mouse notch1 gene cdna  (ATCC)


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    Structured Review

    ATCC mouse notch1 gene cdna
    Primary and secondary antibodies used in Western blot analysis.
    Mouse Notch1 Gene Cdna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse notch1 gene cdna/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse notch1 gene cdna - by Bioz Stars, 2024-03
    93/100 stars

    Images

    1) Product Images from "NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells"

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    Journal: Cells

    doi: 10.3390/cells9092032

    Primary and secondary antibodies used in Western blot analysis.
    Figure Legend Snippet: Primary and secondary antibodies used in Western blot analysis.

    Techniques Used: Western Blot

    NOTCH signaling in mesenchymal C3H10T1/2 cells stably overexpressing NOTCH receptors or the HES1 protein. ( A ) NOTCH receptors’ transcriptional activity, as measured by luciferase assays, in each of the four stably Notch -transfected pools. The relative luciferase activities were always normalized to renilla levels. ( B ) qRT-PCR analysis of the relative Hes1 and Hey1 mRNA transcription levels in each of the stably Notch -transfected pools. ( C ) qRT-PCR analysis of relative mRNA transcription levels of Notch genes in the stable Notch1 transfectant (N1S), the stable Notch2 transfectant (N2S), the stable Notch3 transfectant (N3S), and the stable Notch4 transfectant (N4S). ( D ) qRT-PCR analysis of Dlk1 (DELTA-like 1 homolog) and Dlk2 (DELTA-like 2 homolog) mRNA transcription levels in the stable Notch transfectants. ( E ) qRT-PCR analysis of the relative Hes1 and Dlk mRNA transcription levels in the stable Hes1 gene transfectant (H1S). All these assays were performed using non-differentiated cells. Data in qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition in all assays was measured relative to levels in non-differentiated empty-vector-transfected cells, set arbitrarily at 1 (horizontal black line or V). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance of Student’s t -test results is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: NOTCH signaling in mesenchymal C3H10T1/2 cells stably overexpressing NOTCH receptors or the HES1 protein. ( A ) NOTCH receptors’ transcriptional activity, as measured by luciferase assays, in each of the four stably Notch -transfected pools. The relative luciferase activities were always normalized to renilla levels. ( B ) qRT-PCR analysis of the relative Hes1 and Hey1 mRNA transcription levels in each of the stably Notch -transfected pools. ( C ) qRT-PCR analysis of relative mRNA transcription levels of Notch genes in the stable Notch1 transfectant (N1S), the stable Notch2 transfectant (N2S), the stable Notch3 transfectant (N3S), and the stable Notch4 transfectant (N4S). ( D ) qRT-PCR analysis of Dlk1 (DELTA-like 1 homolog) and Dlk2 (DELTA-like 2 homolog) mRNA transcription levels in the stable Notch transfectants. ( E ) qRT-PCR analysis of the relative Hes1 and Dlk mRNA transcription levels in the stable Hes1 gene transfectant (H1S). All these assays were performed using non-differentiated cells. Data in qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition in all assays was measured relative to levels in non-differentiated empty-vector-transfected cells, set arbitrarily at 1 (horizontal black line or V). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance of Student’s t -test results is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Techniques Used: Stable Transfection, Activity Assay, Luciferase, Transfection, Quantitative RT-PCR, Activation Assay, Inhibition, Plasmid Preparation

    Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells enhanced adipogenesis levels. Representative microscopic images of adipocytes from C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ) showing their adipogenic levels (50× magnification images, scale bar 200 μm) after oil red O staining, all compared with the adipogenic levels of empty-vector transfected cells (VD). D: differentiated cells. qRT-PCR analysis of indicated adipogenic marker mRNA transcription levels in the differentiated stable Notch1 -overexpressing cells (N1SD) ( C ), the differentiated stable Notch2- overexpressing cells (N2SD) ( D ), the differentiated stable Notch3 -overexpressing cells (N3SD) ( E ), the differentiated stable Notch4- overexpressing cells (N4SD) ( F ), the differentiated stable Dlk1 -overexpressing cells (DLK1SD) ( G ), and the differentiated stable Dlk2 -overexpressing cells (DLK2SD) ( H ). Data from all qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in qRT-PCR assays were calculated relative to the levels shown by seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells enhanced adipogenesis levels. Representative microscopic images of adipocytes from C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ) showing their adipogenic levels (50× magnification images, scale bar 200 μm) after oil red O staining, all compared with the adipogenic levels of empty-vector transfected cells (VD). D: differentiated cells. qRT-PCR analysis of indicated adipogenic marker mRNA transcription levels in the differentiated stable Notch1 -overexpressing cells (N1SD) ( C ), the differentiated stable Notch2- overexpressing cells (N2SD) ( D ), the differentiated stable Notch3 -overexpressing cells (N3SD) ( E ), the differentiated stable Notch4- overexpressing cells (N4SD) ( F ), the differentiated stable Dlk1 -overexpressing cells (DLK1SD) ( G ), and the differentiated stable Dlk2 -overexpressing cells (DLK2SD) ( H ). Data from all qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in qRT-PCR assays were calculated relative to the levels shown by seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Techniques Used: Over Expression, Transfection, Staining, Plasmid Preparation, Quantitative RT-PCR, Marker, Activation Assay, Inhibition

    Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells modulated brown adipogenesis. ( A ) Representative microscopic images of adipocytes of C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ), showing the size of their lipid droplets (400× magnification images, scale bar 30 μm) after oil red O staining, all of them compared with the size of lipid droplets of empty-vector-transfected differentiated cells (VD). qRT-PCR analysis of the indicated brown marker expression levels in the differentiated stably Notch1 -transfected cells (N1SD) ( C ), the differentiated stably Notch2- transfected cells (N2SD) ( D ), the differentiated stably Notch3- transfected cells (N3SD) ( E ), the differentiated stably Notch4- transfected cells (N4SD) ( F ), the differentiated stably Dlk1 -transfected cells (DLK1SD) ( H ) and the differentiated stably Dlk2 -transfected cells (DLK2SD) ( I ). ( G ) qPCR analysis of mtCytb (mitochondrial cytochrome b) DNA amplification (related to genomic ApoB DNA amplification; see Materials and Methods section) in seven-day differentiated C3H10T1/2 cells overexpressing Notch ( G ) and Dlk ( J ) genes. Data from qRT-PCR and qPCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in PCR assays was calculated relative to the levels of seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells modulated brown adipogenesis. ( A ) Representative microscopic images of adipocytes of C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ), showing the size of their lipid droplets (400× magnification images, scale bar 30 μm) after oil red O staining, all of them compared with the size of lipid droplets of empty-vector-transfected differentiated cells (VD). qRT-PCR analysis of the indicated brown marker expression levels in the differentiated stably Notch1 -transfected cells (N1SD) ( C ), the differentiated stably Notch2- transfected cells (N2SD) ( D ), the differentiated stably Notch3- transfected cells (N3SD) ( E ), the differentiated stably Notch4- transfected cells (N4SD) ( F ), the differentiated stably Dlk1 -transfected cells (DLK1SD) ( H ) and the differentiated stably Dlk2 -transfected cells (DLK2SD) ( I ). ( G ) qPCR analysis of mtCytb (mitochondrial cytochrome b) DNA amplification (related to genomic ApoB DNA amplification; see Materials and Methods section) in seven-day differentiated C3H10T1/2 cells overexpressing Notch ( G ) and Dlk ( J ) genes. Data from qRT-PCR and qPCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in PCR assays was calculated relative to the levels of seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Techniques Used: Over Expression, Transfection, Staining, Plasmid Preparation, Quantitative RT-PCR, Marker, Expressing, Stable Transfection, Amplification, Activation Assay, Inhibition

    Oxygen consumption rate (OCR) in C3H10T1/2 adipocytes overexpressing Dlk or Notch genes. ( A ) Relative oxygen consumption rate (OCR) in non-differentiated (C3HND) and differentiated (C3HD) C3H10T1/2 cells. Relative oxygen consumption rate (OCR) in differentiated C3H10T1/2 cells overexpressing Dlk ( B ), Notch1 ( C ), Notch2 ( D ), Notch3 ( E ), or Notch4 ( F ) genes. The fold activation or inhibition levels were calculated relative to time 0 of non-differentiated cells, and, additionally, to OCR of non-differentiated cells (ND) ( A ) or seven-day differentiated empty-vector-transfected cells (VD) ( B – F ), which was set arbitrarily at 1. Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated at 144 min (* p ≤ 0.05, ** p ≤ 0.01). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: Oxygen consumption rate (OCR) in C3H10T1/2 adipocytes overexpressing Dlk or Notch genes. ( A ) Relative oxygen consumption rate (OCR) in non-differentiated (C3HND) and differentiated (C3HD) C3H10T1/2 cells. Relative oxygen consumption rate (OCR) in differentiated C3H10T1/2 cells overexpressing Dlk ( B ), Notch1 ( C ), Notch2 ( D ), Notch3 ( E ), or Notch4 ( F ) genes. The fold activation or inhibition levels were calculated relative to time 0 of non-differentiated cells, and, additionally, to OCR of non-differentiated cells (ND) ( A ) or seven-day differentiated empty-vector-transfected cells (VD) ( B – F ), which was set arbitrarily at 1. Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated at 144 min (* p ≤ 0.05, ** p ≤ 0.01). Non-statistical significance is indicated by ns.

    Techniques Used: Activation Assay, Inhibition, Plasmid Preparation, Transfection

    mouse notch1 gene cdna  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
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  • 93

    Structured Review

    ATCC mouse notch1 gene cdna
    Primary and secondary antibodies used in Western blot analysis.
    Mouse Notch1 Gene Cdna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse notch1 gene cdna/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse notch1 gene cdna - by Bioz Stars, 2024-03
    93/100 stars

    Images

    1) Product Images from "NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells"

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    Journal: Cells

    doi: 10.3390/cells9092032

    Primary and secondary antibodies used in Western blot analysis.
    Figure Legend Snippet: Primary and secondary antibodies used in Western blot analysis.

    Techniques Used: Western Blot

    NOTCH signaling in mesenchymal C3H10T1/2 cells stably overexpressing NOTCH receptors or the HES1 protein. ( A ) NOTCH receptors’ transcriptional activity, as measured by luciferase assays, in each of the four stably Notch -transfected pools. The relative luciferase activities were always normalized to renilla levels. ( B ) qRT-PCR analysis of the relative Hes1 and Hey1 mRNA transcription levels in each of the stably Notch -transfected pools. ( C ) qRT-PCR analysis of relative mRNA transcription levels of Notch genes in the stable Notch1 transfectant (N1S), the stable Notch2 transfectant (N2S), the stable Notch3 transfectant (N3S), and the stable Notch4 transfectant (N4S). ( D ) qRT-PCR analysis of Dlk1 (DELTA-like 1 homolog) and Dlk2 (DELTA-like 2 homolog) mRNA transcription levels in the stable Notch transfectants. ( E ) qRT-PCR analysis of the relative Hes1 and Dlk mRNA transcription levels in the stable Hes1 gene transfectant (H1S). All these assays were performed using non-differentiated cells. Data in qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition in all assays was measured relative to levels in non-differentiated empty-vector-transfected cells, set arbitrarily at 1 (horizontal black line or V). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance of Student’s t -test results is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: NOTCH signaling in mesenchymal C3H10T1/2 cells stably overexpressing NOTCH receptors or the HES1 protein. ( A ) NOTCH receptors’ transcriptional activity, as measured by luciferase assays, in each of the four stably Notch -transfected pools. The relative luciferase activities were always normalized to renilla levels. ( B ) qRT-PCR analysis of the relative Hes1 and Hey1 mRNA transcription levels in each of the stably Notch -transfected pools. ( C ) qRT-PCR analysis of relative mRNA transcription levels of Notch genes in the stable Notch1 transfectant (N1S), the stable Notch2 transfectant (N2S), the stable Notch3 transfectant (N3S), and the stable Notch4 transfectant (N4S). ( D ) qRT-PCR analysis of Dlk1 (DELTA-like 1 homolog) and Dlk2 (DELTA-like 2 homolog) mRNA transcription levels in the stable Notch transfectants. ( E ) qRT-PCR analysis of the relative Hes1 and Dlk mRNA transcription levels in the stable Hes1 gene transfectant (H1S). All these assays were performed using non-differentiated cells. Data in qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition in all assays was measured relative to levels in non-differentiated empty-vector-transfected cells, set arbitrarily at 1 (horizontal black line or V). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance of Student’s t -test results is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Techniques Used: Stable Transfection, Activity Assay, Luciferase, Transfection, Quantitative RT-PCR, Activation Assay, Inhibition, Plasmid Preparation

    Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells enhanced adipogenesis levels. Representative microscopic images of adipocytes from C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ) showing their adipogenic levels (50× magnification images, scale bar 200 μm) after oil red O staining, all compared with the adipogenic levels of empty-vector transfected cells (VD). D: differentiated cells. qRT-PCR analysis of indicated adipogenic marker mRNA transcription levels in the differentiated stable Notch1 -overexpressing cells (N1SD) ( C ), the differentiated stable Notch2- overexpressing cells (N2SD) ( D ), the differentiated stable Notch3 -overexpressing cells (N3SD) ( E ), the differentiated stable Notch4- overexpressing cells (N4SD) ( F ), the differentiated stable Dlk1 -overexpressing cells (DLK1SD) ( G ), and the differentiated stable Dlk2 -overexpressing cells (DLK2SD) ( H ). Data from all qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in qRT-PCR assays were calculated relative to the levels shown by seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells enhanced adipogenesis levels. Representative microscopic images of adipocytes from C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ) showing their adipogenic levels (50× magnification images, scale bar 200 μm) after oil red O staining, all compared with the adipogenic levels of empty-vector transfected cells (VD). D: differentiated cells. qRT-PCR analysis of indicated adipogenic marker mRNA transcription levels in the differentiated stable Notch1 -overexpressing cells (N1SD) ( C ), the differentiated stable Notch2- overexpressing cells (N2SD) ( D ), the differentiated stable Notch3 -overexpressing cells (N3SD) ( E ), the differentiated stable Notch4- overexpressing cells (N4SD) ( F ), the differentiated stable Dlk1 -overexpressing cells (DLK1SD) ( G ), and the differentiated stable Dlk2 -overexpressing cells (DLK2SD) ( H ). Data from all qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in qRT-PCR assays were calculated relative to the levels shown by seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Techniques Used: Over Expression, Transfection, Staining, Plasmid Preparation, Quantitative RT-PCR, Marker, Activation Assay, Inhibition

    Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells modulated brown adipogenesis. ( A ) Representative microscopic images of adipocytes of C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ), showing the size of their lipid droplets (400× magnification images, scale bar 30 μm) after oil red O staining, all of them compared with the size of lipid droplets of empty-vector-transfected differentiated cells (VD). qRT-PCR analysis of the indicated brown marker expression levels in the differentiated stably Notch1 -transfected cells (N1SD) ( C ), the differentiated stably Notch2- transfected cells (N2SD) ( D ), the differentiated stably Notch3- transfected cells (N3SD) ( E ), the differentiated stably Notch4- transfected cells (N4SD) ( F ), the differentiated stably Dlk1 -transfected cells (DLK1SD) ( H ) and the differentiated stably Dlk2 -transfected cells (DLK2SD) ( I ). ( G ) qPCR analysis of mtCytb (mitochondrial cytochrome b) DNA amplification (related to genomic ApoB DNA amplification; see Materials and Methods section) in seven-day differentiated C3H10T1/2 cells overexpressing Notch ( G ) and Dlk ( J ) genes. Data from qRT-PCR and qPCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in PCR assays was calculated relative to the levels of seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells modulated brown adipogenesis. ( A ) Representative microscopic images of adipocytes of C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ), showing the size of their lipid droplets (400× magnification images, scale bar 30 μm) after oil red O staining, all of them compared with the size of lipid droplets of empty-vector-transfected differentiated cells (VD). qRT-PCR analysis of the indicated brown marker expression levels in the differentiated stably Notch1 -transfected cells (N1SD) ( C ), the differentiated stably Notch2- transfected cells (N2SD) ( D ), the differentiated stably Notch3- transfected cells (N3SD) ( E ), the differentiated stably Notch4- transfected cells (N4SD) ( F ), the differentiated stably Dlk1 -transfected cells (DLK1SD) ( H ) and the differentiated stably Dlk2 -transfected cells (DLK2SD) ( I ). ( G ) qPCR analysis of mtCytb (mitochondrial cytochrome b) DNA amplification (related to genomic ApoB DNA amplification; see Materials and Methods section) in seven-day differentiated C3H10T1/2 cells overexpressing Notch ( G ) and Dlk ( J ) genes. Data from qRT-PCR and qPCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in PCR assays was calculated relative to the levels of seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Techniques Used: Over Expression, Transfection, Staining, Plasmid Preparation, Quantitative RT-PCR, Marker, Expressing, Stable Transfection, Amplification, Activation Assay, Inhibition

    Oxygen consumption rate (OCR) in C3H10T1/2 adipocytes overexpressing Dlk or Notch genes. ( A ) Relative oxygen consumption rate (OCR) in non-differentiated (C3HND) and differentiated (C3HD) C3H10T1/2 cells. Relative oxygen consumption rate (OCR) in differentiated C3H10T1/2 cells overexpressing Dlk ( B ), Notch1 ( C ), Notch2 ( D ), Notch3 ( E ), or Notch4 ( F ) genes. The fold activation or inhibition levels were calculated relative to time 0 of non-differentiated cells, and, additionally, to OCR of non-differentiated cells (ND) ( A ) or seven-day differentiated empty-vector-transfected cells (VD) ( B – F ), which was set arbitrarily at 1. Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated at 144 min (* p ≤ 0.05, ** p ≤ 0.01). Non-statistical significance is indicated by ns.
    Figure Legend Snippet: Oxygen consumption rate (OCR) in C3H10T1/2 adipocytes overexpressing Dlk or Notch genes. ( A ) Relative oxygen consumption rate (OCR) in non-differentiated (C3HND) and differentiated (C3HD) C3H10T1/2 cells. Relative oxygen consumption rate (OCR) in differentiated C3H10T1/2 cells overexpressing Dlk ( B ), Notch1 ( C ), Notch2 ( D ), Notch3 ( E ), or Notch4 ( F ) genes. The fold activation or inhibition levels were calculated relative to time 0 of non-differentiated cells, and, additionally, to OCR of non-differentiated cells (ND) ( A ) or seven-day differentiated empty-vector-transfected cells (VD) ( B – F ), which was set arbitrarily at 1. Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated at 144 min (* p ≤ 0.05, ** p ≤ 0.01). Non-statistical significance is indicated by ns.

    Techniques Used: Activation Assay, Inhibition, Plasmid Preparation, Transfection

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    ATCC mouse notch1 gene cdna
    Primary and secondary antibodies used in Western blot analysis.
    Mouse Notch1 Gene Cdna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse notch1 gene cdna/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse notch1 gene cdna - by Bioz Stars, 2024-03
    93/100 stars
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    Primary and secondary antibodies used in Western blot analysis.

    Journal: Cells

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    doi: 10.3390/cells9092032

    Figure Lengend Snippet: Primary and secondary antibodies used in Western blot analysis.

    Article Snippet: Plasmid pC-N1 (N1S) contains the complete mouse Notch1 gene cDNA (ATCC clone: MBA-105) in sense orientation [ ].

    Techniques: Western Blot

    NOTCH signaling in mesenchymal C3H10T1/2 cells stably overexpressing NOTCH receptors or the HES1 protein. ( A ) NOTCH receptors’ transcriptional activity, as measured by luciferase assays, in each of the four stably Notch -transfected pools. The relative luciferase activities were always normalized to renilla levels. ( B ) qRT-PCR analysis of the relative Hes1 and Hey1 mRNA transcription levels in each of the stably Notch -transfected pools. ( C ) qRT-PCR analysis of relative mRNA transcription levels of Notch genes in the stable Notch1 transfectant (N1S), the stable Notch2 transfectant (N2S), the stable Notch3 transfectant (N3S), and the stable Notch4 transfectant (N4S). ( D ) qRT-PCR analysis of Dlk1 (DELTA-like 1 homolog) and Dlk2 (DELTA-like 2 homolog) mRNA transcription levels in the stable Notch transfectants. ( E ) qRT-PCR analysis of the relative Hes1 and Dlk mRNA transcription levels in the stable Hes1 gene transfectant (H1S). All these assays were performed using non-differentiated cells. Data in qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition in all assays was measured relative to levels in non-differentiated empty-vector-transfected cells, set arbitrarily at 1 (horizontal black line or V). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance of Student’s t -test results is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Journal: Cells

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    doi: 10.3390/cells9092032

    Figure Lengend Snippet: NOTCH signaling in mesenchymal C3H10T1/2 cells stably overexpressing NOTCH receptors or the HES1 protein. ( A ) NOTCH receptors’ transcriptional activity, as measured by luciferase assays, in each of the four stably Notch -transfected pools. The relative luciferase activities were always normalized to renilla levels. ( B ) qRT-PCR analysis of the relative Hes1 and Hey1 mRNA transcription levels in each of the stably Notch -transfected pools. ( C ) qRT-PCR analysis of relative mRNA transcription levels of Notch genes in the stable Notch1 transfectant (N1S), the stable Notch2 transfectant (N2S), the stable Notch3 transfectant (N3S), and the stable Notch4 transfectant (N4S). ( D ) qRT-PCR analysis of Dlk1 (DELTA-like 1 homolog) and Dlk2 (DELTA-like 2 homolog) mRNA transcription levels in the stable Notch transfectants. ( E ) qRT-PCR analysis of the relative Hes1 and Dlk mRNA transcription levels in the stable Hes1 gene transfectant (H1S). All these assays were performed using non-differentiated cells. Data in qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition in all assays was measured relative to levels in non-differentiated empty-vector-transfected cells, set arbitrarily at 1 (horizontal black line or V). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance of Student’s t -test results is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Article Snippet: Plasmid pC-N1 (N1S) contains the complete mouse Notch1 gene cDNA (ATCC clone: MBA-105) in sense orientation [ ].

    Techniques: Stable Transfection, Activity Assay, Luciferase, Transfection, Quantitative RT-PCR, Activation Assay, Inhibition, Plasmid Preparation

    Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells enhanced adipogenesis levels. Representative microscopic images of adipocytes from C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ) showing their adipogenic levels (50× magnification images, scale bar 200 μm) after oil red O staining, all compared with the adipogenic levels of empty-vector transfected cells (VD). D: differentiated cells. qRT-PCR analysis of indicated adipogenic marker mRNA transcription levels in the differentiated stable Notch1 -overexpressing cells (N1SD) ( C ), the differentiated stable Notch2- overexpressing cells (N2SD) ( D ), the differentiated stable Notch3 -overexpressing cells (N3SD) ( E ), the differentiated stable Notch4- overexpressing cells (N4SD) ( F ), the differentiated stable Dlk1 -overexpressing cells (DLK1SD) ( G ), and the differentiated stable Dlk2 -overexpressing cells (DLK2SD) ( H ). Data from all qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in qRT-PCR assays were calculated relative to the levels shown by seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Journal: Cells

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    doi: 10.3390/cells9092032

    Figure Lengend Snippet: Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells enhanced adipogenesis levels. Representative microscopic images of adipocytes from C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ) showing their adipogenic levels (50× magnification images, scale bar 200 μm) after oil red O staining, all compared with the adipogenic levels of empty-vector transfected cells (VD). D: differentiated cells. qRT-PCR analysis of indicated adipogenic marker mRNA transcription levels in the differentiated stable Notch1 -overexpressing cells (N1SD) ( C ), the differentiated stable Notch2- overexpressing cells (N2SD) ( D ), the differentiated stable Notch3 -overexpressing cells (N3SD) ( E ), the differentiated stable Notch4- overexpressing cells (N4SD) ( F ), the differentiated stable Dlk1 -overexpressing cells (DLK1SD) ( G ), and the differentiated stable Dlk2 -overexpressing cells (DLK2SD) ( H ). Data from all qRT-PCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in qRT-PCR assays were calculated relative to the levels shown by seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Article Snippet: Plasmid pC-N1 (N1S) contains the complete mouse Notch1 gene cDNA (ATCC clone: MBA-105) in sense orientation [ ].

    Techniques: Over Expression, Transfection, Staining, Plasmid Preparation, Quantitative RT-PCR, Marker, Activation Assay, Inhibition

    Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells modulated brown adipogenesis. ( A ) Representative microscopic images of adipocytes of C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ), showing the size of their lipid droplets (400× magnification images, scale bar 30 μm) after oil red O staining, all of them compared with the size of lipid droplets of empty-vector-transfected differentiated cells (VD). qRT-PCR analysis of the indicated brown marker expression levels in the differentiated stably Notch1 -transfected cells (N1SD) ( C ), the differentiated stably Notch2- transfected cells (N2SD) ( D ), the differentiated stably Notch3- transfected cells (N3SD) ( E ), the differentiated stably Notch4- transfected cells (N4SD) ( F ), the differentiated stably Dlk1 -transfected cells (DLK1SD) ( H ) and the differentiated stably Dlk2 -transfected cells (DLK2SD) ( I ). ( G ) qPCR analysis of mtCytb (mitochondrial cytochrome b) DNA amplification (related to genomic ApoB DNA amplification; see Materials and Methods section) in seven-day differentiated C3H10T1/2 cells overexpressing Notch ( G ) and Dlk ( J ) genes. Data from qRT-PCR and qPCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in PCR assays was calculated relative to the levels of seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Journal: Cells

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    doi: 10.3390/cells9092032

    Figure Lengend Snippet: Stable overexpression of Notch and Dlk genes in multipotent C3H10T1/2 cells modulated brown adipogenesis. ( A ) Representative microscopic images of adipocytes of C3H10T1/2 cells transfected with Notch ( A ) or Dlk genes ( B ), showing the size of their lipid droplets (400× magnification images, scale bar 30 μm) after oil red O staining, all of them compared with the size of lipid droplets of empty-vector-transfected differentiated cells (VD). qRT-PCR analysis of the indicated brown marker expression levels in the differentiated stably Notch1 -transfected cells (N1SD) ( C ), the differentiated stably Notch2- transfected cells (N2SD) ( D ), the differentiated stably Notch3- transfected cells (N3SD) ( E ), the differentiated stably Notch4- transfected cells (N4SD) ( F ), the differentiated stably Dlk1 -transfected cells (DLK1SD) ( H ) and the differentiated stably Dlk2 -transfected cells (DLK2SD) ( I ). ( G ) qPCR analysis of mtCytb (mitochondrial cytochrome b) DNA amplification (related to genomic ApoB DNA amplification; see Materials and Methods section) in seven-day differentiated C3H10T1/2 cells overexpressing Notch ( G ) and Dlk ( J ) genes. Data from qRT-PCR and qPCR assays were normalized to P0 mRNA transcription levels. The fold activation or inhibition levels in PCR assays was calculated relative to the levels of seven-day differentiated empty-vector-transfected cells, which were set arbitrarily at 1 (horizontal black line). Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Non-statistical significance is indicated by ns.

    Article Snippet: Plasmid pC-N1 (N1S) contains the complete mouse Notch1 gene cDNA (ATCC clone: MBA-105) in sense orientation [ ].

    Techniques: Over Expression, Transfection, Staining, Plasmid Preparation, Quantitative RT-PCR, Marker, Expressing, Stable Transfection, Amplification, Activation Assay, Inhibition

    Oxygen consumption rate (OCR) in C3H10T1/2 adipocytes overexpressing Dlk or Notch genes. ( A ) Relative oxygen consumption rate (OCR) in non-differentiated (C3HND) and differentiated (C3HD) C3H10T1/2 cells. Relative oxygen consumption rate (OCR) in differentiated C3H10T1/2 cells overexpressing Dlk ( B ), Notch1 ( C ), Notch2 ( D ), Notch3 ( E ), or Notch4 ( F ) genes. The fold activation or inhibition levels were calculated relative to time 0 of non-differentiated cells, and, additionally, to OCR of non-differentiated cells (ND) ( A ) or seven-day differentiated empty-vector-transfected cells (VD) ( B – F ), which was set arbitrarily at 1. Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated at 144 min (* p ≤ 0.05, ** p ≤ 0.01). Non-statistical significance is indicated by ns.

    Journal: Cells

    Article Title: NOTCH Receptors and DLK Proteins Enhance Brown Adipogenesis in Mesenchymal C3H10T1/2 Cells

    doi: 10.3390/cells9092032

    Figure Lengend Snippet: Oxygen consumption rate (OCR) in C3H10T1/2 adipocytes overexpressing Dlk or Notch genes. ( A ) Relative oxygen consumption rate (OCR) in non-differentiated (C3HND) and differentiated (C3HD) C3H10T1/2 cells. Relative oxygen consumption rate (OCR) in differentiated C3H10T1/2 cells overexpressing Dlk ( B ), Notch1 ( C ), Notch2 ( D ), Notch3 ( E ), or Notch4 ( F ) genes. The fold activation or inhibition levels were calculated relative to time 0 of non-differentiated cells, and, additionally, to OCR of non-differentiated cells (ND) ( A ) or seven-day differentiated empty-vector-transfected cells (VD) ( B – F ), which was set arbitrarily at 1. Data are shown as the mean ± SD of at least three biological assays performed in triplicate. The statistical significance calculated by Student’s t -test is indicated at 144 min (* p ≤ 0.05, ** p ≤ 0.01). Non-statistical significance is indicated by ns.

    Article Snippet: Plasmid pC-N1 (N1S) contains the complete mouse Notch1 gene cDNA (ATCC clone: MBA-105) in sense orientation [ ].

    Techniques: Activation Assay, Inhibition, Plasmid Preparation, Transfection