dunnett’s multiple-comparison test ( D ). * P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The concentrations of RANKL, OPG and Hcy in obtained mouse serum were determined using a Mouse TNFSF11/RANKL EZ-Set™ ELISA Kit, Mouse OPG (TNFRSF11B) PicoKine™ ELISA Kit (Boster Biological Technology) and Hcy ELISA Kit (Cosmo Bio), respectively, according to the individual manufacturer’s instructions.

    Techniques: Expressing, Injection, Mouse Assay

    Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p

    Journal: bioRxiv

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1101/852590

    Figure Lengend Snippet: Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Concentration Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM+ medium (day 7). A : mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. B, C : The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann-Whitney-U test, *p

    Journal: bioRxiv

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1101/852590

    Figure Lengend Snippet: Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM+ medium (day 7). A : mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. B, C : The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann-Whitney-U test, *p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM + medium (day 7). ( A ) mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. ( B,C ) The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann–Whitney- U test, * p

    Journal: Scientific Reports

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1038/s41598-020-77510-3

    Figure Lengend Snippet: Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM + medium (day 7). ( A ) mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. ( B,C ) The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann–Whitney- U test, * p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. ( A–C ) Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B,C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal–Wallis test with Dunn´s post hoc test, n = 9–13 per group. * p

    Journal: Scientific Reports

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1038/s41598-020-77510-3

    Figure Lengend Snippet: Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. ( A–C ) Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B,C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal–Wallis test with Dunn´s post hoc test, n = 9–13 per group. * p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Concentration Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay