mouse myoblast cell line c2c12  (ATCC)

Journal: Biomedical Reports

Article Title: Resveratrol ameliorates high‑fat diet‑induced insulin resistance via the DDIT4/mTOR pathway in skeletal muscle

doi: 10.3892/br.2025.1977

Figure Lengend Snippet: Expression of DDIT4, mTOR, GLUT4, PI3K, and AKT in MHY1485-treated IR C2C12 cells. (A) Protein expression levels of DDIT4, mTOR, GLUT4, PI3K, AKT after the activation of mTOR. (B) Semi-quantification of p-protein/t-protein ratios for mTOR, PI3K, AKT and protein expression levels of DDIT4, GLUT4 after MHY1485 treatment. (C) Glucose levels in culture medium after MHY1485 treatment. (D) Cell TG levels after MHY1485 treatment. Data are presented as the mean ± SD (n=3). * P<0.05 vs. the CON group; # P<0.05 vs. the PA group; & P<0.05 vs. the PA + RSV group. DDIT4, DNA-damage-inducible transcript 4; mTOR, mammalian target of rapamycin; GLUT4, glucose transporter 4; PI3K, phosphatidylinositol 3-kinase; AKT, protein kinase B; IR, insulin resistance; p-, phosphorylated; t-, total; TG, triglyceride; CON, control; PA, palmitic acid; RSV, resveratrol.

Article Snippet: The C2C12 mouse myoblast cell line (cat. no. CL-0044; Procell Life Science & Technology Co., Ltd.) was cultured in culture medium (cat. no. CM-0044 Procell Life Science & Technology Co., Ltd.) until the cells reached 70-80% confluence.

Techniques: Expressing, Activation Assay, Control

Journal: Biomedical Reports

Article Title: Resveratrol ameliorates high‑fat diet‑induced insulin resistance via the DDIT4/mTOR pathway in skeletal muscle

doi: 10.3892/br.2025.1977

Figure Lengend Snippet: Expression levels of DDIT4, mTOR, GLUT4, PI3K, and AKT in DDIT4-siRNA-treated IR C2C12 cells. (A) DDIT4-siRNA-1 had the most significant silencing effect. (B) Protein expression levels of DDIT4, mTOR, GLUT4, PI3K, and AKT after DDIT4-siRNA treatment. (C) Semi-quantification of p-protein/t-protein ratios for mTOR, PI3K, and AKT and protein expression levels of DDIT4 and GLUT4 after DDIT4-siRNA treatment. (D) Glucose levels in culture medium after DDIT4-siRNA treatment. (E) TG levels of C2C12 cells after DDIT4-siRNA treatment. Data are presented as the mean ± SD (n=3). * P<0.05 vs. the CON group; # P<0.05 vs. the PA group; & P<0.05 vs. the PA + RSV group. DDIT4, DNA-damage-inducible transcript 4; mTOR, mammalian target of rapamycin; GLUT4, glucose transporter 4; PI3K, phosphatidylinositol 3-kinase; AKT, protein kinase B; siRNA, small interfering RNA; IR, insulin resistance; p-, phosphorylated; t-, total; TG, triglyceride; CON, control; PA, palmitic acid; RSV, resveratrol.

Article Snippet: The C2C12 mouse myoblast cell line (cat. no. CL-0044; Procell Life Science & Technology Co., Ltd.) was cultured in culture medium (cat. no. CM-0044 Procell Life Science & Technology Co., Ltd.) until the cells reached 70-80% confluence.

Techniques: Expressing, Small Interfering RNA, Control

Journal: Calcified Tissue International

Article Title: Roles of Insulin-Like Growth Factor-1 in Muscle Wasting and Osteopenia in Mice with Hyponatremia

doi: 10.1007/s00223-025-01369-7

Figure Lengend Snippet: Effects of low sodium concentrations on IGF-1 expression in C2C12 cells. A , B Total RNA was extracted from C2C12 myoblasts ( A ) and myotubes ( B ) 24 or 72 h after culture in medium containing indicated concentrations of sodium, and real-time PCR analysis of IGF-1 and 18S rRNA was performed. Data represent the mean ± SEM (n = 5 sample in each group). Cont; Control. ** P < 0.01 and * P < 0.05

Article Snippet: Mouse myoblastic C2C12 cells (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Wako Pure Chemicals) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control