Structured Review

Millipore mouse anti pan cadherin
A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting <t>using</t> <t>antibodies</t> against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and <t>pan-Cadherin</t> (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.
Mouse Anti Pan Cadherin, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti pan cadherin/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti pan cadherin - by Bioz Stars, 2024-07
86/100 stars

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1) Product Images from "Increased interaction between connexin43 and microtubules is critical for glioblastoma stem-like cell maintenance and tumorigenicity"

Article Title: Increased interaction between connexin43 and microtubules is critical for glioblastoma stem-like cell maintenance and tumorigenicity

Journal: bioRxiv

doi: 10.1101/2024.01.26.576347

A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting using antibodies against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and pan-Cadherin (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.
Figure Legend Snippet: A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting using antibodies against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and pan-Cadherin (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.

Techniques Used: Cell Culture, Microscopy, Derivative Assay, Western Blot, Staining, Immunofluorescence


Structured Review

Santa Cruz Biotechnology mouse monoclonal anti pan cadherin
Mouse Monoclonal Anti Pan Cadherin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti pan cadherin/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal anti pan cadherin - by Bioz Stars, 2024-07
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Structured Review

Santa Cruz Biotechnology mouse monoclonal anti pan cadherin
Mouse Monoclonal Anti Pan Cadherin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti pan cadherin/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal anti pan cadherin - by Bioz Stars, 2024-07
86/100 stars

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rabbit anti mouse pan cadherin polyclonal antibody  (Danaher Inc)


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    Danaher Inc rabbit anti mouse pan cadherin polyclonal antibody
    Rabbit Anti Mouse Pan Cadherin Polyclonal Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse pan cadherin polyclonal antibody/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse pan cadherin polyclonal antibody - by Bioz Stars, 2024-07
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    rabbit anti mouse pan cadherin polyclonal antibody  (Abcam)

     
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    Abcam rabbit anti mouse pan cadherin polyclonal antibody
    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) <t>pan-Cadherin</t> ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).
    Rabbit Anti Mouse Pan Cadherin Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse pan cadherin polyclonal antibody/product/Abcam
    Average 86 stars, based on 1 article reviews
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    rabbit anti mouse pan cadherin polyclonal antibody - by Bioz Stars, 2024-07
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    1) Product Images from "Transglutaminase 2 Facilitates Murine Wound Healing in a Strain-Dependent Manner"

    Article Title: Transglutaminase 2 Facilitates Murine Wound Healing in a Strain-Dependent Manner

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms241411475

    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) pan-Cadherin ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).
    Figure Legend Snippet: TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) pan-Cadherin ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).

    Techniques Used: Expressing, Generated, Isolation, Western Blot, Quantitation Assay

    mouse anti pan cadherin monoclonal antibody  (Millipore)

     
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    Millipore mouse anti pan cadherin monoclonal antibody
    Mouse Anti Pan Cadherin Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pan cadherin monoclonal antibody/product/Millipore
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    mouse anti pan cadherin monoclonal antibody  (Millipore)

     
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    Millipore mouse anti pan cadherin monoclonal antibody
    Mouse Anti Pan Cadherin Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pan cadherin monoclonal antibody/product/Millipore
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    Merck & Co monoclonal mouse anti pan cadherin antibody
    Monoclonal Mouse Anti Pan Cadherin Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti pan cadherin antibody/product/Merck & Co
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    monoclonal mouse anti pan cadherin antibody  (Millipore)

     
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    Millipore monoclonal mouse anti pan cadherin antibody
    Monoclonal Mouse Anti Pan Cadherin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti pan cadherin antibody/product/Millipore
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    Millipore mouse anti pan cadherin
    Mouse Anti Pan Cadherin, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mouse anti pan cadherin
    A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting <t>using</t> <t>antibodies</t> against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and <t>pan-Cadherin</t> (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.
    Mouse Anti Pan Cadherin, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pan cadherin/product/Millipore
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    Santa Cruz Biotechnology mouse monoclonal anti pan cadherin
    A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting <t>using</t> <t>antibodies</t> against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and <t>pan-Cadherin</t> (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.
    Mouse Monoclonal Anti Pan Cadherin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti pan cadherin/product/Santa Cruz Biotechnology
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    Danaher Inc rabbit anti mouse pan cadherin polyclonal antibody
    A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting <t>using</t> <t>antibodies</t> against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and <t>pan-Cadherin</t> (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.
    Rabbit Anti Mouse Pan Cadherin Polyclonal Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse pan cadherin polyclonal antibody/product/Danaher Inc
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    Abcam rabbit anti mouse pan cadherin polyclonal antibody
    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) <t>pan-Cadherin</t> ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).
    Rabbit Anti Mouse Pan Cadherin Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse pan cadherin polyclonal antibody/product/Abcam
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    Millipore mouse anti pan cadherin monoclonal antibody
    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) <t>pan-Cadherin</t> ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).
    Mouse Anti Pan Cadherin Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co monoclonal mouse anti pan cadherin antibody
    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) <t>pan-Cadherin</t> ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).
    Monoclonal Mouse Anti Pan Cadherin Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti pan cadherin antibody/product/Merck & Co
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    Millipore monoclonal mouse anti pan cadherin antibody
    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) <t>pan-Cadherin</t> ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).
    Monoclonal Mouse Anti Pan Cadherin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting using antibodies against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and pan-Cadherin (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.

    Journal: bioRxiv

    Article Title: Increased interaction between connexin43 and microtubules is critical for glioblastoma stem-like cell maintenance and tumorigenicity

    doi: 10.1101/2024.01.26.576347

    Figure Lengend Snippet: A) VTC-001, VTC-034, and VTC-037 GSCs were cultured in stem cell media as gliospheres or as adherent cells, or differentiated for 1 to 3 days in medium containing 10% FBS, and observed by phase-contrast microscopy. Scale bar: 200 μm. B) VTC-001, VTC-034, and VTC-037 cultured as GSC-derived gliospheres (sph), adherent GSCs (adh), or differentiated cells after addition of 10% FBS for 1, 2, 3, and 7 days, were lysed and analyzed by immunoblotting using antibodies against CD133, Olig2, and GAPDH as loading control. C) VTC-001, VTC-034, and VTC-037 GSCs were differentiated or not for 24 h, and cell lysates were analyzed by immunoblotting for Cx43, and GAPDH as loading control. D) VTC-001, VTC-034, and VTC-037 GSCs in culture were fixed and immunostained using antibodies against Cx43 (red), and pan-Cadherin (pan-Cad – green) to stain cell borders. Confocal immunofluorescence was used to observe Cx43 subcellular localization in the cytoplasm and at the cell borders. DAPI was used to stain nuclei. Scale bar: 20 μm.

    Article Snippet: Primary antibodies rabbit anti-Cx43, mouse anti-pan Cadherin, or mouse anti-α-tubulin (MilliporeSigma) were diluted in blocking buffer and labeling was performed overnight at 4°C.

    Techniques: Cell Culture, Microscopy, Derivative Assay, Western Blot, Staining, Immunofluorescence

    TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) pan-Cadherin ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).

    Journal: International Journal of Molecular Sciences

    Article Title: Transglutaminase 2 Facilitates Murine Wound Healing in a Strain-Dependent Manner

    doi: 10.3390/ijms241411475

    Figure Lengend Snippet: TG2 expression levels are higher in 129 than in B6 mice. ( A , B ) cDNA generated from total RNA isolated from 129 or B6 TG2 +/+ and TG2 −/− unwounded skin samples ( n = 3 per genotype) ( A ) or skin samples on day 2 post-wounding ( n = 3 per genotype) ( B ) was examined in triplicate for the abundance of mRNAs for TG family members: Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; ***, p < 0.001 for individual post hoc Bonferroni test results from two-way ANOVA comparing 129 TG2 +/+ with B6 TG2 +/+ samples. ( C – F ) Representative western blots ( C , E ) and quantitation ( D , F ) of TG2 abundance relative to the reference proteins (expression level unaffected by genotype) pan-Cadherin ( C , D ) or GAPDH ( E , F ) in membrane or cytosolic fractions, respectively, of 129 or B6 TG2 +/+ and TG2 −/− MEF lysates ( n = 4 experiments).

    Article Snippet: Rabbit anti-mouse GAPDH polyclonal antibody (1:5000; Abcam) and rabbit anti-mouse pan-Cadherin polyclonal antibody (1:2500; Abcam) served as the most suitable reference protein for loading (expression unaffected by genotype) for cytoplasmic and membrane fractions, respectively.

    Techniques: Expressing, Generated, Isolation, Western Blot, Quantitation Assay