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mouse c2c12 myoblast cell line rcb0987  (BioResource International Inc)

 
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    BioResource International Inc mouse c2c12 myoblast cell line rcb0987
    Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) <t>C2C12</t> myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.
    Mouse C2c12 Myoblast Cell Line Rcb0987, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse c2c12 myoblast cell line rcb0987/product/BioResource International Inc
    Average 86 stars, based on 1 article reviews
    mouse c2c12 myoblast cell line rcb0987 - by Bioz Stars, 2025-07
    86/100 stars

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    1) Product Images from "Glucose, glutamine, lactic acid and α-ketoglutarate restore metabolic disturbances and atrophic changes in energy-deprived muscle cells"

    Article Title: Glucose, glutamine, lactic acid and α-ketoglutarate restore metabolic disturbances and atrophic changes in energy-deprived muscle cells

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2025.13562

    Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) C2C12 myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.
    Figure Legend Snippet: Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) C2C12 myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.

    Techniques Used: Activity Assay, Incubation, Cell Culture, MTT Assay, Control

    Effect of single nutrient supplementation on ATP production. C2C12 myotubes were incubated in starvation medium with or without the indicated nutrients for 24 h. The ATP production rates from glycolysis (Glyco-ATP) and OxPhos (OxPhos-ATP) were determined. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as mean ± SEM (n=3-4). **P<0.01, ***P<0.001 vs. control cells for total ATP production; §§§ P<0.001 vs. starved cells for Glyco-ATP production. Con, control; Glyco-ATP, glycolytic ATP; OxPhos, oxidative phosphorylation; Stv, starvation.
    Figure Legend Snippet: Effect of single nutrient supplementation on ATP production. C2C12 myotubes were incubated in starvation medium with or without the indicated nutrients for 24 h. The ATP production rates from glycolysis (Glyco-ATP) and OxPhos (OxPhos-ATP) were determined. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as mean ± SEM (n=3-4). **P<0.01, ***P<0.001 vs. control cells for total ATP production; §§§ P<0.001 vs. starved cells for Glyco-ATP production. Con, control; Glyco-ATP, glycolytic ATP; OxPhos, oxidative phosphorylation; Stv, starvation.

    Techniques Used: Incubation, Control, Phospho-proteomics



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    mouse c2c12 myoblast cell line rcb0987  (BioResource International Inc)
    86
    BioResource International Inc mouse c2c12 myoblast cell line rcb0987
    Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) <t>C2C12</t> myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.
    Mouse C2c12 Myoblast Cell Line Rcb0987, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse c2c12 myoblast cell line rcb0987/product/BioResource International Inc
    Average 86 stars, based on 1 article reviews
    mouse c2c12 myoblast cell line rcb0987 - by Bioz Stars, 2025-07
    86/100 stars
    		  Buy from Supplier
    mouse myoblast cell line c2c12 rcb0987  (BioResource International Inc)
    86
    BioResource International Inc mouse myoblast cell line c2c12 rcb0987
    Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) <t>C2C12</t> myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.
    Mouse Myoblast Cell Line C2c12 Rcb0987, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse myoblast cell line c2c12 rcb0987/product/BioResource International Inc
    Average 86 stars, based on 1 article reviews
    mouse myoblast cell line c2c12 rcb0987 - by Bioz Stars, 2025-07
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) C2C12 myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.

    Journal: Molecular Medicine Reports

    Article Title: Glucose, glutamine, lactic acid and α-ketoglutarate restore metabolic disturbances and atrophic changes in energy-deprived muscle cells

    doi: 10.3892/mmr.2025.13562

    Figure Lengend Snippet: Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) C2C12 myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.

    Article Snippet: Mouse C2C12 myoblast cell line (RCB0987) was purchased from the RIKEN BioResource Research Center (Tsukuba, Japan).

    Techniques: Activity Assay, Incubation, Cell Culture, MTT Assay, Control

    Effect of single nutrient supplementation on ATP production. C2C12 myotubes were incubated in starvation medium with or without the indicated nutrients for 24 h. The ATP production rates from glycolysis (Glyco-ATP) and OxPhos (OxPhos-ATP) were determined. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as mean ± SEM (n=3-4). **P<0.01, ***P<0.001 vs. control cells for total ATP production; §§§ P<0.001 vs. starved cells for Glyco-ATP production. Con, control; Glyco-ATP, glycolytic ATP; OxPhos, oxidative phosphorylation; Stv, starvation.

    Journal: Molecular Medicine Reports

    Article Title: Glucose, glutamine, lactic acid and α-ketoglutarate restore metabolic disturbances and atrophic changes in energy-deprived muscle cells

    doi: 10.3892/mmr.2025.13562

    Figure Lengend Snippet: Effect of single nutrient supplementation on ATP production. C2C12 myotubes were incubated in starvation medium with or without the indicated nutrients for 24 h. The ATP production rates from glycolysis (Glyco-ATP) and OxPhos (OxPhos-ATP) were determined. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as mean ± SEM (n=3-4). **P<0.01, ***P<0.001 vs. control cells for total ATP production; §§§ P<0.001 vs. starved cells for Glyco-ATP production. Con, control; Glyco-ATP, glycolytic ATP; OxPhos, oxidative phosphorylation; Stv, starvation.

    Article Snippet: Mouse C2C12 myoblast cell line (RCB0987) was purchased from the RIKEN BioResource Research Center (Tsukuba, Japan).

    Techniques: Incubation, Control, Phospho-proteomics