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mouse monoclonal primary antibody against cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal primary antibody against cyclin d1
    Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, <t>cyclin</t> <t>D1,</t> and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.
    Mouse Monoclonal Primary Antibody Against Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal primary antibody against cyclin d1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal primary antibody against cyclin d1 - by Bioz Stars, 2024-10
    96/100 stars

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    1) Product Images from "A novel synthetic Asiatic acid derivative induces apoptosis and inhibits proliferation and mobility of gastric cancer cells by suppressing STAT3 signaling pathway"

    Article Title: A novel synthetic Asiatic acid derivative induces apoptosis and inhibits proliferation and mobility of gastric cancer cells by suppressing STAT3 signaling pathway

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S121619

    Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, cyclin D1, and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.
    Figure Legend Snippet: Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, cyclin D1, and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.

    Techniques Used: Transfection, shRNA, Expressing, Plasmid Preparation, Western Blot



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    mouse monoclonal primary antibody against cyclin d1  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc mouse monoclonal primary antibody against cyclin d1
    Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, <t>cyclin</t> <t>D1,</t> and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.
    Mouse Monoclonal Primary Antibody Against Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal primary antibody against cyclin d1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal primary antibody against cyclin d1 - by Bioz Stars, 2024-10
    96/100 stars
    		  Buy from Supplier
    mouse monoclonal primary antibodies against cyclin d1  (Biogenex)
    86
    Biogenex mouse monoclonal primary antibodies against cyclin d1
    Of cases showing of <t> cyclin </t> <t> D1, </t> p27 and p63 immunohistochemical expression based on staining intensity and distribution in mild moderate and severe dysplasia cases
    Mouse Monoclonal Primary Antibodies Against Cyclin D1, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal primary antibodies against cyclin d1/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal primary antibodies against cyclin d1 - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, cyclin D1, and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.

    Journal: OncoTargets and therapy

    Article Title: A novel synthetic Asiatic acid derivative induces apoptosis and inhibits proliferation and mobility of gastric cancer cells by suppressing STAT3 signaling pathway

    doi: 10.2147/OTT.S121619

    Figure Lengend Snippet: Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, cyclin D1, and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.

    Article Snippet: Rabbit polyclonal primary antibodies to phospho-STAT3(Tyr 705 ), JAK2, phospho-JAK2, c-Myc, MMP-2, and MMP-9, and mouse monoclonal primary antibody against cyclin D1 were all obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Transfection, shRNA, Expressing, Plasmid Preparation, Western Blot

    Journal: Journal of Natural Science, Biology, and Medicine

    Article Title: Immunohistochemical evaluation of oral epithelial dysplasia using cyclin-D1, p27 and p63 expression as predictors of malignant transformation

    doi: 10.4103/0976-9668.117011

    Figure Lengend Snippet: Of cases showing of cyclin D1, p27 and p63 immunohistochemical expression based on staining intensity and distribution in mild moderate and severe dysplasia cases

    Article Snippet: The sections were incubated with precisely diluted mouse monoclonal primary antibodies against cyclin D1 (Biogenex, USA), p27 (Biogenex, USA) and p63 (Biogenex, USA) at 37˚C temperature for 60 min respectively for all the cases in a humid chamber.

    Techniques: Immunohistochemical staining, Expressing, Staining

    Weak to moderate immunostaining of areas of mild dysplasia with cyclin D1 in basal, parabasal and minimally in suprabasal cells under ×10 magnification

    Journal: Journal of Natural Science, Biology, and Medicine

    Article Title: Immunohistochemical evaluation of oral epithelial dysplasia using cyclin-D1, p27 and p63 expression as predictors of malignant transformation

    doi: 10.4103/0976-9668.117011

    Figure Lengend Snippet: Weak to moderate immunostaining of areas of mild dysplasia with cyclin D1 in basal, parabasal and minimally in suprabasal cells under ×10 magnification

    Article Snippet: The sections were incubated with precisely diluted mouse monoclonal primary antibodies against cyclin D1 (Biogenex, USA), p27 (Biogenex, USA) and p63 (Biogenex, USA) at 37˚C temperature for 60 min respectively for all the cases in a humid chamber.

    Techniques: Immunostaining

    Strong immunostaining of areas of moderate dysplasia with cyclin D1 in basal cells with basilar hyperplasia, parabasal and suprabasal cells under ×10 magnification

    Journal: Journal of Natural Science, Biology, and Medicine

    Article Title: Immunohistochemical evaluation of oral epithelial dysplasia using cyclin-D1, p27 and p63 expression as predictors of malignant transformation

    doi: 10.4103/0976-9668.117011

    Figure Lengend Snippet: Strong immunostaining of areas of moderate dysplasia with cyclin D1 in basal cells with basilar hyperplasia, parabasal and suprabasal cells under ×10 magnification

    Article Snippet: The sections were incubated with precisely diluted mouse monoclonal primary antibodies against cyclin D1 (Biogenex, USA), p27 (Biogenex, USA) and p63 (Biogenex, USA) at 37˚C temperature for 60 min respectively for all the cases in a humid chamber.

    Techniques: Immunostaining