Structured Review

Santa Cruz Biotechnology monoclonal anti ace2 mouse igg
Morphological analysis and development of BEAS-CTL and <t>BEAS-ACE2</t> spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
Monoclonal Anti Ace2 Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal anti ace2 mouse igg/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monoclonal anti ace2 mouse igg - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection"

Article Title: Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection

Journal: Biomaterials and Biosystems

doi: 10.1016/j.bbiosy.2023.100082

Morphological analysis and development of BEAS-CTL and BEAS-ACE2 spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
Figure Legend Snippet: Morphological analysis and development of BEAS-CTL and BEAS-ACE2 spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.

Techniques Used:

MatriWells representative scheme and SARS-CoV-2 kinetics in BEAS-ACE2 MatriWells and HUVEC cells . (A) Pulmonary ALI creation using the MatriWells, BEAS-ACE2 and HUVEC cells. (B) Intracellular viral load in BEAS-ACE2 MatriWells. (C) Number of viral particles per mL(PFU[plaque-forming units]/mL) in HUVEC's supernatant. (D) Intracellular viral load in HUVEC cells. *p ≤ 0.05; ***p ≤ 0.001. n = 12.
Figure Legend Snippet: MatriWells representative scheme and SARS-CoV-2 kinetics in BEAS-ACE2 MatriWells and HUVEC cells . (A) Pulmonary ALI creation using the MatriWells, BEAS-ACE2 and HUVEC cells. (B) Intracellular viral load in BEAS-ACE2 MatriWells. (C) Number of viral particles per mL(PFU[plaque-forming units]/mL) in HUVEC's supernatant. (D) Intracellular viral load in HUVEC cells. *p ≤ 0.05; ***p ≤ 0.001. n = 12.

Techniques Used:

BEAS-CTL and BEAS-ACE2 spheroids viability over time . The Viability over time was quantified using the trypan blue staining. *p ≤ 0.05, n = 3.
Figure Legend Snippet: BEAS-CTL and BEAS-ACE2 spheroids viability over time . The Viability over time was quantified using the trypan blue staining. *p ≤ 0.05, n = 3.

Techniques Used: Staining

Viral load quantification and immunofluorescence assay of BEAS-CTL and BEAS-ACE2 spheroids . (A) Spheroid intracellular viral concentration. (B) Immunofluorescence microscopy images of the spheroids with nucleus (Hoechst), ACE2, and Spike protein staining. Fluorescence quantification of (C) Spike protein and (D) ACE2 expression. (E) Pearson correlation comparing ACE2 and Spike protein expression. Scale bar: 50 µm. *p ≤ 0.05; **p ≤ 0.01. n = 3-5
Figure Legend Snippet: Viral load quantification and immunofluorescence assay of BEAS-CTL and BEAS-ACE2 spheroids . (A) Spheroid intracellular viral concentration. (B) Immunofluorescence microscopy images of the spheroids with nucleus (Hoechst), ACE2, and Spike protein staining. Fluorescence quantification of (C) Spike protein and (D) ACE2 expression. (E) Pearson correlation comparing ACE2 and Spike protein expression. Scale bar: 50 µm. *p ≤ 0.05; **p ≤ 0.01. n = 3-5

Techniques Used: Immunofluorescence, Concentration Assay, Microscopy, Staining, Fluorescence, Expressing

SARS-CoV-2 infection of the 3D models . (A) SARS-CoV-2 infection of spheroids and MatriWells. (B) Comparison of intracellular viral load in BEAS-CTL spheroids and MatriWells. (C) Comparison of intracellular viral load in BEAS-ACE2 spheroids and MatriWells. ****p ≤ 0.0001, *p≤0.05. n = 5-15
Figure Legend Snippet: SARS-CoV-2 infection of the 3D models . (A) SARS-CoV-2 infection of spheroids and MatriWells. (B) Comparison of intracellular viral load in BEAS-CTL spheroids and MatriWells. (C) Comparison of intracellular viral load in BEAS-ACE2 spheroids and MatriWells. ****p ≤ 0.0001, *p≤0.05. n = 5-15

Techniques Used: Infection


Structured Review

Santa Cruz Biotechnology mouse monoclonal anti ace2
Mouse Monoclonal Anti Ace2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti ace2/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal anti ace2 - by Bioz Stars, 2024-06
86/100 stars

