Journal: eLife

Article Title: Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function

doi: 10.7554/eLife.77968

Figure Lengend Snippet: ( A ) Volcano plot showing top ranking differentially expressed genes ( Ttr, Trh, Nrgn ) in hypothalamus of adult PPS-induced escalated aggressive males vs adult non-stressed control males ( B ) Volcano plot showing differentially expressed Ttr in opposite direction in hypothalamus of adult PPS-induced non-aggressive females vs adult non-stressed control females.

Article Snippet: The primary antibodies {anti-TTR rabbit polyclonal, anti-Nrgn rabbit polyclonal; anti-GAPDH mouse monoclonal} and secondary antibodies {anti-rabbit IgG HRP (Cell Signaling Technology, 7074P2) and anti-mouse IgG HRP (Cell Signaling Technology, 7076P2)} were used at adequate dilutions.

Techniques:

Journal: eLife

Article Title: Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function

doi: 10.7554/eLife.77968

Figure Lengend Snippet: ( A ) Experimental timeline for TTR expression analysis. Ttr mRNA expression profile in ( B ) Hypo and PFC of peripubertal stress exposed (PPS) adult male (SEagg) and female (SNAgg) mice with control (Ctrl), 24 hr after RI session and ( C ) Hypo and PFC of peripubertal male and female mice 24 hr after stress exposure (PPS) with control [no stress exposure (NS)] counterparts. (N=12 mice/biological replicates per group). Data are presented as mean (± SD) and analyzed by three-way ANOVA followed by Bonferroni’s post hoc test (**** p<0.0001). ( D ) Representative immunoblot of TTR protein levels with N=3 mice/biological replicates per group. Data are presented as mean (± SD) and analyzed by two-way ANOVA followed by Bonferroni’s post hoc test (**** p<0.0001, ***p=0.0001, *p<0.05). Immunofluorescence analysis (N=3 mice/ biological replicates per group) with left panels showing TTR immunoreactive cells (arrowheads) in Hypo ( E ) and PFC ( G ) and right panel showing TTR immunoreactivity in Hypo ( F ) and PFC ( H ) of Ctrl and SEagg mice. Figure 3—source data 1. Uncropped immunoblot of TTR and GAPDH.

Article Snippet: The primary antibodies {anti-TTR rabbit polyclonal, anti-Nrgn rabbit polyclonal; anti-GAPDH mouse monoclonal} and secondary antibodies {anti-rabbit IgG HRP (Cell Signaling Technology, 7074P2) and anti-mouse IgG HRP (Cell Signaling Technology, 7076P2)} were used at adequate dilutions.

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: eLife

Article Title: Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function

doi: 10.7554/eLife.77968

Figure Lengend Snippet: ( A ) Experimental timeline. T4 ( B ) and T3 ( C ) level in serum of peripubertal stress exposed (SEagg) and control (Ctrl) adult males 24 hr after RI session. T4 ( D ) and T3 ( E ) level in serum of peripubertal males 24 hr after stress exposure (PPS) with control [no stress exposure (NS)] counterparts. Data are presented as mean (± SD) and analyzed by unpaired Student’s t-test ns (p> 0.05). T4 ( F ) and T3 ( G ) level in Hypo and PFC of peripubertal stress exposed (SEagg) and control (Ctrl) adult males 24 hr after RI session. T4 ( H ) and T3 ( I ) level in Hypo and PFC of peripubertal males 24 hr after stress exposure (PPS) with control [no stress exposure (NS)] counterparts. All the above ELISA assays ‘B-I’ were performed in N=5 mice/biological replicates per group. Trh mRNA ( J ) and Nrgn mRNA ( K ) expression profile in Hypo and PFC of Ctrl and SEagg males (N=9 mice/biological replicates per group). NRGN protein expression ( L ) profile in Hypo and PFC of Ctrl and SEagg males and representative immunoblot with N=3 mice/biological replicates per group. Data are presented as mean (± SD) and analyzed by two-way ANOVA followed by Bonferroni’s post hoc test (**** p<0.0001, **p<0.01 and ns p>0.05). Figure 4—source data 1. Data points for T4 and T3 ELISA. Figure 4—source data 2. Uncropped immunoblot of NRGN and GAPDH protein.

Article Snippet: The primary antibodies {anti-TTR rabbit polyclonal, anti-Nrgn rabbit polyclonal; anti-GAPDH mouse monoclonal} and secondary antibodies {anti-rabbit IgG HRP (Cell Signaling Technology, 7074P2) and anti-mouse IgG HRP (Cell Signaling Technology, 7076P2)} were used at adequate dilutions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

Journal: eLife

Article Title: Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function

doi: 10.7554/eLife.77968

Figure Lengend Snippet: ( A ) Experimental strategy of stereotaxic surgeries followed by behavioral and molecular experiments ( B ) Comparative analysis of behavioural profile during RI session between hypothalamus injected Ttr esiRNA (N=16 mice/biological replicates per group) and scrambled siRNA (Scr) males (N=15 mice/biological replicates per group). ( C ) T3 content in hypothalamus (Hypo) of Ttr esiRNA (N=7 mice per group) and Scr males (N=6 mice/biological replicates per group). ( D ) Ttr mRNA, Nrgn mRNA, Hr mRNA and Trh mRNA expression analysis in Hypo of Ttr esiRNA and Scr males (N=9 mice/ biological replicates per group). Data are presented as mean (± SD) and analysed by unpaired Student’s t-test [*** (p< 0.001)] between Scr vs Ttr esiRNA group. Figure 5—source data 1. Data points for RI behavioral scoring. Figure 5—source data 2. Data points for T3 ELISA.

Article Snippet: The primary antibodies {anti-TTR rabbit polyclonal, anti-Nrgn rabbit polyclonal; anti-GAPDH mouse monoclonal} and secondary antibodies {anti-rabbit IgG HRP (Cell Signaling Technology, 7074P2) and anti-mouse IgG HRP (Cell Signaling Technology, 7076P2)} were used at adequate dilutions.

Techniques: Injection, esiRNA, Expressing, Enzyme-linked Immunosorbent Assay

Journal: eLife

Article Title: Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function

doi: 10.7554/eLife.77968

Figure Lengend Snippet:

Article Snippet: The primary antibodies {anti-TTR rabbit polyclonal, anti-Nrgn rabbit polyclonal; anti-GAPDH mouse monoclonal} and secondary antibodies {anti-rabbit IgG HRP (Cell Signaling Technology, 7074P2) and anti-mouse IgG HRP (Cell Signaling Technology, 7076P2)} were used at adequate dilutions.

Techniques: Methylated DNA Immunoprecipitation, Transfection, In Vivo, Enzyme-linked Immunosorbent Assay, Sequencing, esiRNA