mouse anti ph2ax  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc mouse anti ph2ax
    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, <t>pH2AX,</t> p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Mouse Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors"

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    Journal: iScience

    doi: 10.1016/j.isci.2023.108169

    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Figure Legend Snippet: Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Techniques Used: Staining

    Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.
    Figure Legend Snippet: Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Techniques Used: Staining, Marker

    Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.
    Figure Legend Snippet: Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Techniques Used: Expressing, Activity Assay, Immunofluorescence, Staining, Fluorescence


    Figure Legend Snippet:

    Techniques Used: Recombinant, Bicinchoninic Acid Protein Assay, Software

    mouse anti ph2ax  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc mouse anti ph2ax
    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, <t>pH2AX,</t> p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Mouse Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors"

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    Journal: iScience

    doi: 10.1016/j.isci.2023.108169

    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Figure Legend Snippet: Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Techniques Used: Staining

    Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.
    Figure Legend Snippet: Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Techniques Used: Staining, Marker

    Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.
    Figure Legend Snippet: Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Techniques Used: Expressing, Activity Assay, Immunofluorescence, Staining, Fluorescence


    Figure Legend Snippet:

    Techniques Used: Recombinant, Bicinchoninic Acid Protein Assay, Software

    mouse anti ph2ax ser139  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc mouse anti ph2ax ser139
    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, <t>pH2AX,</t> p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Mouse Anti Ph2ax Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax ser139 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors"

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    Journal: iScience

    doi: 10.1016/j.isci.2023.108169

    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Figure Legend Snippet: Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Techniques Used: Staining

    Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.
    Figure Legend Snippet: Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Techniques Used: Staining, Marker

    Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.
    Figure Legend Snippet: Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Techniques Used: Expressing, Activity Assay, Immunofluorescence, Staining, Fluorescence


    Figure Legend Snippet:

    Techniques Used: Recombinant, Bicinchoninic Acid Protein Assay, Software

    mouse anti ph2ax  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc mouse anti ph2ax
    A. Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers-53BP1, H2AX, <t>pH2AX</t> in red; Cell cycle regulators-p53, p16, p21 in purple. B. Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM= Premalignant gastric lesion, M= Malignant gastric lesion. C. Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image.
    Mouse Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Replicative stress in gastroesophageal adenocarcinoma is associated with chromosomal instability and sensitivity to DNA damage response inhibitors"

    Article Title: Replicative stress in gastroesophageal adenocarcinoma is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    Journal: bioRxiv

    doi: 10.1101/2023.03.27.534412

    A. Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers-53BP1, H2AX, pH2AX in red; Cell cycle regulators-p53, p16, p21 in purple. B. Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM= Premalignant gastric lesion, M= Malignant gastric lesion. C. Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image.
    Figure Legend Snippet: A. Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers-53BP1, H2AX, pH2AX in red; Cell cycle regulators-p53, p16, p21 in purple. B. Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM= Premalignant gastric lesion, M= Malignant gastric lesion. C. Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image.

    Techniques Used: Staining

    A. Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (grey), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. B. Representative IHC images of the staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5).
    Figure Legend Snippet: A. Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (grey), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. B. Representative IHC images of the staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5).

    Techniques Used: Staining, Marker

    A. Correlation plot between H2AX gene expression (log2(TPM+1)) and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines; R squared value and p-value calculated by simple linear correlation analysis. B. H2AX expression (log2(TPM+1)) between ‘No WGD (0)’ (grey) and ‘with WGD (1/2)’ (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; Difference between the H2AX expression is represented as the mean±S.D.; P-value calculated by unpaired t-test. C. Ploidy abnormalities scores for the five gastric cancer cell lines. D. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); Nucleus stained with DAPI (blue). E. Quantification of data in D., expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells-RPE1-718, AGS-2080, HGC27, 1031, NUGC3-2119, GSU-2092, KE39-577. F. Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. G. Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. H. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic ‘low-replication stress’-RPE1, AGS, and intrinsic ‘high-replication stress’-GSU, KE39.
    Figure Legend Snippet: A. Correlation plot between H2AX gene expression (log2(TPM+1)) and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines; R squared value and p-value calculated by simple linear correlation analysis. B. H2AX expression (log2(TPM+1)) between ‘No WGD (0)’ (grey) and ‘with WGD (1/2)’ (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; Difference between the H2AX expression is represented as the mean±S.D.; P-value calculated by unpaired t-test. C. Ploidy abnormalities scores for the five gastric cancer cell lines. D. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); Nucleus stained with DAPI (blue). E. Quantification of data in D., expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells-RPE1-718, AGS-2080, HGC27, 1031, NUGC3-2119, GSU-2092, KE39-577. F. Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. G. Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. H. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic ‘low-replication stress’-RPE1, AGS, and intrinsic ‘high-replication stress’-GSU, KE39.

    Techniques Used: Expressing, Immunofluorescence, Staining, Fluorescence

    mouse anti ph2ax  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc mouse anti ph2ax
    A. Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers-53BP1, H2AX, <t>pH2AX</t> in red; Cell cycle regulators-p53, p16, p21 in purple. B. Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM= Premalignant gastric lesion, M= Malignant gastric lesion. C. Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image.
    Mouse Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Replicative stress in gastroesophageal adenocarcinoma is associated with chromosomal instability and sensitivity to DNA damage response inhibitors"

    Article Title: Replicative stress in gastroesophageal adenocarcinoma is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    Journal: bioRxiv

    doi: 10.1101/2023.03.27.534412

    A. Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers-53BP1, H2AX, pH2AX in red; Cell cycle regulators-p53, p16, p21 in purple. B. Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM= Premalignant gastric lesion, M= Malignant gastric lesion. C. Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image.
    Figure Legend Snippet: A. Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers-53BP1, H2AX, pH2AX in red; Cell cycle regulators-p53, p16, p21 in purple. B. Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM= Premalignant gastric lesion, M= Malignant gastric lesion. C. Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image.

    Techniques Used: Staining

    A. Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (grey), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. B. Representative IHC images of the staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5).
    Figure Legend Snippet: A. Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (grey), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. B. Representative IHC images of the staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5).

    Techniques Used: Staining, Marker

    A. Correlation plot between H2AX gene expression (log2(TPM+1)) and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines; R squared value and p-value calculated by simple linear correlation analysis. B. H2AX expression (log2(TPM+1)) between ‘No WGD (0)’ (grey) and ‘with WGD (1/2)’ (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; Difference between the H2AX expression is represented as the mean±S.D.; P-value calculated by unpaired t-test. C. Ploidy abnormalities scores for the five gastric cancer cell lines. D. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); Nucleus stained with DAPI (blue). E. Quantification of data in D., expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells-RPE1-718, AGS-2080, HGC27, 1031, NUGC3-2119, GSU-2092, KE39-577. F. Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. G. Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. H. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic ‘low-replication stress’-RPE1, AGS, and intrinsic ‘high-replication stress’-GSU, KE39.
    Figure Legend Snippet: A. Correlation plot between H2AX gene expression (log2(TPM+1)) and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines; R squared value and p-value calculated by simple linear correlation analysis. B. H2AX expression (log2(TPM+1)) between ‘No WGD (0)’ (grey) and ‘with WGD (1/2)’ (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; Difference between the H2AX expression is represented as the mean±S.D.; P-value calculated by unpaired t-test. C. Ploidy abnormalities scores for the five gastric cancer cell lines. D. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); Nucleus stained with DAPI (blue). E. Quantification of data in D., expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells-RPE1-718, AGS-2080, HGC27, 1031, NUGC3-2119, GSU-2092, KE39-577. F. Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. G. Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with ‘intrinsic low-replication stress’-RPE1, AGS (grey) and ‘intrinsic high-replication stress’-GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ± S.D from the following number of cells-RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM-350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM-1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. H. Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic ‘low-replication stress’-RPE1, AGS, and intrinsic ‘high-replication stress’-GSU, KE39.

    Techniques Used: Expressing, Immunofluorescence, Staining, Fluorescence

    mouse specific ph2ax rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc mouse specific ph2ax rabbit mab
    Mouse Specific Ph2ax Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse specific ph2ax rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse specific ph2ax rabbit mab - by Bioz Stars, 2024-06
    86/100 stars

    Images

    mouse specific ph2ax rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc mouse specific ph2ax rabbit mab
    Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter <t>pH2AX</t> ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.
    Mouse Specific Ph2ax Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse specific ph2ax rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse specific ph2ax rabbit mab - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance"

    Article Title: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance

    Journal: Communications Chemistry

    doi: 10.1038/s42004-022-00661-z

    Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter pH2AX ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: Cancer proliferative activity ( a ) and GSH content ( b ) as well as DNA damage in tumor ( c ) and kidney ( d ) tissues. Mice bearing CT26 allografts ( n = 4 per group) were treated twice a week with equimolar concentrations of BSO-OxMal (23.5 mg/kg) or oxaliplatin (9 mg/kg) and sacrificed 24 h after the last drug dosing. Immunohistochemical staining of Ki-67 ( a ) and the DNA damage parameter pH2AX ( c ) were quantified in 8–10 regions of interest (ROI) per tumor within random non-necrotic, viable cancer regions by ImageJ software. In ( d ) cells stained positively for pH2AX in the kidney were counted within 5 ROI per kidney section. Representative images are shown for tumor sections in Supplementary Fig. and for organ sections in Supplementary Fig. . In ( b ) tumor GSH content of the respective cancer samples was quantified by a calorimetric method as described in the methods section. Data in ( b , d ) are depicted as mean ± SEM (standard error of mean). Statistical significance was tested using one-way ANOVA ( a , c ) or Student´s t test ( b , d ). In all cases: * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used: Activity Assay, Immunohistochemical staining, Staining, Software

    anti ph2ax  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti ph2ax
    a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and <t>pH2AX</t> immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.
    Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ph2ax/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ph2ax - by Bioz Stars, 2024-06
    94/100 stars

    Images

    1) Product Images from "Uropathogenic E. coli induces DNA damage in the bladder"

    Article Title: Uropathogenic E. coli induces DNA damage in the bladder

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1009310

    a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and pH2AX immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.
    Figure Legend Snippet: a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and pH2AX immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Electrophoresis, Molecular Weight, Immunofluorescence, Staining, Infection, Mutagenesis

    a-d . Confocal microscopic detection of ClbI-GFP expression and pH2AX in frozen bladder sections, 6 hours post infection with UTI89, hosting the p- clbI - gfp fusion plasmid. The individual channel images are shown in grayscale, the umbrella cell containing the IBC is circled with a yellow dashed line. In the merged (d) image: blue = DNA, green = ClbI-GFP, magenta = pH2AX, grey = phase contrast. The immunofluorescence staining was repeated three times on different slides, other representative images are shown in . e. Bladder tissue E . coli counts, 6 h and 24 h after infection with wild-type E . coli UTI89 (circles) or Δ clbP (squares). Each data point corresponds to one mouse (n = 6), with mean ± standard error of the mean (SEM) shown for each group. Mann-Whitney U test. f-l. Bladder sections 6 hours post inoculation with wild-type UTI89 (f, g) or the isogenic Δ clbP mutant (h-l) stained with haematoxylin-eosin (f,h,j), FISH (g,i,k) or DAPI (l, grayscale) all of which detect IBCs (arrows). In the FISH images: blue = DAPI stain DNA; green = FITC-conjugated wheat germ agglutinin (WGA) stained polysaccharides; red = bacterial cells, labelled with the universal 16S FISH probe. The images are representative of the 5 animals per group observed.
    Figure Legend Snippet: a-d . Confocal microscopic detection of ClbI-GFP expression and pH2AX in frozen bladder sections, 6 hours post infection with UTI89, hosting the p- clbI - gfp fusion plasmid. The individual channel images are shown in grayscale, the umbrella cell containing the IBC is circled with a yellow dashed line. In the merged (d) image: blue = DNA, green = ClbI-GFP, magenta = pH2AX, grey = phase contrast. The immunofluorescence staining was repeated three times on different slides, other representative images are shown in . e. Bladder tissue E . coli counts, 6 h and 24 h after infection with wild-type E . coli UTI89 (circles) or Δ clbP (squares). Each data point corresponds to one mouse (n = 6), with mean ± standard error of the mean (SEM) shown for each group. Mann-Whitney U test. f-l. Bladder sections 6 hours post inoculation with wild-type UTI89 (f, g) or the isogenic Δ clbP mutant (h-l) stained with haematoxylin-eosin (f,h,j), FISH (g,i,k) or DAPI (l, grayscale) all of which detect IBCs (arrows). In the FISH images: blue = DAPI stain DNA; green = FITC-conjugated wheat germ agglutinin (WGA) stained polysaccharides; red = bacterial cells, labelled with the universal 16S FISH probe. The images are representative of the 5 animals per group observed.

    Techniques Used: Expressing, Infection, Plasmid Preparation, Immunofluorescence, Staining, MANN-WHITNEY, Mutagenesis

    a-i. Immunofluorescence staining of pH2AX on paraffin-embedded bladder sections 24 hours after PBS inoculation (a-c), or UTI89 wild-type (d-f) or UTI89Δ clbP (g-i) infection. The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, grey = phase contrast. Yellow arrows highlight bacteria, white dotted lines represent the basal membrane of the urothelium. Scale bar = 10 μm. The experiment was repeated twice on 5 to 8 mice, other representative images are shown in . j-l. pH2AX positive nuclei (j), Krt14 positive cells (k) and Krt14 and pH2AX positive cells (l) were counted objectively using Fiji and CellProfiler softwares on the confocal images from large mosaic of bladder sections. Each dot represents one mouse bladder. Mann Whitney U-test performed between UTI89 wild-type and UTI89 Δ clbP . m-t. Paraffin-embedded bladders sections were immuno-stained for pH2AX and Krt14 24 hours post infection with UTI89 wild-type (m-p) or UTI89Δ clbP (q-t). The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, green = Krt14. Cells positive for both Krt14 and pH2AX are circled in pink. White dotted lines highlight the basal membrane of the urothelium. Scale bar = 10 μm. Other representative images are shown in .
    Figure Legend Snippet: a-i. Immunofluorescence staining of pH2AX on paraffin-embedded bladder sections 24 hours after PBS inoculation (a-c), or UTI89 wild-type (d-f) or UTI89Δ clbP (g-i) infection. The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, grey = phase contrast. Yellow arrows highlight bacteria, white dotted lines represent the basal membrane of the urothelium. Scale bar = 10 μm. The experiment was repeated twice on 5 to 8 mice, other representative images are shown in . j-l. pH2AX positive nuclei (j), Krt14 positive cells (k) and Krt14 and pH2AX positive cells (l) were counted objectively using Fiji and CellProfiler softwares on the confocal images from large mosaic of bladder sections. Each dot represents one mouse bladder. Mann Whitney U-test performed between UTI89 wild-type and UTI89 Δ clbP . m-t. Paraffin-embedded bladders sections were immuno-stained for pH2AX and Krt14 24 hours post infection with UTI89 wild-type (m-p) or UTI89Δ clbP (q-t). The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, green = Krt14. Cells positive for both Krt14 and pH2AX are circled in pink. White dotted lines highlight the basal membrane of the urothelium. Scale bar = 10 μm. Other representative images are shown in .

    Techniques Used: Immunofluorescence, Staining, Infection, Fluorescence, MANN-WHITNEY

    anti ph2ax ser139 mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti ph2ax ser139 mab
    Anti Ph2ax Ser139 Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ph2ax ser139 mab/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ph2ax ser139 mab - by Bioz Stars, 2024-06
    94/100 stars

    Images

    anti ph2ax ser139 mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti ph2ax ser139 mab
    Anti Ph2ax Ser139 Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ph2ax ser139 mab/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ph2ax ser139 mab - by Bioz Stars, 2024-06
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Cell Signaling Technology Inc mouse anti ph2ax
    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, <t>pH2AX,</t> p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Mouse Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc mouse anti ph2ax ser139
    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, <t>pH2AX,</t> p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Mouse Anti Ph2ax Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ph2ax ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ph2ax ser139 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc mouse specific ph2ax rabbit mab
    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, <t>pH2AX,</t> p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.
    Mouse Specific Ph2ax Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse specific ph2ax rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse specific ph2ax rabbit mab - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc anti ph2ax
    a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and <t>pH2AX</t> immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.
    Anti Ph2ax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ph2ax/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ph2ax - by Bioz Stars, 2024-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc anti ph2ax ser139 mab
    a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and <t>pH2AX</t> immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.
    Anti Ph2ax Ser139 Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ph2ax ser139 mab/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ph2ax ser139 mab - by Bioz Stars, 2024-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet: Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Article Snippet: Cells were then washed and fixed with 4% paraformaldehyde (PFA) for 15 minutes at room temperature, blocked and permeabilized PBS-BSA + 0.3% Triton X-100 for 15 minutes at room temperature, and incubated with primary antibodies (rabbit anti-pTIF1β/pKAP1 (Ser824) (1:500, CST # 4127S) and Mouse anti-pH2AX (1:600, CST #80312) in PBS-BSA overnight at +4°C.

    Techniques: Staining

    Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet: Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Article Snippet: Cells were then washed and fixed with 4% paraformaldehyde (PFA) for 15 minutes at room temperature, blocked and permeabilized PBS-BSA + 0.3% Triton X-100 for 15 minutes at room temperature, and incubated with primary antibodies (rabbit anti-pTIF1β/pKAP1 (Ser824) (1:500, CST # 4127S) and Mouse anti-pH2AX (1:600, CST #80312) in PBS-BSA overnight at +4°C.

    Techniques: Staining, Marker

    Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet: Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Article Snippet: Cells were then washed and fixed with 4% paraformaldehyde (PFA) for 15 minutes at room temperature, blocked and permeabilized PBS-BSA + 0.3% Triton X-100 for 15 minutes at room temperature, and incubated with primary antibodies (rabbit anti-pTIF1β/pKAP1 (Ser824) (1:500, CST # 4127S) and Mouse anti-pH2AX (1:600, CST #80312) in PBS-BSA overnight at +4°C.

    Techniques: Expressing, Activity Assay, Immunofluorescence, Staining, Fluorescence

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet:

    Article Snippet: Cells were then washed and fixed with 4% paraformaldehyde (PFA) for 15 minutes at room temperature, blocked and permeabilized PBS-BSA + 0.3% Triton X-100 for 15 minutes at room temperature, and incubated with primary antibodies (rabbit anti-pTIF1β/pKAP1 (Ser824) (1:500, CST # 4127S) and Mouse anti-pH2AX (1:600, CST #80312) in PBS-BSA overnight at +4°C.

    Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Software

    Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet: Evaluation of DDR and cell cycle markers in matched normal, premalignant, and malignant human gastric specimens (A) Tabulated summary of IHC stainings indicating the number of premalignant (green) and malignant (blue) gastric lesions scored for each staining criterion. DDR/replication stress markers- 53BP1, H2AX, pH2AX, p53 in red; Cell cycle regulators- p16, p21 in purple. (B) Summary of the stainings (DDR markers and the cell cycle regulation markers) in paired premalignant and malignant gastric lesions from gastric cancer patient8. PM = Premalignant gastric lesion, M = Malignant gastric lesion. (C) Representative IHC images of paired premalignant and malignant gastric lesions from a gastric cancer patient (patient8) stained for DDR markers and cell cycle regulation markers, including H&E staining. Insets represent the zoomed area for each image. Scale bar: 100 μM.

    Article Snippet: Mouse anti-pH2AX ser139 , Cell Signaling Technology , Cat# 80312; RRID: AB_2799949.

    Techniques: Staining

    Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet: Evaluation of DDR markers linked with dsDNA breaks and cell cycle regulation markers in the carcinogen-induced mouse model of gastric premalignancy (A) Summary of dysplasia grade and staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal (gray), premalignant (green), and malignant (blue) gastric lesions of four MNU treated mice. (B) Representative IHC images of staining pattern of proliferation marker ki67, DDR marker pH2AX, and cell cycle regulation markers (p53 and p21) in paired normal, premalignant, and malignant gastric lesions of MNU treated mouse4 (dysplasia score 5). Scale bar: 100 μM.

    Article Snippet: Mouse anti-pH2AX ser139 , Cell Signaling Technology , Cat# 80312; RRID: AB_2799949.

    Techniques: Staining, Marker

    Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet: Aneuploidy correlates and H2AX expression correlates with DDR pathway activity in gastric cancer (A) Correlation plot between aneuploidy score and CIN25 score (DNA damage checkpoint expression) for the CCLE gastric cancer cell lines. R squared value and p value calculated by simple linear correlation analysis. (B) H2AX expression (log2(TPM+1)) between “No WGD (0)” (gray) and “with WGD (1/2)” (green) groups of gastric cancer cell lines available in the BROAD institute PRISM repurposing drug screen dataset; difference between the H2AX expression is represented as the mean ± S.D.; p value calculated by unpaired t test. (C) Ploidy abnormalities scores and p53 status for the five gastric cancer cell lines. (D) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in untreated five gastric cancer cell lines and RPE1 cells (non-neoplastic cell line) representing intrinsic replication stress/double-stranded DNA breaks (DSBs); nucleus stained with DAPI (blue); Scale bar: 50 μm. (E) Quantification of pH2AX and pKAP1 in indicated cell lines expressed as mean fluorescence intensity per cell (MFI) ± S.D from the following number of cells- RPE1-718, AGS- 2080, HGC27, 1031, NUGC3- 2119, GSU- 2092, KE39- 577. (F) Quantification of pH2AX staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (red). Data expressed as mean fluorescence intensity (MFI) of pH2AX signal per cell ± S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (G) Quantification of pKAP1 staining in prexasertib (Chk1/2) dose-dependent induced replication stress in cell lines with “intrinsic low-replication stress”-RPE1, AGS (gray) and “intrinsic high-replication stress”- GSU, KE39 (green). Data expressed as mean fluorescence intensity of pKAP1 signal per cell ±S.D from the following number of cells- RPE1: Control-718, 0.2nM-888, 2.0nM-524, 20.0nM-570, 200.0nM- 350; AGS: Control-2080, 0.2nM-1487, 2.0nM-1996, 20.0nM-1211, 200.0nM- 1396; GSU: Control-2092, 0.2nM-3319, 2.0nM-4035, 20.0nM-2057, 200.0nM-2402; KE39: Control-577, 0.2nM-1004, 2.0nM-1022, 20.0nM-728, 200.0nM-958. (H) Representative immunofluorescence images of pH2AX (red) and pKAP1 (green) stainings in prexasertib (20nM) treated cell lines with intrinsic “low-replication stress”-RPE1, AGS, and intrinsic “high-replication stress”- GSU, KE39; Scale bar: 50μm.

    Article Snippet: Mouse anti-pH2AX ser139 , Cell Signaling Technology , Cat# 80312; RRID: AB_2799949.

    Techniques: Expressing, Activity Assay, Immunofluorescence, Staining, Fluorescence

    Journal: iScience

    Article Title: Replicative stress in gastroesophageal cancer is associated with chromosomal instability and sensitivity to DNA damage response inhibitors

    doi: 10.1016/j.isci.2023.108169

    Figure Lengend Snippet:

    Article Snippet: Mouse anti-pH2AX ser139 , Cell Signaling Technology , Cat# 80312; RRID: AB_2799949.

    Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Software

    a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and pH2AX immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.

