Anti DNA Polymerase delta catalytic subunit antibody 607
https://www.bioz.com/result/mouse monoclonal anti pold1/product/Abcam
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1) Product Images from "Nucleotide selectivity defect and mutator phenotype conferred by a colon cancer-associated DNA polymerase δ mutation in human cells"
Article Title: Nucleotide selectivity defect and mutator phenotype conferred by a colon cancer-associated DNA polymerase δ mutation in human cells
Figure Legend Snippet: Expression of POLD1-R689W alters the specificity of spontaneous mutagenesis in HCT116 cells making it resemble the mutational specificity of HCT15. The diagrams show the HPRT1 mutation spectra of HCT116, HCT116 POLD1-R689W and HCT15 cell lines. The number of independent HPRT1 mutants analyzed (n) is indicated for each spectrum. Data for HCT116 POLD1-R689W and HCT116 are from Table 2 , and data for HCT15 is from reference 25 . Asterisks indicate statistically significant differences in the proportion of indels and GC→TA transversions between HCT116 and HCT116 POLD1-R689W spectra (p=0.000005 for indels and p=0.0043 for GC→TA transversions, Fisher’s exact text). No significant differences were observed between HCT116 POLD1-R689W and HCT15 spectra.
Techniques Used: Expressing, Mutagenesis
Figure Legend Snippet: Immunoblots showing p125 (POLD1) levels in clonal cell lines overexpressing POLD1 or POLD1-R689W . The numbers in parenthesis indicate independently derived cell lines. The left and right panels are from separate SDS-PAGE gels.
Techniques Used: Western Blot, Derivative Assay, SDS Page
Figure Legend Snippet: DNA sequence context of GC→TA transversions in cancer-related genes in the HCT15 cell line matches the mutational specificity of the POLD1-R689W mutator. (A) Expression of POLD1-R689W results in GC→TA transversions at polypurine/polypyrimidine tracts. Genomic sequence context is shown for all GC→TA transversions observed at the HPRT1 gene of the HCT116 POLD1-R689W cells. Each mutated site is shown as a separate entry with the number of mutations at this site in parentheses. The mutated base is underlined. (B) Sequence context of GC→TA transversions found in a set of 26 cancer-related genes in the HCT15 cell line. The set of genes was the same as the one used previously to characterize the mutational specificity of the NCI-60 panel of cell lines 19 and included APC , ARID1A , BRAF , DNMT1 , DNMT3A , DNMT3B , EGFR , EPHA3 , EPHA5 , EPHA7 , FBXW7 , GRIN2A , KRAS , LRP1B , NF2 , NRAS , PBRM1 , PIK3CA , POLE , PTEN , SETD2 , SPTA1 , STAG2 , SYNE1 , TP53 , and TRRAP . Sequence of the G-containing strand is shown. The mutated base is underlined.
Techniques Used: Sequencing, Expressing
Article Title: The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins
Article Snippet: .. Antibodies Used for Western Blotting The following antibodies were used for western blotting: β-actin-horseradish peroxidase (HRP) (C4, sc-47778; Santa Cruz Biotechnology), CIAO1 (ab83088; Abcam), Flag-M2, clone M2 (F1804; Sigma-Aldrich), MIP18/FAM96B (20108-1-AP; Proteintech), MMS19 (16015-1-AP; Proteintech), c-myc (A00704-100; Genescript),
Article Title: Distinct mechanistic responses to replication fork stalling induced by either nucleotide or protein deprivation
Article Snippet: The following antibodies were used in this study:
Article Title: Break-induced telomere synthesis underlies alternative telomere maintenance
Article Snippet: The following antibodies were used: anti-BrdU (mouse B44, BD 347580; rat BU1/75, AbD Serotec OBT0030G), anti-ATRX (rabbit H-300, Santa Cruz sc-15408), anti-53BP1 (rabbit, Novus NB100-904), anti-γH2AX (mouse JBW301, Millipore 05-636), anti-Flag (mouse M2, Sigma F1804), anti-PML (mouse PG-M3, Santa Cruz sc-966), anti-Rad51 (rabbit H-92, Santa Cruz sc-8349; mouse 14B4, Abcam ab-213), anti-Hop2/PSMC3IP (rabbit, Novus NBP1-92301), anti-POLD3 (mouse 3E2, Abnova H00010714-M01),
Article Title: A reduction of licensed origins reveals strain-specific replication dynamics in mice
Article Snippet: .. We used Abcam anti-MCM antibodies (ab3159, ab4460, ab4459, ab17967, ab4458 and ab2360 for MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7, respectively), anti-CDC45 (Abcam; ab56476),