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ATCC mouse macrophage cell line raw264 7
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Mouse Macrophage Cell Line Raw264 7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC mouse macrophage like cell line j774
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
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Biotechnology Information mouse macrophage cell line
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
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Ubigene Biosciences Co Ltd mouse macrophage cell line
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
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Lonza j774 1 mouse macrophage cell line j774 1 macrophages
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
J774 1 Mouse Macrophage Cell Line J774 1 Macrophages, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC raw264 7 mouse macrophage cell line
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Raw264 7 Mouse Macrophage Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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China Center for Type Culture Collection mouse macrophage cell line
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Mouse Macrophage Cell Line, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iCell Bioscience Inc mouse leukemic monocyte macrophage raw264 7 cell lines
O-linked β-N-acetylglucosamine in <t>RAW264.7</t> Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Mouse Leukemic Monocyte Macrophage Raw264 7 Cell Lines, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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O-linked β-N-acetylglucosamine in RAW264.7 Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Journal: World Journal of Diabetes

Article Title: O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: In vivo and in vitro study

doi: 10.4239/wjd.v16.i3.95092

Figure Lengend Snippet: O-linked β-N-acetylglucosamine in RAW264.7 Cells Treated with high glucose and lipopolysaccharide. A: Total O-linked β-N-acetylglucosamine levels; B: Protein levels of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were measured by Western blot; C: Protein levels of OGT and OGA were quantified. a P < 0.001. P value calculated vs control. LPS: Lipopolysaccharide; HG: High glucose; OGT: O-GlcNAc transferase; OGA: O-GlcNAcase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Mouse macrophage cell line RAW264.7 was obtained from American type culture collection (Manassas, VA, United States).

Techniques: Western Blot, Control

O-GlcNAc transferase promotes O-linked β-N-acetylglucosamine and phosphorylation of p38. A: The interaction between O-GlcNAc transferase (OGT) and p38 was analyzed using co-immunoprecipitation; B: The localization of OGT and p38 in RAW264.7 cells was observed using immunofluorescence staining. Scale bar: 20 μm; C: Potential O-linked β-N-acetylglucosamine (O-GlcNAcylation) sites in p38 were predicted using the DictyOGlyc 1.1 Server software; D: The O-GlcNAcylation sites were confirmed, and the effects on p38 phosphorylation were measured using Western blot; E: The relative protein expression levels were quantified. a P < 0.05. P value calculated vs wild-type. OGT: O-GlcNAc transferase; DAPI: 4’,6-Diamidino-2-phenylindole; WT: Wild-type; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; RL2: O-GlcNAc.

Journal: World Journal of Diabetes

Article Title: O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: In vivo and in vitro study

doi: 10.4239/wjd.v16.i3.95092

Figure Lengend Snippet: O-GlcNAc transferase promotes O-linked β-N-acetylglucosamine and phosphorylation of p38. A: The interaction between O-GlcNAc transferase (OGT) and p38 was analyzed using co-immunoprecipitation; B: The localization of OGT and p38 in RAW264.7 cells was observed using immunofluorescence staining. Scale bar: 20 μm; C: Potential O-linked β-N-acetylglucosamine (O-GlcNAcylation) sites in p38 were predicted using the DictyOGlyc 1.1 Server software; D: The O-GlcNAcylation sites were confirmed, and the effects on p38 phosphorylation were measured using Western blot; E: The relative protein expression levels were quantified. a P < 0.05. P value calculated vs wild-type. OGT: O-GlcNAc transferase; DAPI: 4’,6-Diamidino-2-phenylindole; WT: Wild-type; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; RL2: O-GlcNAc.

Article Snippet: Mouse macrophage cell line RAW264.7 was obtained from American type culture collection (Manassas, VA, United States).

Techniques: Immunoprecipitation, Immunofluorescence, Staining, Software, Western Blot, Expressing