mouse mesenchymal stem cell line c3h10t1 2  (ATCC)


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    Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs
    Description:
    Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs is a sterile phenol red free liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions system This low serum 2 FBS system is designed to support mesenchymal stem cells derived from various normal human tissues including lipoaspirates and umbilical cord Mesenchymal Stem Cell Basal Medium contains essential and non essential amino acids vitamins other organic compounds trace minerals and inorganic salts To support the proliferation and plating efficiency of various types of adult stem cells Mesenchymal Stem Cell Basal Medium must be supplemented with the appropriate cell specific growth kit When using this complete media system the growth of undifferentiated mesenchymal stem cells is supported without the use of feeder layers extracellular matrix proteins or other substrates For mesenchymal stem cells derived from human lipoaspirates e g Adipose Derived Mesenchymal Stem Cells Normal Human ATCC PCS 500 011 or umbilical cord Umbilical Cord Derived Mesenchymal Stem Cells Normal Human ATCC PCS 500 010 supplement Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs with the Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical derived MSCs Low serum ATCC PCS 500 040 For mesenchymal stem cells derived from bone marrow Bone Marrow derived Mesenchymal Stem Cells Normal Human ATCC PCS 500 012 supplement Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs with the Mesenchymal Stem Cell Growth Kit for Bone Marrow derived MSCs ATCC PCS 500 041 Optional media supplements Gentamicin Amphotericin B Solution ATCC PCS 999 025 Penicillin Streptomycin Amphotericin B Solution ATCC PCS 999 002 Phenol Red ATCC PCS 999 001
    Catalog Number:
    PCS-500-030
    Price:
    None
    Applications:
    Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This low serum (2% FBS) system is designed to support mesenchymal stem cells derived from various normal human tissues including lipoaspirates and umbilical cord. Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of adult stem cells, Mesenchymal Stem Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of undifferentiated mesenchymal stem cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.  For mesenchymal stem cells derived from human lipoaspirates (e.g., Adipose-Derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-011) or umbilical cord (Umbilical Cord-Derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-010), supplement Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs with the Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs –Low serum (ATCC PCS-500-040). For mesenchymal stem cells derived from bone marrow (Bone Marrow-derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-012) supplement Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs with the Mesenchymal  Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC PCS-500-041) Optional media supplements: Gentamicin-Amphotericin B Solution (ATCC PCS-999-025) Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002) Phenol Red (ATCC PCS-999-001)
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    Structured Review

    ATCC mouse mesenchymal stem cell line c3h10t1 2
    GPR54 is necessary for KP-10-induced osteoblast differentiation. ( A ) Wild-type and GPR54 −/− <t>C3H10T1/2</t> cells were treated with 50 μM KP-10 for 6, 9, 12 or 24 h. Western blot analysis was performed using the indicated antibodies. BMP2 expression was normalized to β-actin expression. And the densitometry analysis was performed by ImageJ program (A, lower panel). ( B and C ) RT-PCR was performed using total RNA isolated from cultured cells. Wild-type and GPR54 −/− cells were treated with 50 μM KP-10 for 1 day ( B and C ). C.M of wild-type cells treated with 50 μM KP-10 for 12 h was collected. GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 or C.M for 2 days ( C ).
    Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs is a sterile phenol red free liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions system This low serum 2 FBS system is designed to support mesenchymal stem cells derived from various normal human tissues including lipoaspirates and umbilical cord Mesenchymal Stem Cell Basal Medium contains essential and non essential amino acids vitamins other organic compounds trace minerals and inorganic salts To support the proliferation and plating efficiency of various types of adult stem cells Mesenchymal Stem Cell Basal Medium must be supplemented with the appropriate cell specific growth kit When using this complete media system the growth of undifferentiated mesenchymal stem cells is supported without the use of feeder layers extracellular matrix proteins or other substrates For mesenchymal stem cells derived from human lipoaspirates e g Adipose Derived Mesenchymal Stem Cells Normal Human ATCC PCS 500 011 or umbilical cord Umbilical Cord Derived Mesenchymal Stem Cells Normal Human ATCC PCS 500 010 supplement Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs with the Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical derived MSCs Low serum ATCC PCS 500 040 For mesenchymal stem cells derived from bone marrow Bone Marrow derived Mesenchymal Stem Cells Normal Human ATCC PCS 500 012 supplement Mesenchymal Stem Cell Basal Medium for Adipose Umbilical and Bone Marrow derived MSCs with the Mesenchymal Stem Cell Growth Kit for Bone Marrow derived MSCs ATCC PCS 500 041 Optional media supplements Gentamicin Amphotericin B Solution ATCC PCS 999 025 Penicillin Streptomycin Amphotericin B Solution ATCC PCS 999 002 Phenol Red ATCC PCS 999 001
    https://www.bioz.com/result/mouse mesenchymal stem cell line c3h10t1 2/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse mesenchymal stem cell line c3h10t1 2 - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Kisspeptin-10 (KP-10) stimulates osteoblast differentiation through GPR54-mediated regulation of BMP2 expression and activation"

    Article Title: Kisspeptin-10 (KP-10) stimulates osteoblast differentiation through GPR54-mediated regulation of BMP2 expression and activation

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-20571-2

    GPR54 is necessary for KP-10-induced osteoblast differentiation. ( A ) Wild-type and GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 for 6, 9, 12 or 24 h. Western blot analysis was performed using the indicated antibodies. BMP2 expression was normalized to β-actin expression. And the densitometry analysis was performed by ImageJ program (A, lower panel). ( B and C ) RT-PCR was performed using total RNA isolated from cultured cells. Wild-type and GPR54 −/− cells were treated with 50 μM KP-10 for 1 day ( B and C ). C.M of wild-type cells treated with 50 μM KP-10 for 12 h was collected. GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 or C.M for 2 days ( C ).
    Figure Legend Snippet: GPR54 is necessary for KP-10-induced osteoblast differentiation. ( A ) Wild-type and GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 for 6, 9, 12 or 24 h. Western blot analysis was performed using the indicated antibodies. BMP2 expression was normalized to β-actin expression. And the densitometry analysis was performed by ImageJ program (A, lower panel). ( B and C ) RT-PCR was performed using total RNA isolated from cultured cells. Wild-type and GPR54 −/− cells were treated with 50 μM KP-10 for 1 day ( B and C ). C.M of wild-type cells treated with 50 μM KP-10 for 12 h was collected. GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 or C.M for 2 days ( C ).

    Techniques Used: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Cell Culture

    KP-10 induces osteogenic gene expressions in C3H10T1/2 cells. ( A and B ) RT-PCR were performed using total RNA isolated from C3H10T1/2 cells treated with 5 or 50 μM KP-10 for 2 days ( A ), 50 μM KP-10 for 1 or 2 days ( B ). ( C ) Real-time PCR were performed using total RNA isolated cells treated with 50 μM of KP-10 for 2 days. * p
    Figure Legend Snippet: KP-10 induces osteogenic gene expressions in C3H10T1/2 cells. ( A and B ) RT-PCR were performed using total RNA isolated from C3H10T1/2 cells treated with 5 or 50 μM KP-10 for 2 days ( A ), 50 μM KP-10 for 1 or 2 days ( B ). ( C ) Real-time PCR were performed using total RNA isolated cells treated with 50 μM of KP-10 for 2 days. * p

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Isolation, Real-time Polymerase Chain Reaction

    2) Product Images from "Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK"

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    Journal: Molecules and Cells

    doi: 10.14348/molcells.2019.0136

    BMP2 treatment induces FTO expression in C3H10T1/2 cells (A and B) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with BMP2 (+, 0.125 μg/ml; ++, 0.25 μg/ml) for 1 day. (C and D) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with 0.25 μg/ml BMP2 for 12 or 24 h. (E and F) C3H10T1/2 cells were treated with BMP2 for the indicated durations and harvested for western blot analysis using the indicated antibodies. * P
    Figure Legend Snippet: BMP2 treatment induces FTO expression in C3H10T1/2 cells (A and B) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with BMP2 (+, 0.125 μg/ml; ++, 0.25 μg/ml) for 1 day. (C and D) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with 0.25 μg/ml BMP2 for 12 or 24 h. (E and F) C3H10T1/2 cells were treated with BMP2 for the indicated durations and harvested for western blot analysis using the indicated antibodies. * P

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Isolation, Western Blot

    Knock-down of FTO attenuates BMP2-induced osteogenic differentiation of C3H10T1/2 cells C3H10T1/2 cells were transfected with siFTO (+, 100 nM; ++, 200 nM) for 6 h and then treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells. (B) The real-time PCR analysis was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 4 days. ALP staining was performed. All experiments were independently repeated at least three times. Data represent the mean ± SEM of three individual experiments. * P
    Figure Legend Snippet: Knock-down of FTO attenuates BMP2-induced osteogenic differentiation of C3H10T1/2 cells C3H10T1/2 cells were transfected with siFTO (+, 100 nM; ++, 200 nM) for 6 h and then treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells. (B) The real-time PCR analysis was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 4 days. ALP staining was performed. All experiments were independently repeated at least three times. Data represent the mean ± SEM of three individual experiments. * P

    Techniques Used: Transfection, Reverse Transcription Polymerase Chain Reaction, Isolation, Real-time Polymerase Chain Reaction, Western Blot, Staining

    Overexpression of FTO induces osteogenic differentiation of C3H10T1/2 cells (A–C) C3H10T1/2 cells were transfected with pcDNA3.1 (2 μg) or pCMV-FTO (+, 1 μg; ++, 2 μg) for 6 h and treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells and primers targeting FTO, Dlx5, Runx2, and β-actin. (B) Real-time PCR was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with pCMV-FTO (+, 0.2 μg; ++, 0.4 μg) or treated with BMP2 (0.25 μg/ml) for 4 days. * P
    Figure Legend Snippet: Overexpression of FTO induces osteogenic differentiation of C3H10T1/2 cells (A–C) C3H10T1/2 cells were transfected with pcDNA3.1 (2 μg) or pCMV-FTO (+, 1 μg; ++, 2 μg) for 6 h and treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells and primers targeting FTO, Dlx5, Runx2, and β-actin. (B) Real-time PCR was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with pCMV-FTO (+, 0.2 μg; ++, 0.4 μg) or treated with BMP2 (0.25 μg/ml) for 4 days. * P

    Techniques Used: Over Expression, Transfection, Reverse Transcription Polymerase Chain Reaction, Isolation, Real-time Polymerase Chain Reaction, Western Blot

    Severe ER stress induced by TM abrogates BMP2-induced upregulation of FTO and p-AMPK (A) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 24 h. RT-PCR was performed using total RNA isolated from cells. (B) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 12 h. Western blotting was performed with the indicated antibodies. (C) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 4 days. ALP staining was performed. ** P
    Figure Legend Snippet: Severe ER stress induced by TM abrogates BMP2-induced upregulation of FTO and p-AMPK (A) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 24 h. RT-PCR was performed using total RNA isolated from cells. (B) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 12 h. Western blotting was performed with the indicated antibodies. (C) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 4 days. ALP staining was performed. ** P

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Isolation, Western Blot, Staining

    FTO and p-AMPK induce mild ER stress (A) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) and Com.C (1 μM) for 1 day. RT-PCR was performed using total RNA isolated from cells. (B and D) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then treated with Com.C (1 μM) for 24 h. (C and E) C3H10T1/2 cells were transfected with pc-DNA3.0-AMPK (2 μg) and siFTO for 6 h and then incubated for a further 12 h. RT-PCR (B and C) and western blot (D and E) analyses of ER stress markers (ATF4 and CHOP) were performed. All experiments were independently repeated at least three times.
    Figure Legend Snippet: FTO and p-AMPK induce mild ER stress (A) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) and Com.C (1 μM) for 1 day. RT-PCR was performed using total RNA isolated from cells. (B and D) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then treated with Com.C (1 μM) for 24 h. (C and E) C3H10T1/2 cells were transfected with pc-DNA3.0-AMPK (2 μg) and siFTO for 6 h and then incubated for a further 12 h. RT-PCR (B and C) and western blot (D and E) analyses of ER stress markers (ATF4 and CHOP) were performed. All experiments were independently repeated at least three times.

    Techniques Used: Transfection, Reverse Transcription Polymerase Chain Reaction, Isolation, Incubation, Western Blot

    FTO induces phosphorylation of AMPK in C3H10T1/2 cells (A) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then incubated for a further 12 h. Western blot assay analysis was performed using the indicated antibodies. (B) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 12 h. Western blot analysis was performed using the indicated antibodies. All experiments were independently repeated at least three times.
    Figure Legend Snippet: FTO induces phosphorylation of AMPK in C3H10T1/2 cells (A) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then incubated for a further 12 h. Western blot assay analysis was performed using the indicated antibodies. (B) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 12 h. Western blot analysis was performed using the indicated antibodies. All experiments were independently repeated at least three times.

    Techniques Used: Transfection, Incubation, Western Blot

    Activation of AMPK enhances FTO expression in C3H10T1/2 cells (A and B) C3H10T1/2 cells were transfected with pcDNA3.0-AMPK (2 μg) for 6 h and then incubated for a further 12 h. (A) RT-PCR analysis was performed using total RNA extracted from cells. (B) Western blot analysis was performed using the indicated antibodies. (C and D) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) for 24 h. Com.C (+, 0.1 μM; ++, 1 μM) was added 3 h prior to harvest. (C) RT-PCR analysis was performed using total RNA extracted from cells. (D) Western blot analysis was performed using the indicated antibodies. (E) C3H10T1/2 cells were transiently transfected with pc-DNA3.0AMPK (0.4 μg/well) and/or treated with BMP2 (0.25 μg/ml) for 4 days, and then stained with BCIP/NBT liquid substrate. ** P
    Figure Legend Snippet: Activation of AMPK enhances FTO expression in C3H10T1/2 cells (A and B) C3H10T1/2 cells were transfected with pcDNA3.0-AMPK (2 μg) for 6 h and then incubated for a further 12 h. (A) RT-PCR analysis was performed using total RNA extracted from cells. (B) Western blot analysis was performed using the indicated antibodies. (C and D) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) for 24 h. Com.C (+, 0.1 μM; ++, 1 μM) was added 3 h prior to harvest. (C) RT-PCR analysis was performed using total RNA extracted from cells. (D) Western blot analysis was performed using the indicated antibodies. (E) C3H10T1/2 cells were transiently transfected with pc-DNA3.0AMPK (0.4 μg/well) and/or treated with BMP2 (0.25 μg/ml) for 4 days, and then stained with BCIP/NBT liquid substrate. ** P

    Techniques Used: Activation Assay, Expressing, Transfection, Incubation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining

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    Article Snippet: .. Metabolic activity, viability, and morphology assays Metabolic activity of MSCs was assessed by XTT (ATCC) following 24, 48, or 72 hour exposure to budesonide. ..

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    Migration:

    Article Title: Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow
    Article Snippet: .. Endothelial Tube-Like Formation Stimulated by MSC Conditioned Medium The human umbilical vein endothelial cell (HUVEC) lineage EA.hy926 (ATCC® CRL-2922™), used for endothelial tube formation and migration assay, were evaluated in regard to CD31 expression and functional properties, as shown in . .. Representative images of tubular structures formed by HUVEC incubated with mbMSC and bmMSC conditioned medium, for 48 and 72 h of culture, carried out in normoxia and 1% hypoxia, are shown in A–H.

    Expressing:

    Article Title: Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow
    Article Snippet: .. Endothelial Tube-Like Formation Stimulated by MSC Conditioned Medium The human umbilical vein endothelial cell (HUVEC) lineage EA.hy926 (ATCC® CRL-2922™), used for endothelial tube formation and migration assay, were evaluated in regard to CD31 expression and functional properties, as shown in . .. Representative images of tubular structures formed by HUVEC incubated with mbMSC and bmMSC conditioned medium, for 48 and 72 h of culture, carried out in normoxia and 1% hypoxia, are shown in A–H.

    Functional Assay:

    Article Title: Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow
    Article Snippet: .. Endothelial Tube-Like Formation Stimulated by MSC Conditioned Medium The human umbilical vein endothelial cell (HUVEC) lineage EA.hy926 (ATCC® CRL-2922™), used for endothelial tube formation and migration assay, were evaluated in regard to CD31 expression and functional properties, as shown in . .. Representative images of tubular structures formed by HUVEC incubated with mbMSC and bmMSC conditioned medium, for 48 and 72 h of culture, carried out in normoxia and 1% hypoxia, are shown in A–H.

    In Vitro:

    Article Title: A Regulatory miRNA–mRNA Network Is Associated with Tissue Repair Induced by Mesenchymal Stromal Cells in Acute Kidney Injury
    Article Snippet: A FACSCanto II flow cytometer (BD, Beckton Dickson) was used for cell acquisition, and the FlowJo software was used for data analysis. .. Coculture of MSCs or MVs with Renal Tubular Cells For in vitro assays, approximately 2 × 105 of renal epithelial tubular cells (MM55.K, ATCC® CRL-6436TM) were seeded in six-well plates (TPP, USA) and treated with nephrotoxic drug cisplatin (8 µg/mL) for 48 h, and two additional treated groups with cisplatin were co-cultured in contact with MSCs (v/v 1:1, 1 × 105 ) or MVs (50 µg/ml, sequentially each 6 h) for 48 h (Figure S4C in Supplementary Material). .. Subsequently, cells were trypsinized and subjected to analysis for apoptosis, cell proliferation, and oxidative stress analysis using the respective kits: Alexa Fluor® 488 annexin V/Dead Cell Apoptosis kit, CellTrace™ Violet Cell Proliferation kit, and MitoSOX™ Red Mitochondrial Superoxide Indicator kit (Life Technologies, USA), following the manufacturer’s recommendations (n = 6).

    Cell Culture:

    Article Title: Mesenchymal Stem/Stromal Cells in Stromal Evolution and Cancer Progression
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    Derivative Assay:

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  • 99
    ATCC mouse mesenchymal stem cell line c3h10t1 2
    GPR54 is necessary for KP-10-induced osteoblast differentiation. ( A ) Wild-type and GPR54 −/− <t>C3H10T1/2</t> cells were treated with 50 μM KP-10 for 6, 9, 12 or 24 h. Western blot analysis was performed using the indicated antibodies. BMP2 expression was normalized to β-actin expression. And the densitometry analysis was performed by ImageJ program (A, lower panel). ( B and C ) RT-PCR was performed using total RNA isolated from cultured cells. Wild-type and GPR54 −/− cells were treated with 50 μM KP-10 for 1 day ( B and C ). C.M of wild-type cells treated with 50 μM KP-10 for 12 h was collected. GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 or C.M for 2 days ( C ).
    Mouse Mesenchymal Stem Cell Line C3h10t1 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse mesenchymal stem cell line c3h10t1 2/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse mesenchymal stem cell line c3h10t1 2 - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    97
    ATCC murine mesenchymal stem cell line c3h10t1 2 the c3h10t1 2
    The effects of Sirt1 pharmacologic activation and inhibition on adipogenic markers in C3HT101/2 cells (A) . Oil-red-o staining in <t>C3H10T1/2</t> cells induced to adipogenesis and supplemented with SRT3025 or vehicle (DMSO). (B,C) Gene expression analysis of adipocyte (B) and mitochondrial markers (C) induced to adipogenesis and supplemented with SRT3025 or vehicle (DMSO). (D) Gene expression analysis of adipocyte markers induced to adipogenesis and supplemented with Ex527 or vehicle (DMSO). Results are Mean ± SEM. * P
    Murine Mesenchymal Stem Cell Line C3h10t1 2 The C3h10t1 2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GPR54 is necessary for KP-10-induced osteoblast differentiation. ( A ) Wild-type and GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 for 6, 9, 12 or 24 h. Western blot analysis was performed using the indicated antibodies. BMP2 expression was normalized to β-actin expression. And the densitometry analysis was performed by ImageJ program (A, lower panel). ( B and C ) RT-PCR was performed using total RNA isolated from cultured cells. Wild-type and GPR54 −/− cells were treated with 50 μM KP-10 for 1 day ( B and C ). C.M of wild-type cells treated with 50 μM KP-10 for 12 h was collected. GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 or C.M for 2 days ( C ).

    Journal: Scientific Reports

    Article Title: Kisspeptin-10 (KP-10) stimulates osteoblast differentiation through GPR54-mediated regulation of BMP2 expression and activation

    doi: 10.1038/s41598-018-20571-2

    Figure Lengend Snippet: GPR54 is necessary for KP-10-induced osteoblast differentiation. ( A ) Wild-type and GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 for 6, 9, 12 or 24 h. Western blot analysis was performed using the indicated antibodies. BMP2 expression was normalized to β-actin expression. And the densitometry analysis was performed by ImageJ program (A, lower panel). ( B and C ) RT-PCR was performed using total RNA isolated from cultured cells. Wild-type and GPR54 −/− cells were treated with 50 μM KP-10 for 1 day ( B and C ). C.M of wild-type cells treated with 50 μM KP-10 for 12 h was collected. GPR54 −/− C3H10T1/2 cells were treated with 50 μM KP-10 or C.M for 2 days ( C ).

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (ATCC, Manassas, VA) was maintained in DMEM containing 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin in humidified air containing 5% CO2 at 37 °C.

    Techniques: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Cell Culture

    KP-10 induces osteogenic gene expressions in C3H10T1/2 cells. ( A and B ) RT-PCR were performed using total RNA isolated from C3H10T1/2 cells treated with 5 or 50 μM KP-10 for 2 days ( A ), 50 μM KP-10 for 1 or 2 days ( B ). ( C ) Real-time PCR were performed using total RNA isolated cells treated with 50 μM of KP-10 for 2 days. * p

    Journal: Scientific Reports

    Article Title: Kisspeptin-10 (KP-10) stimulates osteoblast differentiation through GPR54-mediated regulation of BMP2 expression and activation

    doi: 10.1038/s41598-018-20571-2

    Figure Lengend Snippet: KP-10 induces osteogenic gene expressions in C3H10T1/2 cells. ( A and B ) RT-PCR were performed using total RNA isolated from C3H10T1/2 cells treated with 5 or 50 μM KP-10 for 2 days ( A ), 50 μM KP-10 for 1 or 2 days ( B ). ( C ) Real-time PCR were performed using total RNA isolated cells treated with 50 μM of KP-10 for 2 days. * p

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (ATCC, Manassas, VA) was maintained in DMEM containing 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin in humidified air containing 5% CO2 at 37 °C.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation, Real-time Polymerase Chain Reaction

    BMP2 treatment induces FTO expression in C3H10T1/2 cells (A and B) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with BMP2 (+, 0.125 μg/ml; ++, 0.25 μg/ml) for 1 day. (C and D) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with 0.25 μg/ml BMP2 for 12 or 24 h. (E and F) C3H10T1/2 cells were treated with BMP2 for the indicated durations and harvested for western blot analysis using the indicated antibodies. * P

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: BMP2 treatment induces FTO expression in C3H10T1/2 cells (A and B) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with BMP2 (+, 0.125 μg/ml; ++, 0.25 μg/ml) for 1 day. (C and D) RT-PCR and real-time PCR analyses were performed using total RNA isolated from C3H10T1/2 cells treated with 0.25 μg/ml BMP2 for 12 or 24 h. (E and F) C3H10T1/2 cells were treated with BMP2 for the indicated durations and harvested for western blot analysis using the indicated antibodies. * P

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Isolation, Western Blot

    Knock-down of FTO attenuates BMP2-induced osteogenic differentiation of C3H10T1/2 cells C3H10T1/2 cells were transfected with siFTO (+, 100 nM; ++, 200 nM) for 6 h and then treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells. (B) The real-time PCR analysis was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 4 days. ALP staining was performed. All experiments were independently repeated at least three times. Data represent the mean ± SEM of three individual experiments. * P

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: Knock-down of FTO attenuates BMP2-induced osteogenic differentiation of C3H10T1/2 cells C3H10T1/2 cells were transfected with siFTO (+, 100 nM; ++, 200 nM) for 6 h and then treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells. (B) The real-time PCR analysis was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 4 days. ALP staining was performed. All experiments were independently repeated at least three times. Data represent the mean ± SEM of three individual experiments. * P

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Isolation, Real-time Polymerase Chain Reaction, Western Blot, Staining

    Overexpression of FTO induces osteogenic differentiation of C3H10T1/2 cells (A–C) C3H10T1/2 cells were transfected with pcDNA3.1 (2 μg) or pCMV-FTO (+, 1 μg; ++, 2 μg) for 6 h and treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells and primers targeting FTO, Dlx5, Runx2, and β-actin. (B) Real-time PCR was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with pCMV-FTO (+, 0.2 μg; ++, 0.4 μg) or treated with BMP2 (0.25 μg/ml) for 4 days. * P

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: Overexpression of FTO induces osteogenic differentiation of C3H10T1/2 cells (A–C) C3H10T1/2 cells were transfected with pcDNA3.1 (2 μg) or pCMV-FTO (+, 1 μg; ++, 2 μg) for 6 h and treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells and primers targeting FTO, Dlx5, Runx2, and β-actin. (B) Real-time PCR was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with pCMV-FTO (+, 0.2 μg; ++, 0.4 μg) or treated with BMP2 (0.25 μg/ml) for 4 days. * P

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Over Expression, Transfection, Reverse Transcription Polymerase Chain Reaction, Isolation, Real-time Polymerase Chain Reaction, Western Blot

    Severe ER stress induced by TM abrogates BMP2-induced upregulation of FTO and p-AMPK (A) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 24 h. RT-PCR was performed using total RNA isolated from cells. (B) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 12 h. Western blotting was performed with the indicated antibodies. (C) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 4 days. ALP staining was performed. ** P

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: Severe ER stress induced by TM abrogates BMP2-induced upregulation of FTO and p-AMPK (A) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 24 h. RT-PCR was performed using total RNA isolated from cells. (B) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 12 h. Western blotting was performed with the indicated antibodies. (C) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) and/or TM (100 ng/ml) for 4 days. ALP staining was performed. ** P

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation, Western Blot, Staining

    FTO and p-AMPK induce mild ER stress (A) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) and Com.C (1 μM) for 1 day. RT-PCR was performed using total RNA isolated from cells. (B and D) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then treated with Com.C (1 μM) for 24 h. (C and E) C3H10T1/2 cells were transfected with pc-DNA3.0-AMPK (2 μg) and siFTO for 6 h and then incubated for a further 12 h. RT-PCR (B and C) and western blot (D and E) analyses of ER stress markers (ATF4 and CHOP) were performed. All experiments were independently repeated at least three times.

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: FTO and p-AMPK induce mild ER stress (A) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) and Com.C (1 μM) for 1 day. RT-PCR was performed using total RNA isolated from cells. (B and D) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then treated with Com.C (1 μM) for 24 h. (C and E) C3H10T1/2 cells were transfected with pc-DNA3.0-AMPK (2 μg) and siFTO for 6 h and then incubated for a further 12 h. RT-PCR (B and C) and western blot (D and E) analyses of ER stress markers (ATF4 and CHOP) were performed. All experiments were independently repeated at least three times.

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Isolation, Incubation, Western Blot

    FTO induces phosphorylation of AMPK in C3H10T1/2 cells (A) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then incubated for a further 12 h. Western blot assay analysis was performed using the indicated antibodies. (B) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 12 h. Western blot analysis was performed using the indicated antibodies. All experiments were independently repeated at least three times.

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: FTO induces phosphorylation of AMPK in C3H10T1/2 cells (A) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then incubated for a further 12 h. Western blot assay analysis was performed using the indicated antibodies. (B) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 12 h. Western blot analysis was performed using the indicated antibodies. All experiments were independently repeated at least three times.

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Transfection, Incubation, Western Blot

    Activation of AMPK enhances FTO expression in C3H10T1/2 cells (A and B) C3H10T1/2 cells were transfected with pcDNA3.0-AMPK (2 μg) for 6 h and then incubated for a further 12 h. (A) RT-PCR analysis was performed using total RNA extracted from cells. (B) Western blot analysis was performed using the indicated antibodies. (C and D) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) for 24 h. Com.C (+, 0.1 μM; ++, 1 μM) was added 3 h prior to harvest. (C) RT-PCR analysis was performed using total RNA extracted from cells. (D) Western blot analysis was performed using the indicated antibodies. (E) C3H10T1/2 cells were transiently transfected with pc-DNA3.0AMPK (0.4 μg/well) and/or treated with BMP2 (0.25 μg/ml) for 4 days, and then stained with BCIP/NBT liquid substrate. ** P

    Journal: Molecules and Cells

    Article Title: Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK

    doi: 10.14348/molcells.2019.0136

    Figure Lengend Snippet: Activation of AMPK enhances FTO expression in C3H10T1/2 cells (A and B) C3H10T1/2 cells were transfected with pcDNA3.0-AMPK (2 μg) for 6 h and then incubated for a further 12 h. (A) RT-PCR analysis was performed using total RNA extracted from cells. (B) Western blot analysis was performed using the indicated antibodies. (C and D) C3H10T1/2 cells were treated with BMP2 (0.25 μg/ml) for 24 h. Com.C (+, 0.1 μM; ++, 1 μM) was added 3 h prior to harvest. (C) RT-PCR analysis was performed using total RNA extracted from cells. (D) Western blot analysis was performed using the indicated antibodies. (E) C3H10T1/2 cells were transiently transfected with pc-DNA3.0AMPK (0.4 μg/well) and/or treated with BMP2 (0.25 μg/ml) for 4 days, and then stained with BCIP/NBT liquid substrate. ** P

    Article Snippet: Cell culture The mouse mesenchymal stem cell line C3H10T1/2 (American Type Culture Collection [ATCC], USA) was maintained in DMEM containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in humidified air containing 5% CO2 at 37°C.

    Techniques: Activation Assay, Expressing, Transfection, Incubation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining

    The effects of Sirt1 pharmacologic activation and inhibition on adipogenic markers in C3HT101/2 cells (A) . Oil-red-o staining in C3H10T1/2 cells induced to adipogenesis and supplemented with SRT3025 or vehicle (DMSO). (B,C) Gene expression analysis of adipocyte (B) and mitochondrial markers (C) induced to adipogenesis and supplemented with SRT3025 or vehicle (DMSO). (D) Gene expression analysis of adipocyte markers induced to adipogenesis and supplemented with Ex527 or vehicle (DMSO). Results are Mean ± SEM. * P

    Journal: Frontiers in Endocrinology

    Article Title: Sirt1 Promotes a Thermogenic Gene Program in Bone Marrow Adipocytes: From Mice to (Wo)Men

    doi: 10.3389/fendo.2019.00126

    Figure Lengend Snippet: The effects of Sirt1 pharmacologic activation and inhibition on adipogenic markers in C3HT101/2 cells (A) . Oil-red-o staining in C3H10T1/2 cells induced to adipogenesis and supplemented with SRT3025 or vehicle (DMSO). (B,C) Gene expression analysis of adipocyte (B) and mitochondrial markers (C) induced to adipogenesis and supplemented with SRT3025 or vehicle (DMSO). (D) Gene expression analysis of adipocyte markers induced to adipogenesis and supplemented with Ex527 or vehicle (DMSO). Results are Mean ± SEM. * P

    Article Snippet: Experiments in the Murine Mesenchymal Stem-Cell Line C3H10T1/2 The C3H10T1/2 (ATCC CCL-226) murine mesenchymal stem cell line is an established cell line model to investigate bone marrow adipocytes ( , ).

    Techniques: Activation Assay, Inhibition, Staining, Expressing

    The effect of Sirt1 over-expression on adipogenesis in C3HT101/2 cells (A) . Oil-red-o staining in Sirt1 over-expressing ( OE ) and C3H10T1/2 cells induced to adipogenesis. Data is presented as optical density (OD) corrected for cell number (crystal violet staining). Scale bar 500μm. (B,C) Immunoblot of Pgc1α and Prdm16 in Sirt1 OE and C3H10T1/2 cells 7 days post induction to adipogenesis. Results are Mean ± SEM. * P

    Journal: Frontiers in Endocrinology

    Article Title: Sirt1 Promotes a Thermogenic Gene Program in Bone Marrow Adipocytes: From Mice to (Wo)Men

    doi: 10.3389/fendo.2019.00126

    Figure Lengend Snippet: The effect of Sirt1 over-expression on adipogenesis in C3HT101/2 cells (A) . Oil-red-o staining in Sirt1 over-expressing ( OE ) and C3H10T1/2 cells induced to adipogenesis. Data is presented as optical density (OD) corrected for cell number (crystal violet staining). Scale bar 500μm. (B,C) Immunoblot of Pgc1α and Prdm16 in Sirt1 OE and C3H10T1/2 cells 7 days post induction to adipogenesis. Results are Mean ± SEM. * P

    Article Snippet: Experiments in the Murine Mesenchymal Stem-Cell Line C3H10T1/2 The C3H10T1/2 (ATCC CCL-226) murine mesenchymal stem cell line is an established cell line model to investigate bone marrow adipocytes ( , ).

