mouse igg isotype control  (SouthernBiotech)

 
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  • 93
    Name:
    Goat Anti Rat IgG H L Mouse ads HRP
    Description:

    Catalog Number:
    3050-05
    Price:
    None
    Source:
    Pooled antisera from goats hyperimmunized with rat IgG
    Applications:
    Quality tested applications for relevant formats include -ELISA 1-4FLISAFlow Cytometry 5-10Other referenced applications for relevant formats include -Immunohistochemistry-Frozen Sections 11-17Immunohistochemistry-Paraffin Sections 18-20Imuunocytochemistry 21,22Western Blot 23-25Separation 4
    Format:
    HRP (Horseradish Peroxidase)
    Isotype:
    Goat IgG
    Buy from Supplier


    Structured Review

    SouthernBiotech mouse igg isotype control
    Goat Anti Rat IgG H L Mouse ads HRP

    https://www.bioz.com/result/mouse igg isotype control/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igg isotype control - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells"

    Article Title: Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-8-62

    Downregulation of CXCR4 by vesnarinone . (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO ( upper ), 5-FU ( middle ), or CDDP ( lower ), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.
    Figure Legend Snippet: Downregulation of CXCR4 by vesnarinone . (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO ( upper ), 5-FU ( middle ), or CDDP ( lower ), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.

    Techniques Used: Real-time Polymerase Chain Reaction, Incubation, Labeling, Flow Cytometry, Cytometry, Expressing, Staining, Quantitative RT-PCR

    2) Product Images from "Molecular basis of antibody binding to mucin glycopeptides in lung cancer"

    Article Title: Molecular basis of antibody binding to mucin glycopeptides in lung cancer

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2015.3302

    A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).
    Figure Legend Snippet: A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Techniques Used: Flow Cytometry, Cytometry, Staining

    3) Product Images from "Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells"

    Article Title: Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-8-62

    Downregulation of CXCR4 by vesnarinone . (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO ( upper ), 5-FU ( middle ), or CDDP ( lower ), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.
    Figure Legend Snippet: Downregulation of CXCR4 by vesnarinone . (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO ( upper ), 5-FU ( middle ), or CDDP ( lower ), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.

    Techniques Used: Real-time Polymerase Chain Reaction, Incubation, Labeling, Flow Cytometry, Cytometry, Expressing, Staining, Quantitative RT-PCR

    4) Product Images from "Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells"

    Article Title: Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-8-62

    Downregulation of CXCR4 by vesnarinone . (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO ( upper ), 5-FU ( middle ), or CDDP ( lower ), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.
    Figure Legend Snippet: Downregulation of CXCR4 by vesnarinone . (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO ( upper ), 5-FU ( middle ), or CDDP ( lower ), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.

    Techniques Used: Real-time Polymerase Chain Reaction, Incubation, Labeling, Flow Cytometry, Cytometry, Expressing, Staining, Quantitative RT-PCR

    Related Articles

    Produced:

    Article Title: In vitro molecular evolution yields an NEIBM with a potential novel IgG binding property
    Article Snippet: The prokaryotic expression vector pET-32a(+) was purchased from the Novagen Company (Merck KGaA, Darmstadt, Hesse, Germany) and purified using a Ni-NTA column (Amersham Pharmacia Biotech, Piscataway, NJ, USA) in our lab. hIgG, rIgG, bIgG, gIgG and horseradish peroxidase (HRP)-conjugated streptavidin were purchased from Sigma (St. Louis, MO, USA). .. Monoclonal anti-HCV core protein mouse antibodies 10C1, 6A3, 9H7 and 1G7 were produced in our lab and were determined to be from the mIgG1, mIgG2a, mIgG2b and mIgG3 subclasses, respectively, using purified goat anti-mouse IgG subclass-specific reagents (Southern Biotechnology Associates, Birmingham, Ala., USA). .. All antibodies were biotinylated using biotinyl-N-hydroxy-succinimide (Pierce, Rockford, IL, USA).

    Purification:

    Article Title: In vitro molecular evolution yields an NEIBM with a potential novel IgG binding property
    Article Snippet: The prokaryotic expression vector pET-32a(+) was purchased from the Novagen Company (Merck KGaA, Darmstadt, Hesse, Germany) and purified using a Ni-NTA column (Amersham Pharmacia Biotech, Piscataway, NJ, USA) in our lab. hIgG, rIgG, bIgG, gIgG and horseradish peroxidase (HRP)-conjugated streptavidin were purchased from Sigma (St. Louis, MO, USA). .. Monoclonal anti-HCV core protein mouse antibodies 10C1, 6A3, 9H7 and 1G7 were produced in our lab and were determined to be from the mIgG1, mIgG2a, mIgG2b and mIgG3 subclasses, respectively, using purified goat anti-mouse IgG subclass-specific reagents (Southern Biotechnology Associates, Birmingham, Ala., USA). .. All antibodies were biotinylated using biotinyl-N-hydroxy-succinimide (Pierce, Rockford, IL, USA).

    Incubation:

    Article Title: Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells
    Article Snippet: Flow cytometric analysis Logarithmically growing oral cancer cells were trypsinized and fixed in 4% (w/v) paraformaldehyde on ice for 10 min. .. The cells were washed and incubated with anti-human CXCR4 mAb (dilution 1:100, 12G5; BioSource International, Inc. Camarillo, CA) or mouse IgG isotype control (SouthernBiotech, Birmingham, CA) for 30 min at room temperature. .. After being washed twice with PBS, the cells were incubated with PE-labeled goat anti-mouse IgG (Serotec, Sapporo, Japan) for 30 min at room temperature and analyzed with an EPICS flow cytometer (Coulter, San Jose, CA).

    Article Title: Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells
    Article Snippet: .. ACC-M or Hela cells treated with or without chemotherapeutic agents were incubated with mouse IgG isotype control or with anti-CXCR4 monoclonal antibody. .. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein.

    Staining:

    Article Title: Vesnarinone downregulates CXCR4 expression via upregulation of Kr?ppel-like factor 2 in oral cancer cells
    Article Snippet: Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. .. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. ..

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  • 94
    SouthernBiotech mouse igg isotype control
    A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse <t>IgG</t> (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).
    Mouse Igg Isotype Control, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SouthernBiotech igg isotype igg1
    Experiment 2. IFA <t>IgG,</t> <t>IgG1,</t> IgG2a, IgG2b, and IgG3 responses against asexual stage P. yoelii parasites determined in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). Bars represent geometric mean IFA titers, and solid circles represent responses in individual mice.
    Igg Isotype Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SouthernBiotech igg isotype analysis
    Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total <t>IgG</t> and IgM. Data labeled “a” are statistically significantly different [ P
    Igg Isotype Analysis, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg isotype analysis/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg isotype analysis - by Bioz Stars, 2021-05
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    93
    SouthernBiotech human igg
    Anti-hNC16A <t>IgE</t> do not induce BP in neonatal hNC16A mice. Anti-hNC16A IgE recognized recombinant hNC16A by immunoblotting ( a, lane 2), stained the BMZ of hNC16A mouse skin sections by indirect IF ( b ), but failed to induce BP clinically and histologically in neonatal hNC16A mice at 48 hours post i.d. injection ( c ). ( d ) Immunostaining identified only neutrophils (PMN) in anti-hNC16A <t>IgG-injected</t> mouse skin; but no eosinophils were present in the skin of all anti-hNC16A antibody-injected mice. MPO and EPO enzymatic assays revealed significantly increased PMN in the anti-NC16A IgG-injected skin ( e ) but no eosinophil infiltration in the both anti-NC16A IgG- and IgE-injected skin 48 h post injection ( f ). Scale bars = 50 μm for panel b, scale bars = 100 μm for panels c, d. * p
    Human Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Journal: International Journal of Oncology

    Article Title: Molecular basis of antibody binding to mucin glycopeptides in lung cancer

    doi: 10.3892/ijo.2015.3302

    Figure Lengend Snippet: A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Article Snippet: Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Techniques: Flow Cytometry, Cytometry, Staining

    Experiment 2. IFA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses against asexual stage P. yoelii parasites determined in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). Bars represent geometric mean IFA titers, and solid circles represent responses in individual mice.

    Journal: Infection and Immunity

    Article Title: CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine

    doi: 10.1128/IAI.72.2.949-957.2004

    Figure Lengend Snippet: Experiment 2. IFA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses against asexual stage P. yoelii parasites determined in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). Bars represent geometric mean IFA titers, and solid circles represent responses in individual mice.

    Article Snippet: Following the three washes, wells were incubated with anti-mouse IgG or IgG isotype IgG1, IgG2a, IgG2b, or IgG3 antibodies conjugated to fluorescein isothiocyanate (Southern Biotechnology, Birmingham, Ala.).

    Techniques: Immunofluorescence, Mouse Assay

    Experiment 1. ELISA IgG responses in C57BL/6 mice immunized with yMSP1 19 plus CFA (A), yMSP1 19 plus ODN 1826 plus ISA (B), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (C), ODN 1826 plus ISA (D), yMSP1 19 plus ODN 1826 (E), ODN 1826 (F), and ODN 1982 (G). ELISA titers shown are calculated values determined as interpolated titers at an OD of 0.5. Open bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.

    Journal: Infection and Immunity

    Article Title: CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine

    doi: 10.1128/IAI.72.2.949-957.2004

    Figure Lengend Snippet: Experiment 1. ELISA IgG responses in C57BL/6 mice immunized with yMSP1 19 plus CFA (A), yMSP1 19 plus ODN 1826 plus ISA (B), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (C), ODN 1826 plus ISA (D), yMSP1 19 plus ODN 1826 (E), ODN 1826 (F), and ODN 1982 (G). ELISA titers shown are calculated values determined as interpolated titers at an OD of 0.5. Open bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.

    Article Snippet: Following the three washes, wells were incubated with anti-mouse IgG or IgG isotype IgG1, IgG2a, IgG2b, or IgG3 antibodies conjugated to fluorescein isothiocyanate (Southern Biotechnology, Birmingham, Ala.).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    Experiment 2. ELISA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). ELISA titers shown are calculated geometric mean values determined as interpolated titers at an OD of 0.5. Bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.

    Journal: Infection and Immunity

    Article Title: CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine

    doi: 10.1128/IAI.72.2.949-957.2004

    Figure Lengend Snippet: Experiment 2. ELISA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). ELISA titers shown are calculated geometric mean values determined as interpolated titers at an OD of 0.5. Bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.

    Article Snippet: Following the three washes, wells were incubated with anti-mouse IgG or IgG isotype IgG1, IgG2a, IgG2b, or IgG3 antibodies conjugated to fluorescein isothiocyanate (Southern Biotechnology, Birmingham, Ala.).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total IgG and IgM. Data labeled “a” are statistically significantly different [ P

    Journal: mBio

    Article Title: Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of Mycobacterium tuberculosis

    doi: 10.1128/mBio.01921-14

    Figure Lengend Snippet: Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total IgG and IgM. Data labeled “a” are statistically significantly different [ P

    Article Snippet: For IgG isotype analysis, IgG1, IgG2c, IgG2b, and IgG3 AP-conjugated secondary antibodies (1:1,000) were used (Southern Biotechnologies).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Injection, Labeling

    Anti-hNC16A IgE do not induce BP in neonatal hNC16A mice. Anti-hNC16A IgE recognized recombinant hNC16A by immunoblotting ( a, lane 2), stained the BMZ of hNC16A mouse skin sections by indirect IF ( b ), but failed to induce BP clinically and histologically in neonatal hNC16A mice at 48 hours post i.d. injection ( c ). ( d ) Immunostaining identified only neutrophils (PMN) in anti-hNC16A IgG-injected mouse skin; but no eosinophils were present in the skin of all anti-hNC16A antibody-injected mice. MPO and EPO enzymatic assays revealed significantly increased PMN in the anti-NC16A IgG-injected skin ( e ) but no eosinophil infiltration in the both anti-NC16A IgG- and IgE-injected skin 48 h post injection ( f ). Scale bars = 50 μm for panel b, scale bars = 100 μm for panels c, d. * p

    Journal: The Journal of investigative dermatology

    Article Title: Eosinophils mediate tissue injury in autoimmune skin disease bullous pemphigoid

    doi: 10.1016/j.jid.2017.11.031

    Figure Lengend Snippet: Anti-hNC16A IgE do not induce BP in neonatal hNC16A mice. Anti-hNC16A IgE recognized recombinant hNC16A by immunoblotting ( a, lane 2), stained the BMZ of hNC16A mouse skin sections by indirect IF ( b ), but failed to induce BP clinically and histologically in neonatal hNC16A mice at 48 hours post i.d. injection ( c ). ( d ) Immunostaining identified only neutrophils (PMN) in anti-hNC16A IgG-injected mouse skin; but no eosinophils were present in the skin of all anti-hNC16A antibody-injected mice. MPO and EPO enzymatic assays revealed significantly increased PMN in the anti-NC16A IgG-injected skin ( e ) but no eosinophil infiltration in the both anti-NC16A IgG- and IgE-injected skin 48 h post injection ( f ). Scale bars = 50 μm for panel b, scale bars = 100 μm for panels c, d. * p

    Article Snippet: The concentrations of purified IgG and IgE were quantified by human IgG- and IgE-specific ELISA (Southern Biotechnology).

    Techniques: Mouse Assay, Recombinant, Staining, Injection, Immunostaining

    Anti-hNC16A IgE induce Eos infiltration but do not induce BP in adult hNC16A mice. Adult (8 week old) hNC16A mice were injected in the ear pinna and neonatal (24-36 h old) hNC16A mice were injected i.d. at dorsal back with anti-hNC16A IgE (100 ng/site) or anti-hNC16A IgG (100 μg/g body weight). Anti-hNC16A IgG induced infiltration of neutrophils ( a ) but not eosinophils ( c ) in both neonatal and adult mice and also triggered derma-epidermal separation in the IgG-injected ear ( e ). Anti-hNC16A IgE induced no neutrophil nor eosinophil infiltration in neonatal mice but increased neutrophil and eosinophil infiltration in adult mice at 24 and 48 time points ( b , d ). However, anti-hNC16A IgE-injected ear skin showed no derma-epidermal separation ( f ). Scale bars = 100 μm. 2-Way ANOVA, * p

    Journal: The Journal of investigative dermatology

    Article Title: Eosinophils mediate tissue injury in autoimmune skin disease bullous pemphigoid

    doi: 10.1016/j.jid.2017.11.031

    Figure Lengend Snippet: Anti-hNC16A IgE induce Eos infiltration but do not induce BP in adult hNC16A mice. Adult (8 week old) hNC16A mice were injected in the ear pinna and neonatal (24-36 h old) hNC16A mice were injected i.d. at dorsal back with anti-hNC16A IgE (100 ng/site) or anti-hNC16A IgG (100 μg/g body weight). Anti-hNC16A IgG induced infiltration of neutrophils ( a ) but not eosinophils ( c ) in both neonatal and adult mice and also triggered derma-epidermal separation in the IgG-injected ear ( e ). Anti-hNC16A IgE induced no neutrophil nor eosinophil infiltration in neonatal mice but increased neutrophil and eosinophil infiltration in adult mice at 24 and 48 time points ( b , d ). However, anti-hNC16A IgE-injected ear skin showed no derma-epidermal separation ( f ). Scale bars = 100 μm. 2-Way ANOVA, * p

    Article Snippet: The concentrations of purified IgG and IgE were quantified by human IgG- and IgE-specific ELISA (Southern Biotechnology).

    Techniques: Mouse Assay, Injection