Images


Structured Review

Santa Cruz Biotechnology mouse monoclonal anti ace2
Distribution of <t>ACE2</t> receptor and attachment of SARS-CoV-2 and human coronavirus (HCoV-NL63) to tissue sections of human trachea. These images show formalin-fixed paraffin-embedded cross-sections of human tracheal sections stained with ImmPRESS VR anti-mouse/rabbit IgG horseradish peroxidase (HRP) polymer detection kit. Dark brown represents the presence of a protein interacting with a specific antibody and is considered a positive expression. Pale brown background, and the nucleus counterstained with hematoxylin is blue. ( a ) Expression of ACE2 in human trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL); and ( d ) corresponding negative control tissue sections stained with secondary anti-mouse HRP antibody only. ( b , c , e , f ) The tissues sections presented in this panel show virus binding in formalin-fixed paraffin-embedded cross-sections after overnight incubation with 250 μL ( b ) heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 or ( c ) HCoV-NL63. ( e , f ) Virus-incubated sections that were stained with secondary anti-rabbit HRP antibody only. Scale bar—100 μm.
Mouse Monoclonal Anti Ace2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti ace2/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal anti ace2 - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "SARS-CoV-2 Is More Efficient than HCoV-NL63 in Infecting a Small Subpopulation of ACE2+ Human Respiratory Epithelial Cells"

Article Title: SARS-CoV-2 Is More Efficient than HCoV-NL63 in Infecting a Small Subpopulation of ACE2+ Human Respiratory Epithelial Cells

Journal: Viruses

doi: 10.3390/v15030736

Distribution of ACE2 receptor and attachment of SARS-CoV-2 and human coronavirus (HCoV-NL63) to tissue sections of human trachea. These images show formalin-fixed paraffin-embedded cross-sections of human tracheal sections stained with ImmPRESS VR anti-mouse/rabbit IgG horseradish peroxidase (HRP) polymer detection kit. Dark brown represents the presence of a protein interacting with a specific antibody and is considered a positive expression. Pale brown background, and the nucleus counterstained with hematoxylin is blue. ( a ) Expression of ACE2 in human trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL); and ( d ) corresponding negative control tissue sections stained with secondary anti-mouse HRP antibody only. ( b , c , e , f ) The tissues sections presented in this panel show virus binding in formalin-fixed paraffin-embedded cross-sections after overnight incubation with 250 μL ( b ) heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 or ( c ) HCoV-NL63. ( e , f ) Virus-incubated sections that were stained with secondary anti-rabbit HRP antibody only. Scale bar—100 μm.
Figure Legend Snippet: Distribution of ACE2 receptor and attachment of SARS-CoV-2 and human coronavirus (HCoV-NL63) to tissue sections of human trachea. These images show formalin-fixed paraffin-embedded cross-sections of human tracheal sections stained with ImmPRESS VR anti-mouse/rabbit IgG horseradish peroxidase (HRP) polymer detection kit. Dark brown represents the presence of a protein interacting with a specific antibody and is considered a positive expression. Pale brown background, and the nucleus counterstained with hematoxylin is blue. ( a ) Expression of ACE2 in human trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL); and ( d ) corresponding negative control tissue sections stained with secondary anti-mouse HRP antibody only. ( b , c , e , f ) The tissues sections presented in this panel show virus binding in formalin-fixed paraffin-embedded cross-sections after overnight incubation with 250 μL ( b ) heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 or ( c ) HCoV-NL63. ( e , f ) Virus-incubated sections that were stained with secondary anti-rabbit HRP antibody only. Scale bar—100 μm.

Techniques Used: Formalin-fixed Paraffin-Embedded, Staining, Expressing, Immunostaining, Negative Control, Binding Assay, Incubation

Characterization of human respiratory epithelial cells (HRECs) for pan-cytokeratin and angiotensin-converting enzyme-2 (ACE2) using target-specific markers. Primary HRECs stained for mouse monoclonal to anti-pan-cytokeratin (epithelial cell marker; 0.5 μg/mL) showed strong expression both in ( a ) IHC and ( c ) flow cytometry analysis. ( b ) HRECs incubated with a secondary antibody only displayed minimum background. ( c ) HRECs were also quantified for the ACE2 expression using flow cytometry. Flow cytometry data was collected using an Attune NxT flow cytometer. A representative of 10,000 events were acquired and analyzed for each sample. Cells were gated for singlet population using forward (FSA) and side-scatter (SSA) properties, and the mean of percent live cell population was used to quantify the levels of ( c-i ) pan-cytokeratin and ( c-ii ) ACE2 ( n = 4). The bar graph represents the mean with the standard error of the mean (SEM).
Figure Legend Snippet: Characterization of human respiratory epithelial cells (HRECs) for pan-cytokeratin and angiotensin-converting enzyme-2 (ACE2) using target-specific markers. Primary HRECs stained for mouse monoclonal to anti-pan-cytokeratin (epithelial cell marker; 0.5 μg/mL) showed strong expression both in ( a ) IHC and ( c ) flow cytometry analysis. ( b ) HRECs incubated with a secondary antibody only displayed minimum background. ( c ) HRECs were also quantified for the ACE2 expression using flow cytometry. Flow cytometry data was collected using an Attune NxT flow cytometer. A representative of 10,000 events were acquired and analyzed for each sample. Cells were gated for singlet population using forward (FSA) and side-scatter (SSA) properties, and the mean of percent live cell population was used to quantify the levels of ( c-i ) pan-cytokeratin and ( c-ii ) ACE2 ( n = 4). The bar graph represents the mean with the standard error of the mean (SEM).

