mouse polyclonal antibodies against mical l1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse polyclonal antibodies against mical l1
    (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein <t>MICAL-L1.</t> (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .
    Mouse Polyclonal Antibodies Against Mical L1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal antibodies against mical l1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse polyclonal antibodies against mical l1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "Conserved NIMA kinases regulate multiple steps of endocytic trafficking"

    Article Title: Conserved NIMA kinases regulate multiple steps of endocytic trafficking

    Journal: PLOS Genetics

    doi: 10.1371/journal.pgen.1010741

    (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .
    Figure Legend Snippet: (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .

    Techniques Used: Western Blot, Transfection, Marker

    mouse polyclonal antibodies against mical l1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc mouse polyclonal antibodies against mical l1
    (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein <t>MICAL-L1.</t> (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm in B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in File 1.
    Mouse Polyclonal Antibodies Against Mical L1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal antibodies against mical l1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse polyclonal antibodies against mical l1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "Conserved NIMA kinases regulate multiple steps of endocytic trafficking"

    Article Title: Conserved NIMA kinases regulate multiple steps of endocytic trafficking

    Journal: bioRxiv

    doi: 10.1101/2022.12.22.521653

    (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm in B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in File 1.
    Figure Legend Snippet: (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm in B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in File 1.

    Techniques Used: Western Blot, Transfection, Marker

    Glioblastoma cells were mock transfected or were transfected with NEK6 siRNA or NEK7 siRNA. Transfected cells were then plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. Yellow box indicates region of enlarged inset. Scale bars = 10 μm.
    Figure Legend Snippet: Glioblastoma cells were mock transfected or were transfected with NEK6 siRNA or NEK7 siRNA. Transfected cells were then plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. Yellow box indicates region of enlarged inset. Scale bars = 10 μm.

    Techniques Used: Transfection, Marker

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    • mouse polyclonal antibodies against mical l1  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc mouse polyclonal antibodies against mical l1
    (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein <t>MICAL-L1.</t> (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .
    Mouse Polyclonal Antibodies Against Mical L1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal antibodies against mical l1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse polyclonal antibodies against mical l1 - by Bioz Stars, 2023-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .

    Journal: PLOS Genetics

    Article Title: Conserved NIMA kinases regulate multiple steps of endocytic trafficking

    doi: 10.1371/journal.pgen.1010741

    Figure Lengend Snippet: (A) siRNA knockdown of NEK6 (left panel) and NEK7 (right panel) was validated by western blotting of HeLa cells. (B–D) Mock-transfected cells (B), NEK6 siRNA–transfected cells (C), or NEK7 siRNA–transfected cells (D) were plated on coverslips and immunostained with antibodies against the tubular recycling endosome marker protein MICAL-L1. (E) The surface area of MICAL-L1–containing endosomes in mock-transfected, NEK6 siRNA–transfected, and NEK7 siRNA–transfected cells. Scale bar in D = 10 μm for B–D. Statistical significance was determined using Student’s unpaired t-test. Raw data are available in .

    Article Snippet: Rabbit antibodies against NEK6 were obtained from Mybiosource (Cat# MBS94186), rabbit antibodies against NEK7 were obtained from Novus (Cat# 31110), mouse horseradish peroxidase (HRP)-conjugated antibodies against GAPDH were obtained from Proteintech (Cat# HRP-60004), rabbit antibodies against EEA1 were obtained from Cell Signaling (Cat# 3288), and mouse polyclonal antibodies against MICAL-L1 were obtained from Novus (Cat# H00085777-B01P).

    Techniques: Western Blot, Transfection, Marker