Journal: eLife

Article Title: JAK inhibition decreases the autoimmune burden in Down syndrome

doi: 10.7554/eLife.99323

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-human CD34 (clone 581) , Fluidigm , Cat # 3163014B; RRID: AB_2811091 , Lot 2651705, 1:33.

Techniques: Isolation, Biomarker Assay, Staining, Antibody Labeling, Software, Sequencing

Journal: iScience

Article Title: Multimodal single-cell profiling reveals neuronal vulnerability and pathological cell states in focal cortical dysplasia

doi: 10.1016/j.isci.2024.111337

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Article Snippet: Mouse monoclonal anti-CD34 , Dako , Cat#M7165; RRID: AB_2063006.

Techniques: Recombinant, Expressing, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining