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mouse c2c12 undifferentiated myoblast cells  (ATCC)


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    ATCC mouse c2c12 undifferentiated myoblast cells
    Mouse C2c12 Undifferentiated Myoblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse c2c12 undifferentiated myoblast cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    mouse c2c12 undifferentiated myoblast cells - by Bioz Stars, 2025-07
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    undifferentiated mouse myoblast c2c12 cells  (ATCC)
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    ATCC undifferentiated mouse myoblast c2c12 cells
    ( A ) Fluorescence images of MNIM-EOH cells at 1 day after cocultured with <t>C2C12</t> myotubes. α-BTX staining revealed AChR clustering on C2C12 muscle fibers. Confocal Z-stack imaging, in a line through the region of apparent colocalization, confirmed EGFP+ axons in close proximity to AChRs (arrows). Scale bars: 10 µm. ( B ) End-plate currents (EPCs) were recorded from myotubes located in close proximity to MNIM-EOH cells. EPCs were blocked from the same cell after application of 15 µM pancuronium. However, after washing out of pancuronium, EPCs could be recorded again. The bars represent mean±SEM. * P <0.05. The significance was determined by Student’s t test.
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    ( A ) Fluorescence images of MNIM-EOH cells at 1 day after cocultured with C2C12 myotubes. α-BTX staining revealed AChR clustering on C2C12 muscle fibers. Confocal Z-stack imaging, in a line through the region of apparent colocalization, confirmed EGFP+ axons in close proximity to AChRs (arrows). Scale bars: 10 µm. ( B ) End-plate currents (EPCs) were recorded from myotubes located in close proximity to MNIM-EOH cells. EPCs were blocked from the same cell after application of 15 µM pancuronium. However, after washing out of pancuronium, EPCs could be recorded again. The bars represent mean±SEM. * P <0.05. The significance was determined by Student’s t test.

    Journal: PLoS ONE

    Article Title: Directed Induction of Functional Motor Neuron-Like Cells from Genetically Engineered Human Mesenchymal Stem Cells

    doi: 10.1371/journal.pone.0035244

    Figure Lengend Snippet: ( A ) Fluorescence images of MNIM-EOH cells at 1 day after cocultured with C2C12 myotubes. α-BTX staining revealed AChR clustering on C2C12 muscle fibers. Confocal Z-stack imaging, in a line through the region of apparent colocalization, confirmed EGFP+ axons in close proximity to AChRs (arrows). Scale bars: 10 µm. ( B ) End-plate currents (EPCs) were recorded from myotubes located in close proximity to MNIM-EOH cells. EPCs were blocked from the same cell after application of 15 µM pancuronium. However, after washing out of pancuronium, EPCs could be recorded again. The bars represent mean±SEM. * P <0.05. The significance was determined by Student’s t test.

    Article Snippet: Undifferentiated mouse myoblast C2C12 cells (American Type Culture Collection, Manassas, VA) were maintained in DMEM with 10% FBS, penicillin and streptomycin.

    Techniques: Fluorescence, Staining, Imaging