mouse polyclonal anti chop  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse polyclonal anti chop
    Mouse Polyclonal Anti Chop, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal anti chop/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    mouse polyclonal anti chop antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse polyclonal anti chop antibody
    Mouse Polyclonal Anti Chop Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal anti chop antibody/product/Cell Signaling Technology Inc
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    rabbit polyclonal anti s6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti s6

    Rabbit Polyclonal Anti S6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR"

    Article Title: Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR

    Journal: Cell reports

    doi: 10.1016/j.celrep.2022.111092


    Figure Legend Snippet:

    Techniques Used: Recombinant, Protease Inhibitor, Labeling, Transfection, Clone Assay, Staining, Multiplex Assay, Footprinting, Subcloning, Plasmid Preparation, shRNA, Software

    mouse polyclonal anti chop  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc mouse polyclonal anti chop
    Mouse Polyclonal Anti Chop, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal anti chop/product/Cell Signaling Technology Inc
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    polyclonal anti chop antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal anti chop antibody
    Polyclonal Anti Chop Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti chop antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
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    rabbit polyclonal antibody anti caspase 12  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody anti caspase 12
    Rabbit Polyclonal Antibody Anti Caspase 12, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody anti caspase 12/product/Cell Signaling Technology Inc
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    α 7 integrin cdb polyclonal rabbit anti rat antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc α 7 integrin cdb polyclonal rabbit anti rat antibody
    List of TaqMan gene expression assays
    α 7 Integrin Cdb Polyclonal Rabbit Anti Rat Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α 7 integrin cdb polyclonal rabbit anti rat antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
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    1) Product Images from "α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise"

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00106.2016

    List of TaqMan gene expression assays
    Figure Legend Snippet: List of TaqMan gene expression assays

    Techniques Used: Expressing

    Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.
    Figure Legend Snippet: Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.

    Techniques Used: Expressing, Over Expression

    The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.
    Figure Legend Snippet: The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.

    Techniques Used: Expressing

    The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.
    Figure Legend Snippet: The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.

    Techniques Used: Expressing

    The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.
    Figure Legend Snippet: The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.

    Techniques Used: Western Blot, Staining

    In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.
    Figure Legend Snippet: In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.

    Techniques Used: In Vitro, Construct, Expressing

    mouse polyclonal anti chop  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc mouse polyclonal anti chop
    Mouse Polyclonal Anti Chop, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse polyclonal anti chop/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse polyclonal anti chop - by Bioz Stars, 2024-06
    96/100 stars

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    α 7 integrin cdb polyclonal rabbit anti rat antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc α 7 integrin cdb polyclonal rabbit anti rat antibody
    List of TaqMan gene expression assays
    α 7 Integrin Cdb Polyclonal Rabbit Anti Rat Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α 7 integrin cdb polyclonal rabbit anti rat antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α 7 integrin cdb polyclonal rabbit anti rat antibody - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise"

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00106.2016

    List of TaqMan gene expression assays
    Figure Legend Snippet: List of TaqMan gene expression assays

    Techniques Used: Expressing

    Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.
    Figure Legend Snippet: Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.

    Techniques Used: Expressing, Over Expression

    The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.
    Figure Legend Snippet: The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.

    Techniques Used: Expressing

    The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.
    Figure Legend Snippet: The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.

    Techniques Used: Expressing

    The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.
    Figure Legend Snippet: The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.

    Techniques Used: Western Blot, Staining

    In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.
    Figure Legend Snippet: In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.

    Techniques Used: In Vitro, Construct, Expressing

    α 7 integrin cdb polyclonal rabbit anti rat antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc α 7 integrin cdb polyclonal rabbit anti rat antibody
    List of TaqMan gene expression assays
    α 7 Integrin Cdb Polyclonal Rabbit Anti Rat Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α 7 integrin cdb polyclonal rabbit anti rat antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α 7 integrin cdb polyclonal rabbit anti rat antibody - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise"

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00106.2016

    List of TaqMan gene expression assays
    Figure Legend Snippet: List of TaqMan gene expression assays

    Techniques Used: Expressing

    Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.
    Figure Legend Snippet: Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.

    Techniques Used: Expressing, Over Expression

    The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.
    Figure Legend Snippet: The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.

    Techniques Used: Expressing

    The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.
    Figure Legend Snippet: The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.

    Techniques Used: Expressing

    The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.
    Figure Legend Snippet: The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.

    Techniques Used: Western Blot, Staining

    In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.
    Figure Legend Snippet: In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.

    Techniques Used: In Vitro, Construct, Expressing

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    Cell Signaling Technology Inc mouse polyclonal anti chop
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    Image Search Results


    Journal: Cell reports

    Article Title: Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR

    doi: 10.1016/j.celrep.2022.111092

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-S6 , Cell Signaling Technology , Cat# 2895; RRID: AB_2089254.

    Techniques: Recombinant, Protease Inhibitor, Labeling, Transfection, Clone Assay, Staining, Multiplex Assay, Footprinting, Subcloning, Plasmid Preparation, shRNA, Software

    List of TaqMan gene expression assays

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    doi: 10.1152/ajpcell.00106.2016

    Figure Lengend Snippet: List of TaqMan gene expression assays

    Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

    Techniques: Expressing

    Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    doi: 10.1152/ajpcell.00106.2016

    Figure Lengend Snippet: Verification of transgene expression and impact of the α7 integrin on gene clustering after acute downhill running. A and B: sex differences were evident in rat (A) and mouse (B) α7β1 integrin gene expression. C: heat map of 274 genes with the most evidence for change within female groups. Red represents upregulation and blue represents downregulation. The four clusters are identified by color, including upregulation by the transgene (purple), downregulation by the transgene (black), upregulation by exercise (green), and downregulation by exercise (yellow). D and E: Venn diagrams representing differential response to exercise (D) and overexpression of the α7 integrin subunit (E) in female WT and α7Tg skeletal muscle. *Transgene main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to hypoxanthine phosphoribosyltransferase (Hprt). Data are expressed relative to WT-SED females.

    Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

    Techniques: Expressing, Over Expression

    The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    doi: 10.1152/ajpcell.00106.2016

    Figure Lengend Snippet: The α7 integrin regulates skeletal muscle gene expression. Rpl13a, Nosip, Ang, Slc7a5, Gys1, and Ndrg2 gene expression was examined in skeletal muscle (A–F). *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). All values are means ± SE, and all genes were normalized to Hprt and are expressed relative to WT-SED.

    Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

    Techniques: Expressing

    The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    doi: 10.1152/ajpcell.00106.2016

    Figure Lengend Snippet: The α7 integrin augments expression of genes necessary for protection from stress. Gene expression of Hsp40 (Dnajb9), Hspa5, and Atf6 combined (A, C, and E, respectively) and by sex (B, D, and F, respectively) was examined in skeletal muscle. *Transgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). †Sex main effect (P ≤ 0.05). All values are means ± SE and all genes were normalized to Hprt. Combined data are expressed relative to WT-SED and sex differences are expressed relative to WT-SED Female.

    Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

    Techniques: Expressing

    The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    doi: 10.1152/ajpcell.00106.2016

    Figure Lengend Snippet: The α7 integrin stimulates a beneficial UPR in skeletal muscle. A: representative immunoblots of unfolded protein response (UPR)-associated proteins. B–E: summary of data for total BiP (HSPA5) (B), phospho-IRE-1α (C), phospho-eIF2α (D), and total CHOP (E). *Transgene main effect (P ≤ 0.05). All values are means ± SE and all proteins were normalized to Ponceau S staining and are expressed relative to WT-SED. M, male; F, female.

    Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

    Techniques: Western Blot, Staining

    In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: α 7 β 1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise

    doi: 10.1152/ajpcell.00106.2016

    Figure Lengend Snippet: In vitro evaluation of α7 integrin-mediated gene transcription. A: validation of two constructs that allow for α7 integrin knockdown in myotubes. B: SLC7A5 protein expression was examined in skeletal muscle. C and D: evaluation of target gene transcription with α7 integrin knockdown. *Ttransgene main effect (P ≤ 0.05). ‡Exercise main effect (P ≤ 0.05). **Different from scramble control (P ≤ 0.05). All values are means ± SE, and all genes are normalized to Hprt and are expressed relative to scramble control.

    Article Snippet: The following antibodies were used: α 7 integrin CDB polyclonal rabbit anti-rat antibody, CHOP (L63F7) (2895S; Cell Signaling), GRP78/HSPA5/BiP (ADI-SPA-826-D; Enzo Life Sciences), IRE1α (Ser724) (NB100–2323; Novus Biologicals), eIF2α (Ser51) (119A11) (3597S; Cell Signaling), and SLC7A5 (bs-10125R; Bioss Antibodies).

    Techniques: In Vitro, Construct, Expressing