balb c mouse crc cell line ct26  (ATCC)

Journal: Frontiers in Oncology

Article Title: DSP-0509, a TLR7 agonist, exerted synergistic anti-tumor immunity combined with various immune therapies through modulating diverse immune cells in cancer microenvironment

doi: 10.3389/fonc.2024.1410373

Figure Lengend Snippet: scRNA-seq of TILs revealed that DSP-0509 induced the infiltration of a wide variety of immune cells in tumors. (A, B) Uniform manifold approximation and projection (UMAP) representation of cell populations found by the clustering of CD45 + cells derived from CT26 bearing mouse from all groups (A) or each group (B) . Tumor infiltrated CD45 + cells were isolated from the CT26-bearing mouse 4 days after administration of vehicle or DSP-0509 5 mg/kg intravenously. CD45 + cells in each group were pooled from 2 mice. Isolated CD45 + cells were used for scRNA-seq analysis. (C) The quantification of the cell fraction of (B) . *Adjusted P < 0.05 in the Fisher test.

Article Snippet: In brief, Tregs (CD4 + CD25 + CD45RB low ) and CD4 + T cells (CD4 + CD25 - CD45RB high ) from the spleens of CT26 tumor-bearing mice were separated by a MoFlo Astrios cell sorter (Beckman Coulter, Inc.) after pre-sorting with mouse CD90.2 MACS beads (Miltenyi Biotec B.V. & Co. KG).

Techniques: Derivative Assay, Isolation

Journal: Frontiers in Oncology

Article Title: DSP-0509, a TLR7 agonist, exerted synergistic anti-tumor immunity combined with various immune therapies through modulating diverse immune cells in cancer microenvironment

doi: 10.3389/fonc.2024.1410373

Figure Lengend Snippet: DSP-0509 modulated the activities of myeloid cells including macrophages and MDSCs. (A) Representative dot plot of the tumor associated macrophage population before and after MACS microbeads isolation. Tumor associated macrophages were defined as the CD11b + F4/80 + fraction. (B) Macrophages were isolated from CT26-Her2 tumors in mice treated with or without DSP-0509 5 mg/kg i.v. once weekly. The tumors were collected at day 11, and F4/80 + macrophages were isolated. CT26-Her2 cells were co-cultured with macrophages for 5 h at the indicated ratio. *P < 0.001 for the Student t test. Values are the mean ± S.E.M. of each group, n = 3/group. (C) mMDSCs were isolated from Renca tumor-bearing mice by using a cell sorter as the CD11b + Gr-1 mid cell fraction. Isolated CD8 + or CD4 + T cells were co-cultured with mMDSCs (1:1) for 3 days. Values are the average ± S.E.M. n = 3/group. *P < 0.001 in the parametric Dunnett test vs the MDSC group. n.s., not significant.

Article Snippet: In brief, Tregs (CD4 + CD25 + CD45RB low ) and CD4 + T cells (CD4 + CD25 - CD45RB high ) from the spleens of CT26 tumor-bearing mice were separated by a MoFlo Astrios cell sorter (Beckman Coulter, Inc.) after pre-sorting with mouse CD90.2 MACS beads (Miltenyi Biotec B.V. & Co. KG).

Techniques: Isolation, Cell Culture

Journal: Frontiers in Oncology

Article Title: DSP-0509, a TLR7 agonist, exerted synergistic anti-tumor immunity combined with various immune therapies through modulating diverse immune cells in cancer microenvironment

doi: 10.3389/fonc.2024.1410373

Figure Lengend Snippet: DSP-0509 combined with anti-PD-1 modulated the tumor microenvironment and increased the size of the effector-like CD8 + T cell cluster population. (A) Tumor growth in CT26 model. DSP-0509 5 mg/kg i.v. was administered once weekly and anti-PD-1 antibody 200 μg was administered biweekly. *P < 0.05 for the Dunnett test. n = 6/group and the values are the mean and S.E.M. (B) UMAP representation of cell clusters in a CD45 + cell population derived from CT26 tumors. (C) Frequencies of identified immune cell populations in TILs. ★ Adjusted P < 0.05 in the Fisher test. R, responder; NR, non-responder for combo therapy. (D) UMAP representation of cell clusters in a CD8 + T cell population. (E) Expression profiles of marker genes for T cell subtypes in identified CD8 + T cell clusters. (F) Frequencies of CD8 + T cell clusters in each treatment group. (G) Comparison of gene expression between cells in cluster 2 and cells in other clusters shown as a volcano plot.

Article Snippet: In brief, Tregs (CD4 + CD25 + CD45RB low ) and CD4 + T cells (CD4 + CD25 - CD45RB high ) from the spleens of CT26 tumor-bearing mice were separated by a MoFlo Astrios cell sorter (Beckman Coulter, Inc.) after pre-sorting with mouse CD90.2 MACS beads (Miltenyi Biotec B.V. & Co. KG).

Techniques: Derivative Assay, Expressing, Marker, Comparison

Journal: Frontiers in Oncology

Article Title: DSP-0509, a TLR7 agonist, exerted synergistic anti-tumor immunity combined with various immune therapies through modulating diverse immune cells in cancer microenvironment

doi: 10.3389/fonc.2024.1410373

Figure Lengend Snippet: DSP-0509 combined anti-PD-1 increased the level of M1-like macrophages in the tumor microenvironment. (A) UMAP representation of monocyte, macrophage and cDC clusters in a population of CD45 + cells derived from CT26 tumors. Data in UMAP includes of all treatment group. (B) Expression profiles of marker genes for myeloid subtypes in macrophage clusters. Data in UMAP includes all treatment group. (C) Frequency of macrophages identified by cluster gating in each treatment group. (D) Comparison of gene expression in cluster 2 cells with that in the cells of other clusters shown by a volcano plot.

Article Snippet: In brief, Tregs (CD4 + CD25 + CD45RB low ) and CD4 + T cells (CD4 + CD25 - CD45RB high ) from the spleens of CT26 tumor-bearing mice were separated by a MoFlo Astrios cell sorter (Beckman Coulter, Inc.) after pre-sorting with mouse CD90.2 MACS beads (Miltenyi Biotec B.V. & Co. KG).

Techniques: Derivative Assay, Expressing, Marker, Comparison

Journal: Cancers

Article Title: WEE1 Inhibitor Adavosertib Exerts Antitumor Effects on Colorectal Cancer, Especially in Cases with p53 Mutations

doi: 10.3390/cancers16183136

Figure Lengend Snippet: CRC cell lines and gene mutation status.

Article Snippet: The BALB/c mouse CRC cell line CT26 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Mutagenesis

Journal: Cancers

Article Title: WEE1 Inhibitor Adavosertib Exerts Antitumor Effects on Colorectal Cancer, Especially in Cases with p53 Mutations

doi: 10.3390/cancers16183136

Figure Lengend Snippet: Effects of the WEE1 inhibitor, adavosertib, on tumor volume in an orthotopic transplanted mouse model of CRC. ( a ) Orthotopic transplanted mouse model of CRC using MC38: transplant design and treatment schedule. n = 5, control group; n = 4, treatment group. ( b ) Macroscopic images of mouse tumors obtained following euthanasia on day 35 post-transplantation. ( c ) Comparison of tumor volume between control and treatment groups. ( d ) Orthotopic transplanted mouse model of CRC using CT26: transplant design and treatment schedule. n = 6, control group; n = 6, treatment group. ( e ) Macroscopic images of mouse tumors obtained following euthanasia on day 24 post-transplantation. ( f ) Comparison of tumor volume between control and treatment groups. Welch’s t -test, **: p < 0.01, n.s.: not significant.

Article Snippet: The BALB/c mouse CRC cell line CT26 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control, Transplantation Assay, Comparison

Journal: Cancers

Article Title: Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route

doi: 10.3390/cancers16172980

Figure Lengend Snippet: Cytotoxic effect of lipophilic cations and 5FU on the cell viability of the SW620 and CT26 cell lines. Changes in cell viability induced by compounds under study in 24, 48, and 72 h of treatment. ( A ) Sigmoidal dose–response curves for TPP + C 1 0 in SW620 and ( B ) CT26 cells. ( C ) Sigmoidal dose–response curves for GA-TPP + C 10 in SW620 and ( D ) CT26 cells. ( E ) Sigmoidal dose–response curves for 5FU in SW620 and ( F ) CT26 cells. Results are expressed as mean ± SD of at least three independent experiments.

Article Snippet: COLO205 (peritoneum metastasis) and SW620 (lymph nodes metastasis) Human metastatic cell lines and CT26 mouse colorectal cancer cells were purchased from ATCC.

Techniques:

Journal: Cancers

Article Title: Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route

doi: 10.3390/cancers16172980

Figure Lengend Snippet: IC 50 of compounds TPP + C 10 and GA-TPP + C 10 in SW620 and CT26 cell lines. Cells were exposed to increasing concentrations and analyzed at 24, 48, and 72 h. Results were estimated from respective sigmoidal dose–response curves. Results are expressed as mean ± SD of at least three independent experiments.

Article Snippet: COLO205 (peritoneum metastasis) and SW620 (lymph nodes metastasis) Human metastatic cell lines and CT26 mouse colorectal cancer cells were purchased from ATCC.

Techniques:

Journal: Cancers

Article Title: Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route

doi: 10.3390/cancers16172980

Figure Lengend Snippet: TPP + C 10 and GA-TPP + C 10 reduce migration in metastatic cell lines. Cells were exposed to the compounds for 24 h at 1 µM and 5 µM. ( A ) Changes in protein levels of MMP-9 and MMP-2 in COLO205 cells. ( B , C ) Quantification of protein levels. ( D , E ) mRNA of MMP-9 and MMP-2, respectively, in COLO205 cells. ( F ) Protein levels of MMP-9 and MMP-2 in SW620 cells. ( G , H ) Quantification of protein levels of MMP-9 and MMP-2, respectively. ( I , J ) mRNA expression of MMP-9 and MMP-2 levels in SW620 cells, respectively. ( K ) Protein levels of MMP-9 and MMP-2 in CT26 cells. ( L , M ) Quantification of protein levels of MMP-9 and MMP-2, respectively. ( N ) Representative picture of transwell migration in CT26 cells with the compounds and respective quantification. Results are expressed as mean ± SD of at least three independent experiments. Statistical differences with control: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: COLO205 (peritoneum metastasis) and SW620 (lymph nodes metastasis) Human metastatic cell lines and CT26 mouse colorectal cancer cells were purchased from ATCC.

Techniques: Migration, Expressing, Control