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mouse anti p histone3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti p histone3
    Mouse Anti P Histone3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti p histone3/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    mouse anti p histone3 - by Bioz Stars, 2025-04
    96/100 stars

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    SNAP29 depletion in neuroepithelial stem (NES) cells causes Golgi apparatus (GA) and spindle alteration and formation of micronuclei. (A) Maximal confocal projections of NES cells treated and stained as indicated. Depleted NES cells display GA fragmentation. (B) Quantification of the number of Giantin-positive objects. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test. (C) Maximal confocal projections of NES cells treated and stained to detect α-tubulin and <t>p-Histone3.</t> The depleted NES cells in metaphase show an altered mitotic spindle. The arrows indicate spindle poles. (D) Maximal confocal projections of NES cells treated and stained as indicated. The depleted NES cells possess several micronuclei (arrows). (E) Quantification of the percentage of cells with at least one micronucleus. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test.
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    mouse anti p histone3  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc mouse anti p histone3
    SNAP29 depletion in neuroepithelial stem (NES) cells causes Golgi apparatus (GA) and spindle alteration and formation of micronuclei. (A) Maximal confocal projections of NES cells treated and stained as indicated. Depleted NES cells display GA fragmentation. (B) Quantification of the number of Giantin-positive objects. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test. (C) Maximal confocal projections of NES cells treated and stained to detect α-tubulin and <t>p-Histone3.</t> The depleted NES cells in metaphase show an altered mitotic spindle. The arrows indicate spindle poles. (D) Maximal confocal projections of NES cells treated and stained as indicated. The depleted NES cells possess several micronuclei (arrows). (E) Quantification of the percentage of cells with at least one micronucleus. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test.
    Mouse Anti P Histone3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti p histone3/product/Cell Signaling Technology Inc
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    SNAP29 depletion in neuroepithelial stem (NES) cells causes Golgi apparatus (GA) and spindle alteration and formation of micronuclei. (A) Maximal confocal projections of NES cells treated and stained as indicated. Depleted NES cells display GA fragmentation. (B) Quantification of the number of Giantin-positive objects. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test. (C) Maximal confocal projections of NES cells treated and stained to detect α-tubulin and p-Histone3. The depleted NES cells in metaphase show an altered mitotic spindle. The arrows indicate spindle poles. (D) Maximal confocal projections of NES cells treated and stained as indicated. The depleted NES cells possess several micronuclei (arrows). (E) Quantification of the percentage of cells with at least one micronucleus. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus

    doi: 10.3389/fcell.2021.637565

    Figure Lengend Snippet: SNAP29 depletion in neuroepithelial stem (NES) cells causes Golgi apparatus (GA) and spindle alteration and formation of micronuclei. (A) Maximal confocal projections of NES cells treated and stained as indicated. Depleted NES cells display GA fragmentation. (B) Quantification of the number of Giantin-positive objects. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test. (C) Maximal confocal projections of NES cells treated and stained to detect α-tubulin and p-Histone3. The depleted NES cells in metaphase show an altered mitotic spindle. The arrows indicate spindle poles. (D) Maximal confocal projections of NES cells treated and stained as indicated. The depleted NES cells possess several micronuclei (arrows). (E) Quantification of the percentage of cells with at least one micronucleus. The median with interquartile range is shown, and the p -value is obtained by Mann–Whitney test.

    Article Snippet: The following primary antibodies were used: chicken anti-GFP 1:1,000 (Abcam), mouse anti-Golgin97 1:100 (Invitrogen), rabbit anti-Giantin 1:1,000 (Bio Legend), rabbit anti-GM130 1:1,000 (cell signaling), rabbit anti-βCOP 1:1,000 (Invitrogen), mouse anti-SEC31 1:100 (BD Biosciences), rabbit anti-VAPB 1:1,000, rabbit anti-KDEL receptor (KDELR) 1:200 (a gift from A. DeMatteis), mouse anti-ERGIC53 1:1,000, rabbit anti-SEC22B 1:1,000 (SYSY), rabbit anti-STX5 1:1,000 (SYSY), mouse anti-STX18 (Santacruz); rabbit anti-NSF 1:1,000 (SYSY), DAPI 1:1,000 (Sigma); mouse anti-p-Histone3 1:2,000 (Abcam), rat anti-α-tubulin 1:100 (AbD Serotec), and mouse anti-Nestin 1:200 (R&D Systems Inc., #MAB1259).

    Techniques: Staining, MANN-WHITNEY