mouse plasma fibronectin  (Thermo Fisher)


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  • 93
    Name:
    Fibronectin Ab 1 Mouse Monoclonal Antibody
    Description:

    Catalog Number:
    MS-165-P
    Price:
    None
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    Structured Review

    Thermo Fisher mouse plasma fibronectin

    https://www.bioz.com/result/mouse plasma fibronectin/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse plasma fibronectin - by Bioz Stars, 2021-04
    93/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Cooperative behaviour and phenotype plasticity evolve during melanoma progression, et al. Cooperative behaviour and phenotype plasticity evolve during melanoma progression
    Article Snippet: Intriguingly, the expression of human fibronectin (FN1) was also increased (Figure ), suggesting that it is also favourable for tumour growth. .. Immunohistochemistry (IHC) analysis using a fibronectin antibody with reactivity to mouse and human revealed a similar staining intensity in 501mel and WM266‐4 tumours despite a significant lower mRNA expression of human FN1 in 501mel tumours (Figure ). .. However, murine Fn1 expression was elevated in 501mel tumours (Figure ), and this correlated with an abundance of cells positive for the cancer‐associated fibroblast (CAF) marker αSMA (Figure ).

    Staining:

    Article Title: Cooperative behaviour and phenotype plasticity evolve during melanoma progression, et al. Cooperative behaviour and phenotype plasticity evolve during melanoma progression
    Article Snippet: Intriguingly, the expression of human fibronectin (FN1) was also increased (Figure ), suggesting that it is also favourable for tumour growth. .. Immunohistochemistry (IHC) analysis using a fibronectin antibody with reactivity to mouse and human revealed a similar staining intensity in 501mel and WM266‐4 tumours despite a significant lower mRNA expression of human FN1 in 501mel tumours (Figure ). .. However, murine Fn1 expression was elevated in 501mel tumours (Figure ), and this correlated with an abundance of cells positive for the cancer‐associated fibroblast (CAF) marker αSMA (Figure ).

    Expressing:

    Article Title: Cooperative behaviour and phenotype plasticity evolve during melanoma progression, et al. Cooperative behaviour and phenotype plasticity evolve during melanoma progression
    Article Snippet: Intriguingly, the expression of human fibronectin (FN1) was also increased (Figure ), suggesting that it is also favourable for tumour growth. .. Immunohistochemistry (IHC) analysis using a fibronectin antibody with reactivity to mouse and human revealed a similar staining intensity in 501mel and WM266‐4 tumours despite a significant lower mRNA expression of human FN1 in 501mel tumours (Figure ). .. However, murine Fn1 expression was elevated in 501mel tumours (Figure ), and this correlated with an abundance of cells positive for the cancer‐associated fibroblast (CAF) marker αSMA (Figure ).

    Blocking Assay:

    Article Title: Large Gradient High Magnetic Fields Affect Osteoblast Ultrastructure and Function by Disrupting Collagen I or Fibronectin/??1 Integrin
    Article Snippet: In Cell Western An ICW assay was performed according to Odyssey Infrared Imaging System application protocols , . .. After 24 h of culturing, MG-63 and MC3T3-E1 cells were washed and fixed in 4% paraformaldehyde in PBS for 30 min at room temperature, then the cells were permeabilized in 0.1% Triton X-100 for 5 min. After 2 h of blocking with blocking buffer (1% BSA, 5% sucrose, and 0.05% NaN3 in PBS), cells were incubated in the following first antibodies in PBS overnight at 4°C: rabbit polyclonal integrin α3 (H-43) and integrin α5 (H-104) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); mouse anti-integrin αv (BD Pharmingen, San Diego, CA, USA); mouse monoclonal anti-integrin β1 (Calbiochem, San Diego, CA, USA); rabbit polyclonal anti-collagen I (Abcam, Cambridge, UK); and mouse monoclonal anti-fibronectin (Thermo Fisher Scientific, San Jose , CA, USA). .. The specificity of these antibodies was tested using Western blot prior to ICW assay.

    Article Title: Transcriptome Analysis of Epithelial and Stromal Contributions to Mammogenesis in Three Week Prepartum Cows
    Article Snippet: .. Sections were then incubated for 10 min at RT in non-immune blocking serum block (Histostain kit; Zymed Laboratories, San Francisco, CA), excess blocking serum was blotted off and sections were incubated with the primary mouse monoclonal anti-fibronectin (50 µg/ml; 1 hr; MS-1351-R7; Lab Vision Products, Fremont, CA) or mouse polyclonal anti-MMP-3 (1∶200; 45 min; 3523R-100; Biovision Inc., Mountain View, CA) at RT diluted in blocking solution. .. Sections were washed in PBS and incubated with a broad-spectrum secondary antibody conjugated with streptavidin-peroxidase (Histostain kit; Zymed Laboratories Inc.), followed by a PBS wash and 3 min incubation with 3′, 3′-diaminobenzidine chromogen substrate.

    Incubation:

    Article Title: Large Gradient High Magnetic Fields Affect Osteoblast Ultrastructure and Function by Disrupting Collagen I or Fibronectin/??1 Integrin
    Article Snippet: In Cell Western An ICW assay was performed according to Odyssey Infrared Imaging System application protocols , . .. After 24 h of culturing, MG-63 and MC3T3-E1 cells were washed and fixed in 4% paraformaldehyde in PBS for 30 min at room temperature, then the cells were permeabilized in 0.1% Triton X-100 for 5 min. After 2 h of blocking with blocking buffer (1% BSA, 5% sucrose, and 0.05% NaN3 in PBS), cells were incubated in the following first antibodies in PBS overnight at 4°C: rabbit polyclonal integrin α3 (H-43) and integrin α5 (H-104) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); mouse anti-integrin αv (BD Pharmingen, San Diego, CA, USA); mouse monoclonal anti-integrin β1 (Calbiochem, San Diego, CA, USA); rabbit polyclonal anti-collagen I (Abcam, Cambridge, UK); and mouse monoclonal anti-fibronectin (Thermo Fisher Scientific, San Jose , CA, USA). .. The specificity of these antibodies was tested using Western blot prior to ICW assay.

    Article Title: Transcriptome Analysis of Epithelial and Stromal Contributions to Mammogenesis in Three Week Prepartum Cows
    Article Snippet: .. Sections were then incubated for 10 min at RT in non-immune blocking serum block (Histostain kit; Zymed Laboratories, San Francisco, CA), excess blocking serum was blotted off and sections were incubated with the primary mouse monoclonal anti-fibronectin (50 µg/ml; 1 hr; MS-1351-R7; Lab Vision Products, Fremont, CA) or mouse polyclonal anti-MMP-3 (1∶200; 45 min; 3523R-100; Biovision Inc., Mountain View, CA) at RT diluted in blocking solution. .. Sections were washed in PBS and incubated with a broad-spectrum secondary antibody conjugated with streptavidin-peroxidase (Histostain kit; Zymed Laboratories Inc.), followed by a PBS wash and 3 min incubation with 3′, 3′-diaminobenzidine chromogen substrate.

    Purification:

    Article Title: Interactions between Germ Cells and Extracellular Matrix Glycoproteins during Migration and Gonad Assembly in the Mouse Embryo
    Article Snippet: .. FN antibody was also a rabbit IgG raised against purified mouse plasma fibronectin ( GIBCO BRL ). ..

    Mass Spectrometry:

    Article Title: Transcriptome Analysis of Epithelial and Stromal Contributions to Mammogenesis in Three Week Prepartum Cows
    Article Snippet: .. Sections were then incubated for 10 min at RT in non-immune blocking serum block (Histostain kit; Zymed Laboratories, San Francisco, CA), excess blocking serum was blotted off and sections were incubated with the primary mouse monoclonal anti-fibronectin (50 µg/ml; 1 hr; MS-1351-R7; Lab Vision Products, Fremont, CA) or mouse polyclonal anti-MMP-3 (1∶200; 45 min; 3523R-100; Biovision Inc., Mountain View, CA) at RT diluted in blocking solution. .. Sections were washed in PBS and incubated with a broad-spectrum secondary antibody conjugated with streptavidin-peroxidase (Histostain kit; Zymed Laboratories Inc.), followed by a PBS wash and 3 min incubation with 3′, 3′-diaminobenzidine chromogen substrate.

