mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
    94/100 stars

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    mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
    94/100 stars

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    mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    E. cloacae B29 induction of systemic and local inflammation in HFD-fed NAFLD GF mice is dependent upon endotoxin expression. GF mice were fed either normal chow diet (NCD) or high-fat diet (HFD) and inoculated with either Luria-Bertani broth (LB), wild-type E. cloacae B29, or the endotoxin-lacking waaG mutant strain (data were collected at the end of 15 weeks after inoculation). (A) <t>ELISA</t> of serum LPS-binding <t>protein</t> <t>(LBP).</t> (B) ELISA of serum amyloid A (SAA-3). (C) RT-qPCR analysis of expression of the Tlr4 gene in the liver. (D to F) RT-qPCR analysis of expression of the Tnfα , Il1β , Mcp1 , Ikkε , and Reg3γ genes in the liver (D), ileum (E), and epididymal fat pad (F). All mRNA quantification data were normalized against the housekeeping gene. Gene expression levels were expressed as values relative to those of the control group (NCD+LB). Tlr4 , toll-like receptor 4 gene; Tnfα , tumor necrosis factor-α gene; Il1β , interleukin-1β gene; Mcp1 , monocyte chemoattractant protein-1 gene; Ikkε , I kappa B kinase epsilon gene; Reg3γ , c-type lectin regenerating islet-derived protein 3-γ. Data shown are means ± SEM ( n = 5 to 10). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
    94/100 stars

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    1) Product Images from "Endotoxin Producers Overgrowing in Human Gut Microbiota as the Causative Agents for Nonalcoholic Fatty Liver Disease"

    Article Title: Endotoxin Producers Overgrowing in Human Gut Microbiota as the Causative Agents for Nonalcoholic Fatty Liver Disease

    Journal: mBio

    doi: 10.1128/mBio.03263-19

    E. cloacae B29 induction of systemic and local inflammation in HFD-fed NAFLD GF mice is dependent upon endotoxin expression. GF mice were fed either normal chow diet (NCD) or high-fat diet (HFD) and inoculated with either Luria-Bertani broth (LB), wild-type E. cloacae B29, or the endotoxin-lacking waaG mutant strain (data were collected at the end of 15 weeks after inoculation). (A) ELISA of serum LPS-binding protein (LBP). (B) ELISA of serum amyloid A (SAA-3). (C) RT-qPCR analysis of expression of the Tlr4 gene in the liver. (D to F) RT-qPCR analysis of expression of the Tnfα , Il1β , Mcp1 , Ikkε , and Reg3γ genes in the liver (D), ileum (E), and epididymal fat pad (F). All mRNA quantification data were normalized against the housekeeping gene. Gene expression levels were expressed as values relative to those of the control group (NCD+LB). Tlr4 , toll-like receptor 4 gene; Tnfα , tumor necrosis factor-α gene; Il1β , interleukin-1β gene; Mcp1 , monocyte chemoattractant protein-1 gene; Ikkε , I kappa B kinase epsilon gene; Reg3γ , c-type lectin regenerating islet-derived protein 3-γ. Data shown are means ± SEM ( n = 5 to 10). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.
    Figure Legend Snippet: E. cloacae B29 induction of systemic and local inflammation in HFD-fed NAFLD GF mice is dependent upon endotoxin expression. GF mice were fed either normal chow diet (NCD) or high-fat diet (HFD) and inoculated with either Luria-Bertani broth (LB), wild-type E. cloacae B29, or the endotoxin-lacking waaG mutant strain (data were collected at the end of 15 weeks after inoculation). (A) ELISA of serum LPS-binding protein (LBP). (B) ELISA of serum amyloid A (SAA-3). (C) RT-qPCR analysis of expression of the Tlr4 gene in the liver. (D to F) RT-qPCR analysis of expression of the Tnfα , Il1β , Mcp1 , Ikkε , and Reg3γ genes in the liver (D), ileum (E), and epididymal fat pad (F). All mRNA quantification data were normalized against the housekeeping gene. Gene expression levels were expressed as values relative to those of the control group (NCD+LB). Tlr4 , toll-like receptor 4 gene; Tnfα , tumor necrosis factor-α gene; Il1β , interleukin-1β gene; Mcp1 , monocyte chemoattractant protein-1 gene; Ikkε , I kappa B kinase epsilon gene; Reg3γ , c-type lectin regenerating islet-derived protein 3-γ. Data shown are means ± SEM ( n = 5 to 10). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.

    Techniques Used: Expressing, Mutagenesis, Enzyme-linked Immunosorbent Assay, Binding Assay, Quantitative RT-PCR, Derivative Assay

    Absence of TLR4 in mice prevented NAFLD induced by the LPS-producing gut opportunistic pathobiont Enterobacter cloacae B29 (data were collected at the end of 15 weeks after inoculation). (A) Liver histology (hematoxylin and eosin stain) of TLR4 mutant (TLR4 −/− ) mice with or without B29 at the end of the trial. Scale bar, 100 μm. (B) NAFLD activity score. (C) Body weight. (D) Mass of epididymal, mesenteric, subcutaneous inguinal, and retroperitoneal fat pads of TLR4 −/− mice with or without B29. (E) Plasma insulin concentration before (fasting) and 30 min after oral glucose load in TLR4 −/− mice with or without B29. (F) ELISA of serum leptin in TLR4 −/− mice with or without B29 (after adjustment for body weight). (G) ELISA of serum LBP in TLR4 −/− mice with or without B29. (H) ELISA of serum amyloid A (SAA-3) in TLR4 −/− mice with or without B29. Data shown are means ± SEM ( n = 5 to 9). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.
    Figure Legend Snippet: Absence of TLR4 in mice prevented NAFLD induced by the LPS-producing gut opportunistic pathobiont Enterobacter cloacae B29 (data were collected at the end of 15 weeks after inoculation). (A) Liver histology (hematoxylin and eosin stain) of TLR4 mutant (TLR4 −/− ) mice with or without B29 at the end of the trial. Scale bar, 100 μm. (B) NAFLD activity score. (C) Body weight. (D) Mass of epididymal, mesenteric, subcutaneous inguinal, and retroperitoneal fat pads of TLR4 −/− mice with or without B29. (E) Plasma insulin concentration before (fasting) and 30 min after oral glucose load in TLR4 −/− mice with or without B29. (F) ELISA of serum leptin in TLR4 −/− mice with or without B29 (after adjustment for body weight). (G) ELISA of serum LBP in TLR4 −/− mice with or without B29. (H) ELISA of serum amyloid A (SAA-3) in TLR4 −/− mice with or without B29. Data shown are means ± SEM ( n = 5 to 9). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.

    Techniques Used: H&E Stain, Mutagenesis, Activity Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

    mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
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    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
    94/100 stars

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    mouse lipopolysaccharide binding protein lbp  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein lbp
    Mouse Lipopolysaccharide Binding Protein Lbp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    • mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)
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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    mouse lipopolysaccharide binding protein lbp  (Hycult Biotech)
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    Hycult Biotech mouse lipopolysaccharide binding protein lbp
    Mouse Lipopolysaccharide Binding Protein Lbp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein lbp/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse lipopolysaccharide binding protein lbp - by Bioz Stars, 2023-06
    94/100 stars
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