mouse monoclonal anti βactin  (Thermo Fisher)


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    Thermo Fisher mouse monoclonal anti βactin
    Western blots of secreted ( a ) and intracellular ( b ) protein fractions of Expi293F cells expressing WT and B.1.351 RBDmfc together with increasing amounts of PDIA2. Secreted and intracellular protein fractions described in were analysed by Western blot using antibodies directed against the recombinant RBDmfc proteins (anti-RBD, anti-Fc), against chaperones <t>(anti-PDIA2,</t> <t>anti-HSPA5,</t> anti-HSP90B1), and against <t>βactin</t> as loading control. M, protein ladder (apparent MW in kDa indicated on the left); *, endogenous PDIA2 protein (anti-PDIA2 blot); arrow, RBDmfc proteins (anti-RBD and anti-Fc blots in panel ( b )) likely lacking disulphide bonds and mature glycosylations, and thus running in SDS-PAGE with an apparent MW of 50 kDa (compare to 59 kDa reduced, mature proteins shown in , lanes 3 and 4). Quantification of signal intensity of chaperones is shown in . Data are from one representative experiment (out of three independent experiments). Uncropped blot images are shown in and .
    Mouse Monoclonal Anti βactin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti βactin/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti βactin - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian Cells"

    Article Title: Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian Cells

    Journal: Biomolecules

    doi: 10.3390/biom12091170

    Western blots of secreted ( a ) and intracellular ( b ) protein fractions of Expi293F cells expressing WT and B.1.351 RBDmfc together with increasing amounts of PDIA2. Secreted and intracellular protein fractions described in were analysed by Western blot using antibodies directed against the recombinant RBDmfc proteins (anti-RBD, anti-Fc), against chaperones (anti-PDIA2, anti-HSPA5, anti-HSP90B1), and against βactin as loading control. M, protein ladder (apparent MW in kDa indicated on the left); *, endogenous PDIA2 protein (anti-PDIA2 blot); arrow, RBDmfc proteins (anti-RBD and anti-Fc blots in panel ( b )) likely lacking disulphide bonds and mature glycosylations, and thus running in SDS-PAGE with an apparent MW of 50 kDa (compare to 59 kDa reduced, mature proteins shown in , lanes 3 and 4). Quantification of signal intensity of chaperones is shown in . Data are from one representative experiment (out of three independent experiments). Uncropped blot images are shown in and .
    Figure Legend Snippet: Western blots of secreted ( a ) and intracellular ( b ) protein fractions of Expi293F cells expressing WT and B.1.351 RBDmfc together with increasing amounts of PDIA2. Secreted and intracellular protein fractions described in were analysed by Western blot using antibodies directed against the recombinant RBDmfc proteins (anti-RBD, anti-Fc), against chaperones (anti-PDIA2, anti-HSPA5, anti-HSP90B1), and against βactin as loading control. M, protein ladder (apparent MW in kDa indicated on the left); *, endogenous PDIA2 protein (anti-PDIA2 blot); arrow, RBDmfc proteins (anti-RBD and anti-Fc blots in panel ( b )) likely lacking disulphide bonds and mature glycosylations, and thus running in SDS-PAGE with an apparent MW of 50 kDa (compare to 59 kDa reduced, mature proteins shown in , lanes 3 and 4). Quantification of signal intensity of chaperones is shown in . Data are from one representative experiment (out of three independent experiments). Uncropped blot images are shown in and .

    Techniques Used: Western Blot, Expressing, Recombinant, SDS Page

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