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Agilent technologies mouse pgc 1α cdna
Reduced number of main arteries and veins in <t>PGC-1α</t> −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
Mouse Pgc 1α Cdna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina"

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

Journal: The American Journal of Pathology

doi: 10.1016/j.ajpath.2012.09.003

Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
Figure Legend Snippet: Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Knock-Out

PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P
Figure Legend Snippet: PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

Techniques Used: Pyrolysis Gas Chromatography, Infection, Expressing, Negative Control, Isolation, Real-time Polymerase Chain Reaction, shRNA

PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P
Figure Legend Snippet: PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Staining, Software

Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P
Figure Legend Snippet: Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Staining

Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P
Figure Legend Snippet: Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

Techniques Used: Pyrolysis Gas Chromatography, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Laser Capture Microdissection

PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P
Figure Legend Snippet: PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

Techniques Used: Pyrolysis Gas Chromatography, Expressing, Quantitative RT-PCR, Mouse Assay

Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.
Figure Legend Snippet: Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Staining

Related Articles

Plasmid Preparation:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

Subcloning:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

Pyrolysis Gas Chromatography:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

Labeling:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

In Vitro:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

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    Agilent technologies mouse pgc 1α cdna
    Reduced number of main arteries and veins in <t>PGC-1α</t> −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
    Mouse Pgc 1α Cdna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse pgc 1α cdna/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse pgc 1α cdna - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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    Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Knock-Out

    PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Infection, Expressing, Negative Control, Isolation, Real-time Polymerase Chain Reaction, shRNA

    PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining, Software

    Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining

    Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Laser Capture Microdissection

    PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Quantitative RT-PCR, Mouse Assay

    Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining