mouse anti human aat monoclonal polymer protein recognize antibody  (Hycult Biotech)


Bioz Verified Symbol Hycult Biotech is a verified supplier
Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Hycult Biotech mouse anti human aat monoclonal polymer protein recognize antibody
    Mouse Anti Human Aat Monoclonal Polymer Protein Recognize Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human aat monoclonal polymer protein recognize antibody/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human aat monoclonal polymer protein recognize antibody - by Bioz Stars, 2024-07
    93/100 stars

    Images

    mouse anti human aat monoclonal polymer protein recognize antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Hycult Biotech mouse anti human aat monoclonal polymer protein recognize antibody
    Mouse Anti Human Aat Monoclonal Polymer Protein Recognize Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human aat monoclonal polymer protein recognize antibody/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human aat monoclonal polymer protein recognize antibody - by Bioz Stars, 2024-07
    93/100 stars

    Images

    mouse monoclonal anti aat polymers  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Hycult Biotech mouse monoclonal anti aat polymers
    (A) Targeting strategy for the SERPINA1 <t>(AAT)</t> locus. (B) Schematic of directed differentiation protocol for generating iHeps. (C) Representative flow cytometry plots of fixed, permeabilized ZZ, MZ, and MM iHeps. (D) MFI of intracellular AAT protein in ZZ, MZ and MM iHeps. (E and F) Immunostaining of ZZ, MZ, and MM iHeps for AAT, <t>2C1,</t> and HNF4α; scale bar, 10 μm. (G and H) Secreted total (G) AAT and (H) ZAAT protein in ZZ, MZ, and MM iHep supernatants. (I) Assay of anti-neutrophil elastase inhibition in concentrated iHep supernatants. (J) Representative quantification of AAT secretion kinetics using 35 S-Met/Cys-labeled AAT protein from intracellular protein lysates and supernatants. (K) Kinetic of aggregated AAT-labeled cell lysate and supernatants from (J). n = 3 independent experiments from each of the syngeneic backgrounds (D and G–I). n = 1 independent experiment from each of the syngeneic backgrounds (K). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test using MZ as control. Neutrophil elastase inhibition ZZ versus MZ **p < 0.01, ZZ versus MM ### p < 0.001, MZ versus MM $$$ p < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.
    Mouse Monoclonal Anti Aat Polymers, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymers/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymers - by Bioz Stars, 2024-07
    93/100 stars

    Images

    1) Product Images from "Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity"

    Article Title: Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity

    Journal: Cell reports

    doi: 10.1016/j.celrep.2022.111775

    (A) Targeting strategy for the SERPINA1 (AAT) locus. (B) Schematic of directed differentiation protocol for generating iHeps. (C) Representative flow cytometry plots of fixed, permeabilized ZZ, MZ, and MM iHeps. (D) MFI of intracellular AAT protein in ZZ, MZ and MM iHeps. (E and F) Immunostaining of ZZ, MZ, and MM iHeps for AAT, 2C1, and HNF4α; scale bar, 10 μm. (G and H) Secreted total (G) AAT and (H) ZAAT protein in ZZ, MZ, and MM iHep supernatants. (I) Assay of anti-neutrophil elastase inhibition in concentrated iHep supernatants. (J) Representative quantification of AAT secretion kinetics using 35 S-Met/Cys-labeled AAT protein from intracellular protein lysates and supernatants. (K) Kinetic of aggregated AAT-labeled cell lysate and supernatants from (J). n = 3 independent experiments from each of the syngeneic backgrounds (D and G–I). n = 1 independent experiment from each of the syngeneic backgrounds (K). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test using MZ as control. Neutrophil elastase inhibition ZZ versus MZ **p < 0.01, ZZ versus MM ### p < 0.001, MZ versus MM $$$ p < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.
    Figure Legend Snippet: (A) Targeting strategy for the SERPINA1 (AAT) locus. (B) Schematic of directed differentiation protocol for generating iHeps. (C) Representative flow cytometry plots of fixed, permeabilized ZZ, MZ, and MM iHeps. (D) MFI of intracellular AAT protein in ZZ, MZ and MM iHeps. (E and F) Immunostaining of ZZ, MZ, and MM iHeps for AAT, 2C1, and HNF4α; scale bar, 10 μm. (G and H) Secreted total (G) AAT and (H) ZAAT protein in ZZ, MZ, and MM iHep supernatants. (I) Assay of anti-neutrophil elastase inhibition in concentrated iHep supernatants. (J) Representative quantification of AAT secretion kinetics using 35 S-Met/Cys-labeled AAT protein from intracellular protein lysates and supernatants. (K) Kinetic of aggregated AAT-labeled cell lysate and supernatants from (J). n = 3 independent experiments from each of the syngeneic backgrounds (D and G–I). n = 1 independent experiment from each of the syngeneic backgrounds (K). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test using MZ as control. Neutrophil elastase inhibition ZZ versus MZ **p < 0.01, ZZ versus MM ### p < 0.001, MZ versus MM $$$ p < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.

    Techniques Used: Flow Cytometry, Immunostaining, Inhibition, Labeling


    Figure Legend Snippet:

    Techniques Used: Recombinant, Electron Microscopy, Lysis, Lactate Assay, Pyruvate Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Sequencing, Mutagenesis, Software, Modification

    mouse monoclonal anti aat polymer antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Hycult Biotech mouse monoclonal anti aat polymer antibody
    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma <t>Z-AAT</t> polymers (µg/mL) were measured by ELISA based on <t>LG96</t> mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.
    Mouse Monoclonal Anti Aat Polymer Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymer antibody/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymer antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "The Relationship between Plasma Alpha-1-Antitrypsin Polymers and Lung or Liver Function in ZZ Alpha-1-Antitrypsin-Deficient Patients"

    Article Title: The Relationship between Plasma Alpha-1-Antitrypsin Polymers and Lung or Liver Function in ZZ Alpha-1-Antitrypsin-Deficient Patients

    Journal: Biomolecules

    doi: 10.3390/biom12030380

    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma Z-AAT polymers (µg/mL) were measured by ELISA based on LG96 mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.
    Figure Legend Snippet: Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma Z-AAT polymers (µg/mL) were measured by ELISA based on LG96 mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.

    Techniques Used: Diffusion-based Assay, Enzyme-linked Immunosorbent Assay

    Z-AAT polymer analysis by Western blots using two different monoclonal antibodies. Equal amounts of plasma samples from seven ZZ-AATD individuals were electrophoretically separated using 7.5% native polyacrylamide gels followed by western blots. Purified polymeric Z-AAT isolated from pooled ZZ plasma samples was used as a positive control. The western blots were probed against mouse monoclonal anti-AAT polymer antibodies LG96 ( A ) and 2C1 ( B )(both diluted 1:700). The image demonstrates that both antibodies recognize the Z-AAT polymers, but the profiles of recognized polymers differ. This blot is the representative from n = 2 independent repeats.
    Figure Legend Snippet: Z-AAT polymer analysis by Western blots using two different monoclonal antibodies. Equal amounts of plasma samples from seven ZZ-AATD individuals were electrophoretically separated using 7.5% native polyacrylamide gels followed by western blots. Purified polymeric Z-AAT isolated from pooled ZZ plasma samples was used as a positive control. The western blots were probed against mouse monoclonal anti-AAT polymer antibodies LG96 ( A ) and 2C1 ( B )(both diluted 1:700). The image demonstrates that both antibodies recognize the Z-AAT polymers, but the profiles of recognized polymers differ. This blot is the representative from n = 2 independent repeats.

    Techniques Used: Western Blot, Purification, Isolation, Positive Control

    mouse monoclonal anti aat polymer antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Hycult Biotech mouse monoclonal anti aat polymer antibody
    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma <t>Z-AAT</t> polymers (µg/mL) were measured by ELISA based on <t>LG96</t> mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.
    Mouse Monoclonal Anti Aat Polymer Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymer antibody/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymer antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "The Relationship between Plasma Alpha-1-Antitrypsin Polymers and Lung or Liver Function in ZZ Alpha-1-Antitrypsin-Deficient Patients"

    Article Title: The Relationship between Plasma Alpha-1-Antitrypsin Polymers and Lung or Liver Function in ZZ Alpha-1-Antitrypsin-Deficient Patients

    Journal: Biomolecules

    doi: 10.3390/biom12030380

    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma Z-AAT polymers (µg/mL) were measured by ELISA based on LG96 mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.
    Figure Legend Snippet: Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma Z-AAT polymers (µg/mL) were measured by ELISA based on LG96 mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.

    Techniques Used: Diffusion-based Assay, Enzyme-linked Immunosorbent Assay

    Z-AAT polymer analysis by Western blots using two different monoclonal antibodies. Equal amounts of plasma samples from seven ZZ-AATD individuals were electrophoretically separated using 7.5% native polyacrylamide gels followed by western blots. Purified polymeric Z-AAT isolated from pooled ZZ plasma samples was used as a positive control. The western blots were probed against mouse monoclonal anti-AAT polymer antibodies LG96 ( A ) and 2C1 ( B )(both diluted 1:700). The image demonstrates that both antibodies recognize the Z-AAT polymers, but the profiles of recognized polymers differ. This blot is the representative from n = 2 independent repeats.
    Figure Legend Snippet: Z-AAT polymer analysis by Western blots using two different monoclonal antibodies. Equal amounts of plasma samples from seven ZZ-AATD individuals were electrophoretically separated using 7.5% native polyacrylamide gels followed by western blots. Purified polymeric Z-AAT isolated from pooled ZZ plasma samples was used as a positive control. The western blots were probed against mouse monoclonal anti-AAT polymer antibodies LG96 ( A ) and 2C1 ( B )(both diluted 1:700). The image demonstrates that both antibodies recognize the Z-AAT polymers, but the profiles of recognized polymers differ. This blot is the representative from n = 2 independent repeats.

    Techniques Used: Western Blot, Purification, Isolation, Positive Control

    mouse monoclonal anti aat polymer antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Hycult Biotech mouse monoclonal anti aat polymer antibody
    <t>CX3CR1</t> gene expression levels in peripheral blood mononuclear cells (PBMCs) related to alpha-1 antitrypsin-deficiency (AATD) and plasma concentrations of CX3CR1 ligand (CX3CL1) and <t>Z-AAT</t> polymer. ( A )PBMCs were isolated from AATD subjects and non-AATD controls. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Measurements were carried out in duplicates. Data are presented as median (IQR) in boxplots, lines represent medians. Outliers are defined as data points located outside the whiskers. p-Value was calculated by Mann-Whitney U test. ( B ) Plasma levels of CX3CL1 in AATD (plasma available for n = 38 AATD) and non-AATD individuals measured by ELISA. Measurements were carried out in triplicates. Data are presented as median (IQR) in boxplots with whiskers. Outliers are defined as data points located outside the whiskers. ( C ) Negative correlation of CX3CR1 mRNA in PBMCs and plasma Z-AAT polymer levels from ZZ AATD individuals from graph ( B ). Pearson’s correlation test, r 2 = −0.313, p=0.055, n = 38. Figure 1—source data 1. Source files, containing original data for , to document CX3CR1 expression ( A ), and plasma levels of CX3CL1 in alpha-1 antitrypsin-deficient (AATD) and non-AATD individuals ( B ).
    Mouse Monoclonal Anti Aat Polymer Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymer antibody/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymer antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs"

    Article Title: Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs

    Journal: eLife

    doi: 10.7554/eLife.64881

    CX3CR1 gene expression levels in peripheral blood mononuclear cells (PBMCs) related to alpha-1 antitrypsin-deficiency (AATD) and plasma concentrations of CX3CR1 ligand (CX3CL1) and Z-AAT polymer. ( A )PBMCs were isolated from AATD subjects and non-AATD controls. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Measurements were carried out in duplicates. Data are presented as median (IQR) in boxplots, lines represent medians. Outliers are defined as data points located outside the whiskers. p-Value was calculated by Mann-Whitney U test. ( B ) Plasma levels of CX3CL1 in AATD (plasma available for n = 38 AATD) and non-AATD individuals measured by ELISA. Measurements were carried out in triplicates. Data are presented as median (IQR) in boxplots with whiskers. Outliers are defined as data points located outside the whiskers. ( C ) Negative correlation of CX3CR1 mRNA in PBMCs and plasma Z-AAT polymer levels from ZZ AATD individuals from graph ( B ). Pearson’s correlation test, r 2 = −0.313, p=0.055, n = 38. Figure 1—source data 1. Source files, containing original data for , to document CX3CR1 expression ( A ), and plasma levels of CX3CL1 in alpha-1 antitrypsin-deficient (AATD) and non-AATD individuals ( B ).
    Figure Legend Snippet: CX3CR1 gene expression levels in peripheral blood mononuclear cells (PBMCs) related to alpha-1 antitrypsin-deficiency (AATD) and plasma concentrations of CX3CR1 ligand (CX3CL1) and Z-AAT polymer. ( A )PBMCs were isolated from AATD subjects and non-AATD controls. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Measurements were carried out in duplicates. Data are presented as median (IQR) in boxplots, lines represent medians. Outliers are defined as data points located outside the whiskers. p-Value was calculated by Mann-Whitney U test. ( B ) Plasma levels of CX3CL1 in AATD (plasma available for n = 38 AATD) and non-AATD individuals measured by ELISA. Measurements were carried out in triplicates. Data are presented as median (IQR) in boxplots with whiskers. Outliers are defined as data points located outside the whiskers. ( C ) Negative correlation of CX3CR1 mRNA in PBMCs and plasma Z-AAT polymer levels from ZZ AATD individuals from graph ( B ). Pearson’s correlation test, r 2 = −0.313, p=0.055, n = 38. Figure 1—source data 1. Source files, containing original data for , to document CX3CR1 expression ( A ), and plasma levels of CX3CL1 in alpha-1 antitrypsin-deficient (AATD) and non-AATD individuals ( B ).

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay

    PBMCs were incubated for 18 hr with plasma-derived Z-AAT in the concentrations as indicated. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves show results from two independent experiments. Each point represents mean of two repeats.
    Figure Legend Snippet: PBMCs were incubated for 18 hr with plasma-derived Z-AAT in the concentrations as indicated. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves show results from two independent experiments. Each point represents mean of two repeats.

    Techniques Used: Incubation, Derivative Assay, Expressing, Real-time Polymerase Chain Reaction

    ( A ) CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Peripheral blood mononuclear cells (PBMCs) were incubated for 18 hr with plasma-derived Z-AAT, lipopolysaccharide (LPS), or M-AAT in the concentrations as indicated, or with RPMI medium alone (control). The data from n = 6 independent experiments are presented as median (IQR) in box and whisker plot format; lines represent medians in each box. Measurements were carried out in duplicates. p-Value was calculated by nonparametric Kruskal-Wallis test. ( B ) Representative uncut Western blot (n = 3 independent experiments) of CX3CR1 in RIPA lysates prepared from PBMCs incubated for 18 hr alone or with LPS (1 µg/ml), M-AAT (1 mg/ml), or Z-AAT (0.5 mg/ml). For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. Relative intensities were calculated for each band using the ratio relative to β-actin, as a loading control, and then normalized by the experimental control. ( C ) For analysis of cellular AAT, the same lysates were separated under non-reducing conditions. Western blots were probed with polyclonal rabbit anti-human AAT recognizing monomeric, polymeric, or truncated forms of AAT. One representative blot from n = 3 independent experiments is shown. β-Actin was used for a loading control. ( D ) and ( E ) Co-distribution of Z-AAT polymers with CX3CR1 in human total PBMCs incubated with 0.5 mg/ml Z-AAT polymers for 18 hr. ( D ) Immunofluorescence microscopy revealed co-localization of Z-AAT polymers ( red ) with CX3CR1-positive structures ( green ). Arrows point areas of co-localization. Scale bar, 10 µm. ( E ) Confocal microscopy 3D stack with orthogonal reconstruction shows an aggregate of Z-AAT polymers ( red ) surrounded by CX3CR1-positive ( green ) cellular extensions forming a cap-like structure (arrowhead). Scale bar, 5 µm. The images with indicated channels merged and the corresponding differential interference contrast (DIC) image are presented. 4', 6- Diamidino 2-phenylindole (DAPI) was used for nuclei staining ( blue ). Figure 2—source data 1. Source file, containing original data for , to document reduced CX3CR1 expression in peripheral blood mononuclear cells (PBMCs) treated with Z alpha-1 antitrypsin (Z-AAT) or lipopolysaccharide (LPS) ( A ).
    Figure Legend Snippet: ( A ) CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Peripheral blood mononuclear cells (PBMCs) were incubated for 18 hr with plasma-derived Z-AAT, lipopolysaccharide (LPS), or M-AAT in the concentrations as indicated, or with RPMI medium alone (control). The data from n = 6 independent experiments are presented as median (IQR) in box and whisker plot format; lines represent medians in each box. Measurements were carried out in duplicates. p-Value was calculated by nonparametric Kruskal-Wallis test. ( B ) Representative uncut Western blot (n = 3 independent experiments) of CX3CR1 in RIPA lysates prepared from PBMCs incubated for 18 hr alone or with LPS (1 µg/ml), M-AAT (1 mg/ml), or Z-AAT (0.5 mg/ml). For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. Relative intensities were calculated for each band using the ratio relative to β-actin, as a loading control, and then normalized by the experimental control. ( C ) For analysis of cellular AAT, the same lysates were separated under non-reducing conditions. Western blots were probed with polyclonal rabbit anti-human AAT recognizing monomeric, polymeric, or truncated forms of AAT. One representative blot from n = 3 independent experiments is shown. β-Actin was used for a loading control. ( D ) and ( E ) Co-distribution of Z-AAT polymers with CX3CR1 in human total PBMCs incubated with 0.5 mg/ml Z-AAT polymers for 18 hr. ( D ) Immunofluorescence microscopy revealed co-localization of Z-AAT polymers ( red ) with CX3CR1-positive structures ( green ). Arrows point areas of co-localization. Scale bar, 10 µm. ( E ) Confocal microscopy 3D stack with orthogonal reconstruction shows an aggregate of Z-AAT polymers ( red ) surrounded by CX3CR1-positive ( green ) cellular extensions forming a cap-like structure (arrowhead). Scale bar, 5 µm. The images with indicated channels merged and the corresponding differential interference contrast (DIC) image are presented. 4', 6- Diamidino 2-phenylindole (DAPI) was used for nuclei staining ( blue ). Figure 2—source data 1. Source file, containing original data for , to document reduced CX3CR1 expression in peripheral blood mononuclear cells (PBMCs) treated with Z alpha-1 antitrypsin (Z-AAT) or lipopolysaccharide (LPS) ( A ).

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Incubation, Derivative Assay, Whisker Assay, Western Blot, SDS Page, Immunofluorescence, Microscopy, Confocal Microscopy, Staining

    CX3CR1-expressing cells were found in the monocyte gate ( A ) and in the NK cell gate (CD56 lo CD16 + ) ( B ). Histograms show representative results and bars represent mean (SD) of n = 4 independent biological repeats each measured one time. After incubation with Z-AAT or LPS, monocytes and NK cells show significantly reduced CX3CR1 surface expression in comparison to untreated control cells. p-Values were calculated by one-way ANOVA. Figure 3—source data 1. Source files, containing original data for , to document reduced CX3CR1 surface expression in monocytes ( A ) and NK cells ( B ) after treatment with Z alpha-1 antitrypsin (Z-AAT) or lipopolysaccharide (LPS) ( A ).
    Figure Legend Snippet: CX3CR1-expressing cells were found in the monocyte gate ( A ) and in the NK cell gate (CD56 lo CD16 + ) ( B ). Histograms show representative results and bars represent mean (SD) of n = 4 independent biological repeats each measured one time. After incubation with Z-AAT or LPS, monocytes and NK cells show significantly reduced CX3CR1 surface expression in comparison to untreated control cells. p-Values were calculated by one-way ANOVA. Figure 3—source data 1. Source files, containing original data for , to document reduced CX3CR1 surface expression in monocytes ( A ) and NK cells ( B ) after treatment with Z alpha-1 antitrypsin (Z-AAT) or lipopolysaccharide (LPS) ( A ).

    Techniques Used: Expressing, Incubation

    Lipid rafts were solubilized from membrane fractions with UltraRIPA kit. ( a ) For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. One representative blot from n = 3 independent experiments is shown. ( b ) For analysis of lipid raft associated AAT polymers, the same samples were separated under non-reducing conditions. The Western blot was probed with monoclonal antibody (2C1) recognizing polymeric AAT. One representative blot from n = 3 independent experiments is shown.
    Figure Legend Snippet: Lipid rafts were solubilized from membrane fractions with UltraRIPA kit. ( a ) For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. One representative blot from n = 3 independent experiments is shown. ( b ) For analysis of lipid raft associated AAT polymers, the same samples were separated under non-reducing conditions. The Western blot was probed with monoclonal antibody (2C1) recognizing polymeric AAT. One representative blot from n = 3 independent experiments is shown.

    Techniques Used: SDS Page, Western Blot

    ( A ) CX3CR1 mRNA expression relative to HPRT1 was determined by real-time PCR using Taqman gene expression assays. Peripheral blood mononuclear cells (PBMCs) were incubated for 18 hr in RPMI medium alone or with addition of Z-AAT monomer or Z-AAT polymer (each 0.5 mg/ml), or lipopolysaccharide (LPS) (1 µg/ml) or native M-AAT (0.5 mg/ml). Measurements were carried out in duplicates. Data are represented as bars from four independent experiments. ( B ) Levels of CX3CR1 lysates prepared in RIPA buffer from PBMCs incubated for 18 hr with RPMI (control) and with Z-AAT monomer (0.5 mg/ml) or LPS (1 µg/ml) (used as a positive control). For analysis of CX3CR1, equal amounts of protein were separated by 10% SDS-PAGE under reducing conditions followed by Western blotting. Representative uncut Western blot (n = 2 independent experiments) is shown. β-Actin was used for a loading control. ( C ) CX3CR1 mRNA expression relative to HPRT1 was determined by real-time PCR using Taqman gene expression assays. PBMCs were incubated for 18 hr in RPMI medium alone or with different concentrations of heat-induced M-AAT polymers (60°C, for 3 hr). Measurements were carried out in duplicates. Data are represented as curves from two independent donors.
    Figure Legend Snippet: ( A ) CX3CR1 mRNA expression relative to HPRT1 was determined by real-time PCR using Taqman gene expression assays. Peripheral blood mononuclear cells (PBMCs) were incubated for 18 hr in RPMI medium alone or with addition of Z-AAT monomer or Z-AAT polymer (each 0.5 mg/ml), or lipopolysaccharide (LPS) (1 µg/ml) or native M-AAT (0.5 mg/ml). Measurements were carried out in duplicates. Data are represented as bars from four independent experiments. ( B ) Levels of CX3CR1 lysates prepared in RIPA buffer from PBMCs incubated for 18 hr with RPMI (control) and with Z-AAT monomer (0.5 mg/ml) or LPS (1 µg/ml) (used as a positive control). For analysis of CX3CR1, equal amounts of protein were separated by 10% SDS-PAGE under reducing conditions followed by Western blotting. Representative uncut Western blot (n = 2 independent experiments) is shown. β-Actin was used for a loading control. ( C ) CX3CR1 mRNA expression relative to HPRT1 was determined by real-time PCR using Taqman gene expression assays. PBMCs were incubated for 18 hr in RPMI medium alone or with different concentrations of heat-induced M-AAT polymers (60°C, for 3 hr). Measurements were carried out in duplicates. Data are represented as curves from two independent donors.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Incubation, Positive Control, SDS Page, Western Blot

    PBMCs were incubated for 18 hr with plasma-derived Z-AAT in the concentrations as indicated, or with RPMI medium alone (control). CX3CR1 and CD14 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves represent two independent experiments.
    Figure Legend Snippet: PBMCs were incubated for 18 hr with plasma-derived Z-AAT in the concentrations as indicated, or with RPMI medium alone (control). CX3CR1 and CD14 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves represent two independent experiments.

    Techniques Used: Incubation, Derivative Assay, Expressing, Real-time Polymerase Chain Reaction


    Figure Legend Snippet:

    Techniques Used: TaqMan Assay, Purification, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Recombinant, Software

    mouse monoclonal anti aat polymer antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Hycult Biotech mouse monoclonal anti aat polymer antibody
    Mouse Monoclonal Anti Aat Polymer Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymer antibody/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymer antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    mouse monoclonal anti aat polymer antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Hycult Biotech mouse monoclonal anti aat polymer antibody
    <t>A</t> <t>CX3CR1</t> gene expression in PBMCs isolated from AATD subjects and non-AATD controls. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Measurements were carried out in duplicates. Data are presented as median (IQR) in boxplots, lines represent medians. Outliers are defined as data points located outside the whiskers. p-value was calculated by the difference between the Mann-Whitney U test. B Plasma levels of CX3CL1 in AATD (plasma available for n = 38 AATD) and non-AATD individuals measured by ELISA. Measurements were carried out in triplicates. Data are presented as median (IQR) in boxplots with whiskers. Outliers are defined as data points located outside the whiskers. C Negative correlation of CX3CR1 mRNA in PBMCs and plasma <t>Z-AAT</t> polymer levels from ZZ-AATD individuals from graph ( B ). Person’s correlation test, r = -0.313, p = 0.055, n = 38.
    Mouse Monoclonal Anti Aat Polymer Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymer antibody/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymer antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Polymerization of misfolded protein lowers CX3CR1 expression in human PBMCs"

    Article Title: Polymerization of misfolded protein lowers CX3CR1 expression in human PBMCs

    Journal: bioRxiv

    doi: 10.1101/2020.11.24.395640

    A CX3CR1 gene expression in PBMCs isolated from AATD subjects and non-AATD controls. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Measurements were carried out in duplicates. Data are presented as median (IQR) in boxplots, lines represent medians. Outliers are defined as data points located outside the whiskers. p-value was calculated by the difference between the Mann-Whitney U test. B Plasma levels of CX3CL1 in AATD (plasma available for n = 38 AATD) and non-AATD individuals measured by ELISA. Measurements were carried out in triplicates. Data are presented as median (IQR) in boxplots with whiskers. Outliers are defined as data points located outside the whiskers. C Negative correlation of CX3CR1 mRNA in PBMCs and plasma Z-AAT polymer levels from ZZ-AATD individuals from graph ( B ). Person’s correlation test, r = -0.313, p = 0.055, n = 38.
    Figure Legend Snippet: A CX3CR1 gene expression in PBMCs isolated from AATD subjects and non-AATD controls. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Measurements were carried out in duplicates. Data are presented as median (IQR) in boxplots, lines represent medians. Outliers are defined as data points located outside the whiskers. p-value was calculated by the difference between the Mann-Whitney U test. B Plasma levels of CX3CL1 in AATD (plasma available for n = 38 AATD) and non-AATD individuals measured by ELISA. Measurements were carried out in triplicates. Data are presented as median (IQR) in boxplots with whiskers. Outliers are defined as data points located outside the whiskers. C Negative correlation of CX3CR1 mRNA in PBMCs and plasma Z-AAT polymer levels from ZZ-AATD individuals from graph ( B ). Person’s correlation test, r = -0.313, p = 0.055, n = 38.

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay

    Z-AAT in a concentration-dependent manner reduces CX3CR1 mRNA expression in PBMCs isolated from healthy donors. PBMCs were incubated for 18 h with plasma-derived Z-AAT in the concentrations as indicated. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves show results from two independent experiments. Each point represents mean of two repeats.
    Figure Legend Snippet: Z-AAT in a concentration-dependent manner reduces CX3CR1 mRNA expression in PBMCs isolated from healthy donors. PBMCs were incubated for 18 h with plasma-derived Z-AAT in the concentrations as indicated. CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves show results from two independent experiments. Each point represents mean of two repeats.

    Techniques Used: Concentration Assay, Expressing, Isolation, Incubation, Derivative Assay, Real-time Polymerase Chain Reaction

    Inverse effects of Z-AAT on CX3CR1 gene expression and protein expression. PBMCs were incubated for 18 h with plasma-derived Z-AAT, LPS or M-AAT in the concentrations as indicated, or with RPMI medium alone (control). A CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. The data from n = 6 independent experiments are presented as median (IQR) in box and whisker plot format; lines represent medians in each box. Measurements were carried out in duplicates. p was calculated by nonparametric Kruskal-Wallis test). B Representative uncut Western blot (n = 3 independent experiments) of CX3CR1 in RIPA lysates prepared from PBMCs incubated for 18 h alone or with LPS (1 µg/ml), M-AAT (1 mg/ml), or Z-AAT (0.5 mg/ml). For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. Relative intensities were calculated for each band using the ratio relative to β-actin, as a loading control, and then normalized by the experimental control. (Table, n = 3 independent experiments). C For analysis of cellular AAT the same lysates were separated under non-reducing conditions. Western blots were probed with polyclonal rabbit anti human AAT recognizing monomeric, polymeric, or truncated forms of AAT. One representative blot from n = 3 independent experiments is shown. β-actin was used for a loading control.
    Figure Legend Snippet: Inverse effects of Z-AAT on CX3CR1 gene expression and protein expression. PBMCs were incubated for 18 h with plasma-derived Z-AAT, LPS or M-AAT in the concentrations as indicated, or with RPMI medium alone (control). A CX3CR1 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. The data from n = 6 independent experiments are presented as median (IQR) in box and whisker plot format; lines represent medians in each box. Measurements were carried out in duplicates. p was calculated by nonparametric Kruskal-Wallis test). B Representative uncut Western blot (n = 3 independent experiments) of CX3CR1 in RIPA lysates prepared from PBMCs incubated for 18 h alone or with LPS (1 µg/ml), M-AAT (1 mg/ml), or Z-AAT (0.5 mg/ml). For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. Relative intensities were calculated for each band using the ratio relative to β-actin, as a loading control, and then normalized by the experimental control. (Table, n = 3 independent experiments). C For analysis of cellular AAT the same lysates were separated under non-reducing conditions. Western blots were probed with polyclonal rabbit anti human AAT recognizing monomeric, polymeric, or truncated forms of AAT. One representative blot from n = 3 independent experiments is shown. β-actin was used for a loading control.

    Techniques Used: Expressing, Incubation, Derivative Assay, Real-time Polymerase Chain Reaction, Whisker Assay, Western Blot, SDS Page

    Flow cytometric analysis of CX3CR1 surface expression in PBMCs after incubation with RPMI alone (control), Z-AAT or LPS for 18 h. CX3CR1 expressing cells were found in the monocyte gate ( A ) and in the NK cell gate (CD56 lo CD16 + ) ( B ). Histograms show representative results and bars represent mean (SD) of n = 4 independent biological repeats each measured one time. After incubation with Z-AAT or LPS monocytes and NK cells show significantly reduced CX3CR1 surface expression in comparison to untreated control cells. p-values were calculated by one-way ANOVA.
    Figure Legend Snippet: Flow cytometric analysis of CX3CR1 surface expression in PBMCs after incubation with RPMI alone (control), Z-AAT or LPS for 18 h. CX3CR1 expressing cells were found in the monocyte gate ( A ) and in the NK cell gate (CD56 lo CD16 + ) ( B ). Histograms show representative results and bars represent mean (SD) of n = 4 independent biological repeats each measured one time. After incubation with Z-AAT or LPS monocytes and NK cells show significantly reduced CX3CR1 surface expression in comparison to untreated control cells. p-values were calculated by one-way ANOVA.

    Techniques Used: Expressing, Incubation

    Z-AAT induces association of CX3CR1 with lipid rafts. Lipid rafts were solubilized from membrane fractions with ULTRA RIPA kit. a For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. One representative blot from n = 3 independent experiments is shown. b For analysis of lipid raft associated AAT polymers the same samples were separated under non-reducing conditions. The Western blot was probed with monoclonal antibody (2C1) recognizing polymeric AAT. One representative blot from n = 3 independent experiments is shown.
    Figure Legend Snippet: Z-AAT induces association of CX3CR1 with lipid rafts. Lipid rafts were solubilized from membrane fractions with ULTRA RIPA kit. a For analysis of CX3CR1, equal amounts of protein were separated by SDS-PAGE under reducing conditions. One representative blot from n = 3 independent experiments is shown. b For analysis of lipid raft associated AAT polymers the same samples were separated under non-reducing conditions. The Western blot was probed with monoclonal antibody (2C1) recognizing polymeric AAT. One representative blot from n = 3 independent experiments is shown.

    Techniques Used: SDS Page, Western Blot

    Inverse changes in CD14 and CX3CR1 mRNA expression in PBMCs treated with different concentrations of Z-AAT. PBMCs were incubated for 18 h with plasma-derived Z-AAT in the concentrations as indicated, or with RPMI medium alone (control). CX3CR1 and CD14 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves represent two independent experiments.
    Figure Legend Snippet: Inverse changes in CD14 and CX3CR1 mRNA expression in PBMCs treated with different concentrations of Z-AAT. PBMCs were incubated for 18 h with plasma-derived Z-AAT in the concentrations as indicated, or with RPMI medium alone (control). CX3CR1 and CD14 gene expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Curves represent two independent experiments.

    Techniques Used: Expressing, Incubation, Derivative Assay, Real-time Polymerase Chain Reaction

    igg1, mouse, mab mopc-21  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Hycult Biotech igg1, mouse, mab mopc-21
    Igg1, Mouse, Mab Mopc 21, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg1, mouse, mab mopc-21/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg1, mouse, mab mopc-21 - by Bioz Stars, 2024-07
    93/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Hycult Biotech mouse anti human aat monoclonal polymer protein recognize antibody
    Mouse Anti Human Aat Monoclonal Polymer Protein Recognize Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human aat monoclonal polymer protein recognize antibody/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human aat monoclonal polymer protein recognize antibody - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    93
    Hycult Biotech mouse monoclonal anti aat polymers
    (A) Targeting strategy for the SERPINA1 <t>(AAT)</t> locus. (B) Schematic of directed differentiation protocol for generating iHeps. (C) Representative flow cytometry plots of fixed, permeabilized ZZ, MZ, and MM iHeps. (D) MFI of intracellular AAT protein in ZZ, MZ and MM iHeps. (E and F) Immunostaining of ZZ, MZ, and MM iHeps for AAT, <t>2C1,</t> and HNF4α; scale bar, 10 μm. (G and H) Secreted total (G) AAT and (H) ZAAT protein in ZZ, MZ, and MM iHep supernatants. (I) Assay of anti-neutrophil elastase inhibition in concentrated iHep supernatants. (J) Representative quantification of AAT secretion kinetics using 35 S-Met/Cys-labeled AAT protein from intracellular protein lysates and supernatants. (K) Kinetic of aggregated AAT-labeled cell lysate and supernatants from (J). n = 3 independent experiments from each of the syngeneic backgrounds (D and G–I). n = 1 independent experiment from each of the syngeneic backgrounds (K). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test using MZ as control. Neutrophil elastase inhibition ZZ versus MZ **p < 0.01, ZZ versus MM ### p < 0.001, MZ versus MM $$$ p < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.
    Mouse Monoclonal Anti Aat Polymers, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymers/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymers - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    86
    Hycult Biotech mouse monoclonal anti aat polymer antibody
    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma <t>Z-AAT</t> polymers (µg/mL) were measured by ELISA based on <t>LG96</t> mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.
    Mouse Monoclonal Anti Aat Polymer Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti aat polymer antibody/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti aat polymer antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    93
    Hycult Biotech igg1, mouse, mab mopc-21
    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma <t>Z-AAT</t> polymers (µg/mL) were measured by ELISA based on <t>LG96</t> mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.
    Igg1, Mouse, Mab Mopc 21, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg1, mouse, mab mopc-21/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg1, mouse, mab mopc-21 - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) Targeting strategy for the SERPINA1 (AAT) locus. (B) Schematic of directed differentiation protocol for generating iHeps. (C) Representative flow cytometry plots of fixed, permeabilized ZZ, MZ, and MM iHeps. (D) MFI of intracellular AAT protein in ZZ, MZ and MM iHeps. (E and F) Immunostaining of ZZ, MZ, and MM iHeps for AAT, 2C1, and HNF4α; scale bar, 10 μm. (G and H) Secreted total (G) AAT and (H) ZAAT protein in ZZ, MZ, and MM iHep supernatants. (I) Assay of anti-neutrophil elastase inhibition in concentrated iHep supernatants. (J) Representative quantification of AAT secretion kinetics using 35 S-Met/Cys-labeled AAT protein from intracellular protein lysates and supernatants. (K) Kinetic of aggregated AAT-labeled cell lysate and supernatants from (J). n = 3 independent experiments from each of the syngeneic backgrounds (D and G–I). n = 1 independent experiment from each of the syngeneic backgrounds (K). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test using MZ as control. Neutrophil elastase inhibition ZZ versus MZ **p < 0.01, ZZ versus MM ### p < 0.001, MZ versus MM $$$ p < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.

    Journal: Cell reports

    Article Title: Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity

    doi: 10.1016/j.celrep.2022.111775

    Figure Lengend Snippet: (A) Targeting strategy for the SERPINA1 (AAT) locus. (B) Schematic of directed differentiation protocol for generating iHeps. (C) Representative flow cytometry plots of fixed, permeabilized ZZ, MZ, and MM iHeps. (D) MFI of intracellular AAT protein in ZZ, MZ and MM iHeps. (E and F) Immunostaining of ZZ, MZ, and MM iHeps for AAT, 2C1, and HNF4α; scale bar, 10 μm. (G and H) Secreted total (G) AAT and (H) ZAAT protein in ZZ, MZ, and MM iHep supernatants. (I) Assay of anti-neutrophil elastase inhibition in concentrated iHep supernatants. (J) Representative quantification of AAT secretion kinetics using 35 S-Met/Cys-labeled AAT protein from intracellular protein lysates and supernatants. (K) Kinetic of aggregated AAT-labeled cell lysate and supernatants from (J). n = 3 independent experiments from each of the syngeneic backgrounds (D and G–I). n = 1 independent experiment from each of the syngeneic backgrounds (K). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test using MZ as control. Neutrophil elastase inhibition ZZ versus MZ **p < 0.01, ZZ versus MM ### p < 0.001, MZ versus MM $$$ p < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.

    Article Snippet: Mouse monoclonal anti-AAT polymers (clone 2C1) , Hycult Biotech , HM2289.

    Techniques: Flow Cytometry, Immunostaining, Inhibition, Labeling

    Journal: Cell reports

    Article Title: Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity

    doi: 10.1016/j.celrep.2022.111775

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-AAT polymers (clone 2C1) , Hycult Biotech , HM2289.

    Techniques: Recombinant, Electron Microscopy, Lysis, Lactate Assay, Pyruvate Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Sequencing, Mutagenesis, Software, Modification

    Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma Z-AAT polymers (µg/mL) were measured by ELISA based on LG96 mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.

    Journal: Biomolecules

    Article Title: The Relationship between Plasma Alpha-1-Antitrypsin Polymers and Lung or Liver Function in ZZ Alpha-1-Antitrypsin-Deficient Patients

    doi: 10.3390/biom12030380

    Figure Lengend Snippet: Pulmonary function of 52 ZZ-AATD patients was measured 15 min after the inhalation of 400 µg of salbutamol followed by spirometry and a CO diffusion gas transfer. Levels of plasma Z-AAT polymers (µg/mL) were measured by ELISA based on LG96 mAb (see materials and methods). There was no correlation between plasma Z-AAT polymer values and FEV1 % pred, or Kco % pred.

    Article Snippet: Membranes were blocked for 1 h with 5% low fat milk (Carl Roth, Karlsruhe, Germany) followed by overnight incubation at 4 °C with specific primary antibodies: mouse monoclonal anti-AAT polymer antibody (clone 2C1, 1:500, Hycult Biotech, Uden, The Netherlands), or mouse monoclonal anti-AAT polymer antibody (LG96, 1:500).

    Techniques: Diffusion-based Assay, Enzyme-linked Immunosorbent Assay

    Z-AAT polymer analysis by Western blots using two different monoclonal antibodies. Equal amounts of plasma samples from seven ZZ-AATD individuals were electrophoretically separated using 7.5% native polyacrylamide gels followed by western blots. Purified polymeric Z-AAT isolated from pooled ZZ plasma samples was used as a positive control. The western blots were probed against mouse monoclonal anti-AAT polymer antibodies LG96 ( A ) and 2C1 ( B )(both diluted 1:700). The image demonstrates that both antibodies recognize the Z-AAT polymers, but the profiles of recognized polymers differ. This blot is the representative from n = 2 independent repeats.

    Journal: Biomolecules

    Article Title: The Relationship between Plasma Alpha-1-Antitrypsin Polymers and Lung or Liver Function in ZZ Alpha-1-Antitrypsin-Deficient Patients

    doi: 10.3390/biom12030380

    Figure Lengend Snippet: Z-AAT polymer analysis by Western blots using two different monoclonal antibodies. Equal amounts of plasma samples from seven ZZ-AATD individuals were electrophoretically separated using 7.5% native polyacrylamide gels followed by western blots. Purified polymeric Z-AAT isolated from pooled ZZ plasma samples was used as a positive control. The western blots were probed against mouse monoclonal anti-AAT polymer antibodies LG96 ( A ) and 2C1 ( B )(both diluted 1:700). The image demonstrates that both antibodies recognize the Z-AAT polymers, but the profiles of recognized polymers differ. This blot is the representative from n = 2 independent repeats.

    Article Snippet: Membranes were blocked for 1 h with 5% low fat milk (Carl Roth, Karlsruhe, Germany) followed by overnight incubation at 4 °C with specific primary antibodies: mouse monoclonal anti-AAT polymer antibody (clone 2C1, 1:500, Hycult Biotech, Uden, The Netherlands), or mouse monoclonal anti-AAT polymer antibody (LG96, 1:500).

    Techniques: Western Blot, Purification, Isolation, Positive Control