mouse il 6 elisa kit  (Boster Bio)


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    Structured Review

    Boster Bio mouse il 6 elisa kit
    Macrophage activation caused inflammation of lung tissue in response to PM 2 . 5 . (A) Immunohistochemistry detection of the macrophage marker F4/80 in mouse lung tissues. Scale bar = 10 μm. (B) Pathological changes in the lung tissues. Scale bar = 10 μm. (C , D) Western blot analysis of TNF-α and IL-1β levels in the lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (E) <t>ELISA</t> analysis of <t>IL-6</t> in serum ( n = 5). (F) Real-time quantitative PCR analysis of IL-6, TNF-α, and IL-1β in the lung ( n = 8). (G , H) Western blot analysis of the P-PI3K, P-AKT, and NF-κB in mouse lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 μm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. * P
    Mouse Il 6 Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 6 elisa kit/product/Boster Bio
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mouse il 6 elisa kit - by Bioz Stars, 2022-09
    94/100 stars

    Images

    1) Product Images from "lncRNA Gm16410 Mediates PM2.5-Induced Macrophage Activation via PI3K/AKT Pathway"

    Article Title: lncRNA Gm16410 Mediates PM2.5-Induced Macrophage Activation via PI3K/AKT Pathway

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2021.618045

    Macrophage activation caused inflammation of lung tissue in response to PM 2 . 5 . (A) Immunohistochemistry detection of the macrophage marker F4/80 in mouse lung tissues. Scale bar = 10 μm. (B) Pathological changes in the lung tissues. Scale bar = 10 μm. (C , D) Western blot analysis of TNF-α and IL-1β levels in the lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (E) ELISA analysis of IL-6 in serum ( n = 5). (F) Real-time quantitative PCR analysis of IL-6, TNF-α, and IL-1β in the lung ( n = 8). (G , H) Western blot analysis of the P-PI3K, P-AKT, and NF-κB in mouse lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 μm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. * P
    Figure Legend Snippet: Macrophage activation caused inflammation of lung tissue in response to PM 2 . 5 . (A) Immunohistochemistry detection of the macrophage marker F4/80 in mouse lung tissues. Scale bar = 10 μm. (B) Pathological changes in the lung tissues. Scale bar = 10 μm. (C , D) Western blot analysis of TNF-α and IL-1β levels in the lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (E) ELISA analysis of IL-6 in serum ( n = 5). (F) Real-time quantitative PCR analysis of IL-6, TNF-α, and IL-1β in the lung ( n = 8). (G , H) Western blot analysis of the P-PI3K, P-AKT, and NF-κB in mouse lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 μm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. * P

    Techniques Used: Activation Assay, Immunohistochemistry, Marker, Western Blot, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

    2) Product Images from "Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells"

    Article Title: Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells

    Journal: BioMed Research International

    doi: 10.1155/2015/607692

    MCPIP1 overexpression decreased the secretion of IL-6, IL-1 β , and IL-10 in macrophage cells stimulated with LPS. (a) MCPIP1 overexpression in macrophage cells stimulated with LPS. (b) Experimental design of cell stimulation experiments. (c) Secretion of IL-6 from RAW264.7 cells stimulated with E. coli LPS. (d) Secretion of IL-1 β from RAW264.7 cells stimulated with E. coli LPS. (e) Secretion of IL-10 from RAW264.7 cells stimulated with E. coli LPS.
    Figure Legend Snippet: MCPIP1 overexpression decreased the secretion of IL-6, IL-1 β , and IL-10 in macrophage cells stimulated with LPS. (a) MCPIP1 overexpression in macrophage cells stimulated with LPS. (b) Experimental design of cell stimulation experiments. (c) Secretion of IL-6 from RAW264.7 cells stimulated with E. coli LPS. (d) Secretion of IL-1 β from RAW264.7 cells stimulated with E. coli LPS. (e) Secretion of IL-10 from RAW264.7 cells stimulated with E. coli LPS.

    Techniques Used: Over Expression, Cell Stimulation

    3) Product Images from "Trichodimerol inhibits inflammation through suppression of the nuclear transcription factor-kappaB/NOD-like receptor thermal protein domain associated protein 3 signaling pathway"

    Article Title: Trichodimerol inhibits inflammation through suppression of the nuclear transcription factor-kappaB/NOD-like receptor thermal protein domain associated protein 3 signaling pathway

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.999996

    The effect of trichodimerol on inflammation. (A) Nitric oxide (NO) production in the supernatant of RAW264.7 macrophages after trichodimerol administration. (B) RAW264.7 macrophages were treated with different concentrations of trichodimerol and induced by lipopolysaccharide (LPS). The expression of tumor necrosis factor (TNF)-α and IL-6 in the supernatant was detected by ELISA. (C) mRNA expression levels of IL-6 and TNF-α in RAW264.7 macrophages in different treatment groups. All data are expressed as the mean ± SD. # p
    Figure Legend Snippet: The effect of trichodimerol on inflammation. (A) Nitric oxide (NO) production in the supernatant of RAW264.7 macrophages after trichodimerol administration. (B) RAW264.7 macrophages were treated with different concentrations of trichodimerol and induced by lipopolysaccharide (LPS). The expression of tumor necrosis factor (TNF)-α and IL-6 in the supernatant was detected by ELISA. (C) mRNA expression levels of IL-6 and TNF-α in RAW264.7 macrophages in different treatment groups. All data are expressed as the mean ± SD. # p

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

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    Boster Bio mouse il 6 elisa kit picokine
    Sleep deprivation induced cognitive impairment. (A) The escape latency during 5 days of training and (B) The trajectory of the last experiment in a Morris water maze assay was used to measure the learning and memory ability. (C) The expression of inflammatory cytokines <t>(IL-6,</t> IL-1β, TNF-α) was detected by an <t>ELISA</t> assay kit. Data are mean ± SD of at least three duplicate experiments. * p
    Mouse Il 6 Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 6 elisa kit picokine/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 6 elisa kit picokine - by Bioz Stars, 2022-09
    95/100 stars
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    94
    Boster Bio elisa kits
    Andro treatment decreased the expression levels of pro-inflammatory cytokines after TBI. The mRNA levels of <t>TNF-α</t> (A) , IL-1β (B) and IL-6 (C) in peri-contusive cortex were determined by QPCR at 6 h after TBI. The protein levels of TNF-α (D) , IL-1β (E) and IL-6 (F) in peri-contusive cortex were measured by <t>ELISA</t> at 24 h after TBI. All data are presented as the mean ± SD. ∗ p
    Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Sleep deprivation induced cognitive impairment. (A) The escape latency during 5 days of training and (B) The trajectory of the last experiment in a Morris water maze assay was used to measure the learning and memory ability. (C) The expression of inflammatory cytokines (IL-6, IL-1β, TNF-α) was detected by an ELISA assay kit. Data are mean ± SD of at least three duplicate experiments. * p

    Journal: Frontiers in Neurology

    Article Title: Sleep Deprivation Induces Cognitive Impairment by Increasing Blood-Brain Barrier Permeability via CD44

    doi: 10.3389/fneur.2020.563916

    Figure Lengend Snippet: Sleep deprivation induced cognitive impairment. (A) The escape latency during 5 days of training and (B) The trajectory of the last experiment in a Morris water maze assay was used to measure the learning and memory ability. (C) The expression of inflammatory cytokines (IL-6, IL-1β, TNF-α) was detected by an ELISA assay kit. Data are mean ± SD of at least three duplicate experiments. * p

    Article Snippet: IL-6 (EK0411, Boster Biological Technology Co. Ltd), IL-1β (EK0394, Boster Biological Technology Co. Ltd), and tumor necrosis factor (TNF)-α (EK0527, Boster Biological Technology Co. Ltd) ELISA kits were used to measure the levels of IL-6, IL-1β, and TNF-α in the mouse hippocampus tissue following the instructions from the manufacturer.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay

    Macrophage activation caused inflammation of lung tissue in response to PM 2 . 5 . (A) Immunohistochemistry detection of the macrophage marker F4/80 in mouse lung tissues. Scale bar = 10 μm. (B) Pathological changes in the lung tissues. Scale bar = 10 μm. (C , D) Western blot analysis of TNF-α and IL-1β levels in the lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (E) ELISA analysis of IL-6 in serum ( n = 5). (F) Real-time quantitative PCR analysis of IL-6, TNF-α, and IL-1β in the lung ( n = 8). (G , H) Western blot analysis of the P-PI3K, P-AKT, and NF-κB in mouse lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 μm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. * P

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: lncRNA Gm16410 Mediates PM2.5-Induced Macrophage Activation via PI3K/AKT Pathway

    doi: 10.3389/fcell.2021.618045

    Figure Lengend Snippet: Macrophage activation caused inflammation of lung tissue in response to PM 2 . 5 . (A) Immunohistochemistry detection of the macrophage marker F4/80 in mouse lung tissues. Scale bar = 10 μm. (B) Pathological changes in the lung tissues. Scale bar = 10 μm. (C , D) Western blot analysis of TNF-α and IL-1β levels in the lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (E) ELISA analysis of IL-6 in serum ( n = 5). (F) Real-time quantitative PCR analysis of IL-6, TNF-α, and IL-1β in the lung ( n = 8). (G , H) Western blot analysis of the P-PI3K, P-AKT, and NF-κB in mouse lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 μm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. * P

    Article Snippet: Mouse IL-6 ELISA kit was purchased from Boster (Wuhan, China).

    Techniques: Activation Assay, Immunohistochemistry, Marker, Western Blot, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

    Andro treatment decreased the expression levels of pro-inflammatory cytokines after TBI. The mRNA levels of TNF-α (A) , IL-1β (B) and IL-6 (C) in peri-contusive cortex were determined by QPCR at 6 h after TBI. The protein levels of TNF-α (D) , IL-1β (E) and IL-6 (F) in peri-contusive cortex were measured by ELISA at 24 h after TBI. All data are presented as the mean ± SD. ∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling

    doi: 10.3389/fnins.2018.00657

    Figure Lengend Snippet: Andro treatment decreased the expression levels of pro-inflammatory cytokines after TBI. The mRNA levels of TNF-α (A) , IL-1β (B) and IL-6 (C) in peri-contusive cortex were determined by QPCR at 6 h after TBI. The protein levels of TNF-α (D) , IL-1β (E) and IL-6 (F) in peri-contusive cortex were measured by ELISA at 24 h after TBI. All data are presented as the mean ± SD. ∗ p

    Article Snippet: The expression of TNF-α, IL-1β and IL-6 were measured with ELISA kits (Boster Biosciences Co., Wuhan, China).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    In vivo anti-inflammatory assessments. (A) Treatment schedule. (B) TNF-α level. (C) IL-6 level. (D) Total cells counts. (E) Neutrophils counts. # p

    Journal: Drug Delivery

    Article Title: Targeting delivery of simvastatin using ICAM-1 antibody-conjugated nanostructured lipid carriers for acute lung injury therapy

    doi: 10.1080/10717544.2016.1259369

    Figure Lengend Snippet: In vivo anti-inflammatory assessments. (A) Treatment schedule. (B) TNF-α level. (C) IL-6 level. (D) Total cells counts. (E) Neutrophils counts. # p

    Article Snippet: TNF-α, IL-6 ELISA kits were from Boster Co., Ltd. (Wuhan, China).

    Techniques: In Vivo