Journal: Cell reports

Article Title: Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR

doi: 10.1016/j.celrep.2022.111092

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-S6 , Cell Signaling Technology , Cat# 2895; RRID: AB_2089254.

Techniques: Recombinant, Protease Inhibitor, Labeling, Transfection, Clone Assay, Staining, Multiplex Assay, Footprinting, Subcloning, Plasmid Preparation, shRNA, Software

Journal: American Journal of Physiology - Cell Physiology

Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

doi: 10.1152/ajpcell.00106.2016

Figure Lengend Snippet: List of TaqMan gene expression assays

Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

Techniques: Expressing

Journal: American Journal of Physiology - Cell Physiology

Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

doi: 10.1152/ajpcell.00106.2016

Figure Lengend Snippet: Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.

Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

Techniques: Expressing, Over Expression

Journal: American Journal of Physiology - Cell Physiology

Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

doi: 10.1152/ajpcell.00106.2016

Figure Lengend Snippet: The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.

Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

Techniques: Expressing

Journal: American Journal of Physiology - Cell Physiology

Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

doi: 10.1152/ajpcell.00106.2016

Figure Lengend Snippet: The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.

Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

Techniques: Expressing

Journal: American Journal of Physiology - Cell Physiology

Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

doi: 10.1152/ajpcell.00106.2016

Figure Lengend Snippet: The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.

Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

Techniques: Western Blot, Staining

Journal: American Journal of Physiology - Cell Physiology

Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

doi: 10.1152/ajpcell.00106.2016

Figure Lengend Snippet: In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.

Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

Techniques: In Vitro, Construct, Expressing