mouse il 6 elisa kit  (BioLegend)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Mouse IL 6 ELISA MAX Standard
    Description:
    Mouse IL 6 ELISA MAX Standard Reactivity Mouse Apps ELISA Size 5 Plates
    Catalog Number:
    431301
    Price:
    225
    Applications:
    ELISA
    Conjugate:
    Standard
    Size:
    5 Plates
    Category:
    ELISA KIT
    Source:
    Rat
    Quantity:
    1
    Buy from Supplier


    Structured Review

    BioLegend mouse il 6 elisa kit
    Mouse IL 6 ELISA MAX Standard
    Mouse IL 6 ELISA MAX Standard Reactivity Mouse Apps ELISA Size 5 Plates
    https://www.bioz.com/result/mouse il 6 elisa kit/product/BioLegend
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 6 elisa kit - by Bioz Stars, 2020-09
    97/100 stars

    Images

    1) Product Images from "Gut microbial metabolites alter IgA immunity in type 1 diabetes"

    Article Title: Gut microbial metabolites alter IgA immunity in type 1 diabetes

    Journal: JCI Insight

    doi: 10.1172/jci.insight.135718

    Direct effect of acetate on B cells. Ex vivo splenic B cells were purified from specific pathogen–free NOD mice gavaged with acetate or water for 10 to 12 weeks. The purified B cells were stimulated in vitro in the presence of 10 Mm acetate with 20 μg/mL anti-CD40 mAb and 10 μg/mL LPS for 5 days. ( A ) IgA concentration in the culture supernatant of stimulated B cells was measured by ELISA ( n = 8–9). ( B ) Representative flow cytometric plots of intracellular IL-6 expression of B cells after acetate stimulation. ( C ) Summary of IL-6-expressing B cells. ( D ) Secreted IL-6, determined by ELISA, from the culture supernatant of B cells stimulated with acetate ( n = 6–7). ( E – H ) Gene expression of B cells, after acetate stimulation, was assessed by qPCR: Pst α ( E ), Pst2 β ( F ), Stat5b ( G ), and Irf4 ( H ). The expression levels were determined using the 2 −ΔΔCt method by normalizing the housekeeping gene Gapdh . Data combined from 2 independent experiments are presented as mean ± SEM and were analyzed using a 2-tailed Student’s t test ( A and C – H ).
    Figure Legend Snippet: Direct effect of acetate on B cells. Ex vivo splenic B cells were purified from specific pathogen–free NOD mice gavaged with acetate or water for 10 to 12 weeks. The purified B cells were stimulated in vitro in the presence of 10 Mm acetate with 20 μg/mL anti-CD40 mAb and 10 μg/mL LPS for 5 days. ( A ) IgA concentration in the culture supernatant of stimulated B cells was measured by ELISA ( n = 8–9). ( B ) Representative flow cytometric plots of intracellular IL-6 expression of B cells after acetate stimulation. ( C ) Summary of IL-6-expressing B cells. ( D ) Secreted IL-6, determined by ELISA, from the culture supernatant of B cells stimulated with acetate ( n = 6–7). ( E – H ) Gene expression of B cells, after acetate stimulation, was assessed by qPCR: Pst α ( E ), Pst2 β ( F ), Stat5b ( G ), and Irf4 ( H ). The expression levels were determined using the 2 −ΔΔCt method by normalizing the housekeeping gene Gapdh . Data combined from 2 independent experiments are presented as mean ± SEM and were analyzed using a 2-tailed Student’s t test ( A and C – H ).

    Techniques Used: Ex Vivo, Purification, Mouse Assay, In Vitro, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

    2) Product Images from "Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice"