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    Thermo Fisher mouse plasma fibronectin
    Adhesion and migration of PS explant cells in Boyden chambers. PS explants from α4-positive (A–D) or -null (E–F) embryos were placed on membranes coated with VCAM-1 (A and B) or <t>fibronectin</t> (C–F). After overnight incubation, each explant was photographed (A, C, and E) and removed from the top side; the bottom side was then photographed (B, D, and F). Note the nuclei of explant cells that adhered to VCAM-1 and fibronectin (A, C, and E) and migrated through pores (B and D, arrows). α4-null explant cells failed to migrate through pores (F, arrow). FN, fibronectin. Bar, 0.1 mm.
    Mouse Plasma Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse plasma fibronectin/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
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    mouse plasma fibronectin - by Bioz Stars, 2021-04
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    95
    Thermo Fisher fibronectin
    Migration of murine dermal fibroblasts after 20 hours of culture. Coverslips coated with colloidal gold salts and extracellular matrix molecules after fibroblast incubation viewed with ×40 magnification under dark-field optics. A: Wild-type murine dermal fibroblasts plated on 20 μg/ml of <t>fibronectin.</t> B: Diabetic db / db murine dermal fibroblasts plated on 20 μg/ml of fibronectin. The migration tracks ( asterisk ) are visible as black empty spaces against the background of bright gold salt particles. The cells are indicated by arrows . Note how the migration tracks of the wild-type cells are clearly larger than those of the diabetic cells.
    Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher human plasma fibronectin
    Breast tumor ECM mimetic stiffness array. (a) 96 well PA hydrogel stiffness arrays encumbering the mechanical profile of primary tumor ECM. Here, stiffness is systematically increased across the plate, with untreated glass included as a rigid control (moduli ≈10 5 MPa). To prepare the multi-well array, glass bottom plates are chemically treated (red) to covalently attach PA gels formed in each well (purple). Functionalization of the gel surface with <t>fibronectin</t> (orange) allows for attachment of seeded cells. (b) Stiffness array gels were prepared with elastic moduli values that matched the rigidity profile of primary mammary tumor ECM (5 to 60 kPa) by controlling the weight percent of bis-acrylamide (bis) crosslinker added to the polymer solution (n = 3). (c) Representative immnofluorescent image demonstrating homogenous display of covalently linked fibronectin (red) on the gel surface (scale bar = 400 m). Associated z-stacks confirm protein coating is limited to the top of the gels. (d) Proliferation of primary mammary tumor cells isolated from MMTV-PYMT mice on 5 to 30 kPa PA gels, as measured by cell nuclei counting (n = 3). (e) Cell spreading as a function of substrate stiffness for primary tumor cells (1° tumor) or the human breast cancer cell li ne MCF-7 (n = 30). Error bars represent one standard deviation
    Human Plasma Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human plasma fibronectin/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human plasma fibronectin - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Adhesion and migration of PS explant cells in Boyden chambers. PS explants from α4-positive (A–D) or -null (E–F) embryos were placed on membranes coated with VCAM-1 (A and B) or fibronectin (C–F). After overnight incubation, each explant was photographed (A, C, and E) and removed from the top side; the bottom side was then photographed (B, D, and F). Note the nuclei of explant cells that adhered to VCAM-1 and fibronectin (A, C, and E) and migrated through pores (B and D, arrows). α4-null explant cells failed to migrate through pores (F, arrow). FN, fibronectin. Bar, 0.1 mm.

    Journal: The Journal of Cell Biology

    Article Title: Dual functions of ?4?1 integrin in epicardial development

    doi: 10.1083/jcb.200203075

    Figure Lengend Snippet: Adhesion and migration of PS explant cells in Boyden chambers. PS explants from α4-positive (A–D) or -null (E–F) embryos were placed on membranes coated with VCAM-1 (A and B) or fibronectin (C–F). After overnight incubation, each explant was photographed (A, C, and E) and removed from the top side; the bottom side was then photographed (B, D, and F). Note the nuclei of explant cells that adhered to VCAM-1 and fibronectin (A, C, and E) and migrated through pores (B and D, arrows). α4-null explant cells failed to migrate through pores (F, arrow). FN, fibronectin. Bar, 0.1 mm.

    Article Snippet: 10 μg/ml mouse plasma fibronectin (Life Technologies) or soluble VCAM-1, provided by Roy Lobb (Biogene, Cambridge, MA) in DME were added to the bottom compartments of the chambers.

    Techniques: Migration, Incubation

    Fibronectin and VCAM-1 localization by immunostaining. Embryo sections were stained with an anti-fibronectin (A and C) or an anti–VCAM-1 antibody (B and D). C and D are higher magnifications of A and B, respectively, showing the PS region. Note that fibronectin is localized in the periphery of cysts that are budding out (arrowhead) and in the myocardium. VCAM-1 is localized in the myocardium. a, atrium; v, ventricle; my, myocardium. Bars, 0.1 mm.

    Journal: The Journal of Cell Biology

    Article Title: Dual functions of ?4?1 integrin in epicardial development

    doi: 10.1083/jcb.200203075

    Figure Lengend Snippet: Fibronectin and VCAM-1 localization by immunostaining. Embryo sections were stained with an anti-fibronectin (A and C) or an anti–VCAM-1 antibody (B and D). C and D are higher magnifications of A and B, respectively, showing the PS region. Note that fibronectin is localized in the periphery of cysts that are budding out (arrowhead) and in the myocardium. VCAM-1 is localized in the myocardium. a, atrium; v, ventricle; my, myocardium. Bars, 0.1 mm.

    Article Snippet: 10 μg/ml mouse plasma fibronectin (Life Technologies) or soluble VCAM-1, provided by Roy Lobb (Biogene, Cambridge, MA) in DME were added to the bottom compartments of the chambers.

    Techniques: Immunostaining, Staining

    Migration of murine dermal fibroblasts after 20 hours of culture. Coverslips coated with colloidal gold salts and extracellular matrix molecules after fibroblast incubation viewed with ×40 magnification under dark-field optics. A: Wild-type murine dermal fibroblasts plated on 20 μg/ml of fibronectin. B: Diabetic db / db murine dermal fibroblasts plated on 20 μg/ml of fibronectin. The migration tracks ( asterisk ) are visible as black empty spaces against the background of bright gold salt particles. The cells are indicated by arrows . Note how the migration tracks of the wild-type cells are clearly larger than those of the diabetic cells.

    Journal: The American Journal of Pathology

    Article Title: Cellular Dysfunction in the Diabetic Fibroblast

    doi:

    Figure Lengend Snippet: Migration of murine dermal fibroblasts after 20 hours of culture. Coverslips coated with colloidal gold salts and extracellular matrix molecules after fibroblast incubation viewed with ×40 magnification under dark-field optics. A: Wild-type murine dermal fibroblasts plated on 20 μg/ml of fibronectin. B: Diabetic db / db murine dermal fibroblasts plated on 20 μg/ml of fibronectin. The migration tracks ( asterisk ) are visible as black empty spaces against the background of bright gold salt particles. The cells are indicated by arrows . Note how the migration tracks of the wild-type cells are clearly larger than those of the diabetic cells.

    Article Snippet: Dulbecco’s modified Eagle’s medium, fetal bovine serum (FBS), trypsin, collagenase I, fibronectin, phosphate-buffered saline (PBS), Hanks’ balanced salt solution, and antibiotics for cell culture were all obtained from Life Technologies Inc. (Grand Island, NY).

    Techniques: Migration, Incubation

    Haptotaxis assays confirm impaired migration of diabetic fibroblasts on collagen and fibronectin. Modified Boyden chamber haptotaxis assays were prepared as described in Materials and Methods. A: Haptotaxis on collagen- and fibronectin-coated inserts. B: Up-regulation of haptotaxis by hypoxia (1% O 2 ) stimulation on collagen-coated dishes. *, P

    Journal: The American Journal of Pathology

    Article Title: Cellular Dysfunction in the Diabetic Fibroblast

    doi:

    Figure Lengend Snippet: Haptotaxis assays confirm impaired migration of diabetic fibroblasts on collagen and fibronectin. Modified Boyden chamber haptotaxis assays were prepared as described in Materials and Methods. A: Haptotaxis on collagen- and fibronectin-coated inserts. B: Up-regulation of haptotaxis by hypoxia (1% O 2 ) stimulation on collagen-coated dishes. *, P

    Article Snippet: Dulbecco’s modified Eagle’s medium, fetal bovine serum (FBS), trypsin, collagenase I, fibronectin, phosphate-buffered saline (PBS), Hanks’ balanced salt solution, and antibiotics for cell culture were all obtained from Life Technologies Inc. (Grand Island, NY).

    Techniques: Migration, Modification

    Diabetic fibroblasts show impaired migration on collagen and fibronectin compared to wild-type fibroblasts. Fibroblast migration assays were performed as described in Materials and Methods. In the gold-salt phagokinetic migration assay, fibroblasts were plated onto coverslips coated with varying concentrations of the matrix of interest and allowed to migrate for 20 hours. The figures depict mean migration track area ± SEM of fibroblasts from diabetic db / db and wild-type mice. Area is shown in pixels. A: Fibroblast migration on type I collagen. B: Fibroblast migration on fibronectin. *, P

    Journal: The American Journal of Pathology

    Article Title: Cellular Dysfunction in the Diabetic Fibroblast

    doi:

    Figure Lengend Snippet: Diabetic fibroblasts show impaired migration on collagen and fibronectin compared to wild-type fibroblasts. Fibroblast migration assays were performed as described in Materials and Methods. In the gold-salt phagokinetic migration assay, fibroblasts were plated onto coverslips coated with varying concentrations of the matrix of interest and allowed to migrate for 20 hours. The figures depict mean migration track area ± SEM of fibroblasts from diabetic db / db and wild-type mice. Area is shown in pixels. A: Fibroblast migration on type I collagen. B: Fibroblast migration on fibronectin. *, P

    Article Snippet: Dulbecco’s modified Eagle’s medium, fetal bovine serum (FBS), trypsin, collagenase I, fibronectin, phosphate-buffered saline (PBS), Hanks’ balanced salt solution, and antibiotics for cell culture were all obtained from Life Technologies Inc. (Grand Island, NY).

    Techniques: Migration, Mouse Assay

    Loss of hypoxia-inducible factor 1α from macrophages impairs invasion capabilities under hypoxic conditions. Sections of spleen tissue from naïve and Friend virus (FV)-infected mice (7 days after infection [dpi]) were stained with 3,3′- diaminobenzidine (DAB) pimonidazole (brown) and then counterstained with haematoxylin ( a , blue). Spleens of naïve and FV-infected mice (7 dpi) were removed, and proteins were directly isolated in a hypoxic workstation under hypoxic conditions (1% O 2 ) so that rapid degradation of hypoxia-inducible factor (HIF) protein could be avoided. One part of the spleen was removed from the workstation and exposed to atmospheric conditions (20.9% O 2 ) for 10 minutes for reoxygenation before protein isolation. Subsequently, Western blot analysis was performed with specific antibodies for HIF-1α and ACTIN ( b ). An inverted invasion assay was performed to compare 3D invasion of bone marrow–derived macrophages (BMDMs) from naïve wild-type (WT) and HIF-1α knockout (KO) mice under normoxic and hypoxic conditions. BMDMs were allowed to invade a Matrigel/fibronectin gel for 72 hours. Subsequently, cells were stained with calcein acetoxymethyl (AM) and visualised by confocal microscopy ( c ; original magnification, 200×). Three randomly chosen fields per well were recorded, and the number of invaded BMDMs was determined. For viability testing, dead BMDMs were stained with DAPI, and stained cells were counted manually in relation to the total number of cells in the field. Positive controls were treated with 0.1% Triton X-100. ( d ). Data were analysed with Student’s t-test (mean + SEM). n = 3–4. *P = 0.05.

    Journal: Scientific Reports

    Article Title: Hypoxia-inducible factor 1α is Essential for Macrophage-mediated Erythroblast Proliferation in Acute Friend Retrovirus Infection

    doi: 10.1038/s41598-017-17324-y

    Figure Lengend Snippet: Loss of hypoxia-inducible factor 1α from macrophages impairs invasion capabilities under hypoxic conditions. Sections of spleen tissue from naïve and Friend virus (FV)-infected mice (7 days after infection [dpi]) were stained with 3,3′- diaminobenzidine (DAB) pimonidazole (brown) and then counterstained with haematoxylin ( a , blue). Spleens of naïve and FV-infected mice (7 dpi) were removed, and proteins were directly isolated in a hypoxic workstation under hypoxic conditions (1% O 2 ) so that rapid degradation of hypoxia-inducible factor (HIF) protein could be avoided. One part of the spleen was removed from the workstation and exposed to atmospheric conditions (20.9% O 2 ) for 10 minutes for reoxygenation before protein isolation. Subsequently, Western blot analysis was performed with specific antibodies for HIF-1α and ACTIN ( b ). An inverted invasion assay was performed to compare 3D invasion of bone marrow–derived macrophages (BMDMs) from naïve wild-type (WT) and HIF-1α knockout (KO) mice under normoxic and hypoxic conditions. BMDMs were allowed to invade a Matrigel/fibronectin gel for 72 hours. Subsequently, cells were stained with calcein acetoxymethyl (AM) and visualised by confocal microscopy ( c ; original magnification, 200×). Three randomly chosen fields per well were recorded, and the number of invaded BMDMs was determined. For viability testing, dead BMDMs were stained with DAPI, and stained cells were counted manually in relation to the total number of cells in the field. Positive controls were treated with 0.1% Triton X-100. ( d ). Data were analysed with Student’s t-test (mean + SEM). n = 3–4. *P = 0.05.

