mouse cd146 gene  (Sino Biological)


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    Name:
    CD146 MCAM cDNA ORF Clone in Cloning Vector Mouse
    Description:
    Full length Clone DNA of Mouse melanoma cell adhesion molecule
    Catalog Number:
    MG50794-G
    Price:
    75.0
    Category:
    cDNA Clone
    Size:
    1Unit
    Product Aliases:
    1-gicerin cDNA ORF Clone Mouse, AV025631 cDNA ORF Clone Mouse, CD146 cDNA ORF Clone Mouse, CD149 cDNA ORF Clone Mouse, Muc18 cDNA ORF Clone Mouse, s-endo cDNA ORF Clone Mouse, s-gicerin cDNA ORF Clone Mouse
    Molecule Name:
    MCAM,s-gicerin,CD146
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    Structured Review

    Sino Biological mouse cd146 gene
    Full length Clone DNA of Mouse melanoma cell adhesion molecule
    https://www.bioz.com/result/mouse cd146 gene/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse cd146 gene - by Bioz Stars, 2021-07
    91/100 stars

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    Article Title: CD146 mediates an E-cadherin-to-N-cadherin switch during TGF-β signaling-induced epithelial-mesenchymal transition.
    Article Snippet: Cadherin switch is an initiating factor of epithelial-mesenchymal transition (EMT) and is intimately correlated with cancer metastatic potential; however, its underlying mechanisms remain unclear.

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    sino biological rna interference cd146
    <t>CD146</t> mediates netrin-1-induced endothelial cell activation. (A – D) HUVECs transfected with control, CD146, UNC5B or VEGFR2 specific siRNA were subjected to proliferation assay (A) , transwell migration assay (B) , tube formation assay (C) and signaling activation assay (D) . Netrin-1 was applied at the indicated concentrations. Note that the specific siRNAs efficiently downregulated the expression of corresponding molecules. For A – C , n = 3 in each group. Data represent 3 independent experiments (means ± SEM). * P
    Rna Interference Cd146, supplied by sino biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna interference cd146/product/sino biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna interference cd146 - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    91
    Sino Biological mouse cd146 gene
    <t>CD146</t> mediates netrin-1-induced endothelial cell activation. (A – D) HUVECs transfected with control, CD146, UNC5B or VEGFR2 specific siRNA were subjected to proliferation assay (A) , transwell migration assay (B) , tube formation assay (C) and signaling activation assay (D) . Netrin-1 was applied at the indicated concentrations. Note that the specific siRNAs efficiently downregulated the expression of corresponding molecules. For A – C , n = 3 in each group. Data represent 3 independent experiments (means ± SEM). * P
    Mouse Cd146 Gene, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse cd146 gene/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse cd146 gene - by Bioz Stars, 2021-07
    91/100 stars
      Buy from Supplier

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    CD146 mediates netrin-1-induced endothelial cell activation. (A – D) HUVECs transfected with control, CD146, UNC5B or VEGFR2 specific siRNA were subjected to proliferation assay (A) , transwell migration assay (B) , tube formation assay (C) and signaling activation assay (D) . Netrin-1 was applied at the indicated concentrations. Note that the specific siRNAs efficiently downregulated the expression of corresponding molecules. For A – C , n = 3 in each group. Data represent 3 independent experiments (means ± SEM). * P

    Journal: Cell Research

    Article Title: CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development

    doi: 10.1038/cr.2015.15

    Figure Lengend Snippet: CD146 mediates netrin-1-induced endothelial cell activation. (A – D) HUVECs transfected with control, CD146, UNC5B or VEGFR2 specific siRNA were subjected to proliferation assay (A) , transwell migration assay (B) , tube formation assay (C) and signaling activation assay (D) . Netrin-1 was applied at the indicated concentrations. Note that the specific siRNAs efficiently downregulated the expression of corresponding molecules. For A – C , n = 3 in each group. Data represent 3 independent experiments (means ± SEM). * P

    Article Snippet: RNA interference CD146- and UNC5B-specific siRNAs were synthesized by Invitrogen using sequences as previously described , .

    Techniques: Activation Assay, Transfection, Proliferation Assay, Transwell Migration Assay, Tube Formation Assay, Expressing

    CD146 is required for netrin-1-induced angiogenesis in mouse models. (A) Aortic rings were prepared from WT or CD146 EC-KO mice. Control or netrin-1 (50 ng/ml) was directly added to the culture medium. (B) The effect of anti-CD146 antibody AA98 was tested in the aortic-ring assay. Control mIgG or AA98 (100 μg/ml) was added to the culture medium in the presence of control or netrin-1 (50 ng/ml). After culturing for 5-6 days, the number of sprouts from each ring was quantified. n = 10 in each group and results are presented as average number of sprouts per ring (means ± SEM). (C) The Matrigel-plug assay for angiogenesis was carried out using WT or CD146 EC-KO mice. The plugs were mixed with control or netrin-1 (200 ng/ml) and then injected subcutaneously into mice in the corresponding groups. (D) The effect of anti-CD146 antibody AA98 on netrin-1-induced angiogenesis was tested in the Matrigel-plug assay. The plugs were pre-mixed with AA98 or control mIgG (100 μg/ml) and injected into the WT mice. 10 days post injection, the Matrigel plugs were sectioned and immunostained with anti-CD31 antibody. The number of blood vessels in each section was scored. n = 5 in each group and results are presented as average number of blood vessels/mm 2 (means ± SEM). Scale bar, 200 μm. * P

    Journal: Cell Research

    Article Title: CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development

    doi: 10.1038/cr.2015.15

    Figure Lengend Snippet: CD146 is required for netrin-1-induced angiogenesis in mouse models. (A) Aortic rings were prepared from WT or CD146 EC-KO mice. Control or netrin-1 (50 ng/ml) was directly added to the culture medium. (B) The effect of anti-CD146 antibody AA98 was tested in the aortic-ring assay. Control mIgG or AA98 (100 μg/ml) was added to the culture medium in the presence of control or netrin-1 (50 ng/ml). After culturing for 5-6 days, the number of sprouts from each ring was quantified. n = 10 in each group and results are presented as average number of sprouts per ring (means ± SEM). (C) The Matrigel-plug assay for angiogenesis was carried out using WT or CD146 EC-KO mice. The plugs were mixed with control or netrin-1 (200 ng/ml) and then injected subcutaneously into mice in the corresponding groups. (D) The effect of anti-CD146 antibody AA98 on netrin-1-induced angiogenesis was tested in the Matrigel-plug assay. The plugs were pre-mixed with AA98 or control mIgG (100 μg/ml) and injected into the WT mice. 10 days post injection, the Matrigel plugs were sectioned and immunostained with anti-CD31 antibody. The number of blood vessels in each section was scored. n = 5 in each group and results are presented as average number of blood vessels/mm 2 (means ± SEM). Scale bar, 200 μm. * P

    Article Snippet: RNA interference CD146- and UNC5B-specific siRNAs were synthesized by Invitrogen using sequences as previously described , .

    Techniques: Mouse Assay, Aortic Ring Assay, Matrigel Assay, Injection

    Identification of interacting domains of netrin-1 and CD146. (A) A diagrammatic illustration of the full-length (FL) and truncation mutants of netrin-1 expressed as His-tagged proteins. (B) HEK293 cells were co-transfected with plasmid expressing CD146 and the FL or truncation mutant of netrin-1. The cell lysates were prepared for immunoprecipitation with anti-CD146 mAb AA1. (C , D) Defining the domain of CD146 required for interaction with netrin-1. CD146 and its truncation mutants were expressed as Flag-tagged proteins. Immunoprecipitation was performed using anti-netrin-1 mAb. Data represent 3 independent experiments.

    Journal: Cell Research

    Article Title: CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development

    doi: 10.1038/cr.2015.15

    Figure Lengend Snippet: Identification of interacting domains of netrin-1 and CD146. (A) A diagrammatic illustration of the full-length (FL) and truncation mutants of netrin-1 expressed as His-tagged proteins. (B) HEK293 cells were co-transfected with plasmid expressing CD146 and the FL or truncation mutant of netrin-1. The cell lysates were prepared for immunoprecipitation with anti-CD146 mAb AA1. (C , D) Defining the domain of CD146 required for interaction with netrin-1. CD146 and its truncation mutants were expressed as Flag-tagged proteins. Immunoprecipitation was performed using anti-netrin-1 mAb. Data represent 3 independent experiments.

    Article Snippet: RNA interference CD146- and UNC5B-specific siRNAs were synthesized by Invitrogen using sequences as previously described , .

    Techniques: Transfection, Plasmid Preparation, Expressing, Mutagenesis, Immunoprecipitation

    Netrin-1 exhibits dual activities in endothelial cell activation. (A-C) HUVECs were used for proliferation assay (A) , transwell migration assay (B) and tube formation assay (C) . Netrin-1 was applied at concentrations as indicated. (D) serum-starved HUVECs were treated with netrin-1 at different concentrations for 10 min. Dimerization of CD146 and phosphorylation of VEGFR2, ERK1/2 and p38 were analyzed by western blotting. The black arrow marks the CD146 dimer. (E) Representative images of netrin-1-induced HUVEC migration (left) and tube formation (right). For A-C , n = 3 in each group. Data represent 3 independent experiments (means ± SEM). * P

    Journal: Cell Research

    Article Title: CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development

    doi: 10.1038/cr.2015.15

    Figure Lengend Snippet: Netrin-1 exhibits dual activities in endothelial cell activation. (A-C) HUVECs were used for proliferation assay (A) , transwell migration assay (B) and tube formation assay (C) . Netrin-1 was applied at concentrations as indicated. (D) serum-starved HUVECs were treated with netrin-1 at different concentrations for 10 min. Dimerization of CD146 and phosphorylation of VEGFR2, ERK1/2 and p38 were analyzed by western blotting. The black arrow marks the CD146 dimer. (E) Representative images of netrin-1-induced HUVEC migration (left) and tube formation (right). For A-C , n = 3 in each group. Data represent 3 independent experiments (means ± SEM). * P

    Article Snippet: RNA interference CD146- and UNC5B-specific siRNAs were synthesized by Invitrogen using sequences as previously described , .

    Techniques: Activation Assay, Proliferation Assay, Transwell Migration Assay, Tube Formation Assay, Western Blot, Migration

    Netrin-1 binds to CD146. (A) Co-immunoprecipitation assays. Netrin-1- and CD146-expressing plasmids were co-transfected into HEK293 cells prior to preparation of cell lysates. Lanes 1 and 4: precipitated by control mIgG. Lanes 2 and 3: anti-CD146 mAb AA1. Lanes 5 and 6: anti-netrin-1 mAb. (B) HEK293 cells were transfected with plasmids encoding DCC, CD146 or Robo-1 and incubated with netrin-1-GFP conditional medium. Binding of netrin-1-GFP to the cell was detected by its GFP fluorescence. Scale bar, 50 μm. (C) Direct interaction between purified netrin-1 and CD146 proteins in vitro . Fc, Fc-CD146 or Fc-UNC5B (200 ng/ml) bound protein G beads was incubated with netrin-1 protein (200 ng/ml). The bound proteins were analyzed by western blotting. (D , E) Determination of netrin-CD146 binding affinity by SPR. Purified netrin-1 protein was applied at different concentrations to the CM5 chips containing Fc-CD146 (D) or Fc-UNC5B (E) . Data represent 3 independent experiments.

    Journal: Cell Research

    Article Title: CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development

    doi: 10.1038/cr.2015.15

    Figure Lengend Snippet: Netrin-1 binds to CD146. (A) Co-immunoprecipitation assays. Netrin-1- and CD146-expressing plasmids were co-transfected into HEK293 cells prior to preparation of cell lysates. Lanes 1 and 4: precipitated by control mIgG. Lanes 2 and 3: anti-CD146 mAb AA1. Lanes 5 and 6: anti-netrin-1 mAb. (B) HEK293 cells were transfected with plasmids encoding DCC, CD146 or Robo-1 and incubated with netrin-1-GFP conditional medium. Binding of netrin-1-GFP to the cell was detected by its GFP fluorescence. Scale bar, 50 μm. (C) Direct interaction between purified netrin-1 and CD146 proteins in vitro . Fc, Fc-CD146 or Fc-UNC5B (200 ng/ml) bound protein G beads was incubated with netrin-1 protein (200 ng/ml). The bound proteins were analyzed by western blotting. (D , E) Determination of netrin-CD146 binding affinity by SPR. Purified netrin-1 protein was applied at different concentrations to the CM5 chips containing Fc-CD146 (D) or Fc-UNC5B (E) . Data represent 3 independent experiments.

    Article Snippet: RNA interference CD146- and UNC5B-specific siRNAs were synthesized by Invitrogen using sequences as previously described , .

    Techniques: Immunoprecipitation, Expressing, Transfection, Droplet Countercurrent Chromatography, Incubation, Binding Assay, Fluorescence, Purification, In Vitro, Western Blot, SPR Assay

    CD146 mediates netrin-1-induced angiogenesis in zebrafish. (A) CD146 expression in the zebrafish embryos at different stages was detected by whole mount in situ hybridization. (B) The control, CD146 or netrin-1a specific MO was injected with control or human netrin-1 mRNA into the transgenic zebrafish Tg(kdrl:GFP) embryos that expressed GFP to enable visualization of vasculature. The vascular patterns of the fish embryos at 36, 48 and 60 hpf were analyzed. Yellow arrowheads indicate PAV. Red arrowheads mark ectopic sprouts in netrin-1 mRNA-injected embryos. (C) Tg(Fli1:nGFP) × Tg(kdrl:mcherry) embryos were injected with the indicated MO and mRNA. The number of endothelial cells in the ISVs of the embryos at 36 hpf, 48 hpf and 60 hpf stages was quantified and analyzed. Data are presented as average number of endothelial cells per ISV (means ± SEM). * P

    Journal: Cell Research

    Article Title: CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development

    doi: 10.1038/cr.2015.15

    Figure Lengend Snippet: CD146 mediates netrin-1-induced angiogenesis in zebrafish. (A) CD146 expression in the zebrafish embryos at different stages was detected by whole mount in situ hybridization. (B) The control, CD146 or netrin-1a specific MO was injected with control or human netrin-1 mRNA into the transgenic zebrafish Tg(kdrl:GFP) embryos that expressed GFP to enable visualization of vasculature. The vascular patterns of the fish embryos at 36, 48 and 60 hpf were analyzed. Yellow arrowheads indicate PAV. Red arrowheads mark ectopic sprouts in netrin-1 mRNA-injected embryos. (C) Tg(Fli1:nGFP) × Tg(kdrl:mcherry) embryos were injected with the indicated MO and mRNA. The number of endothelial cells in the ISVs of the embryos at 36 hpf, 48 hpf and 60 hpf stages was quantified and analyzed. Data are presented as average number of endothelial cells per ISV (means ± SEM). * P

    Article Snippet: RNA interference CD146- and UNC5B-specific siRNAs were synthesized by Invitrogen using sequences as previously described , .

    Techniques: Expressing, In Situ Hybridization, Injection, Transgenic Assay, Fluorescence In Situ Hybridization