mouse igg1 anti na k atpase α 1  (Santa Cruz Biotechnology)

 
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    Name:
    Na K ATPase α1 Antibody
    Description:
    Anti Na K ATPase α1 Antibody F 2 is a mouse monoclonal IgG1 kappa light chain Na K ATPase α1 antibody provided at 200 µg ml specific for an epitope mapping between amino acids 54 76 near the N terminus of Na K ATPase α1 of human origin Anti Na K ATPase alpha 1 Antibody F 2 is recommended for detection of Na K ATPase α1 of mouse rat human and monkey origin by WB IP IF IHC P and ELISA Anti Na K ATPase alpha 1 Antibody F 2 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems blocking peptide sc 514614 P Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of Na K ATPase α1 F 2 sc 514614
    Catalog Number:
    SC-514614
    Price:
    None
    Category:
    Antibodies Primary Antibodies and ImmunoCruz Conjugates Channel Proteins Sodium Potassium ATPase Protein Antibodies Na K ATPase α1 Antibody F 2
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    Structured Review

    Santa Cruz Biotechnology mouse igg1 anti na k atpase α 1
    Anti Na K ATPase α1 Antibody F 2 is a mouse monoclonal IgG1 kappa light chain Na K ATPase α1 antibody provided at 200 µg ml specific for an epitope mapping between amino acids 54 76 near the N terminus of Na K ATPase α1 of human origin Anti Na K ATPase alpha 1 Antibody F 2 is recommended for detection of Na K ATPase α1 of mouse rat human and monkey origin by WB IP IF IHC P and ELISA Anti Na K ATPase alpha 1 Antibody F 2 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems blocking peptide sc 514614 P Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of Na K ATPase α1 F 2 sc 514614
    https://www.bioz.com/result/mouse igg1 anti na k atpase α 1/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igg1 anti na k atpase α 1 - by Bioz Stars, 2021-09
    93/100 stars

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    Related Articles

    Transgenic Assay:

    Article Title: Amino acid transporter LAT1 in tumor-associated vascular endothelium promotes angiogenesis by regulating cell proliferation and VEGF-A-dependent mTORC1 activation
    Article Snippet: .. Primary antibodies used are as follows: anti-Na+ /K+-ATPase α1 (sc-21712), anti-p70S6K (sc-230), anti-Akt (sc-1618), anti-Src (sc-8056), and anti-PLCγ (sc-7290) from Santa Cruz Biotechnology; anti-phospho-Thr389-p70S6K (9243), anti-S6 ribosomal protein (2217), anti-phospho-Ser235/Ser236-S6 ribosomal protein (4858), anti-eIF2α (eukaryotic initiation factor 2α subunit) (5324), anti-phospho-Ser51-eIF2α (3398), anti-phospho-Thr308-Akt (13038), anti-phospho-Ser473-Akt (4060), anti-Erk1/2 (4696), anti-phospho-Thr202/Tyr204-Erk1/2 (4370), anti-VEGFR2 (9698), anti-phospho-Tyr1175-VEGFR2 (2478), anti-p38 (8690), anti-phospho-Thr180/Tyr182-p38 (4511), anti-phospho-Tyr416-Src (6943), anti-FAK (3285), anti-phospho-Tyr397-FAK (8556), and anti-phospho-Ser1248-PLCγ (8713) from Cell Signaling Technology; anti-LAT1 (KE026) from TransGenic; anti-β-actin (66009–1-Ig) from Proteintech. ..

    Incubation:

    Article Title: DYT-TOR1A Subcellular Proteomics Reveals Selective Vulnerability of the Nuclear Proteome to Cell Stress
    Article Snippet: .. The membrane was then incubated overnight on a shaker at 4 °C in blocking solution amended with the primary antibody [anti-Lamin B1 (Abcam; ab16048; 1:1000), anti-GAPDH (Abcam; ab9485; 1:1000), anti-Na+/K+ - ATPase (Santa Cruz Biotechnology; sc-21712; 1:500), anti-BiP (Santa Cruz Biotechnology; sc-13968; 1:500)]. ..

    Blocking Assay:

    Article Title: DYT-TOR1A Subcellular Proteomics Reveals Selective Vulnerability of the Nuclear Proteome to Cell Stress
    Article Snippet: .. The membrane was then incubated overnight on a shaker at 4 °C in blocking solution amended with the primary antibody [anti-Lamin B1 (Abcam; ab16048; 1:1000), anti-GAPDH (Abcam; ab9485; 1:1000), anti-Na+/K+ - ATPase (Santa Cruz Biotechnology; sc-21712; 1:500), anti-BiP (Santa Cruz Biotechnology; sc-13968; 1:500)]. ..

    other:

    Article Title: Iron induces two distinct Ca2+ signalling cascades in astrocytes
    Article Snippet: Oligo-fectamine, MEMI, fluo-4 AM, sodium-binding benzofuran isophthalate (SBFI) AM, G-agarose bead, TFR antibody, β-actin antibody, GFAP antibody, DMT1 antibody and DMT1 siRNA duplex chains were from Thermo Fisher Scientific (Waltham, MA USA); siRNA duplex chains of TFR, NCX1-3, Cav-3 and Dab2, NCX2 antibody, Na+ /K+ -ATPase alpha1/2 antibody and the secondary antibodies were bought from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Staining:

    Article Title: Canagliflozin increases adenoma burden in female APCMin/+ mice
    Article Snippet: .. The following antibodies were used in in histologic staining: SGLT2: Abcam anti-SGLT2 antibody (ab37296), 1:100 dilution; SGLT1: Abcam anti-SGLT1 antibody (ab14686), 1:100 dilution; Na+ /K+ ATPase: Santa Cruz Biotechnology Na+ /K+ ATPase α1 (C464.6): sc-21712, 1:1500 dilution. ..

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    Santa Cruz Biotechnology mouse anti na k atpase igg
    FATP2 is expressed in proximal tubule membranes. (A) Lysates from crude membrane preparations were immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti–Na + /K + <t>-ATPase</t> antibodies as a loading control (lower panel). (B) HK-2 cells on coverslips were probed with anti-FATP2 antibodies and postfixed in paraformaldehyde (4%, 10 minutes at room temperature), and primary antibody detection was amplified with Alexa Fluor 568–conjugated goat anti-rabbit <t>IgG.</t> (C) Proximal tubule cell lines were surface labeled with biotin, and lysates were precipitated with streptavidin-coated beads, eluted, resolved by SDS-PAGE, and immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti-Glut5 antibodies as a loading control (lower panel).
    Mouse Anti Na K Atpase Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti na k atpase igg/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti na k atpase igg - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology mouse igg1 anti na k atpase α 1
    FATP2 is expressed in proximal tubule membranes. (A) Lysates from crude membrane preparations were immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti–Na + /K + <t>-ATPase</t> antibodies as a loading control (lower panel). (B) HK-2 cells on coverslips were probed with anti-FATP2 antibodies and postfixed in paraformaldehyde (4%, 10 minutes at room temperature), and primary antibody detection was amplified with Alexa Fluor 568–conjugated goat anti-rabbit <t>IgG.</t> (C) Proximal tubule cell lines were surface labeled with biotin, and lysates were precipitated with streptavidin-coated beads, eluted, resolved by SDS-PAGE, and immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti-Glut5 antibodies as a loading control (lower panel).
    Mouse Igg1 Anti Na K Atpase α 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igg1 anti na k atpase α 1/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igg1 anti na k atpase α 1 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    FATP2 is expressed in proximal tubule membranes. (A) Lysates from crude membrane preparations were immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti–Na + /K + -ATPase antibodies as a loading control (lower panel). (B) HK-2 cells on coverslips were probed with anti-FATP2 antibodies and postfixed in paraformaldehyde (4%, 10 minutes at room temperature), and primary antibody detection was amplified with Alexa Fluor 568–conjugated goat anti-rabbit IgG. (C) Proximal tubule cell lines were surface labeled with biotin, and lysates were precipitated with streptavidin-coated beads, eluted, resolved by SDS-PAGE, and immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti-Glut5 antibodies as a loading control (lower panel).

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Kidney Proximal Tubule Lipoapoptosis Is Regulated by Fatty Acid Transporter-2 (FATP2)

    doi: 10.1681/ASN.2017030314

    Figure Lengend Snippet: FATP2 is expressed in proximal tubule membranes. (A) Lysates from crude membrane preparations were immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti–Na + /K + -ATPase antibodies as a loading control (lower panel). (B) HK-2 cells on coverslips were probed with anti-FATP2 antibodies and postfixed in paraformaldehyde (4%, 10 minutes at room temperature), and primary antibody detection was amplified with Alexa Fluor 568–conjugated goat anti-rabbit IgG. (C) Proximal tubule cell lines were surface labeled with biotin, and lysates were precipitated with streptavidin-coated beads, eluted, resolved by SDS-PAGE, and immunoblotted with anti-FATP2 antibodies (upper panel). Membranes were stripped and reprobed with anti-Glut5 antibodies as a loading control (lower panel).

    Article Snippet: Primary antibodies were rabbit anti-FATP2 IgG (GeneTex, Irvine, CA; 1:50, 16 hours, 4°C), rabbit polyclonal FATP2 antisera (1:50, 16 hours, 4°C), rabbit anti-FFA1 IgG (Alamone, Jerusalem, Israel; 1:200, 16 hours, 4°C), mouse anti-CD36 IgA (BD Pharmingen, San Jose, CA; 1:200, 16 hours, 4°C), mouse anti–Na+ /K+ -ATPase IgG (Santa Cruz Biotechnology, Santa Cruz, CA; 1:100, 16 hours, 4°C), or goat anti-Glut5 IgG (Santa Cruz Biotechnology; 1:100, 16 hours, 4°C).

    Techniques: Amplification, Labeling, SDS Page