mef cells  (ATCC)


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    ATCC mef cells
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts mefs  (ATCC)


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    ATCC mouse embryonic fibroblasts mefs
    Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic <t>murine</t> <t>embryonic</t> fibroblasts. Co-culture of PBMC with non-engineered <t>MEFs</t> was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.
    Mouse Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts mefs - by Bioz Stars, 2023-01
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    1) Product Images from "Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy"

    Article Title: Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.102013

    Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic murine embryonic fibroblasts. Co-culture of PBMC with non-engineered MEFs was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.
    Figure Legend Snippet: Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic murine embryonic fibroblasts. Co-culture of PBMC with non-engineered MEFs was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.

    Techniques Used: Co-Culture Assay, Transgenic Assay, Negative Control, Expressing


    Figure Legend Snippet:

    Techniques Used: Staining, Recombinant, Modification, Software, Cell Culture, Microscopy, Flow Cytometry, Fluorescence

    mouse embryonic fibroblasts mef  (ATCC)


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    ATCC mouse embryonic fibroblasts mef
    Mouse Embryonic Fibroblasts Mef, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts mefs  (ATCC)


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    ATCC mouse embryonic fibroblasts mefs
    a , Left panel: sequence comparison of hAtg3 C-terminal residues 262 to 277 with the analogous region in selected organisms. Right panel: helical wheel plot for hAtg3 residues 265 to 277. b , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its mutants (n = 3). CL is the control without liposomes. Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. c , Left panel: sequence comparison of hAtg3 C-terminal residues 291 to 300 with the analogous region in selected organisms. Right panel: helical wheel plot for hAtg3 residues 291 to 300. d , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its mutants (n = 3). CL is the control without liposomes. Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. e , Representative immunoblot (n = 4 <t>blots).</t> <t>Atg3</t> knockout (Atg3 -/- ) mouse embryonic fibroblasts <t>(MEFs)</t> stably expressing mCherry EV (empty vector), mCherry-hAtg3 WT (wildtype), mCherry-hAtg3 F296L , or mCherry-hAtg3 F296S mutant were cultured in complete media (CM) with 100 nM bafilomycin A1 (BafA1 to block LC3-II degradation) for 3 hrs and subjected to immunoblotting with the indicated antibodies. f , Quantitative analysis of the relative LC3B–II level (n = 4 blots) in in vivo LC3B lipidation experiments. Statistical analysis was performed using one-way ANOVA test followed by Turkey’s multiple comparisons test. Data are presented as mean ± SD. P values: **** P < 0.0001; ns, not significant.
    Mouse Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Translating Membrane Geometry into Protein Function: Multifaceted Membrane Interactions of Human Atg3 Promote LC3-Phosphatidylethanolamine Conjugation during Autophagy"

    Article Title: Translating Membrane Geometry into Protein Function: Multifaceted Membrane Interactions of Human Atg3 Promote LC3-Phosphatidylethanolamine Conjugation during Autophagy

    Journal: bioRxiv

    doi: 10.1101/2022.12.23.521840

    a , Left panel: sequence comparison of hAtg3 C-terminal residues 262 to 277 with the analogous region in selected organisms. Right panel: helical wheel plot for hAtg3 residues 265 to 277. b , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its mutants (n = 3). CL is the control without liposomes. Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. c , Left panel: sequence comparison of hAtg3 C-terminal residues 291 to 300 with the analogous region in selected organisms. Right panel: helical wheel plot for hAtg3 residues 291 to 300. d , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its mutants (n = 3). CL is the control without liposomes. Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. e , Representative immunoblot (n = 4 blots). Atg3 knockout (Atg3 -/- ) mouse embryonic fibroblasts (MEFs) stably expressing mCherry EV (empty vector), mCherry-hAtg3 WT (wildtype), mCherry-hAtg3 F296L , or mCherry-hAtg3 F296S mutant were cultured in complete media (CM) with 100 nM bafilomycin A1 (BafA1 to block LC3-II degradation) for 3 hrs and subjected to immunoblotting with the indicated antibodies. f , Quantitative analysis of the relative LC3B–II level (n = 4 blots) in in vivo LC3B lipidation experiments. Statistical analysis was performed using one-way ANOVA test followed by Turkey’s multiple comparisons test. Data are presented as mean ± SD. P values: **** P < 0.0001; ns, not significant.
    Figure Legend Snippet: a , Left panel: sequence comparison of hAtg3 C-terminal residues 262 to 277 with the analogous region in selected organisms. Right panel: helical wheel plot for hAtg3 residues 265 to 277. b , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its mutants (n = 3). CL is the control without liposomes. Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. c , Left panel: sequence comparison of hAtg3 C-terminal residues 291 to 300 with the analogous region in selected organisms. Right panel: helical wheel plot for hAtg3 residues 291 to 300. d , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its mutants (n = 3). CL is the control without liposomes. Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. e , Representative immunoblot (n = 4 blots). Atg3 knockout (Atg3 -/- ) mouse embryonic fibroblasts (MEFs) stably expressing mCherry EV (empty vector), mCherry-hAtg3 WT (wildtype), mCherry-hAtg3 F296L , or mCherry-hAtg3 F296S mutant were cultured in complete media (CM) with 100 nM bafilomycin A1 (BafA1 to block LC3-II degradation) for 3 hrs and subjected to immunoblotting with the indicated antibodies. f , Quantitative analysis of the relative LC3B–II level (n = 4 blots) in in vivo LC3B lipidation experiments. Statistical analysis was performed using one-way ANOVA test followed by Turkey’s multiple comparisons test. Data are presented as mean ± SD. P values: **** P < 0.0001; ns, not significant.

    Techniques Used: Sequencing, SDS Page, Western Blot, Knock-Out, Stable Transfection, Expressing, Plasmid Preparation, Mutagenesis, Cell Culture, Blocking Assay, In Vivo

    a , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its H266L and H266F mutants under different pHs (n = 3). CL is the control without liposomes. Asterisk indicates the degradation of LC3B in the presence of ATP. b , Plot of LC3B-PE formation at 1.5 hr for hAtg3 and its H266L and H266F mutants against pHs. Data are presented as mean ± SD. Quantification of conjugation reactions were obtained from three separate measurements (n = 3). c , Representative immunoblot (n = 5 blots). Atg3 knockout (Atg3 -/- ) mouse embryonic fibroblasts (MEFs) stably expressing mCherry EV (empty vector), mCherry-hAtg3 WT (wildtype), mCherry-hAtg3 H266F , mCherry-hAtg3 H266K , or mCherry-hAtg3 H266L mutant were cultured in complete media (CM) with 100 nM bafilomycin A1 (BafA1 to block LC3-II degradation) for 3 hrs and subjected to immunoblotting with the indicated antibodies. d , Quantitative analysis of the relative LC3B-II level (n = 5 blots) in in vivo LC3B lipidation experiments. Statistical analysis was performed using one-way ANOVA test followed by Turkey’s multiple comparisons test. Data are presented as mean ± SD. P values: **** P < 0.0001; ns, not significant.
    Figure Legend Snippet: a , Representative SDS-PAGE gel images of time dependent formation of LC3B-PE for hAtg3 and its H266L and H266F mutants under different pHs (n = 3). CL is the control without liposomes. Asterisk indicates the degradation of LC3B in the presence of ATP. b , Plot of LC3B-PE formation at 1.5 hr for hAtg3 and its H266L and H266F mutants against pHs. Data are presented as mean ± SD. Quantification of conjugation reactions were obtained from three separate measurements (n = 3). c , Representative immunoblot (n = 5 blots). Atg3 knockout (Atg3 -/- ) mouse embryonic fibroblasts (MEFs) stably expressing mCherry EV (empty vector), mCherry-hAtg3 WT (wildtype), mCherry-hAtg3 H266F , mCherry-hAtg3 H266K , or mCherry-hAtg3 H266L mutant were cultured in complete media (CM) with 100 nM bafilomycin A1 (BafA1 to block LC3-II degradation) for 3 hrs and subjected to immunoblotting with the indicated antibodies. d , Quantitative analysis of the relative LC3B-II level (n = 5 blots) in in vivo LC3B lipidation experiments. Statistical analysis was performed using one-way ANOVA test followed by Turkey’s multiple comparisons test. Data are presented as mean ± SD. P values: **** P < 0.0001; ns, not significant.

    Techniques Used: SDS Page, Conjugation Assay, Western Blot, Knock-Out, Stable Transfection, Expressing, Plasmid Preparation, Mutagenesis, Cell Culture, Blocking Assay, In Vivo

    mouse embryonic fibroblast mef cells  (ATCC)


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    ATCC mouse embryonic fibroblast mef cells
    Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts mef  (ATCC)


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    ATCC mouse embryonic fibroblasts mef
    <t>NFAT5-deficient</t> <t>MEF</t> cotransfected with the indicated constructs and the ORE-Luc reporter were cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (510 mOsm/kg) during 20 hours. The activity of each construct is represented as relative to that of DBD5 (transcriptionally inactive) in cells cultured in isotonic medium (arbitrary value of 1). Results are the mean±SD of three independent experiments.
    Mouse Embryonic Fibroblasts Mef, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Exclusion of NFAT5 from Mitotic Chromatin Resets Its Nucleo-Cytoplasmic Distribution in Interphase"

    Article Title: Exclusion of NFAT5 from Mitotic Chromatin Resets Its Nucleo-Cytoplasmic Distribution in Interphase

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007036

    NFAT5-deficient MEF cotransfected with the indicated constructs and the ORE-Luc reporter were cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (510 mOsm/kg) during 20 hours. The activity of each construct is represented as relative to that of DBD5 (transcriptionally inactive) in cells cultured in isotonic medium (arbitrary value of 1). Results are the mean±SD of three independent experiments.
    Figure Legend Snippet: NFAT5-deficient MEF cotransfected with the indicated constructs and the ORE-Luc reporter were cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (510 mOsm/kg) during 20 hours. The activity of each construct is represented as relative to that of DBD5 (transcriptionally inactive) in cells cultured in isotonic medium (arbitrary value of 1). Results are the mean±SD of three independent experiments.

    Techniques Used: Construct, Cell Culture, Activity Assay

    NFAT5-deficient MEF were cotransfected with a constant amount of the ORE-Luc reporter plus different concentrations of expression vectors (1.5 to 6 µg of DNA/9.4 cm 2 -well) encoding wild-type NFAT5a (FL5), a constitutively nuclear mutant (FL5 AED ) or an inactive DNA binding mutant (FL5 DB1 ), and then were cultured in isotonic medium (290 mOsm/kg) or exposed to hypertonic conditions (510 mOsm/kg) during 20 hours. The activity of each construct is represented as relative to that of 1.5 µg of FL5 in isotonic medium (arbitrary value of 1). Results are the mean±SEM of four independent experiments (* p<0.05).
    Figure Legend Snippet: NFAT5-deficient MEF were cotransfected with a constant amount of the ORE-Luc reporter plus different concentrations of expression vectors (1.5 to 6 µg of DNA/9.4 cm 2 -well) encoding wild-type NFAT5a (FL5), a constitutively nuclear mutant (FL5 AED ) or an inactive DNA binding mutant (FL5 DB1 ), and then were cultured in isotonic medium (290 mOsm/kg) or exposed to hypertonic conditions (510 mOsm/kg) during 20 hours. The activity of each construct is represented as relative to that of 1.5 µg of FL5 in isotonic medium (arbitrary value of 1). Results are the mean±SEM of four independent experiments (* p<0.05).

    Techniques Used: Expressing, Mutagenesis, Binding Assay, Cell Culture, Activity Assay, Construct

    mouse embryonic fibroblasts mefs  (ATCC)


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    ATCC mouse embryonic fibroblasts mefs
    Mouse Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblast mef cell lines  (ATCC)


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    ATCC mouse embryonic fibroblast mef cell lines
    A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated <t>MEF-1</t> cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.
    Mouse Embryonic Fibroblast Mef Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Telomerase Variant A279T Induces Telomere Dysfunction and Inhibits Non-Canonical Telomerase Activity in Esophageal Carcinomas"

    Article Title: Telomerase Variant A279T Induces Telomere Dysfunction and Inhibits Non-Canonical Telomerase Activity in Esophageal Carcinomas

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0101010

    A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated MEF-1 cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.
    Figure Legend Snippet: A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated MEF-1 cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.

    Techniques Used: Expressing, Transfection

    balb c murine embryonic fibroblasts mefs  (ATCC)


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    ATCC balb c murine embryonic fibroblasts mefs
    Balb C Murine Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mef cells
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mouse embryonic fibroblasts mefs
    Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic <t>murine</t> <t>embryonic</t> fibroblasts. Co-culture of PBMC with non-engineered <t>MEFs</t> was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.
    Mouse Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mouse embryonic fibroblasts mef
    Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic <t>murine</t> <t>embryonic</t> fibroblasts. Co-culture of PBMC with non-engineered <t>MEFs</t> was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.
    Mouse Embryonic Fibroblasts Mef, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse embryonic fibroblasts mef/product/ATCC
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    ATCC mouse embryonic fibroblast mef cells
    Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic <t>murine</t> <t>embryonic</t> fibroblasts. Co-culture of PBMC with non-engineered <t>MEFs</t> was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.
    Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse embryonic fibroblast mef cells/product/ATCC
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    ATCC mouse embryonic fibroblast mef cell lines
    A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated <t>MEF-1</t> cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.
    Mouse Embryonic Fibroblast Mef Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC balb c murine embryonic fibroblasts mefs
    A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated <t>MEF-1</t> cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.
    Balb C Murine Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic murine embryonic fibroblasts. Co-culture of PBMC with non-engineered MEFs was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.

    Journal: STAR Protocols

    Article Title: Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy

    doi: 10.1016/j.xpro.2022.102013

    Figure Lengend Snippet: Exemplary gating strategies for primary human T cells and murine T cell line (A) Trogocytosis by activated primary human CD4 + T cells after 12 h co-culture of PBMC with CD80-mScarlet transgenic murine embryonic fibroblasts. Co-culture of PBMC with non-engineered MEFs was used as negative control. (B) Trogocytosis of CTLA4-transgenic murine 58 αβ T cells after 2 h co-culture with CD80-TagRFP transgenic CHO cells. 58 αβ T cells lacking expression of CTLA4 and CD28 were used as negative control.

    Article Snippet: Mouse embryonic fibroblasts (MEFs) , ATCC , CRL-2907.

    Techniques: Co-Culture Assay, Transgenic Assay, Negative Control, Expressing

    Journal: STAR Protocols

    Article Title: Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy

    doi: 10.1016/j.xpro.2022.102013

    Figure Lengend Snippet:

    Article Snippet: Mouse embryonic fibroblasts (MEFs) , ATCC , CRL-2907.

    Techniques: Staining, Recombinant, Modification, Software, Cell Culture, Microscopy, Flow Cytometry, Fluorescence

    A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated MEF-1 cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.

    Journal: PLoS ONE

    Article Title: Telomerase Variant A279T Induces Telomere Dysfunction and Inhibits Non-Canonical Telomerase Activity in Esophageal Carcinomas

    doi: 10.1371/journal.pone.0101010

    Figure Lengend Snippet: A. SKY assay demonstrating that A279T-induces genomic instability in Zeocin™-treated MEF-1 cells. Upper panel: translocations, dicentric, and rearranged chromosomes are present in cells expressing A279T compared to wtTERT. Lower left panel: a multi-centric chromosome observed in cells harboring A279T. Lower right panel: a ring chromosome is formed and every chromosome is rearranged in cells transfected with A279T. See text for additional details. B. Upper panel: representative results of SKY analysis Li Fraumeni fibroblasts constitutively expressing wtTERT or A279T-TERT. Lower panel: close-up of chromosomes 1 and 16. C. Summary of results of two independent experiments demonstrating that A279T expression increases genomic instability in Li Fraumeni cells.

    Article Snippet: HCT116, HeLa, and mouse embryonic fibroblast (MEF) cell lines were obtained from American Type Culture Collection (Manassas, VA).

    Techniques: Expressing, Transfection