inducible nitric oxide synthase  (Boster Bio)

 
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  • 93
    Name:
    Anti Nitric oxide synthase inducible Antibody
    Description:
    Rabbit Polyclonal antibody for Nos2 detection Tested positive for IF IHC WB in Human Mouse Rat
    Catalog Number:
    A00368-1
    Price:
    462.0
    Applications:
    IF, IHC, WB
    Conjugate:
    No
    Immunogen:
    Synthetic peptide corresponding to aa 1131-1141 of mouse macrophage NOS.
    Size:
    100µl
    Category:
    Primary Antibodies
    Reactivity:
    Human Mouse Rat
    Buy from Supplier


    Structured Review

    Boster Bio inducible nitric oxide synthase
    Effects of celastrol (2 mg/kg) on the levels of inflammation cytokines and proteins in AOM/DSS-treated mice. (A) The levels of tumor necrosis factor-α (TNF-α) (a), interleukin (IL)-1β (b), and IL-6 (c) in serum from the control mice, AOM/DSS model mice and AOM/DSS in combination with celastrol treated mice were determined using ELISA assay. (B) The expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide <t>synthase</t> (iNOS) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Representative stained colonic sections from each group are shown. Original magnification was 400×. (C) The expression levels of COX-2 and iNOS as well as cytoplasmic and nuclear NF-κB p65 in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). C , cytoplasm; N , nuclear. Data are presented as mean ± SD ( n = 6). ∗∗ p
    Rabbit Polyclonal antibody for Nos2 detection Tested positive for IF IHC WB in Human Mouse Rat
    https://www.bioz.com/result/inducible nitric oxide synthase/product/Boster Bio
    Average 93 stars, based on 548 article reviews
    Price from $9.99 to $1999.99
    inducible nitric oxide synthase - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition"

    Article Title: Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2015.00320

    Effects of celastrol (2 mg/kg) on the levels of inflammation cytokines and proteins in AOM/DSS-treated mice. (A) The levels of tumor necrosis factor-α (TNF-α) (a), interleukin (IL)-1β (b), and IL-6 (c) in serum from the control mice, AOM/DSS model mice and AOM/DSS in combination with celastrol treated mice were determined using ELISA assay. (B) The expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Representative stained colonic sections from each group are shown. Original magnification was 400×. (C) The expression levels of COX-2 and iNOS as well as cytoplasmic and nuclear NF-κB p65 in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). C , cytoplasm; N , nuclear. Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol (2 mg/kg) on the levels of inflammation cytokines and proteins in AOM/DSS-treated mice. (A) The levels of tumor necrosis factor-α (TNF-α) (a), interleukin (IL)-1β (b), and IL-6 (c) in serum from the control mice, AOM/DSS model mice and AOM/DSS in combination with celastrol treated mice were determined using ELISA assay. (B) The expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Representative stained colonic sections from each group are shown. Original magnification was 400×. (C) The expression levels of COX-2 and iNOS as well as cytoplasmic and nuclear NF-κB p65 in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). C , cytoplasm; N , nuclear. Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Staining, Western Blot

    2) Product Images from "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition"

    Article Title: Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2015.00320

    Effects of celastrol (2 mg/kg) on the levels of inflammation cytokines and proteins in AOM/DSS-treated mice. (A) The levels of tumor necrosis factor-α (TNF-α) (a), interleukin (IL)-1β (b), and IL-6 (c) in serum from the control mice, AOM/DSS model mice and AOM/DSS in combination with celastrol treated mice were determined using ELISA assay. (B) The expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Representative stained colonic sections from each group are shown. Original magnification was 400×. (C) The expression levels of COX-2 and iNOS as well as cytoplasmic and nuclear NF-κB p65 in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). C , cytoplasm; N , nuclear. Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol (2 mg/kg) on the levels of inflammation cytokines and proteins in AOM/DSS-treated mice. (A) The levels of tumor necrosis factor-α (TNF-α) (a), interleukin (IL)-1β (b), and IL-6 (c) in serum from the control mice, AOM/DSS model mice and AOM/DSS in combination with celastrol treated mice were determined using ELISA assay. (B) The expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Representative stained colonic sections from each group are shown. Original magnification was 400×. (C) The expression levels of COX-2 and iNOS as well as cytoplasmic and nuclear NF-κB p65 in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). C , cytoplasm; N , nuclear. Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Staining, Western Blot

    3) Product Images from "Identification and differentiation therapy strategy of pterygium in vitro"

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    Journal: American Journal of Translational Research

    doi:

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.
    Figure Legend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Techniques Used:

    4) Product Images from "LncRNA TUG1 regulates autophagy-mediated endothelial-mesenchymal transition of liver sinusoidal endothelial cells by sponging miR-142-3p"

    Article Title: LncRNA TUG1 regulates autophagy-mediated endothelial-mesenchymal transition of liver sinusoidal endothelial cells by sponging miR-142-3p

    Journal: American Journal of Translational Research

    doi:

    Increased autophagy and EndMT of LSECs in a rat model of cirrhosis. A. Elevated serum LPS levels were detected in cirrhotic rats. B. Pathological changes in CCl4-induced liver cirrhosis were examined by H E and Masson’s trichrome. The expression of autophagy and EndMT proteins (LC3B, CD31, α-SMA, and vimentin) in normal and cirrhotic rats was examined by immunohistochemical staining. **, P
    Figure Legend Snippet: Increased autophagy and EndMT of LSECs in a rat model of cirrhosis. A. Elevated serum LPS levels were detected in cirrhotic rats. B. Pathological changes in CCl4-induced liver cirrhosis were examined by H E and Masson’s trichrome. The expression of autophagy and EndMT proteins (LC3B, CD31, α-SMA, and vimentin) in normal and cirrhotic rats was examined by immunohistochemical staining. **, P

    Techniques Used: Expressing, Immunohistochemistry, Staining

    5) Product Images from "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition"

    Article Title: Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2015.00320

    Effects of celastrol (2 mg/kg) on the oncogenic protein expression in AOM/DSS-treated mice. (A) The expression of p53, p-p53, β-catenin, and proliferating cell nuclear antigen (PCNA) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Shown are representative section of colon tissues from the control group, UC-CRC model group and celastrol-treated group. Original magnification was 400×. (B) The expression levels of p53, p-p53, β-catenin, and PCNA in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol (2 mg/kg) on the oncogenic protein expression in AOM/DSS-treated mice. (A) The expression of p53, p-p53, β-catenin, and proliferating cell nuclear antigen (PCNA) in normal colonic tissues or colonic tumor tissues was evaluated using immunohistochemical staining. Shown are representative section of colon tissues from the control group, UC-CRC model group and celastrol-treated group. Original magnification was 400×. (B) The expression levels of p53, p-p53, β-catenin, and PCNA in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Western Blot

    6) Product Images from "Identification and differentiation therapy strategy of pterygium in vitro"

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    Journal: American Journal of Translational Research

    doi:

    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.
    Figure Legend Snippet: Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.

    Techniques Used: Staining, Immunohistochemistry

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.
    Figure Legend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Techniques Used:

    7) Product Images from "A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos"

    Article Title: A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos

    Journal: Cytotechnology

    doi: 10.1007/s10616-014-9805-1

    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)
    Figure Legend Snippet: Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Techniques Used: Expressing, Marker, Derivative Assay, Staining, Negative Control

    8) Product Images from "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition"

    Article Title: Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2015.00320

    Effects of celastrol on the expression levels of EMT-related proteins in xenograft CRC mice. The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 tumors and HT-29 tumors from each group were determined by western blot analysis. Representative bands are shown (Left) , and the relative band intensity ratio was analyzed (Right) . Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol on the expression levels of EMT-related proteins in xenograft CRC mice. The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 tumors and HT-29 tumors from each group were determined by western blot analysis. Representative bands are shown (Left) , and the relative band intensity ratio was analyzed (Right) . Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Expressing, Mouse Assay, Western Blot

    Effects of celastrol (2 mg/kg) on the expression levels of EMT-related proteins in AOM/DSS-treated mice. (A) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in normal colonic tissues or colonic tumor tissues was assessed using immunohistochemical staining. Representative colonic sections from the control group, UC-CRC model group and celastrol-treated group are shown. Original magnification was 400×. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol (2 mg/kg) on the expression levels of EMT-related proteins in AOM/DSS-treated mice. (A) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in normal colonic tissues or colonic tumor tissues was assessed using immunohistochemical staining. Representative colonic sections from the control group, UC-CRC model group and celastrol-treated group are shown. Original magnification was 400×. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Western Blot

    Effects of celastrol on cell proliferation and EMT-related protein expression in CRC cells in vitro . (A) HCT116 (a) and HT-29 (b) cells were exposed to celastrol (0, 20, and 40 μM) for 24 and 48 h, and cell viability was determined by MTT assay. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 and HT-29 cells treated with celastrol (0–40 μM) for 48 h were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD from three independent experiments. ∗ p
    Figure Legend Snippet: Effects of celastrol on cell proliferation and EMT-related protein expression in CRC cells in vitro . (A) HCT116 (a) and HT-29 (b) cells were exposed to celastrol (0, 20, and 40 μM) for 24 and 48 h, and cell viability was determined by MTT assay. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 and HT-29 cells treated with celastrol (0–40 μM) for 48 h were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD from three independent experiments. ∗ p

    Techniques Used: Expressing, In Vitro, MTT Assay, Western Blot

    9) Product Images from "Identification and differentiation therapy strategy of pterygium in vitro"

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    Journal: American Journal of Translational Research

    doi:

    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.
    Figure Legend Snippet: Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.

    Techniques Used: Staining, Immunohistochemistry

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.
    Figure Legend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Techniques Used:

    10) Product Images from "Identification and differentiation therapy strategy of pterygium in vitro"

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    Journal: American Journal of Translational Research

    doi:

    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.
    Figure Legend Snippet: Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.

    Techniques Used: Staining, Immunohistochemistry

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.
    Figure Legend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Techniques Used:

    11) Product Images from "The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency"

    Article Title: The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency

    Journal: Stem Cells International

    doi: 10.1155/2016/4148923

    Identification and characterization of isolated hAECs. (a) The representative characteristics of hAECs under light microscope. (b) Expression of  Oct-4, Nanog,  and  Sox2  (relative to a beta-actin internal control) in cultured hAECs by real-time PCR analysis. The results presented were the average values from five different donors. (c) The analysis of epithelial and mesenchymal markers expression (relative to a beta-actin internal control) in isolated hAECs by real-time PCR. The results presented were the average values from five different donors. (d) Immunofluorescence detection of Cytokeratin 18, CD34, and Vimentin in cultured hAECs. Scale bar: 200  μ m.
    Figure Legend Snippet: Identification and characterization of isolated hAECs. (a) The representative characteristics of hAECs under light microscope. (b) Expression of Oct-4, Nanog, and Sox2 (relative to a beta-actin internal control) in cultured hAECs by real-time PCR analysis. The results presented were the average values from five different donors. (c) The analysis of epithelial and mesenchymal markers expression (relative to a beta-actin internal control) in isolated hAECs by real-time PCR. The results presented were the average values from five different donors. (d) Immunofluorescence detection of Cytokeratin 18, CD34, and Vimentin in cultured hAECs. Scale bar: 200  μ m.

    Techniques Used: Isolation, Light Microscopy, Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Immunofluorescence

    12) Product Images from "Substance P Inhibits the Collagen Synthesis of Rat Myocardial Fibroblasts Induced by Ang II"

    Article Title: Substance P Inhibits the Collagen Synthesis of Rat Myocardial Fibroblasts Induced by Ang II

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.898454

    Identification of CFBs in vitro . ( A ) Observation of cell state and morphology in inverted microscope. ( B ) Immunofluorescence staining detected vimentin (green) and vascular smooth muscle actin (red) in isolation and culture CFBs. Scale bar=100 μm.
    Figure Legend Snippet: Identification of CFBs in vitro . ( A ) Observation of cell state and morphology in inverted microscope. ( B ) Immunofluorescence staining detected vimentin (green) and vascular smooth muscle actin (red) in isolation and culture CFBs. Scale bar=100 μm.

    Techniques Used: In Vitro, Inverted Microscopy, Immunofluorescence, Staining, Isolation

    13) Product Images from "Identification and differentiation therapy strategy of pterygium in vitro"

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    Journal: American Journal of Translational Research

    doi:

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.
    Figure Legend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Techniques Used:

    14) Product Images from "Corticosterone suppresses IL-1β-induced mPGE2 expression through regulation of the 11β-HSD1 bioactivity of synovial fibroblasts in vitro"

    Article Title: Corticosterone suppresses IL-1β-induced mPGE2 expression through regulation of the 11β-HSD1 bioactivity of synovial fibroblasts in vitro

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2017.4238

    Evaluation of isolated synovial fibroblasts from passage 3 using vimentin staining and CD90 detection by flow cytometry. (A and B) Isolated cells from synovial membranes of Sprague-Dawley rat knees (magnification, ×40). (C) Vimentin in the synovial cells was stained brown (magnification, ×100; immunocytochemical staining with diaminobenzidine chromogen). (D) PBS was used instead of vimentin staining to act as a negative control (magnification, ×100). (E) Cells were marked with homotype-negative phycoerythrin-labeled IgG antibody (negative control). (F) Positive cells marked with CD90 were detected by flow cytometry and indicated that the phenotype conformed to synovial fibroblasts. CD90, cluster of differentiation-90; IgG, immunoglobulin G.
    Figure Legend Snippet: Evaluation of isolated synovial fibroblasts from passage 3 using vimentin staining and CD90 detection by flow cytometry. (A and B) Isolated cells from synovial membranes of Sprague-Dawley rat knees (magnification, ×40). (C) Vimentin in the synovial cells was stained brown (magnification, ×100; immunocytochemical staining with diaminobenzidine chromogen). (D) PBS was used instead of vimentin staining to act as a negative control (magnification, ×100). (E) Cells were marked with homotype-negative phycoerythrin-labeled IgG antibody (negative control). (F) Positive cells marked with CD90 were detected by flow cytometry and indicated that the phenotype conformed to synovial fibroblasts. CD90, cluster of differentiation-90; IgG, immunoglobulin G.

    Techniques Used: Isolation, Staining, Flow Cytometry, Cytometry, Activated Clotting Time Assay, Negative Control, Labeling

    15) Product Images from "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition"

    Article Title: Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2015.00320

    Effects of celastrol on the expression levels of EMT-related proteins in xenograft CRC mice. The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 tumors and HT-29 tumors from each group were determined by western blot analysis. Representative bands are shown (Left) , and the relative band intensity ratio was analyzed (Right) . Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol on the expression levels of EMT-related proteins in xenograft CRC mice. The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 tumors and HT-29 tumors from each group were determined by western blot analysis. Representative bands are shown (Left) , and the relative band intensity ratio was analyzed (Right) . Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Expressing, Mouse Assay, Western Blot

    Effects of celastrol (2 mg/kg) on the expression levels of EMT-related proteins in AOM/DSS-treated mice. (A) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in normal colonic tissues or colonic tumor tissues was assessed using immunohistochemical staining. Representative colonic sections from the control group, UC-CRC model group and celastrol-treated group are shown. Original magnification was 400×. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD ( n = 6). ∗∗ p
    Figure Legend Snippet: Effects of celastrol (2 mg/kg) on the expression levels of EMT-related proteins in AOM/DSS-treated mice. (A) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in normal colonic tissues or colonic tumor tissues was assessed using immunohistochemical staining. Representative colonic sections from the control group, UC-CRC model group and celastrol-treated group are shown. Original magnification was 400×. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in colonic tissues from each group were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD ( n = 6). ∗∗ p

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Western Blot

    Effects of celastrol on cell proliferation and EMT-related protein expression in CRC cells in vitro . (A) HCT116 (a) and HT-29 (b) cells were exposed to celastrol (0, 20, and 40 μM) for 24 and 48 h, and cell viability was determined by MTT assay. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 and HT-29 cells treated with celastrol (0–40 μM) for 48 h were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD from three independent experiments. ∗ p
    Figure Legend Snippet: Effects of celastrol on cell proliferation and EMT-related protein expression in CRC cells in vitro . (A) HCT116 (a) and HT-29 (b) cells were exposed to celastrol (0, 20, and 40 μM) for 24 and 48 h, and cell viability was determined by MTT assay. (B) The expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in HCT116 and HT-29 cells treated with celastrol (0–40 μM) for 48 h were determined by western blot analysis. Representative bands are shown (left), and the relative band intensity ratio was analyzed (right). Data are presented as mean ± SD from three independent experiments. ∗ p

    Techniques Used: Expressing, In Vitro, MTT Assay, Western Blot

    16) Product Images from "Identification and differentiation therapy strategy of pterygium in vitro"

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    Journal: American Journal of Translational Research

    doi:

    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.
    Figure Legend Snippet: Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.

    Techniques Used: Staining, Immunohistochemistry

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.
    Figure Legend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Techniques Used:

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    Incubation:

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    Immunohistochemistry:

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    Boster Bio mouse anti vimentin antibody solution
    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of <t>vimentin</t> for fibroblasts, b negative control for vimentin (50 µm)
    Mouse Anti Vimentin Antibody Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Journal: Cytotechnology

    Article Title: A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos

    doi: 10.1007/s10616-014-9805-1

    Figure Lengend Snippet: Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Article Snippet: Samples were then treated with an acetone–methanol (1:1) solution for 15–20 min at room temperature and incubated in a penetrant solution (PBS with 1 % BSA and 0.1 % Tween-20) for 1 h. Subsequently, these were incubated in mouse anti-vimentin antibody solution (1:200 diluted with PBS; BM0135, BOSTER, Pleasanton, CA, USA) and mouse anti-keratin antibody solution (1:200 diluted with PBS; BM0030, BOSTER) overnight at 4 °C.

    Techniques: Expressing, Marker, Derivative Assay, Staining, Negative Control