Techniques Used: Staining, Marker, Expressing, Flow Cytometry, Incubation


Structured Review

Santa Cruz Biotechnology mouse monoclonal anti ace2 e11 isotype igg1
Mouse Monoclonal Anti Ace2 E11 Isotype Igg1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti ace2 e11 isotype igg1/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal anti ace2 e11 isotype igg1 - by Bioz Stars, 2024-06
86/100 stars

Images


Structured Review

Santa Cruz Biotechnology mouse monoclonal anti ace2
Mouse Monoclonal Anti Ace2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti ace2/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal anti ace2 - by Bioz Stars, 2024-06
86/100 stars

Images

mouse anti human ace2 monoclonal antibody conjugated to af 647  (Santa Cruz Biotechnology)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af 647
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af 647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af 647/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af 647 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    mouse anti human ace2 monoclonal antibody conjugated to af 647  (Santa Cruz Biotechnology)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af 647
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af 647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af 647/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af 647 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    mouse anti human ace2 monoclonal antibody conjugated to af 647  (Santa Cruz Biotechnology)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af 647
    Expression of <t>ACE2</t> on the surface of HEK-293 T, Caco-2, Vero, and Vero E6 cells. ( A ) Proportion of cells expressing ACE2 ( B ) Relative expression of ACE2 on cell surface as expressed by median fluorescence intensity (MFI) of ACE2-AF647. Average of 3 separate experiments done in duplicate with standard deviations shown. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af 647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af 647/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af 647 - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-concept"

    Article Title: A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-concept

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-22921-7

    Expression of ACE2 on the surface of HEK-293 T, Caco-2, Vero, and Vero E6 cells. ( A ) Proportion of cells expressing ACE2 ( B ) Relative expression of ACE2 on cell surface as expressed by median fluorescence intensity (MFI) of ACE2-AF647. Average of 3 separate experiments done in duplicate with standard deviations shown. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).
    Figure Legend Snippet: Expression of ACE2 on the surface of HEK-293 T, Caco-2, Vero, and Vero E6 cells. ( A ) Proportion of cells expressing ACE2 ( B ) Relative expression of ACE2 on cell surface as expressed by median fluorescence intensity (MFI) of ACE2-AF647. Average of 3 separate experiments done in duplicate with standard deviations shown. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).

    Techniques Used: Expressing, Fluorescence

    mouse anti human ace2 monoclonal antibody conjugated to af 647  (Santa Cruz Biotechnology)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af 647
    Expression of <t>ACE2</t> on the surface of HEK-293 T, Caco-2, Vero, and Vero E6 cells. ( A ) Proportion of cells expressing ACE2 ( B ) Relative expression of ACE2 on cell surface as expressed by median fluorescence intensity (MFI) of ACE2-AF647. Average of 3 separate experiments done in duplicate with standard deviations shown. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af 647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af 647/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af 647 - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-concept"

    Article Title: A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-concept

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-22921-7

    Expression of ACE2 on the surface of HEK-293 T, Caco-2, Vero, and Vero E6 cells. ( A ) Proportion of cells expressing ACE2 ( B ) Relative expression of ACE2 on cell surface as expressed by median fluorescence intensity (MFI) of ACE2-AF647. Average of 3 separate experiments done in duplicate with standard deviations shown. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).
    Figure Legend Snippet: Expression of ACE2 on the surface of HEK-293 T, Caco-2, Vero, and Vero E6 cells. ( A ) Proportion of cells expressing ACE2 ( B ) Relative expression of ACE2 on cell surface as expressed by median fluorescence intensity (MFI) of ACE2-AF647. Average of 3 separate experiments done in duplicate with standard deviations shown. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).

