mouse specific ph2ax rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse specific ph2ax rabbit mab
    Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter <t>pH2AX</t> ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.
    Mouse Specific Ph2ax Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse specific ph2ax rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse specific ph2ax rabbit mab - by Bioz Stars, 2023-12
    86/100 stars

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    1) Product Images from "A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance"

    Article Title: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance

    Journal: Communications Chemistry

    doi: 10.1038/s42004-022-00661-z

    Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter pH2AX ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter pH2AX ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used: Activity Assay, Immunohistochemical staining, Staining, Software

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    Cell Signaling Technology Inc mouse specific ph2ax rabbit mab
    Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter <t>pH2AX</t> ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.
    Mouse Specific Ph2ax Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse specific ph2ax rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse specific ph2ax rabbit mab - by Bioz Stars, 2023-12
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter pH2AX ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Communications Chemistry

    Article Title: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance

    doi: 10.1038/s42004-022-00661-z

    Figure Lengend Snippet: Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter pH2AX ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Mouse-specific Ki-67 rabbit mAb (#12202) and mouse-specific pH2AX rabbit mAb (#9718) were purchased from Cell Signaling Technology (Beverly, MA, USA) and used following the manufacturer’s recommendations in a dilution of 1:200 (Ki-67) and 1:500 (pH2AX) for immunohistochemical staining.

    Techniques: Activity Assay, Immunohistochemical staining, Staining, Software