mouse mab10e2 hdac1 5356s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse mab10e2 hdac1 5356s
    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and <t>HDAC1</t> (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
    Mouse Mab10e2 Hdac1 5356s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse mab10e2 hdac1 5356s/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse mab10e2 hdac1 5356s - by Bioz Stars, 2023-03
    86/100 stars

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    1) Product Images from "EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells"

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

    Journal: bioRxiv

    doi: 10.1101/619080

    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
    Figure Legend Snippet: (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.

    Techniques Used: Western Blot, Methylation, Stable Transfection, Expressing

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    • mouse mab10e2 hdac1 5356s  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc mouse mab10e2 hdac1 5356s
    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and <t>HDAC1</t> (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
    Mouse Mab10e2 Hdac1 5356s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse mab10e2 hdac1 5356s/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse mab10e2 hdac1 5356s - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.

    Journal: bioRxiv

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

    doi: 10.1101/619080

    Figure Lengend Snippet: (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.

    Article Snippet: Polyclonal Rabbit one against H3 from Cell Signaling Technology (9715 ) ; H3K9me2 (ab1220) and H3K27Ac (ab 4729) were purchased from Abcam; H3K27me1 mouse mAb C0321 from Active Motif; H3K27me3 Rabbit mAb C36B11 (9733), H3K4me3 Rabbit mAb C42D8 (9751), Rabbit mAb D7C6X (14129), Rabbit mAb H2AK119ub (8240S) and mouse mAb10E2 HDAC1 (5356S) from Ozyme (Cell Signaling Technology). hEZHIP (HPA006128) and mAb Flag-M2 (F1804) purchased from SIGMA; Anti-Germ cell-specific Rabbit Polyclonal DPP3A/Stella (19878) and TRA98 (Ab82527) Rat monoclonal one from Abcam.

    Techniques: Western Blot, Methylation, Stable Transfection, Expressing