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Santa Cruz Biotechnology mouse matrin 3 sirna
Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the <t>Matrin-3</t> protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .
Mouse Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells"

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

Journal: Scientific Reports

doi: 10.1038/s41598-018-31597-x

Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .
Figure Legend Snippet: Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .

Techniques Used: Mass Spectrometry, Expressing

In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).
Figure Legend Snippet: In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).

Techniques Used: In Vitro, In Vivo, Expressing, De-Phosphorylation Assay, Immunostaining, Staining

Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.
Figure Legend Snippet: Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.

Techniques Used: In Vitro, In Vivo, Immunostaining, In Utero, Expressing, Staining

ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.
Figure Legend Snippet: ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.

Techniques Used: Inhibition, In Vitro, Mutagenesis, Transfection, Translocation Assay

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    Santa Cruz Biotechnology mouse matrin 3 sirna
    Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the <t>Matrin-3</t> protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .
    Mouse Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse matrin 3 sirna/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse matrin 3 sirna - by Bioz Stars, 2024-04
    92/100 stars
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    Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: Mass Spectrometry, Expressing

    In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: In Vitro, In Vivo, Expressing, De-Phosphorylation Assay, Immunostaining, Staining

    Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: In Vitro, In Vivo, Immunostaining, In Utero, Expressing, Staining

    ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: Inhibition, In Vitro, Mutagenesis, Transfection, Translocation Assay