Structured Review

Stratagene mouse dmrt7 cdna fragment
Expression of <t>Dmrt7</t> mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. <t>cDNA</t> from each organ was amplified with primers specific for Dmrt7 (top row) and β- actin (bottom row). (B) Dmrt7 mRNA expression during the first round of spermatogenesis. cDNAs obtained from testis at the indicated days after birth were amplified as in (A). (C) DMRT7 protein expression. Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). (D) DMRT7 subcellular localization to XY body. Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). SUMO-1 is localized to the XY body. Right-most panel shows merge of other two panels. Inserts show higher magnification of pachytene spermatocytes with XY bodies.
Mouse Dmrt7 Cdna Fragment, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse dmrt7 cdna fragment/product/Stratagene
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse dmrt7 cdna fragment - by Bioz Stars, 2020-08
85/100 stars

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129/svj strain

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1) Product Images from "A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis"

Article Title: A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis

Journal: PLoS Genetics

doi: 10.1371/journal.pgen.0030062

Expression of Dmrt7 mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. cDNA from each organ was amplified with primers specific for Dmrt7 (top row) and β- actin (bottom row). (B) Dmrt7 mRNA expression during the first round of spermatogenesis. cDNAs obtained from testis at the indicated days after birth were amplified as in (A). (C) DMRT7 protein expression. Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). (D) DMRT7 subcellular localization to XY body. Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). SUMO-1 is localized to the XY body. Right-most panel shows merge of other two panels. Inserts show higher magnification of pachytene spermatocytes with XY bodies.
Figure Legend Snippet: Expression of Dmrt7 mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. cDNA from each organ was amplified with primers specific for Dmrt7 (top row) and β- actin (bottom row). (B) Dmrt7 mRNA expression during the first round of spermatogenesis. cDNAs obtained from testis at the indicated days after birth were amplified as in (A). (C) DMRT7 protein expression. Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). (D) DMRT7 subcellular localization to XY body. Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). SUMO-1 is localized to the XY body. Right-most panel shows merge of other two panels. Inserts show higher magnification of pachytene spermatocytes with XY bodies.

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Immunofluorescence, Staining

Related Articles

Sequencing:

Article Title: A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis
Article Snippet: .. A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com ), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence. .. The targeting vector pDZ169 (diagrammed in ) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [ ].

Clone Assay:

Article Title: A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis
Article Snippet: .. A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com ), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence. .. The targeting vector pDZ169 (diagrammed in ) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [ ].

BAC Assay:

Article Title: A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis
Article Snippet: .. A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com ), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence. .. The targeting vector pDZ169 (diagrammed in ) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [ ].

Isolation:

Article Title: A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis
Article Snippet: .. A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com ), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence. .. The targeting vector pDZ169 (diagrammed in ) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [ ].

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    Stratagene mouse dmrt7 cdna fragment
    Expression of <t>Dmrt7</t> mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. <t>cDNA</t> from each organ was amplified with primers specific for Dmrt7 (top row) and β- actin (bottom row). (B) Dmrt7 mRNA expression during the first round of spermatogenesis. cDNAs obtained from testis at the indicated days after birth were amplified as in (A). (C) DMRT7 protein expression. Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). (D) DMRT7 subcellular localization to XY body. Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). SUMO-1 is localized to the XY body. Right-most panel shows merge of other two panels. Inserts show higher magnification of pachytene spermatocytes with XY bodies.
    Mouse Dmrt7 Cdna Fragment, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse dmrt7 cdna fragment/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse dmrt7 cdna fragment - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Expression of Dmrt7 mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. cDNA from each organ was amplified with primers specific for Dmrt7 (top row) and β- actin (bottom row). (B) Dmrt7 mRNA expression during the first round of spermatogenesis. cDNAs obtained from testis at the indicated days after birth were amplified as in (A). (C) DMRT7 protein expression. Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). (D) DMRT7 subcellular localization to XY body. Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). SUMO-1 is localized to the XY body. Right-most panel shows merge of other two panels. Inserts show higher magnification of pachytene spermatocytes with XY bodies.

    Journal: PLoS Genetics

    Article Title: A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis

    doi: 10.1371/journal.pgen.0030062

    Figure Lengend Snippet: Expression of Dmrt7 mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. cDNA from each organ was amplified with primers specific for Dmrt7 (top row) and β- actin (bottom row). (B) Dmrt7 mRNA expression during the first round of spermatogenesis. cDNAs obtained from testis at the indicated days after birth were amplified as in (A). (C) DMRT7 protein expression. Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). (D) DMRT7 subcellular localization to XY body. Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). SUMO-1 is localized to the XY body. Right-most panel shows merge of other two panels. Inserts show higher magnification of pachytene spermatocytes with XY bodies.

    Article Snippet: A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com ), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Immunofluorescence, Staining