    Article Title: Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice

    Journal: Cytotechnology

    doi: 10.1007/s10616-019-00296-4

    Effect of lysozyme on inflammatory cytokine levels in the liver. IL-6 ( a ) and TNF-α ( b ) levels in the liver analyzed using ELISA kits. Opened circle, intact group; closed circle, control group; opened triangle, low-dose group; shaded triangle, middle-dose group; closed triangle, high-dose group. Data are represented as mean ± standard deviation ( n = 7). ** p
    Figure Legend Snippet: Effect of lysozyme on inflammatory cytokine levels in the liver. IL-6 ( a ) and TNF-α ( b ) levels in the liver analyzed using ELISA kits. Opened circle, intact group; closed circle, control group; opened triangle, low-dose group; shaded triangle, middle-dose group; closed triangle, high-dose group. Data are represented as mean ± standard deviation ( n = 7). ** p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Effect of lysozyme on inflammatory cytokine levels in the spleen. IL-6 ( a ) and TNF-α ( b ) levels in the spleen analyzed using ELISA kits. Opened circle, intact group; closed circle, control group; opened triangle, low-dose group; shaded triangle, middle-dose group; closed triangle, high-dose group. Data are represented as mean ± standard deviation ( n = 7). * p
    Figure Legend Snippet: Effect of lysozyme on inflammatory cytokine levels in the spleen. IL-6 ( a ) and TNF-α ( b ) levels in the spleen analyzed using ELISA kits. Opened circle, intact group; closed circle, control group; opened triangle, low-dose group; shaded triangle, middle-dose group; closed triangle, high-dose group. Data are represented as mean ± standard deviation ( n = 7). * p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Effect of lysozyme on the amounts of inflammatory cytokines in serum. IL-6 ( a ) and TNF-α ( b ) levels in the serum analyzed using ELISA kits. Opened circle, intact group; closed circle, control group; opened triangle, low-dose group; shaded triangle, middle-dose group; closed triangle, high-dose group. Data are represented as mean ± standard deviation ( n = 7). * p
    Figure Legend Snippet: Effect of lysozyme on the amounts of inflammatory cytokines in serum. IL-6 ( a ) and TNF-α ( b ) levels in the serum analyzed using ELISA kits. Opened circle, intact group; closed circle, control group; opened triangle, low-dose group; shaded triangle, middle-dose group; closed triangle, high-dose group. Data are represented as mean ± standard deviation ( n = 7). * p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inhibition of Mast Cell Function and Proliferation by mTOR Activator MHY1485
    Article Snippet: .. To measure secreted cytokines, supernatants were harvested after 6 h and tested for their levels of cytokines using ELISAs with Mouse IL-6 and TNF-α ELISA MAX Deluxe kits (BioLegend, San Diego, CA, USA) according to the manufacturer's instructions. .. Cell number monitoring was assessed using a Cell Counting Kit-8 incorporating WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) (Dojindo Molecular Technologies, Rockville, MD, USA).

    Article Title: Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice
    Article Snippet: .. The levels of IL-1β, IL-6, CXCL1, TNF-α, and IL-10 were measured in BAL supernatant by using commercially available ELISA kits according to the manufacturer's instructions: IL-1β (#43601; BioLegend), IL-6 (#431301; BioLegend), CXCL1 (#DY453; R & D), TNF-α (#430901; BioLegend), IL-10 (#431411; BioLegend). .. Paraffin sections (5 μm) were placed on slides and stained with hematoxylin and eosin to quantify the number of neutrophils in the lung parenchyma.

    Article Title: Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice
    Article Snippet: .. The concentrations of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in samples were measured by enzyme-linked immunosorbent assay (ELISA) using mouse IL-6 ELISA kit (BioLegend, San Diego, CA, USA) and mouse TNF-α ELISA kit (eBioscience, San Diego, CA, USA), respectively, according to the manufacturer’s instructions. ..

    Article Title: Early Secreted Antigenic Target of 6-kDa of Mycobacterium tuberculosis Stimulates IL-6 Production by Macrophages through Activation of STAT3
    Article Snippet: .. Determination of IL-6 concentration IL-6 levels in the culture supernatants were determined by Human IL-6 ELISA MAXTM Standard or mouse IL-6 ELISA MAXTM Standard (both from Biolegend) following the manufacturer’s instructions. ..

    Article Title: Gut microbial metabolites alter IgA immunity in type 1 diabetes
    Article Snippet: .. Cytokine ELISA.Murine IL-6 was measured using the Mouse IL-6 ELISA kit following the manufacturer’s instructions (BioLegend). .. Real-time qPCR.

    Article Title: Mycolactone displays anti-inflammatory effects on the nervous system
    Article Snippet: .. The release of CCL2, IL-6 and TNF-α into culture supernatant was assessed by ELISA using respectively the mouse CCL2 Uncoated ELISA kit (ThermoFisher scientific, 88-7391-22) and the mouse IL-6 and TNF-α ELISA MAX kits (Biolegend, 431301 and 430904). .. For IL-6 ELISA assay on SCs, SCs were seeded at 2 x 104 in 24 well plates coated with poly-L-Lysin.

    Article Title: Platelets convert peripheral blood circulating monocytes to regulatory cells via immunoglobulin G and activating-type Fcγ receptors
    Article Snippet: .. ELISA ELISAs were performed to measure human IL-1β, human IL-10, human IL-12, mouse IL-6, mouse IL-10, and mouse IL-12 using Human IL-1β ELISA Ready-SET-Go!, Human IL-10 ELISA Ready-SET-Go!, Human IL-12 ELISA Ready-SET-Go! (eBioscience, San Diego, CA), Mouse IL-6 ELISA MAX standard, Mouse IL-10 ELISA MAX standard, and Mouse IL-12 ELISA MAX standard, respectively (BioLegend). .. All ELISAs were performed using 96-well half-area microplates (Greiner) according to the manufacturer's directions.

    Concentration Assay:

    Article Title: Early Secreted Antigenic Target of 6-kDa of Mycobacterium tuberculosis Stimulates IL-6 Production by Macrophages through Activation of STAT3
    Article Snippet: .. Determination of IL-6 concentration IL-6 levels in the culture supernatants were determined by Human IL-6 ELISA MAXTM Standard or mouse IL-6 ELISA MAXTM Standard (both from Biolegend) following the manufacturer’s instructions. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    BioLegend tnfα elisa kit
    In vivo treatment with GSC839 does not induce inflammation. (A) Production of inflammatory cytokines. C57BL/6 mice were subcutaneously immunized with 2.5 µg ovalbumin together with indicated amounts of GSC839, CpG oligo, or alum. The levels of serum <t>TNFα</t> and IL-6 24 h after immunization were measured by <t>ELISA.</t> Data were analyzed by Kruskal–Wallis test and Steel analysis was applied as post hoc analysis. * P
    Tnfα Elisa Kit, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfα elisa kit/product/BioLegend
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    tnfα elisa kit - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    97
    BioLegend mouse il 6
    Expression of A-FABP in macrophages promotes <t>IL-6</t> production
    Mouse Il 6, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 6/product/BioLegend
    Average 97 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    mouse il 6 - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    92
    BioLegend elisa kit
    Purification and functional characterization of eCRT. Purified rCRT/39-272, rCRT, eCRT and nCRT (lanes 1–4, respectively) were separated by SDS-PAGE ( A ) and detected by rabbit anti-CRT polyclonal antibodies in Western-blot ( B ). Mouse peritoneal macrophages were stimulated with these CRT preparations of increasing concentrations for 24 h, followed by quantitation of NO ( C ) and <t>TNF-α</t> ( D ) in the culture supernatant using Griess Reagent or <t>ELISA,</t> respectively. Macrophages stimulated with LPS (1 ng/mL) or rEGFP (1 µg/mL) were included as controls.
    Elisa Kit, supplied by BioLegend, used in various techniques. Bioz Stars score: 92/100, based on 291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/BioLegend
    Average 92 stars, based on 291 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    In vivo treatment with GSC839 does not induce inflammation. (A) Production of inflammatory cytokines. C57BL/6 mice were subcutaneously immunized with 2.5 µg ovalbumin together with indicated amounts of GSC839, CpG oligo, or alum. The levels of serum TNFα and IL-6 24 h after immunization were measured by ELISA. Data were analyzed by Kruskal–Wallis test and Steel analysis was applied as post hoc analysis. * P

    Journal: Frontiers in Immunology

    Article Title: CD22-Binding Synthetic Sialosides Regulate B Lymphocyte Proliferation Through CD22 Ligand-Dependent and Independent Pathways, and Enhance Antibody Production in Mice

    doi: 10.3389/fimmu.2018.00820

    Figure Lengend Snippet: In vivo treatment with GSC839 does not induce inflammation. (A) Production of inflammatory cytokines. C57BL/6 mice were subcutaneously immunized with 2.5 µg ovalbumin together with indicated amounts of GSC839, CpG oligo, or alum. The levels of serum TNFα and IL-6 24 h after immunization were measured by ELISA. Data were analyzed by Kruskal–Wallis test and Steel analysis was applied as post hoc analysis. * P

    Article Snippet: TNFα and IL-6 were measured by TNFα ELISA kit and IL-6 ELISA kit (BioLegend), respectively, according to the manufacture’s protocol.

    Techniques: In Vivo, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Expression of A-FABP in macrophages promotes IL-6 production

    Journal: Cancer research

    Article Title: Expression of adipocyte/macrophage fatty acid binding protein in tumor associated macrophages promotes breast cancer progression

    doi: 10.1158/0008-5472.CAN-17-2465

    Figure Lengend Snippet: Expression of A-FABP in macrophages promotes IL-6 production

    Article Snippet: ELISA kits for mouse IL-6, IL-10 or TNFα (Biolegend) were used to detect respective cytokines in mouse serum and cell cultural supernatants according to the manufacturer’s instruction.

    Techniques: Expressing

    A-FABP promotes IL-6 production via the NFκB/ miR-29b pathway in macrophages

    Journal: Cancer research

    Article Title: Expression of adipocyte/macrophage fatty acid binding protein in tumor associated macrophages promotes breast cancer progression

    doi: 10.1158/0008-5472.CAN-17-2465

    Figure Lengend Snippet: A-FABP promotes IL-6 production via the NFκB/ miR-29b pathway in macrophages

    Article Snippet: ELISA kits for mouse IL-6, IL-10 or TNFα (Biolegend) were used to detect respective cytokines in mouse serum and cell cultural supernatants according to the manufacturer’s instruction.

    Techniques:

    Effects of MHY1485 on FcεRI-mediated degranulation and cytokine production in mast cells. BMMCs were sensitized for 5 h, incubated with the indicated concentration of MHY1485 for 1 h, and then either unstimulated (unstim) or stimulated with the DNP-HSA Ag. (A) Time-course immunoblot analysis for mTOR signaling, with or without MHY1485, following Ag stimulation. Band densities of pS6K on Thr389 and pAkt on Ser473 were normalized to their total protein expression from the results of 3 independent experiments. AU represents arbitrary unit. (B) Degranulation was assessed by measuring β-hexosaminidase release 30 min after stimulation. (C) The levels of IL-6 and TNF-α proteins in the media were analyzed using ELISA 6 h after stimulation. (D) qRT-PCR analysis for FcεRI-mediated induction of Il6 and Tfna mRNA was carried out 1 h after Ag stimulation. Bar graphs are shown as mean±standard error of the mean of triplicates and are representative of 3 independent experiments. A p-value of less than 0.05 between stimulated groups with and without MHY1485 treatment is judged significant and indicated. pS6K, phospho-S6K; Thr389, threonine 389; pAkt, phospho-Akt; Ser473, serine 473; qRT-PCR, quantitative real-time PCR. * p

    Journal: Immune Network

    Article Title: Inhibition of Mast Cell Function and Proliferation by mTOR Activator MHY1485

    doi: 10.4110/in.2018.18.e18

    Figure Lengend Snippet: Effects of MHY1485 on FcεRI-mediated degranulation and cytokine production in mast cells. BMMCs were sensitized for 5 h, incubated with the indicated concentration of MHY1485 for 1 h, and then either unstimulated (unstim) or stimulated with the DNP-HSA Ag. (A) Time-course immunoblot analysis for mTOR signaling, with or without MHY1485, following Ag stimulation. Band densities of pS6K on Thr389 and pAkt on Ser473 were normalized to their total protein expression from the results of 3 independent experiments. AU represents arbitrary unit. (B) Degranulation was assessed by measuring β-hexosaminidase release 30 min after stimulation. (C) The levels of IL-6 and TNF-α proteins in the media were analyzed using ELISA 6 h after stimulation. (D) qRT-PCR analysis for FcεRI-mediated induction of Il6 and Tfna mRNA was carried out 1 h after Ag stimulation. Bar graphs are shown as mean±standard error of the mean of triplicates and are representative of 3 independent experiments. A p-value of less than 0.05 between stimulated groups with and without MHY1485 treatment is judged significant and indicated. pS6K, phospho-S6K; Thr389, threonine 389; pAkt, phospho-Akt; Ser473, serine 473; qRT-PCR, quantitative real-time PCR. * p

    Article Snippet: To measure secreted cytokines, supernatants were harvested after 6 h and tested for their levels of cytokines using ELISAs with Mouse IL-6 and TNF-α ELISA MAX Deluxe kits (BioLegend, San Diego, CA, USA) according to the manufacturer's instructions.

    Techniques: Incubation, Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Purification and functional characterization of eCRT. Purified rCRT/39-272, rCRT, eCRT and nCRT (lanes 1–4, respectively) were separated by SDS-PAGE ( A ) and detected by rabbit anti-CRT polyclonal antibodies in Western-blot ( B ). Mouse peritoneal macrophages were stimulated with these CRT preparations of increasing concentrations for 24 h, followed by quantitation of NO ( C ) and TNF-α ( D ) in the culture supernatant using Griess Reagent or ELISA, respectively. Macrophages stimulated with LPS (1 ng/mL) or rEGFP (1 µg/mL) were included as controls.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Aberrant Glycosylation Augments the Immuno-Stimulatory Activities of Soluble Calreticulin

    doi: 10.3390/molecules23030523

    Figure Lengend Snippet: Purification and functional characterization of eCRT. Purified rCRT/39-272, rCRT, eCRT and nCRT (lanes 1–4, respectively) were separated by SDS-PAGE ( A ) and detected by rabbit anti-CRT polyclonal antibodies in Western-blot ( B ). Mouse peritoneal macrophages were stimulated with these CRT preparations of increasing concentrations for 24 h, followed by quantitation of NO ( C ) and TNF-α ( D ) in the culture supernatant using Griess Reagent or ELISA, respectively. Macrophages stimulated with LPS (1 ng/mL) or rEGFP (1 µg/mL) were included as controls.

    Article Snippet: After stimulation with eCRT, tun-eCRT, rCRT and EGFP for 24 h, TNF-α and IL-6 in supernatant was determined by ELISA kit (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions.

    Techniques: Purification, Functional Assay, SDS Page, Western Blot, Quantitation Assay, Enzyme-linked Immunosorbent Assay