    Journal: PLoS Pathogens

    Article Title: Uropathogenic E. coli induces DNA damage in the bladder

    doi: 10.1371/journal.ppat.1009310

    Figure Lengend Snippet: a . LC-MS/MS quantification of the C14-Asn cleavage product released following colibactin maturation by the ClbP peptidase in bacterial pellets of UPEC strains UTI89, CFT073 and their respective Δ clbN mutants (which are not able to synthesise C14-Asn), and four clinical UPEC strains S7, S238, S245, S257. Quantifications were performed in triplicate and represented as a mean ± standard error of the mean (SEM). nd: not detected b. Formation of DNA interstrand cross-links upon exposure of linear double stranded DNA to UTI89, NC101 and their respective Δ clbP mutants as well as seven representative clinical human UPEC isolates (S7, S45, S122, S228, S238, S254, S257), visualised by electrophoresis under denaturing conditions. DNA with interstrand cross-links (red arrow) migrates at an apparent molecular weight of twice that of DNA without cross-links. In the absence of any crosslinking DNA migrates as a single denatured strand. The gel shown is representative of three independent experiments. c. S33p-RPA32 and pH2AX immunofluorescence staining of HeLa cells, 16 hours after infection with the reference NC101 strain, its Δ clbP mutant or human UPEC strains S45, S122, S228. pH2AX and S33p-RPA32 = pRPA: grayscale. Merged: green = pH2AX; magenta = S33p-RPA32; blue = DAPI; scale bar = 10 μm. Human UPEC strains from AS = asymptomatic bacteriuria; IU = cystitis; P = pyelonephritis.

    Article Snippet: Slices were stained with anti-pH2AX (Rabbit, Cell Signalling S9718; or Mouse, Millipore #O5-636) at a dilution of 1:200, anti-Krt14 antibodies (Chicken, Ozyme BLE906001) at a dilution of 1:250 and anti-Upk III (Rabbit, Abcam, ab157801) at a dilution of 1:800 in MaxBlock, 0.3% Triton X100.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Electrophoresis, Molecular Weight, Immunofluorescence, Staining, Infection, Mutagenesis

    a-d . Confocal microscopic detection of ClbI-GFP expression and pH2AX in frozen bladder sections, 6 hours post infection with UTI89, hosting the p- clbI - gfp fusion plasmid. The individual channel images are shown in grayscale, the umbrella cell containing the IBC is circled with a yellow dashed line. In the merged (d) image: blue = DNA, green = ClbI-GFP, magenta = pH2AX, grey = phase contrast. The immunofluorescence staining was repeated three times on different slides, other representative images are shown in . e. Bladder tissue E . coli counts, 6 h and 24 h after infection with wild-type E . coli UTI89 (circles) or Δ clbP (squares). Each data point corresponds to one mouse (n = 6), with mean ± standard error of the mean (SEM) shown for each group. Mann-Whitney U test. f-l. Bladder sections 6 hours post inoculation with wild-type UTI89 (f, g) or the isogenic Δ clbP mutant (h-l) stained with haematoxylin-eosin (f,h,j), FISH (g,i,k) or DAPI (l, grayscale) all of which detect IBCs (arrows). In the FISH images: blue = DAPI stain DNA; green = FITC-conjugated wheat germ agglutinin (WGA) stained polysaccharides; red = bacterial cells, labelled with the universal 16S FISH probe. The images are representative of the 5 animals per group observed.

    Journal: PLoS Pathogens

    Article Title: Uropathogenic E. coli induces DNA damage in the bladder

    doi: 10.1371/journal.ppat.1009310

    Figure Lengend Snippet: a-d . Confocal microscopic detection of ClbI-GFP expression and pH2AX in frozen bladder sections, 6 hours post infection with UTI89, hosting the p- clbI - gfp fusion plasmid. The individual channel images are shown in grayscale, the umbrella cell containing the IBC is circled with a yellow dashed line. In the merged (d) image: blue = DNA, green = ClbI-GFP, magenta = pH2AX, grey = phase contrast. The immunofluorescence staining was repeated three times on different slides, other representative images are shown in . e. Bladder tissue E . coli counts, 6 h and 24 h after infection with wild-type E . coli UTI89 (circles) or Δ clbP (squares). Each data point corresponds to one mouse (n = 6), with mean ± standard error of the mean (SEM) shown for each group. Mann-Whitney U test. f-l. Bladder sections 6 hours post inoculation with wild-type UTI89 (f, g) or the isogenic Δ clbP mutant (h-l) stained with haematoxylin-eosin (f,h,j), FISH (g,i,k) or DAPI (l, grayscale) all of which detect IBCs (arrows). In the FISH images: blue = DAPI stain DNA; green = FITC-conjugated wheat germ agglutinin (WGA) stained polysaccharides; red = bacterial cells, labelled with the universal 16S FISH probe. The images are representative of the 5 animals per group observed.

    Article Snippet: Slices were stained with anti-pH2AX (Rabbit, Cell Signalling S9718; or Mouse, Millipore #O5-636) at a dilution of 1:200, anti-Krt14 antibodies (Chicken, Ozyme BLE906001) at a dilution of 1:250 and anti-Upk III (Rabbit, Abcam, ab157801) at a dilution of 1:800 in MaxBlock, 0.3% Triton X100.

    Techniques: Expressing, Infection, Plasmid Preparation, Immunofluorescence, Staining, MANN-WHITNEY, Mutagenesis

    a-i. Immunofluorescence staining of pH2AX on paraffin-embedded bladder sections 24 hours after PBS inoculation (a-c), or UTI89 wild-type (d-f) or UTI89Δ clbP (g-i) infection. The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, grey = phase contrast. Yellow arrows highlight bacteria, white dotted lines represent the basal membrane of the urothelium. Scale bar = 10 μm. The experiment was repeated twice on 5 to 8 mice, other representative images are shown in . j-l. pH2AX positive nuclei (j), Krt14 positive cells (k) and Krt14 and pH2AX positive cells (l) were counted objectively using Fiji and CellProfiler softwares on the confocal images from large mosaic of bladder sections. Each dot represents one mouse bladder. Mann Whitney U-test performed between UTI89 wild-type and UTI89 Δ clbP . m-t. Paraffin-embedded bladders sections were immuno-stained for pH2AX and Krt14 24 hours post infection with UTI89 wild-type (m-p) or UTI89Δ clbP (q-t). The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, green = Krt14. Cells positive for both Krt14 and pH2AX are circled in pink. White dotted lines highlight the basal membrane of the urothelium. Scale bar = 10 μm. Other representative images are shown in .

    Journal: PLoS Pathogens

    Article Title: Uropathogenic E. coli induces DNA damage in the bladder

    doi: 10.1371/journal.ppat.1009310

    Figure Lengend Snippet: a-i. Immunofluorescence staining of pH2AX on paraffin-embedded bladder sections 24 hours after PBS inoculation (a-c), or UTI89 wild-type (d-f) or UTI89Δ clbP (g-i) infection. The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, grey = phase contrast. Yellow arrows highlight bacteria, white dotted lines represent the basal membrane of the urothelium. Scale bar = 10 μm. The experiment was repeated twice on 5 to 8 mice, other representative images are shown in . j-l. pH2AX positive nuclei (j), Krt14 positive cells (k) and Krt14 and pH2AX positive cells (l) were counted objectively using Fiji and CellProfiler softwares on the confocal images from large mosaic of bladder sections. Each dot represents one mouse bladder. Mann Whitney U-test performed between UTI89 wild-type and UTI89 Δ clbP . m-t. Paraffin-embedded bladders sections were immuno-stained for pH2AX and Krt14 24 hours post infection with UTI89 wild-type (m-p) or UTI89Δ clbP (q-t). The individual fluorescence channel images are shown in grayscale. Merged images: blue = DNA, magenta = pH2AX, green = Krt14. Cells positive for both Krt14 and pH2AX are circled in pink. White dotted lines highlight the basal membrane of the urothelium. Scale bar = 10 μm. Other representative images are shown in .

    Article Snippet: Slices were stained with anti-pH2AX (Rabbit, Cell Signalling S9718; or Mouse, Millipore #O5-636) at a dilution of 1:200, anti-Krt14 antibodies (Chicken, Ozyme BLE906001) at a dilution of 1:250 and anti-Upk III (Rabbit, Abcam, ab157801) at a dilution of 1:800 in MaxBlock, 0.3% Triton X100.

    Techniques: Immunofluorescence, Staining, Infection, Fluorescence, MANN-WHITNEY