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Bio-Rad mouse il 15 kit
Running endurance and <t>IL-15</t> expression. A, Control mice ran longer than IL-15–KO mice in a run-to-exhaustion test. *, Significant difference between control and IL-15—KO mice at P
Mouse Il 15 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse il 15 kit/product/Bio-Rad
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse il 15 kit - by Bioz Stars, 2021-09
86/100 stars

Images

1) Product Images from "IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice"

Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

Journal: Endocrinology

doi: 10.1210/en.2013-1645

Running endurance and IL-15 expression. A, Control mice ran longer than IL-15–KO mice in a run-to-exhaustion test. *, Significant difference between control and IL-15—KO mice at P
Figure Legend Snippet: Running endurance and IL-15 expression. A, Control mice ran longer than IL-15–KO mice in a run-to-exhaustion test. *, Significant difference between control and IL-15—KO mice at P

Techniques Used: Expressing, Mouse Assay

Effects of IL-15 injection on acute expression of pro-oxidative mediators in gastrocnemius muscles of IL-15–KO mice. A and B, Expression of PPARδ, SIRT1, PGC-1α, and PGC-1β mRNA in gastrocnemius muscles of IL-15–KO
Figure Legend Snippet: Effects of IL-15 injection on acute expression of pro-oxidative mediators in gastrocnemius muscles of IL-15–KO mice. A and B, Expression of PPARδ, SIRT1, PGC-1α, and PGC-1β mRNA in gastrocnemius muscles of IL-15–KO

Techniques Used: Injection, Expressing, Mouse Assay, Pyrolysis Gas Chromatography

Postexhaustive exercise induction of PGC-1α and PGC-1β. A and C, PGC-1α (A) and PGC-1β (C) mRNA expression in gastrocnemius muscles of control and IL-15–KO sedentary mice (SED) or at different time points (immediately
Figure Legend Snippet: Postexhaustive exercise induction of PGC-1α and PGC-1β. A and C, PGC-1α (A) and PGC-1β (C) mRNA expression in gastrocnemius muscles of control and IL-15–KO sedentary mice (SED) or at different time points (immediately

Techniques Used: Pyrolysis Gas Chromatography, Expressing, Mouse Assay

Postexhaustive exercise induction of PPARδ and SIRT1. A and C, PPARδ (A) and SIRT1(C) gastrocnemius muscle mRNA expression in control and IL-15–KO sedentary (SED) mice at different time points (immediately [IMMED], 30 minutes,
Figure Legend Snippet: Postexhaustive exercise induction of PPARδ and SIRT1. A and C, PPARδ (A) and SIRT1(C) gastrocnemius muscle mRNA expression in control and IL-15–KO sedentary (SED) mice at different time points (immediately [IMMED], 30 minutes,

Techniques Used: Expressing, Mouse Assay

Postexercise muscle expression of IL-15Rα and circulating IL-15 levels in IL-15Rα–KO mice. A, IL-15Rα mRNA expression in gastrocnemius muscles of sedentary (SED) control and IL-15–KO mice and at intervals after
Figure Legend Snippet: Postexercise muscle expression of IL-15Rα and circulating IL-15 levels in IL-15Rα–KO mice. A, IL-15Rα mRNA expression in gastrocnemius muscles of sedentary (SED) control and IL-15–KO mice and at intervals after

Techniques Used: Expressing, Mouse Assay

Body composition in control and IL-15–KO mice
Figure Legend Snippet: Body composition in control and IL-15–KO mice

Techniques Used: Mouse Assay

2) Product Images from "IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice"

Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

Journal: Endocrinology

doi: 10.1210/en.2012-1773

Nuclear and mitochondrial (Mt) DNA, and markers of Mt activity, in selected tissues of control (CON) and IL-15 Tg (TG) mice. A, Total (predominately nuclear) DNA per milligram tissue; B, Mt DNA per tissue weight; C, CPT1B mRNA expression; D, UCP2 mRNA
Figure Legend Snippet: Nuclear and mitochondrial (Mt) DNA, and markers of Mt activity, in selected tissues of control (CON) and IL-15 Tg (TG) mice. A, Total (predominately nuclear) DNA per milligram tissue; B, Mt DNA per tissue weight; C, CPT1B mRNA expression; D, UCP2 mRNA

Techniques Used: Activity Assay, Mouse Assay, Expressing

Expression of mRNA markers for muscle phenotype in control (CON) and IL-15 Tg (TG) mice in mixed gastrocnemius muscle (GASTROC), EDL, and soleus muscle. A, Troponin I slow; B, Troponin I fast; C, MHC-1, typically expressed in slow/oxidative fibers; D,
Figure Legend Snippet: Expression of mRNA markers for muscle phenotype in control (CON) and IL-15 Tg (TG) mice in mixed gastrocnemius muscle (GASTROC), EDL, and soleus muscle. A, Troponin I slow; B, Troponin I fast; C, MHC-1, typically expressed in slow/oxidative fibers; D,

Techniques Used: Expressing, Mouse Assay

Expression of selected mRNA species involved in fat oxidation in several tissues involved in energy metabolism in untrained control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between
Figure Legend Snippet: Expression of selected mRNA species involved in fat oxidation in several tissues involved in energy metabolism in untrained control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between

Techniques Used: Expressing, Mouse Assay

Phenotypic characteristics of IL-15 Tg and control mice
Figure Legend Snippet: Phenotypic characteristics of IL-15 Tg and control mice

Techniques Used: Mouse Assay

Energy metabolism, activity, and running endurance in control (CON) and IL-15 Tg (TG) mice. A, RER was significantly lower in TG mice, indicating preferential use of fat for energy metabolism. B, Food intake (FI) did not differ between genotypes. C and
Figure Legend Snippet: Energy metabolism, activity, and running endurance in control (CON) and IL-15 Tg (TG) mice. A, RER was significantly lower in TG mice, indicating preferential use of fat for energy metabolism. B, Food intake (FI) did not differ between genotypes. C and

Techniques Used: Activity Assay, Mouse Assay

Differential expression of PGC-1α, PPARγ, and PDK4 in liver and sc fat of control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between genotypes (B, D, and F). A and B,
Figure Legend Snippet: Differential expression of PGC-1α, PPARγ, and PDK4 in liver and sc fat of control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between genotypes (B, D, and F). A and B,

Techniques Used: Expressing, Pyrolysis Gas Chromatography, Mouse Assay

Protein levels of intracellular mediators of oxidative metabolism, analyzed by Western blots, in (CON) and IL-15 Tg (TG) mouse EDL and soleus muscles. A, SIRT1; B, PPARδ; C, PGC-1β; D, PGC-1α. Bars represent means ± sem
Figure Legend Snippet: Protein levels of intracellular mediators of oxidative metabolism, analyzed by Western blots, in (CON) and IL-15 Tg (TG) mouse EDL and soleus muscles. A, SIRT1; B, PPARδ; C, PGC-1β; D, PGC-1α. Bars represent means ± sem

Techniques Used: Western Blot, Pyrolysis Gas Chromatography

IL-15Rα expression in control (CON) and IL-15 Tg (TG) mice. A, IL-15Rα mRNA expression (n = 6–9 mice per group); B, IL-15Rα protein levels in muscle tissue, determined by Western blot (n = 4 CON and 5 TG). Signal in each
Figure Legend Snippet: IL-15Rα expression in control (CON) and IL-15 Tg (TG) mice. A, IL-15Rα mRNA expression (n = 6–9 mice per group); B, IL-15Rα protein levels in muscle tissue, determined by Western blot (n = 4 CON and 5 TG). Signal in each

Techniques Used: Expressing, Mouse Assay, Western Blot

Related Articles

Expressing:

Article Title: Schistosoma Japonicum infection in Treg-specific USP21 knock-out mice
Article Snippet: .. The expression of USP21, IL-10, IL-17, FOXP3 in liver and spleen, and α-SMA, collagenI, collagen III in the liver were detected by iTaq™ Universal SYBR® Green super mixture (1725121, bio-rad) and fluorescence quantitative PCR 7500 system (applied biosystems, USA), and quantitative analysis was carried out by 2-ΔΔCt method with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control. ..

SYBR Green Assay:

Article Title: Schistosoma Japonicum infection in Treg-specific USP21 knock-out mice
Article Snippet: .. The expression of USP21, IL-10, IL-17, FOXP3 in liver and spleen, and α-SMA, collagenI, collagen III in the liver were detected by iTaq™ Universal SYBR® Green super mixture (1725121, bio-rad) and fluorescence quantitative PCR 7500 system (applied biosystems, USA), and quantitative analysis was carried out by 2-ΔΔCt method with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control. ..

Fluorescence:

Article Title: Schistosoma Japonicum infection in Treg-specific USP21 knock-out mice
Article Snippet: .. The expression of USP21, IL-10, IL-17, FOXP3 in liver and spleen, and α-SMA, collagenI, collagen III in the liver were detected by iTaq™ Universal SYBR® Green super mixture (1725121, bio-rad) and fluorescence quantitative PCR 7500 system (applied biosystems, USA), and quantitative analysis was carried out by 2-ΔΔCt method with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control. ..

Real-time Polymerase Chain Reaction:

Article Title: Schistosoma Japonicum infection in Treg-specific USP21 knock-out mice
Article Snippet: .. The expression of USP21, IL-10, IL-17, FOXP3 in liver and spleen, and α-SMA, collagenI, collagen III in the liver were detected by iTaq™ Universal SYBR® Green super mixture (1725121, bio-rad) and fluorescence quantitative PCR 7500 system (applied biosystems, USA), and quantitative analysis was carried out by 2-ΔΔCt method with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control. ..

Cytokine Assay:

Article Title: Histopathological Evaluation of the Diversity of Cells Susceptible to H5N1 Virulent Avian Influenza Virus
Article Snippet: .. The resulting homogenates were assessed for cytokines and chemokines using Bio-Plex cytokine assay kits (Bio-Rad Laboratories), according to the manufacturer’s instructions. ..

Intra Assay:

Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice
Article Snippet: .. Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad). ..

Protein Array:

Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice
Article Snippet: .. Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad). ..

Multiplex Assay:

Article Title: Alcohol enhances type 1 interferon-α production and mortality in young mice infected with Mycobacterium tuberculosis
Article Snippet: .. Measurement of cytokine production by multiplex ELISA In the lung homogenates, the following 27 cytokines and chemokines were measured using a multiplex ELISA kit (M60009RDPD, Bio-Rad). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Alcohol enhances type 1 interferon-α production and mortality in young mice infected with Mycobacterium tuberculosis
Article Snippet: .. Measurement of cytokine production by multiplex ELISA In the lung homogenates, the following 27 cytokines and chemokines were measured using a multiplex ELISA kit (M60009RDPD, Bio-Rad). ..

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    Bio-Rad mouse il 15 kit
    Running endurance and <t>IL-15</t> expression. A, Control mice ran longer than IL-15–KO mice in a run-to-exhaustion test. *, Significant difference between control and IL-15—KO mice at P
    Mouse Il 15 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 15 kit/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 15 kit - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    80
    Bio-Rad mouse il 15 assay kits
    Proposed interplay between <t>IL-15</t> and TNF-α signaling in skeletal muscle. Binding of TNF-α to TNF-R1 triggers the extrinsic pathway of apoptosis. Chronic IL-15 stimulation may result in downregulation of TNF-R1 and subsequent prevention of TNF-α-mediated apoptosis (solid line). Actions of IL-15 may be transduced by its cell surface trimeric receptor. IL-15 may also interact with IL-15Rα on the plasma membrane and be presented to adjacent cells (or portions of a muscle fiber) expressing the γ c /IL-2Rβ heterodimer. Soluble IL-15Rα (sIL-15Rα) may be important for secretion, stabilization and activity of IL-15. Additionally, in the presence of an acute apoptotic stimulus, IL-15Rα might counteract DISC assembly and promote the production of anti-apoptotic proteins such as FLIPs and cIAPs via NF-κB activation (dashed lines).
    Mouse Il 15 Assay Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 15 assay kits/product/Bio-Rad
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 15 assay kits - by Bioz Stars, 2021-09
    80/100 stars
      Buy from Supplier

    99
    Bio-Rad bio plex pro mouse kits
    Proposed interplay between <t>IL-15</t> and TNF-α signaling in skeletal muscle. Binding of TNF-α to TNF-R1 triggers the extrinsic pathway of apoptosis. Chronic IL-15 stimulation may result in downregulation of TNF-R1 and subsequent prevention of TNF-α-mediated apoptosis (solid line). Actions of IL-15 may be transduced by its cell surface trimeric receptor. IL-15 may also interact with IL-15Rα on the plasma membrane and be presented to adjacent cells (or portions of a muscle fiber) expressing the γ c /IL-2Rβ heterodimer. Soluble IL-15Rα (sIL-15Rα) may be important for secretion, stabilization and activity of IL-15. Additionally, in the presence of an acute apoptotic stimulus, IL-15Rα might counteract DISC assembly and promote the production of anti-apoptotic proteins such as FLIPs and cIAPs via NF-κB activation (dashed lines).
    Bio Plex Pro Mouse Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio plex pro mouse kits/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bio plex pro mouse kits - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Running endurance and IL-15 expression. A, Control mice ran longer than IL-15–KO mice in a run-to-exhaustion test. *, Significant difference between control and IL-15—KO mice at P

    Journal: Endocrinology

    Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

    doi: 10.1210/en.2013-1645

    Figure Lengend Snippet: Running endurance and IL-15 expression. A, Control mice ran longer than IL-15–KO mice in a run-to-exhaustion test. *, Significant difference between control and IL-15—KO mice at P

    Article Snippet: Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad).

    Techniques: Expressing, Mouse Assay

    Effects of IL-15 injection on acute expression of pro-oxidative mediators in gastrocnemius muscles of IL-15–KO mice. A and B, Expression of PPARδ, SIRT1, PGC-1α, and PGC-1β mRNA in gastrocnemius muscles of IL-15–KO

    Journal: Endocrinology

    Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

    doi: 10.1210/en.2013-1645

    Figure Lengend Snippet: Effects of IL-15 injection on acute expression of pro-oxidative mediators in gastrocnemius muscles of IL-15–KO mice. A and B, Expression of PPARδ, SIRT1, PGC-1α, and PGC-1β mRNA in gastrocnemius muscles of IL-15–KO

    Article Snippet: Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad).

    Techniques: Injection, Expressing, Mouse Assay, Pyrolysis Gas Chromatography

    Postexhaustive exercise induction of PGC-1α and PGC-1β. A and C, PGC-1α (A) and PGC-1β (C) mRNA expression in gastrocnemius muscles of control and IL-15–KO sedentary mice (SED) or at different time points (immediately

    Journal: Endocrinology

    Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

    doi: 10.1210/en.2013-1645

    Figure Lengend Snippet: Postexhaustive exercise induction of PGC-1α and PGC-1β. A and C, PGC-1α (A) and PGC-1β (C) mRNA expression in gastrocnemius muscles of control and IL-15–KO sedentary mice (SED) or at different time points (immediately

    Article Snippet: Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad).

    Techniques: Pyrolysis Gas Chromatography, Expressing, Mouse Assay

    Postexhaustive exercise induction of PPARδ and SIRT1. A and C, PPARδ (A) and SIRT1(C) gastrocnemius muscle mRNA expression in control and IL-15–KO sedentary (SED) mice at different time points (immediately [IMMED], 30 minutes,

    Journal: Endocrinology

    Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

    doi: 10.1210/en.2013-1645

    Figure Lengend Snippet: Postexhaustive exercise induction of PPARδ and SIRT1. A and C, PPARδ (A) and SIRT1(C) gastrocnemius muscle mRNA expression in control and IL-15–KO sedentary (SED) mice at different time points (immediately [IMMED], 30 minutes,

    Article Snippet: Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad).

    Techniques: Expressing, Mouse Assay

    Postexercise muscle expression of IL-15Rα and circulating IL-15 levels in IL-15Rα–KO mice. A, IL-15Rα mRNA expression in gastrocnemius muscles of sedentary (SED) control and IL-15–KO mice and at intervals after

    Journal: Endocrinology

    Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

    doi: 10.1210/en.2013-1645

    Figure Lengend Snippet: Postexercise muscle expression of IL-15Rα and circulating IL-15 levels in IL-15Rα–KO mice. A, IL-15Rα mRNA expression in gastrocnemius muscles of sedentary (SED) control and IL-15–KO mice and at intervals after

    Article Snippet: Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad).

    Techniques: Expressing, Mouse Assay

    Body composition in control and IL-15–KO mice

    Journal: Endocrinology

    Article Title: IL-15 Is Required for Postexercise Induction of the Pro-Oxidative Mediators PPARδ and SIRT1 in Male Mice

    doi: 10.1210/en.2013-1645

    Figure Lengend Snippet: Body composition in control and IL-15–KO mice

    Article Snippet: Serum was separated and stored frozen at 20°C, and IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (with a sensitivity of 6.6 pg/mL and intra-assay coefficient of variation of 6%) using a Bio-Plex protein array instrument (Bio-Rad).

    Techniques: Mouse Assay

    Nuclear and mitochondrial (Mt) DNA, and markers of Mt activity, in selected tissues of control (CON) and IL-15 Tg (TG) mice. A, Total (predominately nuclear) DNA per milligram tissue; B, Mt DNA per tissue weight; C, CPT1B mRNA expression; D, UCP2 mRNA

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Nuclear and mitochondrial (Mt) DNA, and markers of Mt activity, in selected tissues of control (CON) and IL-15 Tg (TG) mice. A, Total (predominately nuclear) DNA per milligram tissue; B, Mt DNA per tissue weight; C, CPT1B mRNA expression; D, UCP2 mRNA

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Activity Assay, Mouse Assay, Expressing

    Expression of mRNA markers for muscle phenotype in control (CON) and IL-15 Tg (TG) mice in mixed gastrocnemius muscle (GASTROC), EDL, and soleus muscle. A, Troponin I slow; B, Troponin I fast; C, MHC-1, typically expressed in slow/oxidative fibers; D,

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Expression of mRNA markers for muscle phenotype in control (CON) and IL-15 Tg (TG) mice in mixed gastrocnemius muscle (GASTROC), EDL, and soleus muscle. A, Troponin I slow; B, Troponin I fast; C, MHC-1, typically expressed in slow/oxidative fibers; D,

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Expressing, Mouse Assay

    Expression of selected mRNA species involved in fat oxidation in several tissues involved in energy metabolism in untrained control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Expression of selected mRNA species involved in fat oxidation in several tissues involved in energy metabolism in untrained control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Expressing, Mouse Assay

    Phenotypic characteristics of IL-15 Tg and control mice

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Phenotypic characteristics of IL-15 Tg and control mice

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Mouse Assay

    Energy metabolism, activity, and running endurance in control (CON) and IL-15 Tg (TG) mice. A, RER was significantly lower in TG mice, indicating preferential use of fat for energy metabolism. B, Food intake (FI) did not differ between genotypes. C and

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Energy metabolism, activity, and running endurance in control (CON) and IL-15 Tg (TG) mice. A, RER was significantly lower in TG mice, indicating preferential use of fat for energy metabolism. B, Food intake (FI) did not differ between genotypes. C and

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Activity Assay, Mouse Assay

    Differential expression of PGC-1α, PPARγ, and PDK4 in liver and sc fat of control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between genotypes (B, D, and F). A and B,

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Differential expression of PGC-1α, PPARγ, and PDK4 in liver and sc fat of control (CON) and IL-15 Tg (TG) mice. Data for each species are expressed as abundance (A, C, and E) and fold difference between genotypes (B, D, and F). A and B,

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Expressing, Pyrolysis Gas Chromatography, Mouse Assay

    Protein levels of intracellular mediators of oxidative metabolism, analyzed by Western blots, in (CON) and IL-15 Tg (TG) mouse EDL and soleus muscles. A, SIRT1; B, PPARδ; C, PGC-1β; D, PGC-1α. Bars represent means ± sem

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: Protein levels of intracellular mediators of oxidative metabolism, analyzed by Western blots, in (CON) and IL-15 Tg (TG) mouse EDL and soleus muscles. A, SIRT1; B, PPARδ; C, PGC-1β; D, PGC-1α. Bars represent means ± sem

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Western Blot, Pyrolysis Gas Chromatography

    IL-15Rα expression in control (CON) and IL-15 Tg (TG) mice. A, IL-15Rα mRNA expression (n = 6–9 mice per group); B, IL-15Rα protein levels in muscle tissue, determined by Western blot (n = 4 CON and 5 TG). Signal in each

    Journal: Endocrinology

    Article Title: IL-15 Overexpression Promotes Endurance, Oxidative Energy Metabolism, and Muscle PPAR?, SIRT1, PGC-1?, and PGC-1? Expression in Male Mice

    doi: 10.1210/en.2012-1773

    Figure Lengend Snippet: IL-15Rα expression in control (CON) and IL-15 Tg (TG) mice. A, IL-15Rα mRNA expression (n = 6–9 mice per group); B, IL-15Rα protein levels in muscle tissue, determined by Western blot (n = 4 CON and 5 TG). Signal in each

    Article Snippet: IL-15 protein levels were assessed with a Bio-Rad mouse IL-15 kit (sensitivity 6.6 pg/ml; intraassay coefficient of variation 6%) using a BioPlex protein array instrument (Bio-Rad).

    Techniques: Expressing, Mouse Assay, Western Blot

    Proposed interplay between IL-15 and TNF-α signaling in skeletal muscle. Binding of TNF-α to TNF-R1 triggers the extrinsic pathway of apoptosis. Chronic IL-15 stimulation may result in downregulation of TNF-R1 and subsequent prevention of TNF-α-mediated apoptosis (solid line). Actions of IL-15 may be transduced by its cell surface trimeric receptor. IL-15 may also interact with IL-15Rα on the plasma membrane and be presented to adjacent cells (or portions of a muscle fiber) expressing the γ c /IL-2Rβ heterodimer. Soluble IL-15Rα (sIL-15Rα) may be important for secretion, stabilization and activity of IL-15. Additionally, in the presence of an acute apoptotic stimulus, IL-15Rα might counteract DISC assembly and promote the production of anti-apoptotic proteins such as FLIPs and cIAPs via NF-κB activation (dashed lines).

    Journal: Mechanisms of ageing and development

    Article Title: Changes in IL-15 expression and death-receptor apoptotic signaling in rat gastrocnemius muscle with aging and life-long calorie restriction

    doi: 10.1016/j.mad.2008.12.008

    Figure Lengend Snippet: Proposed interplay between IL-15 and TNF-α signaling in skeletal muscle. Binding of TNF-α to TNF-R1 triggers the extrinsic pathway of apoptosis. Chronic IL-15 stimulation may result in downregulation of TNF-R1 and subsequent prevention of TNF-α-mediated apoptosis (solid line). Actions of IL-15 may be transduced by its cell surface trimeric receptor. IL-15 may also interact with IL-15Rα on the plasma membrane and be presented to adjacent cells (or portions of a muscle fiber) expressing the γ c /IL-2Rβ heterodimer. Soluble IL-15Rα (sIL-15Rα) may be important for secretion, stabilization and activity of IL-15. Additionally, in the presence of an acute apoptotic stimulus, IL-15Rα might counteract DISC assembly and promote the production of anti-apoptotic proteins such as FLIPs and cIAPs via NF-κB activation (dashed lines).

    Article Snippet: Concentration of TNF-α and IL-15 was determined by using rat TNF-α and mouse IL-15 assay kits (Bio-Rad), respectively.

    Techniques: Binding Assay, Expressing, Activity Assay, Activation Assay

    Linear regression analysis revealed a negative correlation between TNF-α/IL-15 and muscle weight (MW) expressed as percentage of 8-month-old rats in the two dietary groups (a). A positive correlation was also detected between TNF-α/IL-15 and the apoptotic index (b). Finally, a negative correlation was evidenced between the apoptotic index and MW (c).

    Journal: Mechanisms of ageing and development

    Article Title: Changes in IL-15 expression and death-receptor apoptotic signaling in rat gastrocnemius muscle with aging and life-long calorie restriction

    doi: 10.1016/j.mad.2008.12.008

    Figure Lengend Snippet: Linear regression analysis revealed a negative correlation between TNF-α/IL-15 and muscle weight (MW) expressed as percentage of 8-month-old rats in the two dietary groups (a). A positive correlation was also detected between TNF-α/IL-15 and the apoptotic index (b). Finally, a negative correlation was evidenced between the apoptotic index and MW (c).

    Article Snippet: Concentration of TNF-α and IL-15 was determined by using rat TNF-α and mouse IL-15 assay kits (Bio-Rad), respectively.

    Techniques:

    An age-dependent increase in TNF-α to IL-15 ratio was detected, which was prevented by CR (age: p

    Journal: Mechanisms of ageing and development

    Article Title: Changes in IL-15 expression and death-receptor apoptotic signaling in rat gastrocnemius muscle with aging and life-long calorie restriction

    doi: 10.1016/j.mad.2008.12.008

    Figure Lengend Snippet: An age-dependent increase in TNF-α to IL-15 ratio was detected, which was prevented by CR (age: p

    Article Snippet: Concentration of TNF-α and IL-15 was determined by using rat TNF-α and mouse IL-15 assay kits (Bio-Rad), respectively.

    Techniques: