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Bethyl mouse igg2b elisa quantitation kit
VSV-IFN therapeutic efficacy in mice bearing orthotopic myeloma 5TGM1 cells were implanted IV in syngeneic C57Bl/KalWrij mice. ( A ) Mean serum <t>IgG2b</t> concentration was quantified at 7 day intervals (n=26 mice). Mean serum IgG2b was measured by <t>ELISA</t> and compared using a t-test indicating a significant change in serum IgG2b from day 14 to day 21 post 5TGM1 implantation (*P=0.0019) ( B ) Survival response in myeloma bearing mice following a single IV dose of PBS, or 1×10 8 VSV-mIFNβ or VSV-hIFNβ. Survival was compared by log-rank analysis. VSV-mIFNβ and VSV-hIFNβ treatment significantly prolonged survival compared to PBS (P=0.0008*** and P=0.017* respectively), and survival of mice treated with VSV-mIFNβ is significantly prolonged compared to VSV-hIFNβ treated mice (P=0.021*) ( C ) Myeloma burden in response to IV treatment with PBS, VSV-mIFNβ or VSV-hIFNβ measured by serum IgG2b ELISA.
Mouse Igg2b Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Price from $9.99 to $1999.99
mouse igg2b elisa quantitation kit - by Bioz Stars, 2020-07
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1) Product Images from "Potent systemic therapy of Multiple Myeloma utilizing Oncolytic Vesicular stomatitis virus coding for Interferon-beta"

Article Title: Potent systemic therapy of Multiple Myeloma utilizing Oncolytic Vesicular stomatitis virus coding for Interferon-beta

Journal: Cancer Gene Therapy

doi: 10.1038/cgt.2012.14

VSV-IFN therapeutic efficacy in mice bearing orthotopic myeloma 5TGM1 cells were implanted IV in syngeneic C57Bl/KalWrij mice. ( A ) Mean serum IgG2b concentration was quantified at 7 day intervals (n=26 mice). Mean serum IgG2b was measured by ELISA and compared using a t-test indicating a significant change in serum IgG2b from day 14 to day 21 post 5TGM1 implantation (*P=0.0019) ( B ) Survival response in myeloma bearing mice following a single IV dose of PBS, or 1×10 8 VSV-mIFNβ or VSV-hIFNβ. Survival was compared by log-rank analysis. VSV-mIFNβ and VSV-hIFNβ treatment significantly prolonged survival compared to PBS (P=0.0008*** and P=0.017* respectively), and survival of mice treated with VSV-mIFNβ is significantly prolonged compared to VSV-hIFNβ treated mice (P=0.021*) ( C ) Myeloma burden in response to IV treatment with PBS, VSV-mIFNβ or VSV-hIFNβ measured by serum IgG2b ELISA.
Figure Legend Snippet: VSV-IFN therapeutic efficacy in mice bearing orthotopic myeloma 5TGM1 cells were implanted IV in syngeneic C57Bl/KalWrij mice. ( A ) Mean serum IgG2b concentration was quantified at 7 day intervals (n=26 mice). Mean serum IgG2b was measured by ELISA and compared using a t-test indicating a significant change in serum IgG2b from day 14 to day 21 post 5TGM1 implantation (*P=0.0019) ( B ) Survival response in myeloma bearing mice following a single IV dose of PBS, or 1×10 8 VSV-mIFNβ or VSV-hIFNβ. Survival was compared by log-rank analysis. VSV-mIFNβ and VSV-hIFNβ treatment significantly prolonged survival compared to PBS (P=0.0008*** and P=0.017* respectively), and survival of mice treated with VSV-mIFNβ is significantly prolonged compared to VSV-hIFNβ treated mice (P=0.021*) ( C ) Myeloma burden in response to IV treatment with PBS, VSV-mIFNβ or VSV-hIFNβ measured by serum IgG2b ELISA.

Techniques Used: Mouse Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Gab3-Deficient Mice Exhibit Normal Development and Hematopoiesis and Are Immunocompetent
Article Snippet: .. Serum IgG1 levels from OVA-CFA-treated Gab3 −/− and control mice were measured before and 4 weeks after the first injection by using a mouse IgG1 enzyme-linked immunosorbent assay quantitation kit (Bethyl Laboratories, Montgomery, Tex.). .. Six- to 8-week-old Gab3 −/− and littermate controls were infected with 2 × 105 PFU of the Armstrong strain of lymphocytic choriomeningitis virus (LCMV) by intraperitoneal injection.

Article Title: Potent systemic therapy of Multiple Myeloma utilizing Oncolytic Vesicular stomatitis virus coding for Interferon-beta
Article Snippet: .. Myeloma burden was monitored by measurement of serum IgG2b using Mouse IgG2b ELISA Quantitation kit (Bethyl laboratories, E90–109). .. Mice were monitored for symptoms of myeloma including weight loss, hind limb paralysis or the growth of subscapular plasmacytomas and sacrificed according to the previously described criterion.

Article Title: PARATHYROID HORMONE RECEPTOR MEDIATES THE ANTI-MYELOMA EFFECT OF PROTEASOME INHIBITORS
Article Snippet: .. Serum IgG2b levels were measured as described,[ ] using a Mouse IgG2b ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, TX) according to the manufacturer's instructions. ..

Article Title: Periostin promotes liver steatosis and hypertriglyceridemia through downregulation of PPARα
Article Snippet: .. The concentrations of the obtained mAbs were measured by Mouse IgG ELISA Quantitation kit (Bethyl Laboratories), following the manufacturer’s instructions. .. The kinetic parameters of the mAbs were determined using a Biacore T100 instrument (Biacore AB).

Article Title: Inhibition of Transforming Growth Factor-β Activation Diminishes Tumor Progression and Osteolytic Bone Disease in Mouse Models of Multiple Myeloma
Article Snippet: .. The following enzyme-linked immunosorbent assay (ELISA) kits were used according to the manufacturer's instructions: the Human Kappa ELISA Quantitation Kit and Mouse IgG2b ELISA Quantitation Kit (Bethyl Laboratories, Inc., Montgomery, TX), Quantikine Mouse IL-6 Immunoassay and Human TGF-β1 Quantikine ELISA kit (R & D Systems, Minneapolis, MN), and MouseTrap Assay SB-TR103 (Immunodiagnostic Systems Inc., Fountain Hills, AZ). .. TSP1 was purified from outdated de-identified, pooled human platelets purchased from the American Red Cross (St. Louis, MO).

Article Title: Systemic inflammation in glucocerebrosidase-deficient mice with minimal glucosylceramide storage
Article Snippet: .. IgG levels in plasma were estimated using a mouse IgG ELISA Quantitation kit (Bethyl Laboratories, Inc. Montgomery, Texas, USA). .. As described in Methods, we were first able to produce adult homozygous GbaL444P mice through cross-breeding with mice carrying a knockout glucosylceramide synthase ( Ugcg ) gene , which controls the glucosylceramide synthesis pathway (Figure , a and b).

Article Title: Anti-Glycoprotein H Antibody Impairs the Pathogenicity of Varicella-Zoster Virus in Skin Xenografts in the SCID Mouse Model ▿
Article Snippet: .. The IgG1 MAb 206 in mouse serum was measured using a mouse IgG1 enzyme-linked immunosorbent assay quantitation kit from Bethyl Laboratories, Inc. (Montgomery, TX), following the manufacturer's recommended protocol. ..

Article Title: Negative regulation of inflammatory responses by immunoglobulin A receptor (Fc?RI) inhibits the development of Toll-like receptor-9 signalling-accelerated glomerulonephritis
Article Snippet: .. Serum total IgG levels were measured by ELISA (mouse IgG ELISA Quantitation Kit; Bethyl Laboratories). .. Serum samples containing immune complexes (IC) were precipitated with an equal volume of 3·8% polyethylene glycol 6000 (PEG; Wako, Osaka, Japan) [

Injection:

Article Title: Gab3-Deficient Mice Exhibit Normal Development and Hematopoiesis and Are Immunocompetent
Article Snippet: .. Serum IgG1 levels from OVA-CFA-treated Gab3 −/− and control mice were measured before and 4 weeks after the first injection by using a mouse IgG1 enzyme-linked immunosorbent assay quantitation kit (Bethyl Laboratories, Montgomery, Tex.). .. Six- to 8-week-old Gab3 −/− and littermate controls were infected with 2 × 105 PFU of the Armstrong strain of lymphocytic choriomeningitis virus (LCMV) by intraperitoneal injection.

Quantitation Assay:

Article Title: Gab3-Deficient Mice Exhibit Normal Development and Hematopoiesis and Are Immunocompetent
Article Snippet: .. Serum IgG1 levels from OVA-CFA-treated Gab3 −/− and control mice were measured before and 4 weeks after the first injection by using a mouse IgG1 enzyme-linked immunosorbent assay quantitation kit (Bethyl Laboratories, Montgomery, Tex.). .. Six- to 8-week-old Gab3 −/− and littermate controls were infected with 2 × 105 PFU of the Armstrong strain of lymphocytic choriomeningitis virus (LCMV) by intraperitoneal injection.

Article Title: Potent systemic therapy of Multiple Myeloma utilizing Oncolytic Vesicular stomatitis virus coding for Interferon-beta
Article Snippet: .. Myeloma burden was monitored by measurement of serum IgG2b using Mouse IgG2b ELISA Quantitation kit (Bethyl laboratories, E90–109). .. Mice were monitored for symptoms of myeloma including weight loss, hind limb paralysis or the growth of subscapular plasmacytomas and sacrificed according to the previously described criterion.

Article Title: PARATHYROID HORMONE RECEPTOR MEDIATES THE ANTI-MYELOMA EFFECT OF PROTEASOME INHIBITORS
Article Snippet: .. Serum IgG2b levels were measured as described,[ ] using a Mouse IgG2b ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, TX) according to the manufacturer's instructions. ..

Article Title: Periostin promotes liver steatosis and hypertriglyceridemia through downregulation of PPARα
Article Snippet: .. The concentrations of the obtained mAbs were measured by Mouse IgG ELISA Quantitation kit (Bethyl Laboratories), following the manufacturer’s instructions. .. The kinetic parameters of the mAbs were determined using a Biacore T100 instrument (Biacore AB).

Article Title: Inhibition of Transforming Growth Factor-β Activation Diminishes Tumor Progression and Osteolytic Bone Disease in Mouse Models of Multiple Myeloma
Article Snippet: .. The following enzyme-linked immunosorbent assay (ELISA) kits were used according to the manufacturer's instructions: the Human Kappa ELISA Quantitation Kit and Mouse IgG2b ELISA Quantitation Kit (Bethyl Laboratories, Inc., Montgomery, TX), Quantikine Mouse IL-6 Immunoassay and Human TGF-β1 Quantikine ELISA kit (R & D Systems, Minneapolis, MN), and MouseTrap Assay SB-TR103 (Immunodiagnostic Systems Inc., Fountain Hills, AZ). .. TSP1 was purified from outdated de-identified, pooled human platelets purchased from the American Red Cross (St. Louis, MO).

Article Title: Systemic inflammation in glucocerebrosidase-deficient mice with minimal glucosylceramide storage
Article Snippet: .. IgG levels in plasma were estimated using a mouse IgG ELISA Quantitation kit (Bethyl Laboratories, Inc. Montgomery, Texas, USA). .. As described in Methods, we were first able to produce adult homozygous GbaL444P mice through cross-breeding with mice carrying a knockout glucosylceramide synthase ( Ugcg ) gene , which controls the glucosylceramide synthesis pathway (Figure , a and b).

Article Title: Anti-Glycoprotein H Antibody Impairs the Pathogenicity of Varicella-Zoster Virus in Skin Xenografts in the SCID Mouse Model ▿
Article Snippet: .. The IgG1 MAb 206 in mouse serum was measured using a mouse IgG1 enzyme-linked immunosorbent assay quantitation kit from Bethyl Laboratories, Inc. (Montgomery, TX), following the manufacturer's recommended protocol. ..

Article Title: Negative regulation of inflammatory responses by immunoglobulin A receptor (Fc?RI) inhibits the development of Toll-like receptor-9 signalling-accelerated glomerulonephritis
Article Snippet: .. Serum total IgG levels were measured by ELISA (mouse IgG ELISA Quantitation Kit; Bethyl Laboratories). .. Serum samples containing immune complexes (IC) were precipitated with an equal volume of 3·8% polyethylene glycol 6000 (PEG; Wako, Osaka, Japan) [

Mouse Assay:

Article Title: Gab3-Deficient Mice Exhibit Normal Development and Hematopoiesis and Are Immunocompetent
Article Snippet: .. Serum IgG1 levels from OVA-CFA-treated Gab3 −/− and control mice were measured before and 4 weeks after the first injection by using a mouse IgG1 enzyme-linked immunosorbent assay quantitation kit (Bethyl Laboratories, Montgomery, Tex.). .. Six- to 8-week-old Gab3 −/− and littermate controls were infected with 2 × 105 PFU of the Armstrong strain of lymphocytic choriomeningitis virus (LCMV) by intraperitoneal injection.

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  • 91
    Bethyl mouse igg elisa quantification kit
    PAD2 contributes to plasma cells and <t>IgG</t> levels in TNFα-induced arthritis A. Total serum IgG was quantified for 4–5 month old TNF + PAD2 +/+ and TNF + PAD2 −/− mice by <t>ELISA</t> with average and SEM graphed (n=8, **p
    Mouse Igg Elisa Quantification Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igg elisa quantification kit/product/Bethyl
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mouse igg elisa quantification kit - by Bioz Stars, 2020-07
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    92
    Bethyl igm igg quantification kit
    ( A ) Immunization protocol used for the results presented in B and C, to show the impact of T cell autophagy on long term humoral response. Mice received OVA in complete Freund’s adjuvant (CFA) i.p. at day 0, and at day 10 OVA in incomplete Freund’s adjuvant (IFA). Mice were then injected i.p. at week 12 + 1 day with OVA in IFA. Sera were collected at days 5 and 15 and then at week 12 and week 12 + 5 days. ( B ) Anti-OVA <t>IgM</t> titers as measured by ELISA at days 5 and 15 (n = 10 littermates (LM) and Atg5 f/f dLck-cre mice). ( C ) Total <t>IgG</t> levels measured by ELISA at different time points after the injection (n = 2 B6, n = 6 LM and n = 6 Atg5 f/f dLck-cre mice). ( D ) Longitudinal monitoring of anti-OVA IgG titers as measured by ELISA (n = 6 B6, n = 12 LM and n = 14 Atg5 f/f dLck-cre mice). LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. **p
    Igm Igg Quantification Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm igg quantification kit/product/Bethyl
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    igm igg quantification kit - by Bioz Stars, 2020-07
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    85
    Bethyl mouse igg1 igg2a elisa quantitation kit
    Serum levels of (a) <t>IgG</t> 1 and (b) IgG 2a subtypes in tolerized and nontolerized collagen-induced arthritis (CIA) mice. When examined at 3, 5 and 7 weeks after primary immunization, mice that received oral type II collagen (CII) exhibited higher serum levels of IgG 1 than did nontolerized mice at all three time points, whereas the IgG 2a level was lower in the tolerized group. The data represent average values from 15 mice in each group.
    Mouse Igg1 Igg2a Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igg1 igg2a elisa quantitation kit/product/Bethyl
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mouse igg1 igg2a elisa quantitation kit - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    85
    Bethyl igg1 elisa quantitation kits
    The antigen-specific autoantibodies increased in the obese CIA mice. Ten weeks after the second immunization, sera from the obese CIA and CIA mice ( n = 6 for each group) were collected. The CII-specific total <t>IgG</t> (A) and <t>IgG2a</t> (B) concentrations were determined in the serum samples of the individual mice by <t>ELISA.</t> The data is expressed as the mean ± SD. * P
    Igg1 Elisa Quantitation Kits, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg1 elisa quantitation kits/product/Bethyl
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    igg1 elisa quantitation kits - by Bioz Stars, 2020-07
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    Image Search Results


    PAD2 contributes to plasma cells and IgG levels in TNFα-induced arthritis A. Total serum IgG was quantified for 4–5 month old TNF + PAD2 +/+ and TNF + PAD2 −/− mice by ELISA with average and SEM graphed (n=8, **p

    Journal: Journal of autoimmunity

    Article Title: Peptidylarginine Deiminase 2 is Required for Tumor Necrosis Factor Alpha-Induced Citrullination and Arthritis, but Not Neutrophil Extracellular Trap Formation

    doi: 10.1016/j.jaut.2017.01.006

    Figure Lengend Snippet: PAD2 contributes to plasma cells and IgG levels in TNFα-induced arthritis A. Total serum IgG was quantified for 4–5 month old TNF + PAD2 +/+ and TNF + PAD2 −/− mice by ELISA with average and SEM graphed (n=8, **p

    Article Snippet: Sera were collected by cardiac puncture and subjected to a mouse IgG ELISA quantification kit according to manufacturer’s protocol (Bethyl Laboratories Inc., Montgomery, USA).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    ( A ) Immunization protocol used for the results presented in B and C, to show the impact of T cell autophagy on long term humoral response. Mice received OVA in complete Freund’s adjuvant (CFA) i.p. at day 0, and at day 10 OVA in incomplete Freund’s adjuvant (IFA). Mice were then injected i.p. at week 12 + 1 day with OVA in IFA. Sera were collected at days 5 and 15 and then at week 12 and week 12 + 5 days. ( B ) Anti-OVA IgM titers as measured by ELISA at days 5 and 15 (n = 10 littermates (LM) and Atg5 f/f dLck-cre mice). ( C ) Total IgG levels measured by ELISA at different time points after the injection (n = 2 B6, n = 6 LM and n = 6 Atg5 f/f dLck-cre mice). ( D ) Longitudinal monitoring of anti-OVA IgG titers as measured by ELISA (n = 6 B6, n = 12 LM and n = 14 Atg5 f/f dLck-cre mice). LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. **p

    Journal: Scientific Reports

    Article Title: CD4 T cell autophagy is integral to memory maintenance

    doi: 10.1038/s41598-018-23993-0

    Figure Lengend Snippet: ( A ) Immunization protocol used for the results presented in B and C, to show the impact of T cell autophagy on long term humoral response. Mice received OVA in complete Freund’s adjuvant (CFA) i.p. at day 0, and at day 10 OVA in incomplete Freund’s adjuvant (IFA). Mice were then injected i.p. at week 12 + 1 day with OVA in IFA. Sera were collected at days 5 and 15 and then at week 12 and week 12 + 5 days. ( B ) Anti-OVA IgM titers as measured by ELISA at days 5 and 15 (n = 10 littermates (LM) and Atg5 f/f dLck-cre mice). ( C ) Total IgG levels measured by ELISA at different time points after the injection (n = 2 B6, n = 6 LM and n = 6 Atg5 f/f dLck-cre mice). ( D ) Longitudinal monitoring of anti-OVA IgG titers as measured by ELISA (n = 6 B6, n = 12 LM and n = 14 Atg5 f/f dLck-cre mice). LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. **p

    Article Snippet: Serum levels of total IgG and IgM were determined in immunized mice using the IgM/IgG quantification kit from Bethyl laboratories following manufacturer’s indications (Bethyl, Montgomery, TX, USA, E90-101/E90-131).

    Techniques: Mouse Assay, Immunofluorescence, Injection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    ( A ) Transfer experiment protocol used to determine the intrinsic defect in CD4 + T cell memory in the absence of autophagy. C57BL/6 (B6), littermate (LM) or Atg5 f/f dLck-cre mice received OVA in CFA. B cells were isolated from wild B6 mice and CD4 + T cells isolated from LM or Atg5 f/f dLck-cre mice. B cells and CD4 + T cells were transferred into naive wild type hosts, that were then immunized by OVA in IFA. ( B ) Measurement by ELISA of anti-OVA IgG and IgM in serum, 5 days after immunization of transferred naive hosts with OVA. Each point represents an individual measurement, histograms stand for means and bars represent standard deviation (n = 6 transfers with LM T cells and n = 7 with Atg5 f/f dLck-cre T cells). ( C ) Left: analysis of the proportions of TCRβ + CD4 + cells in the spleen of donor animals before transfer. Each point represents a single measurement. On the right, activation status of purified CD4 + T cells before the injection to host animals. Proportion of naive cells (CD44 lo CD62L hi ) central memory cells (CM, CD44 hi CD62L hi ) and effector memory cells (EM, CD44 hi CD62L lo ) are indicated. ( D ) Left: Dot plots representing the proportion of donor CD4 T cells (CD45.2 + cells among CD4+ cells) at the end of the experiment. Right: summary of the obtained results for the mouse studied. LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. **p

    Journal: Scientific Reports

    Article Title: CD4 T cell autophagy is integral to memory maintenance

    doi: 10.1038/s41598-018-23993-0

    Figure Lengend Snippet: ( A ) Transfer experiment protocol used to determine the intrinsic defect in CD4 + T cell memory in the absence of autophagy. C57BL/6 (B6), littermate (LM) or Atg5 f/f dLck-cre mice received OVA in CFA. B cells were isolated from wild B6 mice and CD4 + T cells isolated from LM or Atg5 f/f dLck-cre mice. B cells and CD4 + T cells were transferred into naive wild type hosts, that were then immunized by OVA in IFA. ( B ) Measurement by ELISA of anti-OVA IgG and IgM in serum, 5 days after immunization of transferred naive hosts with OVA. Each point represents an individual measurement, histograms stand for means and bars represent standard deviation (n = 6 transfers with LM T cells and n = 7 with Atg5 f/f dLck-cre T cells). ( C ) Left: analysis of the proportions of TCRβ + CD4 + cells in the spleen of donor animals before transfer. Each point represents a single measurement. On the right, activation status of purified CD4 + T cells before the injection to host animals. Proportion of naive cells (CD44 lo CD62L hi ) central memory cells (CM, CD44 hi CD62L hi ) and effector memory cells (EM, CD44 hi CD62L lo ) are indicated. ( D ) Left: Dot plots representing the proportion of donor CD4 T cells (CD45.2 + cells among CD4+ cells) at the end of the experiment. Right: summary of the obtained results for the mouse studied. LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. **p

    Article Snippet: Serum levels of total IgG and IgM were determined in immunized mice using the IgM/IgG quantification kit from Bethyl laboratories following manufacturer’s indications (Bethyl, Montgomery, TX, USA, E90-101/E90-131).

    Techniques: Mouse Assay, Isolation, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Standard Deviation, Activation Assay, Purification, Injection, MANN-WHITNEY

    Serum levels of (a) IgG 1 and (b) IgG 2a subtypes in tolerized and nontolerized collagen-induced arthritis (CIA) mice. When examined at 3, 5 and 7 weeks after primary immunization, mice that received oral type II collagen (CII) exhibited higher serum levels of IgG 1 than did nontolerized mice at all three time points, whereas the IgG 2a level was lower in the tolerized group. The data represent average values from 15 mice in each group.

    Journal: Arthritis Research & Therapy

    Article Title: Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen

    doi: 10.1186/ar1169

    Figure Lengend Snippet: Serum levels of (a) IgG 1 and (b) IgG 2a subtypes in tolerized and nontolerized collagen-induced arthritis (CIA) mice. When examined at 3, 5 and 7 weeks after primary immunization, mice that received oral type II collagen (CII) exhibited higher serum levels of IgG 1 than did nontolerized mice at all three time points, whereas the IgG 2a level was lower in the tolerized group. The data represent average values from 15 mice in each group.

    Article Snippet: Analysis of IgG antibody subtypes Blood samples obtained from each mouse at 3, 5 and 7 weeks after primary immunization were used to investigate IgG antibody subtype concentrations using the mouse IgG1 /IgG2a ELISA quantitation kit (Bethyl Lab Co., Montgomery, TX, USA).

    Techniques: Mouse Assay

    The antigen-specific autoantibodies increased in the obese CIA mice. Ten weeks after the second immunization, sera from the obese CIA and CIA mice ( n = 6 for each group) were collected. The CII-specific total IgG (A) and IgG2a (B) concentrations were determined in the serum samples of the individual mice by ELISA. The data is expressed as the mean ± SD. * P

    Journal: Experimental & Molecular Medicine

    Article Title: Obesity aggravates the joint inflammation in a collagen-induced arthritis model through deviation to Th17 differentiation

    doi: 10.3858/emm.2012.44.7.047

    Figure Lengend Snippet: The antigen-specific autoantibodies increased in the obese CIA mice. Ten weeks after the second immunization, sera from the obese CIA and CIA mice ( n = 6 for each group) were collected. The CII-specific total IgG (A) and IgG2a (B) concentrations were determined in the serum samples of the individual mice by ELISA. The data is expressed as the mean ± SD. * P

    Article Snippet: The concentrations of IgG2a and IgG1 were measured using mouse IgG2a and IgG1 ELISA Quantitation Kits (Bethyl Laboratories, Montgomery, TX), respectively.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay