Journal: PLOS Genetics

Article Title: Conserved NIMA kinases regulate multiple steps of endocytic trafficking

doi: 10.1371/journal.pgen.1010741

Figure Lengend Snippet: (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .

Article Snippet: Rabbit antibodies against NEK6 were obtained from Mybiosource (Cat# MBS94186), rabbit antibodies against NEK7 were obtained from Novus (Cat# 31110), mouse horseradish peroxidase (HRP)-conjugated antibodies against GAPDH were obtained from Proteintech (Cat# HRP-60004), rabbit antibodies against EEA1 were obtained from Cell Signaling (Cat# 3288), and mouse polyclonal antibodies against MICAL-L1 were obtained from Novus (Cat# H00085777-B01P).

Techniques: Western Blot, Transfection, Marker

Journal: Cell Reports Medicine

Article Title: A kinome-wide CRISPR screen identifies CK1α as a target to overcome enzalutamide resistance of prostate cancer

doi: 10.1016/j.xcrm.2023.101015

Figure Lengend Snippet:

Article Snippet: Goat polyclonal anti-mouse IgG, HRP-linked , Cell Signaling Technology , Cat# 7076; RRID: AB_330924.

Techniques: Magnetic Beads, Recombinant, Mutagenesis, Plasmid Preparation, CRISPR, Knock-Out, Software