    Techniques Used: Expressing, Fluorescence

    mouse anti human ace2 monoclonal antibody conjugated to af  (Santa Cruz Biotechnology)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af - by Bioz Stars, 2024-06
    96/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Santa Cruz Biotechnology monoclonal anti ace2 mouse igg
    Morphological analysis and development of BEAS-CTL and <t>BEAS-ACE2</t> spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
    Monoclonal Anti Ace2 Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti ace2 mouse igg/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti ace2 mouse igg - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology mouse monoclonal anti ace2
    Morphological analysis and development of BEAS-CTL and <t>BEAS-ACE2</t> spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
    Mouse Monoclonal Anti Ace2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti ace2/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti ace2 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology mouse monoclonal anti ace2 e11 isotype igg1
    Morphological analysis and development of BEAS-CTL and <t>BEAS-ACE2</t> spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
    Mouse Monoclonal Anti Ace2 E11 Isotype Igg1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti ace2 e11 isotype igg1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti ace2 e11 isotype igg1 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af 647
    Morphological analysis and development of BEAS-CTL and <t>BEAS-ACE2</t> spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af 647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af 647/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af 647 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology mouse anti human ace2 monoclonal antibody conjugated to af
    Morphological analysis and development of BEAS-CTL and <t>BEAS-ACE2</t> spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.
    Mouse Anti Human Ace2 Monoclonal Antibody Conjugated To Af, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ace2 monoclonal antibody conjugated to af/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human ace2 monoclonal antibody conjugated to af - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    Morphological analysis and development of BEAS-CTL and BEAS-ACE2 spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.

    Journal: Biomaterials and Biosystems

    Article Title: Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection

    doi: 10.1016/j.bbiosy.2023.100082

    Figure Lengend Snippet: Morphological analysis and development of BEAS-CTL and BEAS-ACE2 spheroids and 2D cells . (A) Scaffold-free spheroid-producing technique using agarose micro-molds. (B) Bright-field images of the spheroids on days 1, 7, and 14. (C) Spheroids historesin assay. Comparison of spheroids’ (D) diameter and (E) volume. (F) Bright-field images of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. (G) Area measurement of 2D cultures of BEAS-CTL and BEAS-ACE2 cells. Scale bar: 50-100 µm. *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. n = 25-50.

    Article Snippet: Then, they were resuspended in TBSTX (TBS [pure Tris base (Trimethamine, Bio-Rad, Brazil) + NaCl] + Triton [Triton X-100 detergent, Bio-Rad, Brazil]), and later, incubated with primary antibodies (Polyclonal Anti-Spike rabbit IgG [Anti-SARS-CoV-2 spike glycoprotein antibody – Coronavirus (ab272504), Abcam, United States] and Monoclonal Anti-ACE2 mouse IgG [ACE2 antibody (E-11) - sc-390851, Santa Cruz Animal Health, Brazil]) overnight at 4°C.

    Techniques:

    MatriWells representative scheme and SARS-CoV-2 kinetics in BEAS-ACE2 MatriWells and HUVEC cells . (A) Pulmonary ALI creation using the MatriWells, BEAS-ACE2 and HUVEC cells. (B) Intracellular viral load in BEAS-ACE2 MatriWells. (C) Number of viral particles per mL(PFU[plaque-forming units]/mL) in HUVEC's supernatant. (D) Intracellular viral load in HUVEC cells. *p ≤ 0.05; ***p ≤ 0.001. n = 12.

    Journal: Biomaterials and Biosystems

    Article Title: Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection

    doi: 10.1016/j.bbiosy.2023.100082

    Figure Lengend Snippet: MatriWells representative scheme and SARS-CoV-2 kinetics in BEAS-ACE2 MatriWells and HUVEC cells . (A) Pulmonary ALI creation using the MatriWells, BEAS-ACE2 and HUVEC cells. (B) Intracellular viral load in BEAS-ACE2 MatriWells. (C) Number of viral particles per mL(PFU[plaque-forming units]/mL) in HUVEC's supernatant. (D) Intracellular viral load in HUVEC cells. *p ≤ 0.05; ***p ≤ 0.001. n = 12.

    Article Snippet: Then, they were resuspended in TBSTX (TBS [pure Tris base (Trimethamine, Bio-Rad, Brazil) + NaCl] + Triton [Triton X-100 detergent, Bio-Rad, Brazil]), and later, incubated with primary antibodies (Polyclonal Anti-Spike rabbit IgG [Anti-SARS-CoV-2 spike glycoprotein antibody – Coronavirus (ab272504), Abcam, United States] and Monoclonal Anti-ACE2 mouse IgG [ACE2 antibody (E-11) - sc-390851, Santa Cruz Animal Health, Brazil]) overnight at 4°C.

    Techniques:

    BEAS-CTL and BEAS-ACE2 spheroids viability over time . The Viability over time was quantified using the trypan blue staining. *p ≤ 0.05, n = 3.

    Journal: Biomaterials and Biosystems

    Article Title: Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection

    doi: 10.1016/j.bbiosy.2023.100082

    Figure Lengend Snippet: BEAS-CTL and BEAS-ACE2 spheroids viability over time . The Viability over time was quantified using the trypan blue staining. *p ≤ 0.05, n = 3.

    Article Snippet: Then, they were resuspended in TBSTX (TBS [pure Tris base (Trimethamine, Bio-Rad, Brazil) + NaCl] + Triton [Triton X-100 detergent, Bio-Rad, Brazil]), and later, incubated with primary antibodies (Polyclonal Anti-Spike rabbit IgG [Anti-SARS-CoV-2 spike glycoprotein antibody – Coronavirus (ab272504), Abcam, United States] and Monoclonal Anti-ACE2 mouse IgG [ACE2 antibody (E-11) - sc-390851, Santa Cruz Animal Health, Brazil]) overnight at 4°C.

    Techniques: Staining

    Viral load quantification and immunofluorescence assay of BEAS-CTL and BEAS-ACE2 spheroids . (A) Spheroid intracellular viral concentration. (B) Immunofluorescence microscopy images of the spheroids with nucleus (Hoechst), ACE2, and Spike protein staining. Fluorescence quantification of (C) Spike protein and (D) ACE2 expression. (E) Pearson correlation comparing ACE2 and Spike protein expression. Scale bar: 50 µm. *p ≤ 0.05; **p ≤ 0.01. n = 3-5

    Journal: Biomaterials and Biosystems

    Article Title: Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection

    doi: 10.1016/j.bbiosy.2023.100082

    Figure Lengend Snippet: Viral load quantification and immunofluorescence assay of BEAS-CTL and BEAS-ACE2 spheroids . (A) Spheroid intracellular viral concentration. (B) Immunofluorescence microscopy images of the spheroids with nucleus (Hoechst), ACE2, and Spike protein staining. Fluorescence quantification of (C) Spike protein and (D) ACE2 expression. (E) Pearson correlation comparing ACE2 and Spike protein expression. Scale bar: 50 µm. *p ≤ 0.05; **p ≤ 0.01. n = 3-5

    Article Snippet: Then, they were resuspended in TBSTX (TBS [pure Tris base (Trimethamine, Bio-Rad, Brazil) + NaCl] + Triton [Triton X-100 detergent, Bio-Rad, Brazil]), and later, incubated with primary antibodies (Polyclonal Anti-Spike rabbit IgG [Anti-SARS-CoV-2 spike glycoprotein antibody – Coronavirus (ab272504), Abcam, United States] and Monoclonal Anti-ACE2 mouse IgG [ACE2 antibody (E-11) - sc-390851, Santa Cruz Animal Health, Brazil]) overnight at 4°C.

    Techniques: Immunofluorescence, Concentration Assay, Microscopy, Staining, Fluorescence, Expressing

    SARS-CoV-2 infection of the 3D models . (A) SARS-CoV-2 infection of spheroids and MatriWells. (B) Comparison of intracellular viral load in BEAS-CTL spheroids and MatriWells. (C) Comparison of intracellular viral load in BEAS-ACE2 spheroids and MatriWells. ****p ≤ 0.0001, *p≤0.05. n = 5-15

    Journal: Biomaterials and Biosystems

    Article Title: Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection

    doi: 10.1016/j.bbiosy.2023.100082

    Figure Lengend Snippet: SARS-CoV-2 infection of the 3D models . (A) SARS-CoV-2 infection of spheroids and MatriWells. (B) Comparison of intracellular viral load in BEAS-CTL spheroids and MatriWells. (C) Comparison of intracellular viral load in BEAS-ACE2 spheroids and MatriWells. ****p ≤ 0.0001, *p≤0.05. n = 5-15

    Article Snippet: Then, they were resuspended in TBSTX (TBS [pure Tris base (Trimethamine, Bio-Rad, Brazil) + NaCl] + Triton [Triton X-100 detergent, Bio-Rad, Brazil]), and later, incubated with primary antibodies (Polyclonal Anti-Spike rabbit IgG [Anti-SARS-CoV-2 spike glycoprotein antibody – Coronavirus (ab272504), Abcam, United States] and Monoclonal Anti-ACE2 mouse IgG [ACE2 antibody (E-11) - sc-390851, Santa Cruz Animal Health, Brazil]) overnight at 4°C.

    Techniques: Infection