Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mice with Intracranial Gliomas

doi: 10.12659/MSM.903098

Figure Lengend Snippet: Plasma levels of IFN-γ and TGF-β in the mice belonging to the various groups. ( A ) Plasma IFN-γ levels in mice receiving combined treatment were markedly increased. ( B ) Plasma TGF-β levels were decreased in the combined treatment group. * P <0.05; ** P <0.01, compared with control mice.

Article Snippet: The venous blood was collected from the eye socket vein of the mice, and centrifuged for 8 min at 800× g . The concentrations of interferon-γ (IFN-γ, pg/mL) and transforming growth factor-β (TGF-β, pg/mL) were then measured using an ELISA kit (catalog#EK0373; Boster, WuHan, HuBei, China) and TGF-β kit (catalog#EK0515; Boster), according to the manufacturers’ instructions.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Activation of the STAT5 Signaling Pathway by Yiqi Jiedu Formula Induces Regulatory T Cell-Mediated Alleviation of Corneal Immunopathological Damage in Mice With Recurrent Herpes Simplex Keratitis

doi: 10.3389/fphar.2021.790787

Figure Lengend Snippet: Effects of YQJD treatment on the ocular surface score and cytokine gene expression. (A) Representative images of stained mice ocular surfaces 3 days after UV irradiation. Sodium fluorescein staining was carried out as follows: the operator grasped the mouse’s ears and neck skin with the thumb and index finger of the left hand. The ring finger and little finger of the left hand were used to clamp the skin on the back and the tail, while the right hand was kept steady and with minimal shaking. The cornea was stained with 10 g/L sodium fluorescein and examined under a slit lamp microscope. (B) Ocular surface scores of mice 3 days after UV irradiation. (C–F) Expression of (C) Il-17 , (D) Tgf-β , (E) Cd11c , and (F) Il-4 in the mouse cornea. Data are expressed as the mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the Re group; # p < 0.05 vs. the Naive group; n = 6 for ocular surface scoring; n = 3 for RT-qPCR experiments.

Article Snippet: Specific kits against IL-10 (Boster Bio, EK0417) and TGF-β (Boster Bio, EK0515) were used to detect cytokine levels before and after HSK recurrence in serum and spleen cells of mice.

Techniques: Expressing, Staining, Irradiation, Microscopy, Quantitative RT-PCR

Journal: Frontiers in Pharmacology

Article Title: Activation of the STAT5 Signaling Pathway by Yiqi Jiedu Formula Induces Regulatory T Cell-Mediated Alleviation of Corneal Immunopathological Damage in Mice With Recurrent Herpes Simplex Keratitis

doi: 10.3389/fphar.2021.790787

Figure Lengend Snippet: Effects of YQJD treatment on the immune response in peripheral blood and spleen of recurrent HSK mice. (A) Levels of CD4 + CD25 + Foxp3 + Tregs in peripheral blood. (B) Levels of CD4 + CD25 + Foxp3 + Tregs in the spleen. (C) Expression of IL-10 in peripheral blood. (D) Expression of IL-10 in spleen cells. (E) Expression of TGF-β in peripheral blood. (F) Expression of TGF-β in spleen cells. The data are expressed as the mean ± SD of five independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the Re group; #p < 0.05 and ##p < 0.01 vs . the Naive group.

Article Snippet: Specific kits against IL-10 (Boster Bio, EK0417) and TGF-β (Boster Bio, EK0515) were used to detect cytokine levels before and after HSK recurrence in serum and spleen cells of mice.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Macrophage Foam Cell-Targeting Immunization Attenuates Atherosclerosis

doi: 10.3389/fimmu.2018.03127

Figure Lengend Snippet: FCs vaccination modulates cytokines in initial atherosclerosis. The levels of IFN-γ (A) , IL-6 (B) , MCP-1 (C) , and TGF-β1 (D) in immune ApoE −/− mouse plasma were determined at 1:2 dilution by ELISA according to the manufacturer's protocol. n = 8 in each group. Aortic roots were sectioned and stained with MCP-1. Representative photographs of MCP-1 immunostaining of PBS (E) , macrophages (F) , and FCs (G) immunized mice, respectively. Scale bars, 100 μm. (H) Quantification of MCP-1 expression in atherosclerotic lesion area of aortic root. n = 6 in each group; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Mouse TGF-β1 Precoated ELISA kit were provided by Boster Biological Technology Co., Ltd.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Immunostaining, Expressing

Journal: Oncology Letters

Article Title: Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer

doi: 10.3892/ol.2018.8510

Figure Lengend Snippet: Effects of CXCL13 on the concentrations of three cytokines (IL-1β, TNF and TGF-β1) were analyzed by ELISA in the Control, Model and Inhibitor groups. *P<0.05 vs. Control group; #P<0.05 vs. Model group. CXCL13, C-X-C motif chemokine ligand 13; IL-1β, interleukin-1β; TNF, tumor necrosis factor; TGF-β1, transforming growth factor β1.

Article Snippet: The concentrations of IL-1β (cat no. EK0394), TNF-α (cat no. EK0537) and tumor growth factor-β1 (TGF-β1) (cat no. EK0515) were detected using ELISA kits, according to the manufacturer's protocol (Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay