apc efluor 780 rat monoclonal anti mouse cd3  (Thermo Fisher)


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    Thermo Fisher apc efluor 780 rat monoclonal anti mouse cd3
    KEY RESOURCES TABLE
    Apc Efluor 780 Rat Monoclonal Anti Mouse Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc efluor 780 rat monoclonal anti mouse cd3/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apc efluor 780 rat monoclonal anti mouse cd3 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Type I interferon decreases macrophage energy metabolism during mycobacterial infection"

    Article Title: Type I interferon decreases macrophage energy metabolism during mycobacterial infection

    Journal: Cell reports

    doi: 10.1016/j.celrep.2021.109195

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Functional Assay, Recombinant, Electron Microscopy, Protease Inhibitor, CyQUANT Assay, LDH Cytotoxicity Assay, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Staining, RNA Sequencing Assay, Software

    mouse anti rat cd3 apc  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
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    Thermo Fisher mouse anti rat cd3 apc
    Activated T follicular helper cells drive GC formation. (A) immunofluorescence staining of a splenic follicle with T cell zone <t>(CD3</t> + , red), GC (Ki67 + , circled green), B cell follicle (CD20 + , yellow) with mantle zone (MZ, circled yellow) and Tfh (white arrows). (B) average number of activated Tfh <t>(CD3</t> + cells in Ki67 + GC area) and resting Tfh (CD3 + cells in CD20 + MZ area) in controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) Correlation of the number of activated Tfh and GC expansion within the loCNI group. (D) plasma cells measured as IgG + cells in spleen sections from controls (n=6), hiCNI (n=6) and loCNI (n=8), and (E) CD45R + CD27 + memory B cells measured by flow cytometry in controls (n=5), hiCNI (n=6) and loCNI (n=4). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05.
    Mouse Anti Rat Cd3 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti rat cd3 apc/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti rat cd3 apc - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose"

    Article Title: Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.657894

    Activated T follicular helper cells drive GC formation. (A) immunofluorescence staining of a splenic follicle with T cell zone (CD3 + , red), GC (Ki67 + , circled green), B cell follicle (CD20 + , yellow) with mantle zone (MZ, circled yellow) and Tfh (white arrows). (B) average number of activated Tfh (CD3 + cells in Ki67 + GC area) and resting Tfh (CD3 + cells in CD20 + MZ area) in controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) Correlation of the number of activated Tfh and GC expansion within the loCNI group. (D) plasma cells measured as IgG + cells in spleen sections from controls (n=6), hiCNI (n=6) and loCNI (n=8), and (E) CD45R + CD27 + memory B cells measured by flow cytometry in controls (n=5), hiCNI (n=6) and loCNI (n=4). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05.
    Figure Legend Snippet: Activated T follicular helper cells drive GC formation. (A) immunofluorescence staining of a splenic follicle with T cell zone (CD3 + , red), GC (Ki67 + , circled green), B cell follicle (CD20 + , yellow) with mantle zone (MZ, circled yellow) and Tfh (white arrows). (B) average number of activated Tfh (CD3 + cells in Ki67 + GC area) and resting Tfh (CD3 + cells in CD20 + MZ area) in controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) Correlation of the number of activated Tfh and GC expansion within the loCNI group. (D) plasma cells measured as IgG + cells in spleen sections from controls (n=6), hiCNI (n=6) and loCNI (n=8), and (E) CD45R + CD27 + memory B cells measured by flow cytometry in controls (n=5), hiCNI (n=6) and loCNI (n=4). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05.

    Techniques Used: Immunofluorescence, Staining, Flow Cytometry

    Frequency and absolute numbers of leukocyte subsets in spleens and allografts. Representative flow cytometry data (A) , frequency and absolute numbers of B cells (CD45R + ), T cells (CD3 + ) and mononuclear phagocytes (MP, CD11b/c + ) in spleen (B) and allografts (C) measured by flow cytometry and shown as percentages of leukocytes or absolute numbers per weight. Means and individual data points are shown from controls (LEW spleen n=9, BN kidneys n=6), hiCNI (n=6) and loCNI (n=8); statistical significance between groups is shown as *p≤ 0.05, **p<0.01 and ***p<0.001.
    Figure Legend Snippet: Frequency and absolute numbers of leukocyte subsets in spleens and allografts. Representative flow cytometry data (A) , frequency and absolute numbers of B cells (CD45R + ), T cells (CD3 + ) and mononuclear phagocytes (MP, CD11b/c + ) in spleen (B) and allografts (C) measured by flow cytometry and shown as percentages of leukocytes or absolute numbers per weight. Means and individual data points are shown from controls (LEW spleen n=9, BN kidneys n=6), hiCNI (n=6) and loCNI (n=8); statistical significance between groups is shown as *p≤ 0.05, **p<0.01 and ***p<0.001.

    Techniques Used: Flow Cytometry

    Germinal center formation. (A) immunofluorescence staining of splenic follicles with T cell zone (CD3 + , red), B cell follicle (CD20 + , yellow) and GC (Ki67 + , green) and (B) average frequency and area of GC per follicle from controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) splenic AID mRNA expression measured by qPCR in controls (n=5), hiCNI (n=6) and loCNI (n=8), normalized to house-keeping gene HPRT and expressed as delta CT (AU). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05 and **p<0.01. (D) Correlation of GC frequency or area with IgG and IgG2b DSA MFI within the loCNI group.
    Figure Legend Snippet: Germinal center formation. (A) immunofluorescence staining of splenic follicles with T cell zone (CD3 + , red), B cell follicle (CD20 + , yellow) and GC (Ki67 + , green) and (B) average frequency and area of GC per follicle from controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) splenic AID mRNA expression measured by qPCR in controls (n=5), hiCNI (n=6) and loCNI (n=8), normalized to house-keeping gene HPRT and expressed as delta CT (AU). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05 and **p<0.01. (D) Correlation of GC frequency or area with IgG and IgG2b DSA MFI within the loCNI group.

    Techniques Used: Immunofluorescence, Staining, Expressing

    rat anti mouse cd3 apc ef780  (Thermo Fisher)


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    Thermo Fisher rat anti mouse cd3 apc ef780
    Increased myocardial infiltration of dendritic cells (DCs) in the right ventricle (RV) of Tnfaip3 DNGR1-KO mice. ( a ) Flow cytometry analysis for CD45+ cells (alive CD45 + cells), DCs <t>(CD3-/CD19-,</t> MHC-II+CD11c+), T cells <t>(CD3+)</t> and B cells (CD19+) in separately measured right and left ventricle cell suspensions from hearts of Tnfaip3 DNGR1-WT/KO mice; ( b ) mRNA expression (relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene) of DC markers CD11c and BATF3, and the T cell subset markers CD4 and CD8 in 24- and 31-week-old Tnfaip3 DNGR1-KO and wild-type (WT) control mice; ( c ) whole mount analysis of right ventricle staining for YFP (green) and MHCII (red) in age 14-, 24- or 31-week-old Tnfaip3 DNGR1-KO mice. Results are presented as mean values + standard deviations of 4–6 mice per group. * p < 0.05, ** p < 0.01; ( d ) Elastin van Gieson (EvG)-stained whole heart section histology of Tnfaip3 DNGR1-WT and Tnfaip3 DNGR1-KO mice for representative sections. Scale in left panels is 2.5 mm and in right panels 100 µm. Arrows indicate lymphocytes.
    Rat Anti Mouse Cd3 Apc Ef780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti mouse cd3 apc ef780/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat anti mouse cd3 apc ef780 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice"

    Article Title: Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22041756

    Increased myocardial infiltration of dendritic cells (DCs) in the right ventricle (RV) of Tnfaip3 DNGR1-KO mice. ( a ) Flow cytometry analysis for CD45+ cells (alive CD45 + cells), DCs (CD3-/CD19-, MHC-II+CD11c+), T cells (CD3+) and B cells (CD19+) in separately measured right and left ventricle cell suspensions from hearts of Tnfaip3 DNGR1-WT/KO mice; ( b ) mRNA expression (relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene) of DC markers CD11c and BATF3, and the T cell subset markers CD4 and CD8 in 24- and 31-week-old Tnfaip3 DNGR1-KO and wild-type (WT) control mice; ( c ) whole mount analysis of right ventricle staining for YFP (green) and MHCII (red) in age 14-, 24- or 31-week-old Tnfaip3 DNGR1-KO mice. Results are presented as mean values + standard deviations of 4–6 mice per group. * p < 0.05, ** p < 0.01; ( d ) Elastin van Gieson (EvG)-stained whole heart section histology of Tnfaip3 DNGR1-WT and Tnfaip3 DNGR1-KO mice for representative sections. Scale in left panels is 2.5 mm and in right panels 100 µm. Arrows indicate lymphocytes.
    Figure Legend Snippet: Increased myocardial infiltration of dendritic cells (DCs) in the right ventricle (RV) of Tnfaip3 DNGR1-KO mice. ( a ) Flow cytometry analysis for CD45+ cells (alive CD45 + cells), DCs (CD3-/CD19-, MHC-II+CD11c+), T cells (CD3+) and B cells (CD19+) in separately measured right and left ventricle cell suspensions from hearts of Tnfaip3 DNGR1-WT/KO mice; ( b ) mRNA expression (relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene) of DC markers CD11c and BATF3, and the T cell subset markers CD4 and CD8 in 24- and 31-week-old Tnfaip3 DNGR1-KO and wild-type (WT) control mice; ( c ) whole mount analysis of right ventricle staining for YFP (green) and MHCII (red) in age 14-, 24- or 31-week-old Tnfaip3 DNGR1-KO mice. Results are presented as mean values + standard deviations of 4–6 mice per group. * p < 0.05, ** p < 0.01; ( d ) Elastin van Gieson (EvG)-stained whole heart section histology of Tnfaip3 DNGR1-WT and Tnfaip3 DNGR1-KO mice for representative sections. Scale in left panels is 2.5 mm and in right panels 100 µm. Arrows indicate lymphocytes.

    Techniques Used: Flow Cytometry, Expressing, Staining

    Concomitant Bmpr2 mutation does not alter the pulmonary hypertension (PH) phenotype in Tnfaip3 DNGR1-KO mice. ( a ) Right ventricular systolic pressure (RVSP), determined by right heart catheterization and hypertrophy of RV measured by Fulton index (right ventricle/ left ventricle + septum) of the indicated mouse groups; ( b ) quantification of lung CD103+CD11c+ cDC1 frequencies and CD86 expression (MFI = median fluorescence intensity) by flow cytometry; ( c – f ) quantification of CD64+GR1- monocyte/macrophage population ( c ), CD3+ T cells ( d ), memory CD44+CD62L- CD8+ T cells ( e ) or CD19+ B cells ( f ) determined by flow cytometry in the lungs of the indicated mouse groups. Results are presented as mean + standard deviation of 10–31 mice ( a ) or 4–12 mice ( b – e ) per group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: Concomitant Bmpr2 mutation does not alter the pulmonary hypertension (PH) phenotype in Tnfaip3 DNGR1-KO mice. ( a ) Right ventricular systolic pressure (RVSP), determined by right heart catheterization and hypertrophy of RV measured by Fulton index (right ventricle/ left ventricle + septum) of the indicated mouse groups; ( b ) quantification of lung CD103+CD11c+ cDC1 frequencies and CD86 expression (MFI = median fluorescence intensity) by flow cytometry; ( c – f ) quantification of CD64+GR1- monocyte/macrophage population ( c ), CD3+ T cells ( d ), memory CD44+CD62L- CD8+ T cells ( e ) or CD19+ B cells ( f ) determined by flow cytometry in the lungs of the indicated mouse groups. Results are presented as mean + standard deviation of 10–31 mice ( a ) or 4–12 mice ( b – e ) per group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Mutagenesis, Expressing, Fluorescence, Flow Cytometry, Standard Deviation

    DCs and T cells are more often in close proximity around vessels and in parenchyma in Tnfaip3 DNGR1-KO mice. ( a ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old WT mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( b ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old Tnfaip3 DNGR1-KO mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( c ) DC (CD11c, red) and CD8+ T cells (CD8, blue) of WT (top) and Tnfaip3 DNGR1-KO (bottom) mice around a vessel. Magnification of selected area (right). Data shown are representative for 4–6 mice per group. Scale in left panels is 50 µm and of right panels 25 µm. Blue arrows indicate T-cells, and red arrows indicate DCs in the larger magnification (right panels).
    Figure Legend Snippet: DCs and T cells are more often in close proximity around vessels and in parenchyma in Tnfaip3 DNGR1-KO mice. ( a ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old WT mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( b ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old Tnfaip3 DNGR1-KO mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( c ) DC (CD11c, red) and CD8+ T cells (CD8, blue) of WT (top) and Tnfaip3 DNGR1-KO (bottom) mice around a vessel. Magnification of selected area (right). Data shown are representative for 4–6 mice per group. Scale in left panels is 50 µm and of right panels 25 µm. Blue arrows indicate T-cells, and red arrows indicate DCs in the larger magnification (right panels).

    Techniques Used: Staining

    mouse anti rat cd3 apc  (Thermo Fisher)


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    Thermo Fisher mouse anti rat cd3 apc
    Mouse Anti Rat Cd3 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti rat cd3 apc/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti rat cd3 apc - by Bioz Stars, 2024-04
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    Thermo Fisher apc efluor 780 rat monoclonal anti mouse cd3
    KEY RESOURCES TABLE
    Apc Efluor 780 Rat Monoclonal Anti Mouse Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc efluor 780 rat monoclonal anti mouse cd3/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apc efluor 780 rat monoclonal anti mouse cd3 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher mouse anti rat cd3 apc
    Activated T follicular helper cells drive GC formation. (A) immunofluorescence staining of a splenic follicle with T cell zone <t>(CD3</t> + , red), GC (Ki67 + , circled green), B cell follicle (CD20 + , yellow) with mantle zone (MZ, circled yellow) and Tfh (white arrows). (B) average number of activated Tfh <t>(CD3</t> + cells in Ki67 + GC area) and resting Tfh (CD3 + cells in CD20 + MZ area) in controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) Correlation of the number of activated Tfh and GC expansion within the loCNI group. (D) plasma cells measured as IgG + cells in spleen sections from controls (n=6), hiCNI (n=6) and loCNI (n=8), and (E) CD45R + CD27 + memory B cells measured by flow cytometry in controls (n=5), hiCNI (n=6) and loCNI (n=4). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05.
    Mouse Anti Rat Cd3 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti rat cd3 apc/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti rat cd3 apc - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher rat anti mouse cd3 apc ef780
    Increased myocardial infiltration of dendritic cells (DCs) in the right ventricle (RV) of Tnfaip3 DNGR1-KO mice. ( a ) Flow cytometry analysis for CD45+ cells (alive CD45 + cells), DCs <t>(CD3-/CD19-,</t> MHC-II+CD11c+), T cells <t>(CD3+)</t> and B cells (CD19+) in separately measured right and left ventricle cell suspensions from hearts of Tnfaip3 DNGR1-WT/KO mice; ( b ) mRNA expression (relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene) of DC markers CD11c and BATF3, and the T cell subset markers CD4 and CD8 in 24- and 31-week-old Tnfaip3 DNGR1-KO and wild-type (WT) control mice; ( c ) whole mount analysis of right ventricle staining for YFP (green) and MHCII (red) in age 14-, 24- or 31-week-old Tnfaip3 DNGR1-KO mice. Results are presented as mean values + standard deviations of 4–6 mice per group. * p < 0.05, ** p < 0.01; ( d ) Elastin van Gieson (EvG)-stained whole heart section histology of Tnfaip3 DNGR1-WT and Tnfaip3 DNGR1-KO mice for representative sections. Scale in left panels is 2.5 mm and in right panels 100 µm. Arrows indicate lymphocytes.
    Rat Anti Mouse Cd3 Apc Ef780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti mouse cd3 apc ef780/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat anti mouse cd3 apc ef780 - by Bioz Stars, 2024-04
    86/100 stars
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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Type I interferon decreases macrophage energy metabolism during mycobacterial infection

    doi: 10.1016/j.celrep.2021.109195

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: APC-eFluor 780 rat monoclonal anti-mouse CD3 (clone 17A2) , Thermo Fisher Scientific , Cat# 47-0032-82; RRID:AB_1272181.

    Techniques: Functional Assay, Recombinant, Electron Microscopy, Protease Inhibitor, CyQUANT Assay, LDH Cytotoxicity Assay, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Staining, RNA Sequencing Assay, Software

    Activated T follicular helper cells drive GC formation. (A) immunofluorescence staining of a splenic follicle with T cell zone (CD3 + , red), GC (Ki67 + , circled green), B cell follicle (CD20 + , yellow) with mantle zone (MZ, circled yellow) and Tfh (white arrows). (B) average number of activated Tfh (CD3 + cells in Ki67 + GC area) and resting Tfh (CD3 + cells in CD20 + MZ area) in controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) Correlation of the number of activated Tfh and GC expansion within the loCNI group. (D) plasma cells measured as IgG + cells in spleen sections from controls (n=6), hiCNI (n=6) and loCNI (n=8), and (E) CD45R + CD27 + memory B cells measured by flow cytometry in controls (n=5), hiCNI (n=6) and loCNI (n=4). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05.

    Journal: Frontiers in Immunology

    Article Title: Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose

    doi: 10.3389/fimmu.2021.657894

    Figure Lengend Snippet: Activated T follicular helper cells drive GC formation. (A) immunofluorescence staining of a splenic follicle with T cell zone (CD3 + , red), GC (Ki67 + , circled green), B cell follicle (CD20 + , yellow) with mantle zone (MZ, circled yellow) and Tfh (white arrows). (B) average number of activated Tfh (CD3 + cells in Ki67 + GC area) and resting Tfh (CD3 + cells in CD20 + MZ area) in controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) Correlation of the number of activated Tfh and GC expansion within the loCNI group. (D) plasma cells measured as IgG + cells in spleen sections from controls (n=6), hiCNI (n=6) and loCNI (n=8), and (E) CD45R + CD27 + memory B cells measured by flow cytometry in controls (n=5), hiCNI (n=6) and loCNI (n=4). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05.

    Article Snippet: Cells were blocked using 10% BSA PBS and stained with mouse anti-rat CD45R-PE-Cy7 (ThermoFisher 25-0460), mouse anti-rat CD11b/c-PE (ThermoFisher 12-0110-82), mouse anti-rat CD3-APC (ThermoFisher 17-0030-82) and hamster anti-mouse CD27-biotin (Serotec/Biorad MCA-4701B, Puchheim, Germany) and Streptavidin-APC (BD, 554067).

    Techniques: Immunofluorescence, Staining, Flow Cytometry

    Frequency and absolute numbers of leukocyte subsets in spleens and allografts. Representative flow cytometry data (A) , frequency and absolute numbers of B cells (CD45R + ), T cells (CD3 + ) and mononuclear phagocytes (MP, CD11b/c + ) in spleen (B) and allografts (C) measured by flow cytometry and shown as percentages of leukocytes or absolute numbers per weight. Means and individual data points are shown from controls (LEW spleen n=9, BN kidneys n=6), hiCNI (n=6) and loCNI (n=8); statistical significance between groups is shown as *p≤ 0.05, **p<0.01 and ***p<0.001.

    Journal: Frontiers in Immunology

    Article Title: Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose

    doi: 10.3389/fimmu.2021.657894

    Figure Lengend Snippet: Frequency and absolute numbers of leukocyte subsets in spleens and allografts. Representative flow cytometry data (A) , frequency and absolute numbers of B cells (CD45R + ), T cells (CD3 + ) and mononuclear phagocytes (MP, CD11b/c + ) in spleen (B) and allografts (C) measured by flow cytometry and shown as percentages of leukocytes or absolute numbers per weight. Means and individual data points are shown from controls (LEW spleen n=9, BN kidneys n=6), hiCNI (n=6) and loCNI (n=8); statistical significance between groups is shown as *p≤ 0.05, **p<0.01 and ***p<0.001.

    Article Snippet: Cells were blocked using 10% BSA PBS and stained with mouse anti-rat CD45R-PE-Cy7 (ThermoFisher 25-0460), mouse anti-rat CD11b/c-PE (ThermoFisher 12-0110-82), mouse anti-rat CD3-APC (ThermoFisher 17-0030-82) and hamster anti-mouse CD27-biotin (Serotec/Biorad MCA-4701B, Puchheim, Germany) and Streptavidin-APC (BD, 554067).

    Techniques: Flow Cytometry

    Germinal center formation. (A) immunofluorescence staining of splenic follicles with T cell zone (CD3 + , red), B cell follicle (CD20 + , yellow) and GC (Ki67 + , green) and (B) average frequency and area of GC per follicle from controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) splenic AID mRNA expression measured by qPCR in controls (n=5), hiCNI (n=6) and loCNI (n=8), normalized to house-keeping gene HPRT and expressed as delta CT (AU). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05 and **p<0.01. (D) Correlation of GC frequency or area with IgG and IgG2b DSA MFI within the loCNI group.

    Journal: Frontiers in Immunology

    Article Title: Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose

    doi: 10.3389/fimmu.2021.657894

    Figure Lengend Snippet: Germinal center formation. (A) immunofluorescence staining of splenic follicles with T cell zone (CD3 + , red), B cell follicle (CD20 + , yellow) and GC (Ki67 + , green) and (B) average frequency and area of GC per follicle from controls (n=6), hiCNI (n=6) and loCNI (n=8). (C) splenic AID mRNA expression measured by qPCR in controls (n=5), hiCNI (n=6) and loCNI (n=8), normalized to house-keeping gene HPRT and expressed as delta CT (AU). Means and individual data points are shown; statistical significance between groups is shown as *p≤ 0.05 and **p<0.01. (D) Correlation of GC frequency or area with IgG and IgG2b DSA MFI within the loCNI group.

    Article Snippet: Cells were blocked using 10% BSA PBS and stained with mouse anti-rat CD45R-PE-Cy7 (ThermoFisher 25-0460), mouse anti-rat CD11b/c-PE (ThermoFisher 12-0110-82), mouse anti-rat CD3-APC (ThermoFisher 17-0030-82) and hamster anti-mouse CD27-biotin (Serotec/Biorad MCA-4701B, Puchheim, Germany) and Streptavidin-APC (BD, 554067).

    Techniques: Immunofluorescence, Staining, Expressing

    Increased myocardial infiltration of dendritic cells (DCs) in the right ventricle (RV) of Tnfaip3 DNGR1-KO mice. ( a ) Flow cytometry analysis for CD45+ cells (alive CD45 + cells), DCs (CD3-/CD19-, MHC-II+CD11c+), T cells (CD3+) and B cells (CD19+) in separately measured right and left ventricle cell suspensions from hearts of Tnfaip3 DNGR1-WT/KO mice; ( b ) mRNA expression (relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene) of DC markers CD11c and BATF3, and the T cell subset markers CD4 and CD8 in 24- and 31-week-old Tnfaip3 DNGR1-KO and wild-type (WT) control mice; ( c ) whole mount analysis of right ventricle staining for YFP (green) and MHCII (red) in age 14-, 24- or 31-week-old Tnfaip3 DNGR1-KO mice. Results are presented as mean values + standard deviations of 4–6 mice per group. * p < 0.05, ** p < 0.01; ( d ) Elastin van Gieson (EvG)-stained whole heart section histology of Tnfaip3 DNGR1-WT and Tnfaip3 DNGR1-KO mice for representative sections. Scale in left panels is 2.5 mm and in right panels 100 µm. Arrows indicate lymphocytes.

    Journal: International Journal of Molecular Sciences

    Article Title: Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice

    doi: 10.3390/ijms22041756

    Figure Lengend Snippet: Increased myocardial infiltration of dendritic cells (DCs) in the right ventricle (RV) of Tnfaip3 DNGR1-KO mice. ( a ) Flow cytometry analysis for CD45+ cells (alive CD45 + cells), DCs (CD3-/CD19-, MHC-II+CD11c+), T cells (CD3+) and B cells (CD19+) in separately measured right and left ventricle cell suspensions from hearts of Tnfaip3 DNGR1-WT/KO mice; ( b ) mRNA expression (relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene) of DC markers CD11c and BATF3, and the T cell subset markers CD4 and CD8 in 24- and 31-week-old Tnfaip3 DNGR1-KO and wild-type (WT) control mice; ( c ) whole mount analysis of right ventricle staining for YFP (green) and MHCII (red) in age 14-, 24- or 31-week-old Tnfaip3 DNGR1-KO mice. Results are presented as mean values + standard deviations of 4–6 mice per group. * p < 0.05, ** p < 0.01; ( d ) Elastin van Gieson (EvG)-stained whole heart section histology of Tnfaip3 DNGR1-WT and Tnfaip3 DNGR1-KO mice for representative sections. Scale in left panels is 2.5 mm and in right panels 100 µm. Arrows indicate lymphocytes.

    Article Snippet: To determine co-localization of CD11c with either CD3+ or CD8+ T cells, lung slides were first stained with hamster anti-mouse CD11c PE (clone N418, Thermo Fisher Scientific) and rat anti-mouse CD3 APC-ef780 (clone 17A2, Thermo Fisher Scientific), or rat anti-mouse CD8 Fitc (clone 53-6.7, BD Bioscience, San Jose, CA, USA).

    Techniques: Flow Cytometry, Expressing, Staining

    Concomitant Bmpr2 mutation does not alter the pulmonary hypertension (PH) phenotype in Tnfaip3 DNGR1-KO mice. ( a ) Right ventricular systolic pressure (RVSP), determined by right heart catheterization and hypertrophy of RV measured by Fulton index (right ventricle/ left ventricle + septum) of the indicated mouse groups; ( b ) quantification of lung CD103+CD11c+ cDC1 frequencies and CD86 expression (MFI = median fluorescence intensity) by flow cytometry; ( c – f ) quantification of CD64+GR1- monocyte/macrophage population ( c ), CD3+ T cells ( d ), memory CD44+CD62L- CD8+ T cells ( e ) or CD19+ B cells ( f ) determined by flow cytometry in the lungs of the indicated mouse groups. Results are presented as mean + standard deviation of 10–31 mice ( a ) or 4–12 mice ( b – e ) per group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice

    doi: 10.3390/ijms22041756

    Figure Lengend Snippet: Concomitant Bmpr2 mutation does not alter the pulmonary hypertension (PH) phenotype in Tnfaip3 DNGR1-KO mice. ( a ) Right ventricular systolic pressure (RVSP), determined by right heart catheterization and hypertrophy of RV measured by Fulton index (right ventricle/ left ventricle + septum) of the indicated mouse groups; ( b ) quantification of lung CD103+CD11c+ cDC1 frequencies and CD86 expression (MFI = median fluorescence intensity) by flow cytometry; ( c – f ) quantification of CD64+GR1- monocyte/macrophage population ( c ), CD3+ T cells ( d ), memory CD44+CD62L- CD8+ T cells ( e ) or CD19+ B cells ( f ) determined by flow cytometry in the lungs of the indicated mouse groups. Results are presented as mean + standard deviation of 10–31 mice ( a ) or 4–12 mice ( b – e ) per group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: To determine co-localization of CD11c with either CD3+ or CD8+ T cells, lung slides were first stained with hamster anti-mouse CD11c PE (clone N418, Thermo Fisher Scientific) and rat anti-mouse CD3 APC-ef780 (clone 17A2, Thermo Fisher Scientific), or rat anti-mouse CD8 Fitc (clone 53-6.7, BD Bioscience, San Jose, CA, USA).

    Techniques: Mutagenesis, Expressing, Fluorescence, Flow Cytometry, Standard Deviation

    DCs and T cells are more often in close proximity around vessels and in parenchyma in Tnfaip3 DNGR1-KO mice. ( a ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old WT mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( b ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old Tnfaip3 DNGR1-KO mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( c ) DC (CD11c, red) and CD8+ T cells (CD8, blue) of WT (top) and Tnfaip3 DNGR1-KO (bottom) mice around a vessel. Magnification of selected area (right). Data shown are representative for 4–6 mice per group. Scale in left panels is 50 µm and of right panels 25 µm. Blue arrows indicate T-cells, and red arrows indicate DCs in the larger magnification (right panels).

    Journal: International Journal of Molecular Sciences

    Article Title: Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice

    doi: 10.3390/ijms22041756

    Figure Lengend Snippet: DCs and T cells are more often in close proximity around vessels and in parenchyma in Tnfaip3 DNGR1-KO mice. ( a ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old WT mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( b ) DC (CD11c, red) and T cell (CD3, blue) staining on lung cryosections of 31-week-old Tnfaip3 DNGR1-KO mice of which an area around a vessel (top) and parenchyma (bottom) of the same mouse is depicted. Magnification of selected areas (right); ( c ) DC (CD11c, red) and CD8+ T cells (CD8, blue) of WT (top) and Tnfaip3 DNGR1-KO (bottom) mice around a vessel. Magnification of selected area (right). Data shown are representative for 4–6 mice per group. Scale in left panels is 50 µm and of right panels 25 µm. Blue arrows indicate T-cells, and red arrows indicate DCs in the larger magnification (right panels).

    Article Snippet: To determine co-localization of CD11c with either CD3+ or CD8+ T cells, lung slides were first stained with hamster anti-mouse CD11c PE (clone N418, Thermo Fisher Scientific) and rat anti-mouse CD3 APC-ef780 (clone 17A2, Thermo Fisher Scientific), or rat anti-mouse CD8 Fitc (clone 53-6.7, BD Bioscience, San Jose, CA, USA).

    Techniques: Staining