    Article Snippet: Briefly, 100 mL of Matrigel (BD Biosciences), mixed 1:1 with ice-cold PBSand supplemented with 50 mg/mL bovine plasma fibronectin (Invitrogen), was transferred to a transwellinsert (8-mm pore; Corning Incorporated, Corning, NY, USA) to polymerise at 37 °C.

    Techniques: Infection, Mouse Assay, Staining, Isolation, Western Blot, Invasion Assay, Derivative Assay, Knock-Out, Confocal Microscopy

    Breast tumor ECM mimetic stiffness array. (a) 96 well PA hydrogel stiffness arrays encumbering the mechanical profile of primary tumor ECM. Here, stiffness is systematically increased across the plate, with untreated glass included as a rigid control (moduli ≈10 5 MPa). To prepare the multi-well array, glass bottom plates are chemically treated (red) to covalently attach PA gels formed in each well (purple). Functionalization of the gel surface with fibronectin (orange) allows for attachment of seeded cells. (b) Stiffness array gels were prepared with elastic moduli values that matched the rigidity profile of primary mammary tumor ECM (5 to 60 kPa) by controlling the weight percent of bis-acrylamide (bis) crosslinker added to the polymer solution (n = 3). (c) Representative immnofluorescent image demonstrating homogenous display of covalently linked fibronectin (red) on the gel surface (scale bar = 400 m). Associated z-stacks confirm protein coating is limited to the top of the gels. (d) Proliferation of primary mammary tumor cells isolated from MMTV-PYMT mice on 5 to 30 kPa PA gels, as measured by cell nuclei counting (n = 3). (e) Cell spreading as a function of substrate stiffness for primary tumor cells (1° tumor) or the human breast cancer cell li ne MCF-7 (n = 30). Error bars represent one standard deviation

    Journal: Biomaterials

    Article Title: Identification of a Mechanogenetic Link between Substrate Stiffness and Chemotherapeutic Response in Breast Cancer

    doi: 10.1016/j.biomaterials.2019.02.018

    Figure Lengend Snippet: Breast tumor ECM mimetic stiffness array. (a) 96 well PA hydrogel stiffness arrays encumbering the mechanical profile of primary tumor ECM. Here, stiffness is systematically increased across the plate, with untreated glass included as a rigid control (moduli ≈10 5 MPa). To prepare the multi-well array, glass bottom plates are chemically treated (red) to covalently attach PA gels formed in each well (purple). Functionalization of the gel surface with fibronectin (orange) allows for attachment of seeded cells. (b) Stiffness array gels were prepared with elastic moduli values that matched the rigidity profile of primary mammary tumor ECM (5 to 60 kPa) by controlling the weight percent of bis-acrylamide (bis) crosslinker added to the polymer solution (n = 3). (c) Representative immnofluorescent image demonstrating homogenous display of covalently linked fibronectin (red) on the gel surface (scale bar = 400 m). Associated z-stacks confirm protein coating is limited to the top of the gels. (d) Proliferation of primary mammary tumor cells isolated from MMTV-PYMT mice on 5 to 30 kPa PA gels, as measured by cell nuclei counting (n = 3). (e) Cell spreading as a function of substrate stiffness for primary tumor cells (1° tumor) or the human breast cancer cell li ne MCF-7 (n = 30). Error bars represent one standard deviation

    Article Snippet: Human plasma fibronectin and tetramethylrhodamine conjugated streptavidin were purchased from Life Technologies.

    Techniques: Isolation, Mouse Assay, Standard Deviation