    Techniques: Over Expression, Staining, Expressing

    BMP activity in C3H10T1/2 cells. (a) C3H10T1/2 cells infected with BRE-Luc construct were treated with increasing concentration of rhBMP2, rhBMP12, or rhBMP13. (b) ALP activity in C3H10T1/2 cells following 2-day incubation with 0, 1, 10, or 100nM of rhBMP2, rhBMP12, or rhBMP13.

    Journal: Growth Factors (Chur, Switzerland)

    Article Title: Divergent activities of osteogenic BMP2, and tenogenic BMP12 and BMP13 independent of receptor binding affinities

    doi: 10.3109/08977194.2011.593178

    Figure Lengend Snippet: BMP activity in C3H10T1/2 cells. (a) C3H10T1/2 cells infected with BRE-Luc construct were treated with increasing concentration of rhBMP2, rhBMP12, or rhBMP13. (b) ALP activity in C3H10T1/2 cells following 2-day incubation with 0, 1, 10, or 100nM of rhBMP2, rhBMP12, or rhBMP13.

    Article Snippet: Cell culture conditions The murine mesenchymal stem cell line C3H10T1/2 (clone 8) was purchased from ATCC (CCL-226; Manassas, VA, USA).

    Techniques: Activity Assay, Infection, Construct, Concentration Assay, ALP Assay, Incubation

    Induction of SMAD signaling by BMPs in C3H10T1/2 cells. Cell lysates from rhBMP2, rhBMP12, or rhBMP13 treated C3H10T1/2 cells were analyzed by western blot with antibodies to phosphorylated SMAD 1/5/8 and to β-actin or tubulin. Cells were treated with (a)10 nM BMPs for 0 to 30 min, (b) 10 nM BMPs for 0 to 120 min or (c) treated with 100 nM BMPs for 0 to 3 h.

    Journal: Growth Factors (Chur, Switzerland)

    Article Title: Divergent activities of osteogenic BMP2, and tenogenic BMP12 and BMP13 independent of receptor binding affinities

    doi: 10.3109/08977194.2011.593178

    Figure Lengend Snippet: Induction of SMAD signaling by BMPs in C3H10T1/2 cells. Cell lysates from rhBMP2, rhBMP12, or rhBMP13 treated C3H10T1/2 cells were analyzed by western blot with antibodies to phosphorylated SMAD 1/5/8 and to β-actin or tubulin. Cells were treated with (a)10 nM BMPs for 0 to 30 min, (b) 10 nM BMPs for 0 to 120 min or (c) treated with 100 nM BMPs for 0 to 3 h.

    Article Snippet: Cell culture conditions The murine mesenchymal stem cell line C3H10T1/2 (clone 8) was purchased from ATCC (CCL-226; Manassas, VA, USA).

    Techniques: Western Blot

    Expression of bone, tendon, and cartilage markers by BMPs in C3H10T1/2 cells. mRNA levels were determined by Taqman for the bone markers (a) Ocn (osteocalcin), (b) Runx2 (runt-related transcription factor 2, (c) Alpl (ALP), and (d) Sp7 (osterix), the tendon markers (e) Thbs4 (thrombospondin 4) and (f) Tnmd , (tenomodulin) and the cartilage markers (g) Sox9 (SRY-box containing gene 9), (h) Col2a1 (collagen, type II, alpha 1), (i) Acan (Aggrecan 1) and (j) Col11a1 (collagen, type 11, alpha 1).

    Journal: Growth Factors (Chur, Switzerland)

    Article Title: Divergent activities of osteogenic BMP2, and tenogenic BMP12 and BMP13 independent of receptor binding affinities

    doi: 10.3109/08977194.2011.593178

    Figure Lengend Snippet: Expression of bone, tendon, and cartilage markers by BMPs in C3H10T1/2 cells. mRNA levels were determined by Taqman for the bone markers (a) Ocn (osteocalcin), (b) Runx2 (runt-related transcription factor 2, (c) Alpl (ALP), and (d) Sp7 (osterix), the tendon markers (e) Thbs4 (thrombospondin 4) and (f) Tnmd , (tenomodulin) and the cartilage markers (g) Sox9 (SRY-box containing gene 9), (h) Col2a1 (collagen, type II, alpha 1), (i) Acan (Aggrecan 1) and (j) Col11a1 (collagen, type 11, alpha 1).

    Article Snippet: Cell culture conditions The murine mesenchymal stem cell line C3H10T1/2 (clone 8) was purchased from ATCC (CCL-226; Manassas, VA, USA).

    Techniques: Expressing, ALP Assay