mouse leukocytes murine peritoneal macrophage cell line raw 264 7  (ATCC)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

doi: 10.1155/2012/743075

Figure Lengend Snippet: Inhibitory effects on NO and TARC by Angelicae Dahuricae Radix (AR). The concentrations of NO and TARC in the supernatant were determined using NO and TARC ELISA assays. (a) RAW 264.7 cells were treated with LPS (1 μ g/mL) for 18 hr. AR inhibited NO production in a concentration-dependent manner (mean ± SEM ( n = 5), # : P < 0.05, ## : P < 0.01 compared with control group, *: P < 0.05, **: P < 0.01 compared with the LPS-treated group). (b) HaCaT cells were treated with TNF- α plus IFN- γ (TI) (10 ng/mL each) for 24 h. TARC levels were significantly suppressed by AR (mean ± SEM, n = 5), # : P < 0.05, ## : P < 0.01 compared with control group, *: P < 0.05, **: P < 0.01 compared with TI treatment. (c) The MDC and TARC mRNA expression levels were determined by RT-PCR analysis, and AR suppressed the mRNA expression of MDC and TARC. The cells were treated with silymarin (10, 20, or 50 μ g/mL), forskolin (5, 10, or 30 μ g/mL), or AR (60, 150, or 300 μ g/mL).

Article Snippet: The murine macrophage cell line RAW 264.7 was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: PPAR Research

Article Title: Investigation of PPAR β / δ within Human Dental Pulp Cells: A Preliminary In Vitro Study

doi: 10.1155/2021/8854921

Figure Lengend Snippet: GW0742-activated PPAR β / δ represses cytokine gene expression in LPS-stimulated hDPCs: (a) treatment with GW0742 (0.01, 0.1, and 1.0 μ M) did not alter hDPC viability. (b) Real-time qPCR analyses of IL6 , IL1β , and TNFα in cultured hDPCs pretreated with GW0742 (for 24 h) and added LPS (2 μ g/mL) for 4 h before harvesting. GW0742 significantly reduced LPS-induced IL6 and IL1β at 0.1 μ M and LPS-induced IL6 , IL1β , and TNFα at 1.0 μ M. (c) Pretreatment with 0.01 μ M GW0742 (for 24 h) significantly reduced Il6 and Tnfα mRNA levels in LPS-stimulated RAW264.7 cells (for 24 h) ( p < 0.05; # vs. control; ∗ vs. LPS by Kruskal–Wallis and post hoc Dunn's test; mean ± S.E.M. n = per group). Control = DMSO 0.1%.

Article Snippet: The RAW264.7 murine macrophage cell line was purchased from the American Type Culture Collection (ATCC® TIB71™, Manassas, VA) and kindly provided by Dr. Paul Webb from the Methodist Research Institute, Houston, TX.

Techniques: Expressing, Cell Culture

Journal: PPAR Research

Article Title: Investigation of PPAR β / δ within Human Dental Pulp Cells: A Preliminary In Vitro Study

doi: 10.1155/2021/8854921

Figure Lengend Snippet: GW0742-activated PPAR β / δ represses MMP gene expression and gelatinolytic activity: (a) pretreatment with GW0742 repressed MMP1 and MMP2 gene expression and gelatinolytic activity of (b) MMP2 and (c) MMP9 in LSP-stimulated hDPCs ( p < 0.05; # vs. control; ∗ vs. LPS by Kruskal–Wallis and post hoc Dunn's test; mean ± S.E.M. n = per group). Control = DMSO 0.1%. +C = positive control, supernatant from macrophage RAW264.7 cells.

Article Snippet: The RAW264.7 murine macrophage cell line was purchased from the American Type Culture Collection (ATCC® TIB71™, Manassas, VA) and kindly provided by Dr. Paul Webb from the Methodist Research Institute, Houston, TX.

Techniques: Expressing, Activity Assay, Positive Control

Journal: PPAR Research

Article Title: Investigation of PPAR β / δ within Human Dental Pulp Cells: A Preliminary In Vitro Study

doi: 10.1155/2021/8854921

Figure Lengend Snippet: Pretreatment with GW0742 downregulates LPS-stimulated hDPCs' chemotactic ability: LPS-stimulated hDPCs recruited more RAW264.7 macrophage cells when compared with control cells. Pretreatment of LPS-stimulated hDPCs with 1.0 μ M GW0742 significantly reduced the number of migrated macrophage cells ( p < 0.05; # vs. control; ∗ vs. LPS by one-way ANOVA and post hoc Newman−Keuls; mean ± S.E.M. n = per group).

Article Snippet: The RAW264.7 murine macrophage cell line was purchased from the American Type Culture Collection (ATCC® TIB71™, Manassas, VA) and kindly provided by Dr. Paul Webb from the Methodist Research Institute, Houston, TX.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity

doi: 10.2147/IJN.S191689

Figure Lengend Snippet: Cellular uptake of three types of MWCNTs with or without BSA ( A ) and IgG ( B ) coronas by RAW264.7 cells. Notes: Quantitative analysis of cellular uptake of MWCNTs by RAW264.7 cells as shown by the standard turbidimetric method. Cells were cultured in 6-well culture dishes to confluence and exposed to MWCNTs (25 µg/mL) up to 24 hours. Dimethyl sulfoxide (0.2 mL) was added to each well and the lysate was pipetted thoroughly. A 250 µL sample was transferred to 96-well plates, and the OD (640 nm) was measured to quantify the amount of cell-associated MWCNTs. Data are representative of three independent experiments. * P <0.05 compared to the same types of MWCNTs without protein corona BSA: human plasma protein; IgG: human serum albumin. Abbreviations: IgG, immunoglobulin G; MWCNTs, multiwalled carbon nanotubes; MWCNTs-COOH; carboxylated MWCNTs; MWCNTs-PEG, polyethylene glycol MWCNTs.

Article Snippet: The murine macrophage cell line RAW264.7 was obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in high glucose Dulbecco’s minimum essential medium (DMEM) with 10% FBS at 37°C and 5% CO 2 .

Techniques: Cell Culture

Journal: International Journal of Nanomedicine

Article Title: MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity

doi: 10.2147/IJN.S191689

Figure Lengend Snippet: Cytotoxicity in RAW264.7 cells exposed to three types of MWCNTs with or without BSA ( A ) and IgG ( B ) coronas. Notes: Assessment of cytotoxicity of MWCNTs with WST-8 and LDH assays. After exposure to 25, 50, and 100 µg/mL of each of the MWCNT suspensions for 24 hours, RAW264.7 cells were incubated with the WST-8 reagent for 1 hour, and the absorbance was measured at 490 nm. All the WST-8 values were normalized according to the control (no MWCNT exposure), which was regarded as the 100% cell viability reference point. Similar to the treatment, cell supernatants from control and exposure experiments were collected and assayed for LDH activity as described in the Materials and methods section. Data are representative of three separate experiments with at least three wells per treatment. All the WST-8 and LDH values were normalized according to the nontreated control, which was regarded as representing 100% cell viability. * P <0.05 compared to control cells, # P <0.05 compared to the same types of MWCNTs without protein corona. ( C ) Morphology of RAW264.7 cells treated with three types of MWCNTs (25 µg/mL) in FBS free and complete medium for 6 hours. The asterisks indicate MWCNTs agglomerates to adhere to cell walls and shrinkage of the cell membrane. The scale bar is 50 µm. Abbreviations: IgG, immunoglobulin G; LDH, lactate dehydrogenase; MWCNTs, multiwalled carbon nanotubes; MWCNTs-COOH; carboxylated MWCNTs; MWCNTs-PEG, polyethylene glycol MWCNTs.

Article Snippet: The murine macrophage cell line RAW264.7 was obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in high glucose Dulbecco’s minimum essential medium (DMEM) with 10% FBS at 37°C and 5% CO 2 .

Techniques: Incubation, Activity Assay

Journal: International Journal of Nanomedicine

Article Title: MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity

doi: 10.2147/IJN.S191689

Figure Lengend Snippet: Pro-inflammatory cytokine production in RAW264.7 cells in response to three types of MWCNTs with or without BSA and IgG coronas. Notes: IL-1β ( A ) and TNF-α ( B ) production in RAW264.7 cells. RAW264.7 cells were exposed to 0, 25, and 100 µg/mL MWCNTs with or without BSA and IgG coronas for 24 hours to determine IL-1β and TNF-α release into the supernatants was assessed by ELISA. Control cells were not subjected to MWCNTs exposure. * P <0.05 compared to control cells, # P <0.05 compared to the same types of MWCNTs without protein corona. Abbreviations: IgG, immunoglobulin G; MWCNTs, multiwalled carbon nanotubes; MWCNTs-COOH, carboxylated MWCNTs; MWCNTs-PEG, polyethylene glycol MWCNTs; TNF-α, tumor necrosis factor α.

Article Snippet: The murine macrophage cell line RAW264.7 was obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in high glucose Dulbecco’s minimum essential medium (DMEM) with 10% FBS at 37°C and 5% CO 2 .

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Nanomedicine

Article Title: MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity

doi: 10.2147/IJN.S191689

Figure Lengend Snippet: Inhibition of endocytosis by cytochalasin D that attenuates the toxicity of MWCNTs. Notes: Raw264.7 cells preincubated with endocytosis inhibitor, Cytochalasin D (CytoD) for 2 hours, and investigated the cellular uptake, cytotoxicity and pro-inflammatory cytokine secretion after 4 µM CytoD inhibitor pretreatment with bare or BSA coronas MWCNTs. ( A ) Cellular uptake of Raw264.7 cells was analyzed after the treatment of MWCNTs for 6 hours. Cytotoxicity was measured by WST-8 ( B ) and LHD assays ( C ). Levels of IL-1β ( D ) of RAW264.7 cells at 24 hours following the treatment. All assays were performed as described in Materials and methods section. Control cells were not subjected to MWCNTs exposure. * P <0.05 compared to control cells, # P <0.05 compared to the same types of MWCNTs with CytoD inhibitor pretreatment. Abbreviations: IgG, immunoglobulin G; LDH, lactate dehydrogenase; MWCNTs, multiwalled carbon nanotubes; MWCNTs-COOH, carboxylated MWCNTs; MWCNTs-PEG, polyethylene glycol MWCNTs.

Article Snippet: The murine macrophage cell line RAW264.7 was obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in high glucose Dulbecco’s minimum essential medium (DMEM) with 10% FBS at 37°C and 5% CO 2 .

Techniques: Inhibition

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo Characterization of Host–Pathogen Interactions of the L3881 Candida albicans Clinical Isolate

doi: 10.3389/fmicb.2022.901442

Figure Lengend Snippet: In vitro characterization of Candida albicans SC5314 and L3881 lineages. (A) Phagocytosis was assessed by cytospin preparations of murine macrophage RAW 264.7 cell line co-cultured with SC5314 and L3881. (B) Killing assay was evaluated by cell lysis with water, the diluted samples were plated in the fungal medium, and colony-forming units (CFUs) were determined 16 h post-incubation (PI). Each dot represents an experimental replica. Data are presented as mean ± standard deviation (SD). Asterisk (*) represents significant differences with p < 0.05.

Article Snippet: The murine macrophage RAW 264.7 cell line was obtained from the American Type Culture Collection and cultivated as described in .

Techniques: In Vitro, Cell Culture, Lysis, Incubation, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo Characterization of Host–Pathogen Interactions of the L3881 Candida albicans Clinical Isolate

doi: 10.3389/fmicb.2022.901442

Figure Lengend Snippet: In vitro cytokine and chemokine profile of L3881 and SC5314. Murine macrophage RAW 264.7 cell line was infected with L3881 and SC5314 yeast lineages. Supernatants were collected at 2 and 4 h post-incubation and used for ELISA for quantifications of (A) TNF-α, (B) CCL2, (C) IFN-γ, (D) CXCL1, and (E) IL-4 levels. Each dot represents an experimental replica. Data are presented as mean ± SD. Hashtag (#) and asterisk (*) represent significant differences with p < 0.05.

Article Snippet: The murine macrophage RAW 264.7 cell line was obtained from the American Type Culture Collection and cultivated as described in .

Techniques: In Vitro, Infection, Incubation, Enzyme-linked Immunosorbent Assay