Journal: The Journal of Biological Chemistry
Article Title: Novel MASP-2 inhibitors developed via directed evolution of human TFPI1 are potent lectin pathway inhibitors
doi: 10.1074/jbc.RA119.008315
Figure Lengend Snippet: LP-inhibitory effects of TFMI-2b and SGMI-2 in complement deposition ELISA with rat serum. Shown are LP-inhibitory effects of TFMI-2b (●) and SGMI-2 (▾) in C3 deposition ELISA on a mannan-coated surface with 70-fold diluted rat serum ( A ), C4 deposition ELISA on a mannan-coated surface with 60-fold diluted rat serum ( B ), and C5b-9 deposition ELISA on a mannan-coated surface with 50-fold diluted rat serum ( C ). Data points represent the average of two experiments, whereas the error bars represent the S.E.
Article Snippet: Monoclonal mouse anti-rat C5b-9 antibody (sc-66190, Santa Cruz Biotechnology, Inc.) was used as primary antibody in a 1,000-fold dilution, and peroxidase-conjugated anti-mouse polyclonal antibody (AP308P, Merck) was used as secondary antibody in a 3,000-fold dilution.
Techniques: Enzyme-linked Immunosorbent Assay